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Journal : Natural B

Radiation Effects on Decreased Estrogen with Isoflavone Consumption to Prevent Early Menopause in Radiation Therapy Yeni Cahyati; Didik Rahadi Santoso; Unggul P Juswono
Natural B, Journal of Health and Environmental Sciences Vol 2, No 2 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1456.776 KB) | DOI: 10.21776/ub.natural-b.2013.002.02.2

Abstract

Radiation effect is one of which is the reduction in estrogen. Gamma rays are ionizing radiation that can lead to cell damage due to free radicals produced when the radiation interacts with cells. The level of cell damage can be minimized by antioxidants. Antioxidants used is the isoflavones of soy milk, so it can be know the effects of radiation on the decline in estrogen that accompanied the consumption of isoflavones to prevent early menopause in radiation therapy.Radiation exposure are given fractionally. The dose was 1,5 Gy in once exposure and given every 3 days up to a maximum dose of the treatment groups. Consumption of soy milk to the mice done orally. Blood was drawn and serum was made. Serum estrogen levels will be tested by using ELISA.The results showed that the ovarium disfunction and stress experienced by mice (Mus musculus) caused by radiation exposure. Maximum results in the consumption of isoflavones on estrogen levels the subject to radiation exposure is 44 mg/day and 131 mg/day of soy milk. This isoflavone dose increase in estrogen levels maximumly. The increase was the response given by the body's immune system of mice due to start reacting and isoflavones are phytoestrogens.
The Combination Effect of Vitamin C and E on the Number of Hemoglobin, Leukocyte and Platelet Post-Irradiation of Gamma Ray Roni Prisyanto; Didik R Santoso; Unggul P Juswono; Yeni Cahyati
Natural B, Journal of Health and Environmental Sciences Vol 2, No 3 (2014)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.807 KB) | DOI: 10.21776/ub.natural-b.2014.002.03.14

Abstract

Radiotherapy is an alternative treatment for cancer patients to inhibit and destroy cancer cells due to the ionization. Gamma rays ionize not only cancer cells but also damage healthy cells due to precence of free radicals produced by ionizing gamma rays when interacting with biological material. The decrease in blood cells as ie hemoglobin, leukocytes and platelets caused free radicals can be minimized by administering antioxidant vitamins C and E. This study was conducted to determine the effects of gamma radiation exposure to the decrease in hemoglobin blood cells, leukocytes and platelets of mice after administration of a combination of antioxidant vitamins C and E in five variations, namely 10 mg dose: 1μl, 15 mg: 1.5 μl, 20 mg: 2 μl, 25 mg: 2.5 μl, 30 mg: 3 μl. Exposure of gamma radiation was given fractionation ranging from 200 rads to 1000 rads. Blood was taken from the heart for hemoglobin, leukocytes and platelets tests. The results showed that the administration of vitamins C and E may reduce the decline in hemoglobin levels, leukocytes, platelets resulting from exposure to gamma radiation when compared to the control group or without vitamin.
Insecticide Effects on Membrane Potential of Catfish Egg Cell (Clarias batrachus) Unggul Punjung Juswono; Kusharto Kusharto; Yeni Cahyati; Risalatul Latifah
Natural B, Journal of Health and Environmental Sciences Vol 1, No 3 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (373.853 KB) | DOI: 10.21776/ub.natural-b.2012.001.03.9

Abstract

Pollution has been occured in our environment due to daily life waste, industrial and using of peptiside in agroindustrial farm. Over usuage and dose of peptiside could be harmfull to the farm environment especially for fish farm. Measurement of membrane potential of fish egg cells is a simple metode to investigate water polution level. Membrane potential of fish egg cells can be measured using microelectrode probe which is connected to an electrometer. The changing of membrane potential value indicate the level of water polution. Variation of peptiside concentrations cause the changing of potential membrane value. Increasing of peptiside concentration cause decreasing of potential membrane. Its may due to some blocking of channel and other protein by peptiside molecule so the permeability of membrane to ions is decrease. The results of our experiment show that the increasing of peptiside concentration cause decreasing of the membrane potential value. For peptiside concentration of  0.4% decrease potential membrane to -28 ± 5 mV. It means that the increasing peptiside concentration cause significanly decrease in potential membrane which may be used for prediction of water polution level.