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All Journal HAYATI Journal of Biosciences AGRIVITA, Journal of Agricultural Science JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Siti Muslimah Widyastuti
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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INFECTION PROCESS OF Fusarium oxysporum FUNGUS: A CAUSE OF DAMPING-OFF ON Acacia mangium’s SEEDLINGS Widyastuti, Siti Muslimah; Tasik, Susanti; Harjono, Harjono
AGRIVITA, Journal of Agricultural Science Vol 35, No 2 (2013)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

Fusarium oxysporum is the causal agent of damping-off disease. The fungus attacks seedlings of many plant species, including Acacia mangium. In order to effectively control the disease, detailed information on how the fungus infects seedlings of A. mangium and how the plant responds to the fungal infection is essentially needed. The objectives of this research were to investigate: (1) the infection process of F. oxysporumon seedlings of A. mangium, (2) the defence response of A. mangium seedling to infection by F. oxysporum. The fungal pathogen was identified, followed by performance of pathogenicity test. The infection process was followed by macroscopic observation as well as microscopic observation. The result indicated that fungal spore germination was observed at two-day post inoculation in planta. At four-daypost inoculation, hyphae of F. oxysporum had penetrated the collar root of A. mangium seedling via stomata aperture. In addition, fungal hyphae had grown intercellulary in to the vascular tissue. Correspondingly, hypersensitive response was also detected at the stomata aperture. However, this defence mechanism is not effective in stopping the fungus since F. oxysporum is a necrotropic pathogen. Moreover, accumulation of lignin, but not callose, was observed. Keywords:  Fusarium oxysporum, damping-off, Acacia mangium
Purification and Characterization of ?-1,3-Glucanase from the Antagonistic Fungus Trichoderma reesei SRI WAHYUNI BUDIARTI; SITI MUSLIMAH WIDYASTUTI; SEBASTIAN MARGINO
HAYATI Journal of Biosciences Vol. 16 No. 3 (2009): September 2009
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (111.886 KB) | DOI: 10.4308/hjb.16.3.115

Abstract

Trichoderma enzymes that inhibit fungal cell walls have been suggested to play an important role in mycoparasitic action against fungal root rot pathogen Ganoderma philippii. This experiment was aimed to purify and characterize the ?-1,3-glucanase of T. reesei. Extracellular ?-1,3-glucanase was produced by growing mycoparasite T. reesei isolate T13 in colloidal chitin and sucrose as carbon sources. The enzyme was then purified to its homogeneity by precipitation with ammonium sulfate, followed by gel filtration chromatography and chromatofocusing. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) 12% was used to confirm the purity of enzyme at each stage of preparation and to characterize purified protein. The results showed that T. reesei produced at least three extracellular ?-1,3-glucanases. Estimation of molecular weight based on SDS-PAGE 12% have three isoform of ?-1,3-glucanase were 90 kDa for ?-1,3-glucanase-I, 75 kDa for ?-1,3-glucanase-II, and 64 kDa for ?-1,3-glucanase-III. Their optimum pH and temperature were 5 and 50 oC, respectively. Key words: ?-1,3-glucanase, Trichoderma reesei, Ganoderma philippii
MEKANISME PARASITISME TRICHODERMA HARZIANUM TERHADAP FUSARIUM OXYSPORUM PADA SEMAI ACACIA MANGIUM Susanti Tasik; Siti Muslimah Widyastuti; Harjono .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 15 No. 1 (2015): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.196 KB) | DOI: 10.23960/j.hptt.11572-80

Abstract

Mechanism of parasitism of Trichoderma harzianum on Fusarium oxysporum on Acacia mangium seedlings. Fusarium oxysporum is one of the most important soil-borne fungi the causal agent of damping-off disease. Detailed information it needed to know how the pathogen can be inhibited by Trichoderma harzianum. The objective of this research was to investigate the inhibition mechanism of T. harzianum on F. oxysporum in vitro and in planta. Green Flourescent Protein (GFP) T. harzianum was used as biocontrol agent of F. oxysporum. An in vitro inhibition test of T. harzianum was performed using dual culture method. In the in planta inhibition tests, seedlings of A. mangium were applied with GFP T. harzianum two days before inoculation of F. oxysporum; GFP T. harzianum was simultaneously applied with F. oxysporum and GFP T. harzianum was applied two days after inoculation of F. oxysporum. The inhibition effect of T. harzianum GFP was observed at seven days incubation, indicated by attachment of T. harzianum to F. oxysporum hyphae. GFP T. harzianum hyphae covered the colonies of F. oxysporum at 12 days after incubation. The highest life percentage of A. mangium seedlings was found on the treatment of GFP T. harzianum two days before inoculation of F. oxysporum (82.22%), whereas the lowest life percentage was found on seedling applied with GFP T. harzianum two days after inoculation of F. oxysporum (64.44%).
Co-Authors Anak Agung Gede Sugianthara Harjono . Harjono Harjono Sebastian Margino SRI WAHYUNI BUDIARTI Susanti Tasik