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All Journal Jurnal Fitopatologi Indonesia Journal of Degraded and Mining Lands Management AGRIVITA, Journal of Agricultural Science Kultivasi Jurnal Agrotek Tropika PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Totok Agung Dwi Haryanto
University of Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences

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PEROXIDASE ISOZYME IDENTIFICATION OF SOME RICE GENOTYPES IN M1 GENERATION UNDER DROUGHT STRESS LEVEL OF -0.03 MPa Herwibawa, Bagus; Haryanto, Totok Agung Dwi; Sakhidin, Sakhidin
AGRIVITA, Journal of Agricultural Science Vol 36, No 3 (2014)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The effort to fulfill the need of rice through the improvement of dryland productivity can be viewed as a more environmentally-friendly way. This research used 36 rice genotypes in M1 generation that were grown hydrophonically under drought stress level of -0.03 MPa. The identifications were conducted based on peroxidase isozyme marker. The isozyme patterns in zymogram were binary-coded by visual scores for each genotype, based on the thickness and the number in the appearance of bands on certain migration distance. The migration distances were measured based on values of Rf. The similarity coefficients were calculated using Dice’s coefficient that were used to construct dendrogram using the UPGMA employing the SAHN from the NTSYSpc 2.02. The results showed that the most resistant genotype under drought stress was R-4, and the genetic relationships among the genotypes were divided into two main groups, aromatic and non-aromatic group, in which some genotypes experienced the reduced levels of aromatic character (R-8 and R-9) and the drought resistance character (IU-2, IU-3, IU-4, IU-5, IU-6, IU-7, and IU-8), but there were some genotypes to able to improve the resistance under drought stress (R-2, R-3, R-5, R-6, R-7, IT-4, IT-5, and IT-7). Keywords:  drought resistance, gamma irradiation, peroxidase isozyme, rice, sodium azide
Potential of Biological Agents for Controlling Basal Rot Disease and Promoting Plant Growth in Shallot Djatmiko, Heru Adi; Khamidi, Tamrin; Haryanto, Totok Agung Dwi
Jurnal Fitopatologi Indonesia Vol. 18 No. 1 (2022): Januari 2022
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.18.1.9-18

Abstract

Potential of Biological Agents for Controlling Basal Rot Disease and Promoting Plant Growth in Shallot The use of biocontrol agents has been known as one component in integrated disease management for shallot. This study aimed to examine the effectiveness of three biocontrol agents (Bacillus subtilis strains B1 and B298, and nonpathogenic Fusarium oxysporum T14a) in suppressing the incidence of basal rot disease and promoting the growth of two shallot varieties (‘Bima Brebes’ and ‘Tajuk’) in the field. The experimental research was arranged in a factorial randomized block design consisting of two factors, i.e. type of biocontrol agent and shallot’s variety. It was shown that application of biocontrol agents prolongs the incubation period of the disease. All biocontrol agents increase plant resistance and reduce the incidence of basal rot disease. B. subtilis B1 and nonpathogenic F. oxysporum T14a had high efficacy, i.e 81.53% and 58.02%. Based on the observation of disease incidence and the area under the disease progression curve, it is known that var. ‘Tajuk’ is more susceptible to basal rot disease than var. ‘Bima Brebes’. Furthermore, the analysis showed that all biocontrol agents were able to increase the percentage of germination, leaf area index, plant growth rate, total chlorophyll in leaves and productivity. The highest percent increase over control in productivity was obtained by B. subtilis B1 (45.45%) followed by nonpathogenic F. oxysporum T14a (37.88%) and B. subtilis B298 (28.79%). Two of the three biocontrol agents tested, i.e. B. subtilis B1 and nonpathogenic F. oxysporum T14a are potential agents for controlling basal rot disease in shallots because they have good ability to increase plant growth and productivity.
Genetic parameters of yield component and yield in M1 rice (Oryza sativa L.) generation irradiated with gamma-ray Riyanto, Agus; Oktaviani, Eka; Wulansari, Nur Kholida; Haryanto, Totok Agung Dwi
Kultivasi Vol 23, No 2 (2024): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v23i2.55129

Abstract

High-yielding varieties are the primary determinant of the success of increasing Indonesia's rice production. Plant breeding for new high-yielding varieties is possible through mutation induction and selecting desired traits. The effectiveness and efficiency of selection require comprehensive genetic parameters. This study aimed to study the genetic diversity, heritability, genetic advance, and relationship between yield components and yield in the M1 generation of mutant rice irradiated with gamma ray. This research used a factorial randomized complete block design, involving gamma irradiation doses of 100 Gy, 150 Gy, and 200 Gy, as the first factor; and the second factor was the rice genotype: UnsoedBDBP, UnsoedBDIU, UnsoedBPIU, UnsoedIUBD, and UnsoedIBP. Each treatment was repeated three times. Results showed that traits with broad genetic variation were tiller number plant-1, grain number panicle-1, and grain yield plant-1. High heritability and genetic advance values were found in plant height, grain number panicle-1, and yield plant-1, indicating that improvement in these traits can be achieved simply through selection methods. Panicle number plant-1 and filled grain percentage panicle-1 showed a unidirectional relationship and directly affected high values on yield plant-1. Therefore, these traits can be considered selection indicators for breeding high-yielding mutant rice.
Penerapan Pompa Air Tenaga Surya untuk Irigasi Sawah di Desa Pruwatan, Kabupaten Brebes, Jawa Tengah: Implementation of Solar Water Pumps for Rice Field Irrigation in Pruwatan Village, Brebes Regency, Central Java Sudarmaji, Arief; Haryanto, Totok Agung Dwi; Lestari, Sri; Mudmainah, Siti; Febriyono, Wahyu; Murdiantoro, Randi Adzin
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 8 (2025): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v10i8.8948

Abstract

Water shortages during the dry season are the main problem for rice farmers in Pruwatan Village, Brebes District, Central Java. Currently, many rice fields in Pruwatan Village are rain-fed. This condition has been caused by natural disasters since 2010, which have resulted in dams breaking and landslides in irrigation canal cliffs. To meet water needs, this community service activity has built a solar-powered pump installation to raise water from the Pemali River that flows through Pruwatan Village to be channeled into the irrigation canal. The solar-powered pump uses an off-grid model (independent electricity without a PLN network connection) and without batteries. The pump used is a submersible type. The electrical energy to drive the pump comes 100% from the installed solar panels. The main device of the solar-powered pump consists of a series of 10,000 Wp solar panels, two units of 3 HP built-in inverters, two units of 3 HP submersible pumps, and a safety circuit (MCB). The pumps used are two 3 HP submersible pumps installed in parallel. The pump raises water from the Pemali River to the irrigation channel about 120 meters away with an elevation of 30 meters. The built-in inverter has a short circuit safety device, over/under voltage and undervoltage detection, and easy and user-friendly operation.
Cadmium (Cd) heavy metal reduction in liquid and soil using indigenous soil bacteria isolated from Cd-contaminated paddy fields Hadi, Sapto Nugroho; Haryanto, Totok Agung Dwi; Tamad, Tamad; Ahadiyat, Yugi R.
Journal of Degraded and Mining Lands Management Vol. 12 No. 5 (2025)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2025.125.8561

Abstract

The challenge in Indonesian agriculture is the contamination of cadmium (Cd) in rice fields. Cadmium contamination in rice fields is a serious environmental and health problem. Reducing Cd contamination from rice fields is essential. One way is through the use of indigenous microbes. The study aimed to obtain indigenous bacteria from Cd-contaminated rice fields and determine their ability to reduce Cd contamination in liquid and soil media. Soil samples were taken from six locations of Cd-contaminated rice fields in Banyumas Regency, Central Java, Indonesia. The population of soil bacteria was calculated. Selected isolates were tested for their tolerance and ability to reduce Cd. Potential isolates were identified based on their 16S rDNA sequences. The results showed that the most prominent Cd-tolerant bacteria were found in soil samples from Ajibarang, with 1.8 × 106 CFU/g of soil. The study obtained five isolates with high tolerance to Cd, up to 100 ppm, and was able to reduce Cd by 45.58% to 70.02% in liquid media and from 0.92 ppm to 0.02 ppm in soil media. All bacterial isolates are non-pathogenic to plants. Isolate GML2, SRU2, and KBS1 were from Priestia megaterium, JTL1 was from Neobacillus ginsengisoli, and PLK1 was from Gottfriedia sp. The isolates have the potential to be used to reduce Cd contamination and remediate rice fields contaminated with Cd.
GROWTH RESPONSE OF 3 ACCESSORIES OF PORANG (Amorphophallus muelleri Blume) ON VARIATION ORGANIC FERTILIZER DOSAGE Tini, Etik Wukir; Nisa, Khoerotun; Haryanto, Totok Agung Dwi
Jurnal Agrotek Tropika Vol. 13 No. 1 (2025): JURNAL AGROTEK TROPIKA VOL 13, FEBRUARI 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i1.6370

Abstract

This study aims to examine the growth of 3 porang accessions at various organic fertilizer doses. The research is a field experiment on ex-maize land in Bobosan Village, Banyumas from September 2021 to January 2022. The treatment design was a split plot design with 2 factors. The first factor was the accession of porang consisting of 3 levels, namely the accessions of Banyumas, Madiun, and Semarang (main plot). The second factor is organic fertilizer doses consisting of 3 levels, namely 1; 1.5; and 2 kg/plant (subplot). The results showed that the Banyumas accession gave the best growth in stem diameter, plant height, and leaf area compared to the Madiun and Semarang accessions; the best fertilizer dose for porang growth has not been obtained; there is an interaction effect of fertilizer dose and porang accession on the number of leaves and plant height. Plant height and leaf number of Banyumas accessions were better at a dose of 2 kg/plant, Madiun accessions were better at a dose of 1.5 kg/plant, and Semarang accessions were better at a dose of 1 kg/plant; organic fertilizer 2 kg/plant given to the Banyumas accession produced the highest plant height and several leaves.
Estimation of Genetic Parameters for Rice Length and Shape of F2 Population Riyanto, Agus; Susanti, Dyah; Sugiyono, Sugiyono; Hidayat, Ponendi; Suprayogi, Suprayogi; Haryanto, Totok Agung Dwi
AGRIVITA Journal of Agricultural Science Vol 46, No 2 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i2.4086

Abstract

Rice length and shape are the important quality traits in rice that affect consumer acceptance and price. Rice length and shape can be improved through crosses followed by selection. Genetic parameters influence effective selection. The research objectives are to estimate genetic parameters for rice length and shape in the F2 population of Basmati Delta 9 x Koshihikari and Basmati Pakistan x Inpago Unsoed 1. F2 rice population from crosses of Basmati Delta 9 (extra-long, slender) x Koshihikari (short, round), Basmati Pakistan (extra-long, slender) x Inpago Unsoed 1 (medium, medium), and the parents are used for research material. The genotype is planted using an experimental design with no replication. Rice length and shape data were collected from each plant. The collected data is used to estimate the genetic parameters of rice length and shape. The results show that polygenes control rice length and shape. Transgressive segregation is obtained in the F2 population of Pakistan Basmati x Inpago Unsoed 1. The genetic diversity of rice length and shape is low, so reducing the selection intensity of these traits is suggested. The estimate of broad sense heritability and genetic gain of rice length and shape was high.
PEROXIDASE ISOZYME IDENTIFICATION OF SOME RICE GENOTYPES IN M1 GENERATION UNDER DROUGHT STRESS LEVEL OF -0.03 MPa Herwibawa, Bagus; Haryanto, Totok Agung Dwi; Sakhidin, Sakhidin
AGRIVITA Journal of Agricultural Science Vol 36, No 3 (2014)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v36i3.436

Abstract

The effort to fulfill the need of rice through the improvement of dryland productivity can be viewed as a more environmentally-friendly way. This research used 36 rice genotypes in M1 generation that were grown hydrophonically under drought stress level of -0.03 MPa. The identifications were conducted based on peroxidase isozyme marker. The isozyme patterns in zymogram were binary-coded by visual scores for each genotype, based on the thickness and the number in the appearance of bands on certain migration distance. The migration distances were measured based on values of Rf. The similarity coefficients were calculated using Dice’s coefficient that were used to construct dendrogram using the UPGMA employing the SAHN from the NTSYSpc 2.02. The results showed that the most resistant genotype under drought stress was R-4, and the genetic relationships among the genotypes were divided into two main groups, aromatic and non-aromatic group, in which some genotypes experienced the reduced levels of aromatic character (R-8 and R-9) and the drought resistance character (IU-2, IU-3, IU-4, IU-5, IU-6, IU-7, and IU-8), but there were some genotypes to able to improve the resistance under drought stress (R-2, R-3, R-5, R-6, R-7, IT-4, IT-5, and IT-7). Keywords:  drought resistance, gamma irradiation, peroxidase isozyme, rice, sodium azide
Co-Authors Agus Riyanto AHADIYAT YUGI R. Arief Sudarmaji, Arief Bagus Herwibawa Dyah Susanti Eka Oktaviani Etik Wukir Tini Febriyono, Wahyu Heru Adi Djatmiko Mudmainah, Siti Nisa, Khoerotun Ponendi Hidayat Randi Adzin Murdiantoro Sakhidin Sakhidin Sakhidin Sakhidin Sapto Nugroho Hadi Sri Lestari Sugiyono Sugiyono Suprayogi Suprayogi Tamad, Tamad Tamrin Khamidi Wulansari, Nur Kholida