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APPLICATION OF RAW SECONDARY METABOLITES FROM FOUR ENTOMOPATHOGENIC FUNGI AGAINST CHILLI DISEASE CAUSED BY VIRUSES Soesanto, Loekas; Prastyani, Noviana; Sri Utami, Darini; Manan, Abdul
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 20 No. 2 (2020): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j.hptt.220100-107

Abstract

Application of raw secondary metabolites from four entomopathogenic fungi against chilli disease caused by viruses. The purpose of this research was to investigate several kinds of raw secondary metabolits to decrease viral disease in chilli and inspect their side effect to plant growth. This research was conducted at experimental farm, Faculty of Agriculture, Jenderal Soedirman University from November 2018 to March 2019. The chilli seeds used for indicator plant were obtained from virus-symptomatic chilli. The raw secondary metabolites was collected from four microbial isolates used in this study, i.e. Metarhizium anisopliae, Beauveria bassiana (Papua isolate), Lecanicillium lecanii and B. bassiana Bio B10 (Jember isolate). The experiment was arranged in completely randomized design with five replications. Observation was performed on incubation period, disease intensity, AUDPC, germination percentage, plant height, number of leaves, and number of shoots. The result showed that raw secondary metabolites obtained from M. anisopliae gave the best capability to suppress disease development. Application of M. anisopliae raw secondary metabolites reduced incubation period, viral disease intensity as well as AUDPC in 34.22; 77.98 and 79.49%, respectively. The raw secondary metabolites of L. lecanii could increase percentage of germination, plant height, number of leaves, and number of shoots as 100; 38.96; 38.96 and 52.38%, respectively, compared to control.
KAJIAN BEBERAPA MEDIUM PENYERAP KMNO4 DAN SUHU TERHADAP PENYAKIT ANTRAKNOSA PADA PISANG KULTIVAR SUSU LEPAS PANEN KEMASAN PLASTIK POLIETILEN RONY AL AFGANI; LOEKAS SOESANTO; DARINI SRI UTAMI
Agrotrop : Journal on Agriculture Science Vol 1 No 2 (2011)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Study of Several KMnO4 Absorbant Media and Temperature on Antracnose of Postharvest Susu Cultivar Banana Packaged in Polyethylene Plastic. A research was aimed to know the best KMnO4 absorbant media, temperature, and their combination on antracnose of postharvest susu cultivar banana packaged in polyethylene plastic was carried out at the Laboratory of Plant Disease, Faculty of Agriculture, Jenderal Soedirman University, Purwokerto. The banana with age of 3.5 months after flowering was taken from Sokawera Village, Somagede District, Banyumas Regency, Central Java. Split-Plot Design was used with the plastic. The main plot was room (25-28oC) and low (15-17oC) temperatures. The sub plot was without media and with or without inoculation, media of charcoal, coal, brick, cotton, and rockwool. Variable observed was incubation period, attact area, attact intensity, waste index, softy rate, and sugar content. Result of the research showed that the best media for absorbing KMnO4 was cotton. Low temperature could decrease softy rate of 64.75% and attact area of 92.78% compared to room one. Combination between cotton or rockwool and without inoculation at low temperature was the best treatment because of decreasing attact area ofColletotrichum musae and waste index for 100%. Inoculation of the fungus was not affect fungus attact in the KMnO4 and its combination with temperature. All treatments were not affect colour, aroma, and taste of the banana.
Pemberdayaan Kelompok Wanita Tani Melalui Optimalisasi Pemanfaatan Pekarangan dengan Budidaya Sayuran Organik Dataran Rendah Berbasis Kearifan Lokal dan Berkelanjutan Endang Warih Minarni; Darini Sri Utami; Nur Prihatiningsih
JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) VOL. 1 NOMOR 2 SEPTEMBER 2017 JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat)
Publisher : Lembaga Publikasi Ilmiah dan Penerbitan (LPIP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (496.802 KB) | DOI: 10.30595/jppm.v1i2.1949

Abstract

The specific purpose of this activity is to increase the motivation of women in improving the quality of life and self-reliance in the family by optimizing the yard with organic vegetable farming in the lowlands based on Local Wisdom and Sustainable.  The strategy used was  the empowerment of target groups with the approach of Participatory Rural Appraisal, methods of education, training, demonstration plots, assistance and equipped with learning by doing techniques. The materials presented were: (1) garden  intensification technology with verticultur method, (2) organic vegetable cultivation technology based on liquid organic fertilizer, botanical pesticide and PGPR, (3) composting technology with enrichment of Trichoderma harzianum (Tricho-compos) activator of local microorganism, (4) technology of pest and disease control on environmentally friendly vegetable crops using botanical insecticides and microbial antagonists; (5) water use efficiency technology with drip irrigation; (6) good sorting, grading and packing technology, 7) empowerment technology to form groups and institutional strengthening and group management. The results of this activity are: (1) the target group / members The women farmer group has a very high curiosity about new information that can improve their standard of living,(2) technology transfer activities on the use of garden with organic vegetable cultivation received positive response from the members of the Group, (3) This devotional activity can encourage the pattern of mind and pattern of member action in organic vegetable cultivation by developing rural resources and sustainable local wisdom, (4) Verikultur cultivation technique with vertical paralon type on a land area of 10 m2 is economically profitable 
KESESUAIAN TIGA BAKTERI ANTAGONIS DENGAN EMPAT JENIS MINYAK ATSIRI Resti Fajarfika; Heru Adi Djatmiko; Darini Sri Utami
Jurnal Ilmiah Media Agrosains Vol 5 No 1 (2019): Edisi Desember
Publisher : Unit Penelitian dan Pengabdian Masyarakat (UP2M) Politeknik Banjarnegara

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Abstract

Penelitian ini bertujuan untuk menguji kesesuaian tiga bakteri antagonis dengan empatjenis minyak atsiri. Pengendalian hayati menggunakan bakteri Bacillus sp. B298,Streptomyces spp. S4, dan Pseudomonas fluorescens dengan memadukan pengendaliannabati (minyak atsiri cengkeh, serai wangi, temulawak dan nilam) pada konsentrasi 0,04; 0,2;1; dan 5%, bakterisida (streptomycin sulfat 20%), air dan pelarut (isopropil alkohol, minyaktanah, dan tween 20) menggunakan metode cakram kertas. Hasil penelitian menunjukkanbahwa tiga bakteri antagonis tidak ada kesesuaian dengan konsentrasi empat jenis minyakatsiri dan bakterisida, tetapi ada kesesuaian terhadap air dan pelarut.
Yield and Quality of Tomatoes On the Giving of Mikotricho and N-P-K Fertilizer Rokhminarsi, Eny; Sri Utami, Darini; Begananda
Jurnal Hortikultura Indonesia (JHI) Vol. 11 No. 3 (2020): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.11.3.192-201

Abstract

Tujuan penelitian adalah mengkaji pemberian pupuk hayati Mikotricho dan pupuk N-P-K terhadap hasil dan kualitas tomat. Penelitian dilaksanakan pada bulan Mei sampai Oktober 2020. Penelitian berupa percobaan faktorial di screenhouse. Rancangan yang digunakan adalah Rancangan Acak Kelompok Lengkap (RAKL) dengan tiga ulangan. Faktor pertama adalah dosis pupuk Mikotricho yaitu 10 g, 30 g, dan 50 g tanaman-1. Faktor kedua adalah pengurangan dosis pupuk N-P-K yaitu pengurangan 0%, 25%, 50% dari dosis anjuran dan kontrol (tanpa pupuk Mikotricho dan tanpa pupuk N-P-K). Data dianalisis menggunakan analisis ragam dan uji lanjut BNT 5%. Hasil penelitian menunjukkan bahwa aplikasi pupuk mikotricho pada budidaya tomat dapat meningkatkan volume buah dan hasil buah tanaman-1 pada dosis 30 g tanaman-1 dan tanpa pegurangan pupuk N-P-K yang berupa urea, SP-36 dan KCl dari dosis anjuran. Pada jumlah buah tanaman-1, aplikasi pupuk mikotricho dosis 30 g tanaman-1 dapat meningkat dengan pengurangan dosis N-P-K sebanyak 25%. Aplikasi pupuk mikotricho pada dosis 30 g tanaman-1 juga dapat meningkatkan kandungan vitamin C dan mengurangi pupuk N-P-K hingga 25%, sedangkan kadar gula dalam buah meningkat pada pemberian pupuk mikotricho dosis 10 g tanaman-1. Kata kunci: mikoriza, produksi, sayuran, trichoderma
Pemanfaatan Mikoriza-Trichoderma dan Pupuk Anorganik terhadap Pertumbuhan, Hasil dan Vitamin C Kubis Bunga Eny Rokhminarsi; Darini Sri Utami; Wilis Cahyani; Okti Herliana
Jurnal Hortikultura Indonesia (JHI) Vol. 13 No. 3 (2022): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.13.3.140-147

Abstract

Kubis bunga merupakan komoditas sayuran yang potensial karena mengandung vitamin dan mineral. Budidaya kubis bunga perlu dikembangkan ke lahan marjinal seperti Ultisol. Pemanfaatan mikoriza-trichoderma dapat meningkatkan kesuburan ultisol. Tujuan penelitian untuk menentukan dosis mikoriza dan trichoderma dengan pengurangan pupuk anorganik terhadap pertumbuhan, hasil dan kandungan vitamin C kubis bunga di ultisol. Penelitian berupa percobaan pot yang diletakkan di lahan menggunakan rancangan acak kelompok lengkap faktorial yang diulang tiga kali. Faktor pertama adalah kombinasi mikoriza+Trichoderma yaitu 0 g + 0 g (tanpa mikoriza+trichoderma), 10 g + 10 g dan 20 g + 20 g, faktor kedua adalah pengurangan pupuk anorganik (urea, SP36 dan KCl) dari dosis rekomendasi yaitu 0%, 25% dan 50%. Data dianalisis dengan uji F dan DMRT. Hasil penelitian menunjukkan bahwa pemberian 20 g mikoriza + 20 g trichoderma tanaman-1 meningkatkan persentase infeksi mikoriza 56.7% dibandingkan dengan 10 g mikoriza + 10 g Trichoderma tanaman-1. Kombinasi terbaik dengan tanaman tertinggi diperoleh pada tanpa mikoriza-Trichoderma dengan pengurangan 25% pupuk anorganik, sedangkan pada bobot akar kering tertinggi pada 10 g mikoriza + 10 g Trichoderma dengan 50% pupuk anorganik. Kombinasi terbaik dengan hasil (curd) tertinggi diperoleh pada 20 g mikoriza + 20 g Trichoderma dengan pengurangan dosis pupuk anorganik 25%, sedangkan kandungan vitamin C tertinggi pada 20 g mikoriza + 20 g trichoderma dengan pengurangan anorganik sebesar 50%.Kata kunci: budidaya, lahan marjinal, pupuk hayati, sayuran