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All Journal AGRIVITA, Journal of Agricultural Science
Sakhidin Sakhidin
University of Jenderal Soedirman
Agriculture, Biological Sciences & Forestry

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PEROXIDASE ISOZYME IDENTIFICATION OF SOME RICE GENOTYPES IN M1 GENERATION UNDER DROUGHT STRESS LEVEL OF -0.03 MPa Herwibawa, Bagus; Haryanto, Totok Agung Dwi; Sakhidin, Sakhidin
AGRIVITA, Journal of Agricultural Science Vol 36, No 3 (2014)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

The effort to fulfill the need of rice through the improvement of dryland productivity can be viewed as a more environmentally-friendly way. This research used 36 rice genotypes in M1 generation that were grown hydrophonically under drought stress level of -0.03 MPa. The identifications were conducted based on peroxidase isozyme marker. The isozyme patterns in zymogram were binary-coded by visual scores for each genotype, based on the thickness and the number in the appearance of bands on certain migration distance. The migration distances were measured based on values of Rf. The similarity coefficients were calculated using Dice’s coefficient that were used to construct dendrogram using the UPGMA employing the SAHN from the NTSYSpc 2.02. The results showed that the most resistant genotype under drought stress was R-4, and the genetic relationships among the genotypes were divided into two main groups, aromatic and non-aromatic group, in which some genotypes experienced the reduced levels of aromatic character (R-8 and R-9) and the drought resistance character (IU-2, IU-3, IU-4, IU-5, IU-6, IU-7, and IU-8), but there were some genotypes to able to improve the resistance under drought stress (R-2, R-3, R-5, R-6, R-7, IT-4, IT-5, and IT-7). Keywords:  drought resistance, gamma irradiation, peroxidase isozyme, rice, sodium azide
THE EFFECT OF GAMMA IRRADIATION AND SODIUM AZIDE ON GERMINATION OF SOME RICE CULTIVARS Herwibawa, Bagus; Haryanto, Totok Agung Dwi; -, Sakhidin
AGRIVITA, Journal of Agricultural Science Vol 36, No 1 (2014)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

Efforts to increase rice production through genetic improvement are often limited by the availability of natural diversity. That natural diversity can be improved through induced mutation. Selected characters can be observed since the germination phases, which may also indicate the plants survival under field conditions. Experimental design was arranged in split plot, with cultivars as main plot and mutagen doses as sub plot. The experiment consisted of 36 treatment combinations, with each treatment consisting of 10 replications. Data were recorded on percentage of first count, final count and germination rate. The data were analyzed using F-test by SAS 9.0 and mean separation was carried out by employing DMRT at 95 % (α = 5 %) of confidence level. The results showed that the best cultivar response for germination traits was Inpari 13, the best mutagen to build cultivar for germination traits was Gamma 150 Gy, and the best combination between cultivar and mutagen for germination traits was Inpago Unsoed 1 that was treated with Gamma 150 Gy. Keywords: gamma irradiation, germination, rice, sodium azide
THE EFFECT OF GAMMA IRRADIATION AND SODIUM AZIDE ON GERMINATION OF SOME RICE CULTIVARS Bagus Herwibawa; Totok Agung Dwi Haryanto; Sakhidin Sakhidin
AGRIVITA Journal of Agricultural Science Vol 36, No 1 (2014)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v36i1.360

Abstract

Efforts to increase rice production through genetic improvement are often limited by the availability of natural diversity. That natural diversity can be improved through induced mutation. Selected characters can be observed since the germination phases, which may also indicate the plants survival under field conditions. Experimental design was arranged in split plot, with cultivars as main plot and mutagen doses as sub plot. The experiment consisted of 36 treatment combinations, with each treatment consisting of 10 replications. Data were recorded on percentage of first count, final count and germination rate. The data were analyzed using F-test by SAS 9.0 and mean separation was carried out by employing DMRT at 95 % (α = 5 %) of confidence level. The results showed that the best cultivar response for germination traits was Inpari 13, the best mutagen to build cultivar for germination traits was Gamma 150 Gy, and the best combination between cultivar and mutagen for germination traits was Inpago Unsoed 1 that was treated with Gamma 150 Gy. Keywords: gamma irradiation, germination, rice, sodium azide
Co-Authors Bagus Herwibawa Bagus Herwibawa Bagus Herwibawa Totok Agung Dwi Haryanto Totok Agung Dwi Haryanto Totok Agung Dwi Haryanto