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All Journal Agrotrop : Journal on Agriculture Science AGRIVITA, Journal of Agricultural Science Jurnal Agro
Loekas Soesanto
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Agriculture, Biological Sciences & Forestry

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ABILITY TEST OF SEVERAL ANTAGONISTS TO CONTROL POTATO BACTERIAL WILT IN THE FIELD Soesanto, Loekas; Manan, Abdul; Wachjadi, Muljo; Mugiastuti, Endang
AGRIVITA, Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

The research objective was to know ability of antagonistic microbes to control bacterial wilt on potato in the field. This research was carried out at Serang Village, Karangreja Subdistrict, Purbalingga Regency from June up to August 2012. The antagonist, originally isolated from potato field, was Bacillus sp. B2 and B4, and Pseudomonas sp. P19 and P20. Based on the research result, Pseudomonas P19 could control the disease on potato with delaying incubation period of 78.95%, suppressing disease intensity of 51.57%, decreasing final pathogenic population of 99.74%, and inducing plant resistance with increasing saponin, tannin, and glycoside content. However, the antagonist could not increase growth and yield of potato.  Keywords: antagonistic microbes, bacterial wilt, potato
Aplikasi Bacillus sp. untuk mengendalikan penyakit layu fusarium pada tanaman tomat Endang Mugiastuti; Abdul Manan; Ruth Feti Rahayuniati; Loekas Soesanto
Jurnal Agro Vol 6, No 2 (2019)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/5397

Abstract

Fusarium oxysporum merupakan penyebab penyakit layu fusarium yang dapat menurunkan produksi tomat. Pengendalian hayati dengan menggunakan bakteri antagonis seperti Bacillus sp. merupakan alternatif pengendalian yang potensial dan ramah lingkungan.  Penelitian bertujuan untuk mengetahui kemampuan Bacillus sp. dalam mengendalikan penyakit layu fusarium pada tanaman tomat di lapangan. Percobaan menggunakan rancangan acak kelompok dengan 5 perlakuan dan 5 ulangan, meliputi: kontrol, Bacillus sp. B42, Bacillus sp. B64, gabungan Bacillus sp. B42 dan B64, serta fungisida. Hasil pengujian menunjukkan bahwa Bacillus sp. B.64 merupakan bakteri antagonis terbaik dalam menekan intensitas penyakit  layu fusarium, karena dapat menunda masa inkubasi 15,76%, menekan intensitas penyakit 38,77%, meningkatkan kandungan fenol tanaman (tanin, saponin dan glikosida), serta meningkatkan pertumbuhan dan hasil tanaman dengan meningkatkan bobot kering tajuk 32,05%, bobot kering akar 15,23%, dan bobot buah per tanaman 46,48%.ABSTRACTFusarium oxysporum is the causal agent of fusarium wilt disease which decreases the production of tomatoes. Biological control managenement using bacterial antagonists is a potential alternative to prevent the infection of the disease. The aim of this research was to determine the ability of Bacillus sp. to control tomato fusarium wilt in the field. Randomized block design (RBD) experiment was used consisting of 5 treatments and 5 replications i.e. control, Bacillus sp. B42, Bacillus sp. B64, combination of Bacillus sp B42 + B64, and fungicide. The results showed that Bacillus sp. B64 was the best bacterial antagonist agent to control tomato wilt disease by delaying incubation period (15.76%), decreasing disease intensity (38.77%), increasing phenol compounds (tannin, saponin, glycosides) and improving plant growth and yield. Furthermore, the results showed the increasement of shoot dry weight to 32.05%, root dry weight to 15.23%, and yield to 46.48% as well.
KAJIAN BEBERAPA MEDIUM PENYERAP KMNO4 DAN SUHU TERHADAP PENYAKIT ANTRAKNOSA PADA PISANG KULTIVAR SUSU LEPAS PANEN KEMASAN PLASTIK POLIETILEN RONY AL AFGANI; LOEKAS SOESANTO; DARINI SRI UTAMI
Agrotrop : Journal on Agriculture Science Vol 1 No 2 (2011)
Publisher : Fakultas Pertanian Universitas Udayana

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Study of Several KMnO4 Absorbant Media and Temperature on Antracnose of Postharvest Susu Cultivar Banana Packaged in Polyethylene Plastic. A research was aimed to know the best KMnO4 absorbant media, temperature, and their combination on antracnose of postharvest susu cultivar banana packaged in polyethylene plastic was carried out at the Laboratory of Plant Disease, Faculty of Agriculture, Jenderal Soedirman University, Purwokerto. The banana with age of 3.5 months after flowering was taken from Sokawera Village, Somagede District, Banyumas Regency, Central Java. Split-Plot Design was used with the plastic. The main plot was room (25-28oC) and low (15-17oC) temperatures. The sub plot was without media and with or without inoculation, media of charcoal, coal, brick, cotton, and rockwool. Variable observed was incubation period, attact area, attact intensity, waste index, softy rate, and sugar content. Result of the research showed that the best media for absorbing KMnO4 was cotton. Low temperature could decrease softy rate of 64.75% and attact area of 92.78% compared to room one. Combination between cotton or rockwool and without inoculation at low temperature was the best treatment because of decreasing attact area ofColletotrichum musae and waste index for 100%. Inoculation of the fungus was not affect fungus attact in the KMnO4 and its combination with temperature. All treatments were not affect colour, aroma, and taste of the banana.
Field Application of Trichoderma Suspension to Control Cacao Pod Rot (Phytophthora palmivora) Rina Sriwati; Tjut Chamzurn; Loekas Soesanto; Munazhirah Munazhirah
AGRIVITA, Journal of Agricultural Science Vol 41, No 1 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i1.2146

Abstract

Cacao pod rot caused by Phytophthora palmivora, is an important disease and contributes significant disease losses to global cocoa production. This research objective was to determine the effect of Trichoderma harzianum and T. virens suspensions to cacao pod rot disease on the field. This research was carried out in Pulo Hagu village, Pidie Regency, Aceh, Indonesia from March to July 2017. The single pattern randomized block design was adopted to evaluate three treatments, i.e. without suspension (control), suspensions of T. Harzianum, and T. virens for eight replications. Each replication consisted of three of experimental units. The result showed that both of Trichoderma species contained only Alkaloid metabolite based on Phytochemical test. On the field, the application of T. harzianum suspension reduced the percentage of fruit infection and disease intensity for 48.57 %, 46.04 % at 12 weeks after application (WAA) respectively. Based on the percentage reduction in the area of the spot between the metabolites T. harzianum suspension and control and T. virens and control are 47.24 % and 27.46 % at 87 WAA respectively. In addition, T. virens suppressed the percentage of infected fruit and the intensity of infected fruit for 40.61 % and 38.02 % at 12 WAA.
Co-Authors Abdul Manan DARINI SRI UTAMI Endang Mugiastuti Muljo Wachjadi Munazhirah Munazhirah Rina Sriwati RONY AL AFGANI Ruth Feti Rahayuniati Tjut Chamzurn