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All Journal Animal Production : Indonesian Journal of Animal Production
T Susilawati
Bagian Produksi Ternak Fakultas Peternakan UB, Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Success Rate of Pregnancy and Gender Accuracy as The Result of Artificial Insemination by Using Froozen Semen Sexing on Ongole Hybrid Cattle Susilawati, T
ANIMAL PRODUCTION Vol 7, No 3 (2005): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

This research is meant to recognize the difference among pregnancy rate, service per-conseption (S/C) and pregnancy rate of the first Artificial Insemination  or conseption rate (CR) and also calf sex accuracy which is born from Ongole hybrid cattle (PO) through Artificial Insemination with froozen semen deposition resulted from different consentration sexing at the position 4 (korpus uteri) and 4+ (bottom end of kornua uteri).  The result of this research shows that Artificial Insemination at the position 4 and 4+ with spermatozoa X: pregant 60% (from 10 cattles); CR for each 50%; S/C : 2.00 and 1.83; IB with spermatozoa Y equivalent 80% while S/C 1.42 and 1.25. (Animal Production 7(3): 161-167 (2005) Key Words : Frozen sexing sperm, Service per conception, Calving rate
Biochemistry Characterization of Inhibin from Goat Granulosa Cells Siregar, TN; Aulanni’am, Aulanni’am; Susilawati, T; Riady, G; Hamdan, Hamdan; Armansyah, T
ANIMAL PRODUCTION Vol 8, No 2 (2006): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The objective this research is to study the isolation and characterization of inhibin from goat granulosa cells.  Sample of ovary collected from abbatoir of Sukun in Malang. Minimum diameter of follicle that can be used in this research is 3 mm. Granulosa cells Oocytes collection was done by aspiration method, and granulosa cells separate from oosit mechanically. Biochemistry characterization of inhibin was done by technique of SDS PAGE, biuret and PAS. The result of research confirmed that inhibin has molecular 32 kDa. Composition of protein, carbohydrate, and glikoprotein inhibin was 3.636±0,002; 2.569±0,002; and 6.205±0.016 mg/mL, respectively. (Animal Production 8(2): 100-107 (2006) Key Words: Inhibin, granulosa cells, oocytes, follicle
Co-Authors Aulanni'am, Aulanni'am G Riady, G Hamdan Hamdan N Isnaini Ratnawati, Dian T Armansyah, T TN Siregar, TN