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All Journal Animal Production : Indonesian Journal of Animal Production
TN Siregar, TN
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Biochemistry Characterization of Inhibin from Goat Granulosa Cells Siregar, TN; Aulanni’am, Aulanni’am; Susilawati, T; Riady, G; Hamdan, Hamdan; Armansyah, T
ANIMAL PRODUCTION Vol 8, No 2 (2006): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The objective this research is to study the isolation and characterization of inhibin from goat granulosa cells.  Sample of ovary collected from abbatoir of Sukun in Malang. Minimum diameter of follicle that can be used in this research is 3 mm. Granulosa cells Oocytes collection was done by aspiration method, and granulosa cells separate from oosit mechanically. Biochemistry characterization of inhibin was done by technique of SDS PAGE, biuret and PAS. The result of research confirmed that inhibin has molecular 32 kDa. Composition of protein, carbohydrate, and glikoprotein inhibin was 3.636±0,002; 2.569±0,002; and 6.205±0.016 mg/mL, respectively. (Animal Production 8(2): 100-107 (2006) Key Words: Inhibin, granulosa cells, oocytes, follicle
ESTROUS OF GOATS UNDERGONE SUPEROVULATION INDUCTION WITH ANTI-INHIBIN Siregar, TN; Armansyah, T
ANIMAL PRODUCTION Vol 11, No 1 (2009): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Estrous performance of goats underlying superovulation induction with anti-inhibin was done using anti-inhibin induction on rabbit. Twenty four goats used in this research that divided four groups namely P0 (control), P1 (immunization at days of 4 cycle), P2 (immunization at days of 9 cycle)  and P3 (immunization at days of 13 cycle). Immunization on groups was done with injection of 500 ?g antibody against inhibin after synchronize before that. Estrous synchronization using injection 0.5 ml cloprostenol  (EstronTM, Bioveta) two times with interval 10 days. Twenty four hours after immunization, all of goats injection of cloprostenol with same doses. Estrous observation was done after injection of cloprostenol later three times a days. Collection of blood was done at estrous (to analyze of estradiol concentration) and at days of 7 cycle (to analyze of progesterone concentration). All f goats after treatment showed estrous behavior as swelling around the vulva and redness, a thin mucous discharge from vulva, social behaviors, and showed mounted. Awal of estrous on P0, P1, P2, and P3 groups were  35.00+7.01; 28.67+4,50; 27.67+4.76; and 29.67+5.86 hours, respectively and revealed no significant difference between control and treatment groups. Duration of estrous on P0, P1, P2, and P3 were 36.67+3.27; 49.33+3.20; 50.33+10.23; and 53.67+11.96 hours, respectively and revealed significant difference (P<0.05) between P2 and P3 with P0 and P1. Length of cycle on  P0, P1, P2, and P3 groups were  20.33+1.75; 19.33+0.82; 19.33+2.50; and 20.83+2.56, respectively and revealed no significant difference between control and treatment groups. (Animal Production 11(1): 34-39 (2009) Key Words: anti-inhibin, goats, superovulation, synchronization
Co-Authors Aulanni'am, Aulanni'am G Riady, G Hamdan Hamdan T Armansyah, T T Susilawati