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All Journal SAINS DAN MATEMATIKA JURNAL KIMIA SAINS DAN APLIKASI Law, Development and Justice Review
Khairul Anami
Chemistry Department, Faculty Of Sciences And Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang
Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering Law, Crime, Criminology & Criminal Justice Mathematics

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Journal : SAINS DAN MATEMATIKA

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Anthocyanin Identification of Methanol-HCl Extract Active Fraction in Rosella (Hibiscus Sabdariffa. L) and Its Potential as Xanthine Oxidase Inhibitor Pratiwi Puji Lestari; Dewi Kusrini; Khairul Anam
JURNAL SAINS DAN MATEMATIKA Volume 22 Issue 3 Year 2014
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

The active fraction of methanol-HCl extract of Roselle flower (Hibiscus sabdariffa. L) has been identified and its inhibitor xanthine oxidase potential tested. The aim of this study was to compare the inhibitory activity of xanthine oxidase from methanol-HCl extract and its fractionation results and to identify the chemical component of Roselle flower’s methanol-water active fraction which had xanthine oxidase inhibition activity. The chemical component identification of Roselle flower was preceded by extraction and fractionation. The types of chemical compound contents were identified by the spotting appearance, UV-Vis and IR spectroscopy. The quantification of chemical compound was carried out by TLC Scanner and xanthine oxidase inhibitory activity was tested in vitro. From this research, it was obtained the methanol-HCl extract yield of 4%. The Roselle methanol-HCl extract has the ability to inhibit activity of xanthine oxidase (IC50) was 0.64 ppm which was preponderant than the fractionation result. The 2nd fraction was the most active to inhibit the xanthine oxidase activity compared to the 1st and 3rd fraction. The main components of 2nd fraction were isolates A (Rf 0.9) and isolates D (Rf=0.64) which were expected as the group of anthocyanin. The relative level of isolate A and D were 4.67% and 24.24% respectively.
Isolasi, Karakterisasi dan Uji Aktivitas Antioksidan Flavonoid dari Ekstrak Air Kulit Batang Ketapang Kencana (Terminalia muelleri Benth.) Wiwit Wulan Yuniati; Khairul Anam; Dewi Kusrini
JURNAL SAINS DAN MATEMATIKA Volume 20 Issue 3 Year 2012
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

Telah diisolasi, karakterisasi dan uji aktivitas antioksidan senyawa flavonoid dari ekstrak air kulit batang tumbuhan Ketapang Kencana (Terminalia muelleri Benth.). Identifikasi jenis flavonoid dilakukan dalam lima tahap, tahap pertama adalah preparasi serta ekstraksi sampel menggunakan metode infudasi, tahap kedua, penapisan fitokimia serbuk ekstrak air, tahap ketiga, isolasi senyawa flavonoid meliputi pemisahan menggunakan kromatografi kolom, hidrolisis asam dan ekstraksi fraksi serta uji kemurnian isolat, tahap keempat, karakterisasi dan elusidasi isolat murni flavonoid hasil isolasi secara spektroskopi menggunakan spektrometer Infra merah (IR), spektrofotometer UV-Vis dengan penambahan pereaksi geser, spektrometer 1H-NMR serta 13C-NMR dan tahap kelima yaitu uji aktivitas antioksidan isolat menggunakan metode DPPH. Isolat berupa serbuk kuning dengan rendemen 0,2% dan memiliki titik lebur 306-307,5 oC. Karakterisasi secara spektrometri menunjukkan bahwa isolat adalah Kuersetin. Uji aktivitas antioksidan isolat kuersetin memiliki nilai (IC50) 257,23 ppm.   Keywords: Terminalia muelleri Benth., flavonoid, kuersetin, DPPH
Co-Authors Agustina L.N. Aminin Alik Kandhita Febriani Ana Silviana Bambang Cahyono Dewi Kusrini Eka Vany Anggraeni Handojo Djoko Waloejo Hendri Widiyandari Ika Pratiwi Khosimah Adinata Irma Yunitasari Ismiyarto Ismitarto Jaka Dwi Pamungkas Mitha Dea Anggistia Ngadiwiyana M.Si. S.Si. Pratiwi Puji Lestari Veronika Adelina Hartini Wiwit Wulan Yuniati