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All Journal Jurnal Fitopatologi Indonesia Pembangunan Pedesaan Prosiding Jurnal Agro Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Kultivasi Caraka Tani: Journal of Sustainable Agriculture Jurnal SOLMA Agrin : Jurnal Penelitian Pertanian Jurnal Agritechno Jurnal Agro Wiralodra Agro Bali: Agricultural Journal Akta Agrosia

Ruth Feti Rahayuniati

Fakultas Pertanian Universitas Jenderal Soedirman Purwokerto

Author-ID : 355167

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Control & Systems Engineering Education Engineering Environmental Science Medicine & Pharmacology Other

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PEMANFAATAN Bacillus sp. DAN Pseudomonas fluorescens UNTUK MENGENDALIKAN PENYAKIT LAYU TOMAT AKIBAT SINERGI R. solanacaerum DAN Meloidogyne sp. Mugiastuti, Endang; Rahayuniati, Ruth Feti; Sulistyanto, Prasmaji
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

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Penelitian ini bertujuan untuk mengetahui kemampuan bakteri Bacillus sp. dan P. fluorescens dalam mengendalikan penyakit layu akibat sinergi M. incoqnita dan R.solanacearum pada tanaman tomat di rumah kaca. Pseudomonas fluorescens P8 merupakan bakteri antagonis yang terbaik untuk mengendalikan penyakit layu tomat dengan menekan masa inkubasi 95,39%, intensitas penyakit 69,95%, dan tingkat kerusakan akar karena nematode, serta untuk meningkatkan pertumbuhan tanaman dengan meningkatkan bobot segar tanaman 52,80 %, bobot akar tanaman 47,48%, dan jumlah buah 58,86%.

PENGENDALIAN PENYAKIT LAYU FUSARIUM TOMAT: APLIKASI ABU BAHAN ORGANIK DAN JAMUR ANTAGONIS CONTROL OF TOMATO FUSARIAL WILT: APPLICATION OF ORGANIC ASH AND ANTAGONISTIC FUNGI Rahayuniati, Ruth Feti; Mugiastuti, Endang
Pembangunan Pedesaan Vol 9, No 1 (2009)
Publisher : Pembangunan Pedesaan

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The aims of this research were to determine the effect of organic ash and antagonistic fungi on controlling tomato Fusarium wilt. Factorial Completely Randomized Design has been used in hisresearch. There were paddy husk and legume ash as organic ash factor, and T. harzianum and Gvirensasantagonistic fungi factor. The result showed that combination of antagonistic fungi (Tharzianum and G. virens) and organic ash (paddy husk and legume ash) could suppress the disease growth, incubation period, disease severity, and disease rate. The effectiveness of the combination were about 36.79-43.30%.

APPLICATION OF RAW SECONDARY METABOLITES FROM TWO ISOLATES OF Trichoderma harzianum AGAINST ANTHRACNOSE ON RED CHILI PEPPER IN THE FIELD Soesanto, Loekas; Mugiastuti, Endang; Suyanto, Agus; Rahayuniati, Ruth Feti
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 20 No. 1 (2020): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j.hptt.12019-27

Anthracnose on red chili pepper is one of the highly damaging diseases that difficult to control bypesticides. This study aimed to determine the effect of raw secondary metabolites isolated from two isolates of Trichodermaharzianum towards anthracnose as well as the growth and yield of red chili pepper plants in the field. The research used arandomized block design with five treatments and seven replicates. The treatments tested were control, raw secondarymetabolites of T. harzianum T10 and T213, and their combination. The observed variables were incubation period, diseaseintensity, the late population density of T. harzianum, plant height, root length, fresh and dry weight of the plant and root,flowering time, number of fruits per plant, fresh weight of fruit, and phenolic compound content analysis. The result showedthat the raw secondary metabolites from the combination of the two T. harzianum isolates were the best treatments that couldlengthen the incubation period and decrease the disease intensity as 30.2 and 87.05%, respectively. However, these applicationscould not increase the growth components; while for the yield components, the application could improve the number of fruitsper plant and fresh weight of fruit as 15.33 and 34.53%, respectively.

Aplikasi Formula Cair Pseudomonas fluorescens P60 untuk Menekan Penyakit Virus Cabai Merah Loekas Soesanto; Endang Mugiastuti; Ruth Feti Rahayuniati
Jurnal Fitopatologi Indonesia Vol 9 No 6 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Viral diseases of chilli pepper are difficult to control, therefore the use of Pseudomonas fluorescens P60 should be evaluated. The aims of this research were to determine the influence of liquid formula of P. fluorescens P60 on virod disease and on growth and yield of chili pepper. Randomized block design (RBD) experiment was composed of 7 treatments and 4 replicates, i.e., control, insecticide applicaton, P. fluorescens P60 application by seedling drenching and spraying for 1, 2, 3, 5, and 7 times. The result showed that 5 times application of P. fluorescens P60 by drenching and spraying was able to suppress viral disease and reduce disease intensity by to 73.37%, increasing density level of P. fluorescens P60 to 9.50 x 1011 and increase phenolic compounds (saponin, tannin and glycoside). The same treatment could increase plant height 23.7%, root lenght 6.44%, plant dry weight 66.68%, root dry weight 23.59%, and yield weight 53.16%.

Aplikasi Bacillus sp. untuk mengendalikan penyakit layu fusarium pada tanaman tomat Endang Mugiastuti; Abdul Manan; Ruth Feti Rahayuniati; Loekas Soesanto
Jurnal Agro Vol 6, No 2 (2019)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/5397

Fusarium oxysporum merupakan penyebab penyakit layu fusarium yang dapat menurunkan produksi tomat. Pengendalian hayati dengan menggunakan bakteri antagonis seperti Bacillus sp. merupakan alternatif pengendalian yang potensial dan ramah lingkungan. Penelitian bertujuan untuk mengetahui kemampuan Bacillus sp. dalam mengendalikan penyakit layu fusarium pada tanaman tomat di lapangan. Percobaan menggunakan rancangan acak kelompok dengan 5 perlakuan dan 5 ulangan, meliputi: kontrol, Bacillus sp. B42, Bacillus sp. B64, gabungan Bacillus sp. B42 dan B64, serta fungisida. Hasil pengujian menunjukkan bahwa Bacillus sp. B.64 merupakan bakteri antagonis terbaik dalam menekan intensitas penyakit layu fusarium, karena dapat menunda masa inkubasi 15,76%, menekan intensitas penyakit 38,77%, meningkatkan kandungan fenol tanaman (tanin, saponin dan glikosida), serta meningkatkan pertumbuhan dan hasil tanaman dengan meningkatkan bobot kering tajuk 32,05%, bobot kering akar 15,23%, dan bobot buah per tanaman 46,48%.ABSTRACTFusarium oxysporum is the causal agent of fusarium wilt disease which decreases the production of tomatoes. Biological control managenement using bacterial antagonists is a potential alternative to prevent the infection of the disease. The aim of this research was to determine the ability of Bacillus sp. to control tomato fusarium wilt in the field. Randomized block design (RBD) experiment was used consisting of 5 treatments and 5 replications i.e. control, Bacillus sp. B42, Bacillus sp. B64, combination of Bacillus sp B42 + B64, and fungicide. The results showed that Bacillus sp. B64 was the best bacterial antagonist agent to control tomato wilt disease by delaying incubation period (15.76%), decreasing disease intensity (38.77%), increasing phenol compounds (tannin, saponin, glycosides) and improving plant growth and yield. Furthermore, the results showed the increasement of shoot dry weight to 32.05%, root dry weight to 15.23%, and yield to 46.48% as well.

Pemanfaatan Beberapa Kaldu Hewan sebagai Bahan Formula Cair Pseudomonas fluorescens P60 untuk Mengendalikan Sclerotium rolfsii pada Tanaman Mentimun Loekas Soesanto; Endang Mugiastuti; Ruth Feti Rahayuniati
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 1 (2011)
Publisher : Universitas Gadjah Mada

A research aiming at knowing the potency of several animal broths as organic liquid formula of Pseudomonas fluorescens P60, soaking period of Sclerotium rolfsii sclerotia, and its application method on cucumber stem-end rot was done. Completely randomized design and randomized block design both arranged by factorial were used for in vitro and in planta tests, respectively. The first factor was six kinds of animal broth, i.e., golden snail, local chicken,broiler chicken, catfish, cow bone, and rat. The second one for in vitro test was the soaking period in the Pseudomonas fluorescens P60 formula, i.e., 0, 1, 10, and 100 hours and for in planta one was application methods, i.e., seed soaking or crop spraying. Result of the research showed that the best animal broth as liquid formula for Pseudomonas fluorescens P60 was golden snail broth indicated by suppression of sclerotial germination up to 97.4% after soaking for 100 hours. The best application method to suppress the disease was spraying method showed by suppressed of sclerotial germination, longer incubation period, and suppressed disease incidence and sclerotial late population of 55.79, 147.35, 66.67, and 59.68%, repectively. Spraying the formula could also increase crop height difference, fresh and dry weight of crop, fresh and dry weight of root, and root length to 146.83, 86.62, 112.5, 87.88, 140, and 159.68%, respectively.

Uji Lapangan Formula Cair Pseudomonas fluorescens P60 terhadap Layu Fusarium pada Tanaman Tomat Loekas Soesanto; Endang Mugiastuti; Ruth Feti Rahayuniati; Abdul Manan
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 2 (2011)
Publisher : Universitas Gadjah Mada

A research aimed at knowing 1) the effect of Pseudomonas fluorescens P60 in liquid formula on Fusarium wilt of tomato, 2) the effect of P. fluorescens P60 in the formula on tomato growth and yield, and 3) P. fluorescens P60 mechanisms on tomata was carried out at tomato field of Selomoyo Village, Kaliangkrik Subdistrict, Magelang Regency at altitude of 826 m above sea level. Randomized block design was used with seven treatments and four replicates. The treatments were control, with P. fluorescens P60 soaked for 15 min and without fungicide, pathogen without P. fluorescens P60 with fungicide (PBG1), pathogen with P. fluorescens P60 without fungicide, pathogen with pouring P. fluorescens P60 1, 3, and 5 times. Result indicated that application of formulated P. fluorescens P60 for 5 times decreased the disease intensity as high as 26.77%, and late population of the pathogen but increased P. fluorescens P60 as high as 4.54×1010 cfu ml-1. P. fluorescens P60 affected growth and yield of tomato. P. Fluorescens P60 induced tomato resistance by increasing qualitatively its phenolic compound content (saponin, tannin, glycoside). Penelitian dengan tujuan untuk mengetahui: 1) pengaruh Pseudomonas fluorescens P60 dalam formula cair terhadap penyakit layu fusarium pada tanaman tomat, 2) pengaruh P. fluorescens P60 dalam formula cair terhadap pertumbuhan dan produksi tanaman tomat, dan 3) mekanisme P. fluorescens P60 pada tanaman tomat dilakukan di lahan Desa Selomoyo, Kecamatan Kaliangkrik, Kabupaten Magelang dengan ketinggian 826 m di atas permukaan laut. Penelitian menggunakan Rancangan Acak Kelompok (RAK), dengan 7 perlakuan dan jumlah ulangan 4 kali, dan setiap unit terdiri atas 8 tanaman. Perlakuan tersebut meliputi kontrol; dengan P. fluorescens P60 rendam 15 menit dan tanpa fungisida; dengan patogen; tanpa P. fluorescens P60; dengan fungisida (PBG1); patogen, tanpa P. fluorescens P60, tanpa fungisida; patogen, dengan penyiraman P. fluorescens P60 1 kali; patogen, dengan penyiraman P. fluorescens P60 3 kali; dan patogen, dengan penyiraman P. fluorescens P60 5 kali. Pemberian P. fluorescens P60 selama 5 kali memberikan pengaruh sangat nyata dalam menekan penyakit layu fusarium yang disebabkan Fusarium oxysporum. Hal ini ditunjukkan pada penurunan intensitas penyakit sebesar 26,77%, rendahnya kepadatan akhir F. oxysporum serta tingginya nilai kepadatan P. fluorescens P60 sebesar 4,54×1010 unit pembentuk spora/ml. Pengaruh pemberian P. fluorescens P60 belum menunjukkan pengaruh nyata pada komponen pertumbuhan dan hasil. P. fluorescens P60 mampu mengimbas ketahanan tanaman tomat dengan meningkatkan kandungan senyawa fenol (saponin, tanin, glikosida).

Pengaruh Pupuk Organik Cair dan Asap Cair dalam Pengendalian Xanthomonas oryzae pv. oryzae dan Pyricularia grisea pada Padi Gogo Galur G136 Iqna Khayatina Rusli; Loekas Soesanto; Ruth Feti Rahayuniati
Jurnal Perlindungan Tanaman Indonesia Vol 20, No 2 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.17690

Increment of upland rice production in Indonesia faces many problems mainly from kresek caused by Xanthomonas oryzae pv. oryzae and blast caused by Pyricularia grisea. The use of Liquid Organic Fertilizer (LOF) and liquid smoke provides an alternative method to control the pathogen. This research aimed at knowing the potency of the leaf fertilizer applied to the soil and the liquid smoke to control the disease in G136 line’s rice and on the crop growth. Randomized Block Design was used with six treatments and replicated four times. The treatments were control without liquid smoke, control with 2% liquid smoke, using 2 ml l-1 LOF Biosena without or with 2% liquid smoke, and using 4 ml l-1 LOF Biosena without or with 2% liquid smoke. The variables observed were incubation period, disease intensity, infection rate, crop height, number of shoots, weight of 1,000 grains and rice production per hectare. The result of the research showed that treatment using the leaf LOF applied to the soil of 4 ml l-1 or 2 ml l-1, and combining with 2% liquid smoke was not able to suppress the kresek and blast development in G136 line. All treatment influenced the number of shoots and the crop height plants, but didn’t influence the weight of 1,000 grains and the rice production per hectare. INTISARIPeningkatan produksi padi gogo di Indonesia menemui banyak kendala di antaranya adalah penyakit kresek yang disebabkan oleh Xanthomonas oryzae pv. oryzae dan penyakit blas yang disebabkan Pyricularia grisea. Penggunaan pupuk organik cair (POC) dan asap cair merupakan salah satu alternatif dalam pengendalian patogen ini. Penelitian bertujuan untuk mengetahui potensi POC daun yang diaplikasikan pada tanah dan asap cair dalam menekan serangan penyebab penyakit pada padi gogo galur G136 serta pengaruhnya terhadap pertumbuhan tanaman. Rancangan yang digunakan adalah Rancangan Acak Kelompok terdiri atas enam perlakuan dan empat ulangan, yaitu kontrol tanpa asap cair, kontrol menggunakan asap cair 2%, 2 ml/l POC Biosena tanpa asap cair dan menggunakan asap cair 2%, 4 ml/l POC Biosena tanpa asap cair dan menggunakan asap cair 2%. Variabel yang diamati adalah masa inkubasi, intensitas penyakit, laju infeksi, tinggi tanaman, jumlah anakan, bobot 1.000 bulir, dan produksi padi per hektar. Hasil penelitian menunjukkan bahwa perlakuan POC daun yang diaplikasikan pada tanah dengan dosis 4ml/l dan 2 ml/l, serta asap cair 2% belum mampu menekan perkembangan penyakit kresek dan blas pada tanaman padi gogo galur G136. Perlakuan berpengaruh terhadap jumlah anakan dan tinggi tanaman tetapi tidak berpengaruh terhadap bobot 1.000 bulir dan produksi padi per hektar.

UJI KESESUAIAN EMPAT ISOLAT TRICHODERMA SPP. DAN DAYA HAMBAT IN VITRO TERHADAP BEBERAPA PATOGEN TANAMAN Loekas Soesanto; Endang Mugiastuti; Ruth Feti Rahayuniati; Ratna Stia Dewi
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 2 (2013): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Compatibility test of four Trichoderma spp. Isolates and in vitro inhibition ability on several plant pathogens. In vitro descriptive compatibility research was carried out to know the compatibility among Trichoderma spp. isolates and their inhibition ability toward several plant pathogens. Four Trichoderma spp. isolates used were ginger, shallot, banana, and pineapple isolates; while the pathogens used were pathogenic fungi (Fusarium, Colletotrichum, Phytophthora, and Sclerotium), bacteria (Ralstonia), and nematode (Meloidogyne dan Globodera). Observation was done toward inhibition zone between Trichoderma spp. isolates, colony radial growth, mycelial dry weight, and nematode mortality. Result of the research indicated that the four Trichoderma spp. isolates were compatible and no growth inhibition was observed. The inhibition ability of all Trichoderma spp. isolates varied and the ginger isolate had the highest inhibition ability and mortality on all fungal species and the nematodes, while on the pathogenic bacteria there was no inhibition.

UJI EMPAT ISOLAT Trichoderma harzianum PADA PENGOMPOSAN KOTORAN SAPI DAN AYAM DAN PENGARUHNYA TERHADAP PERTUMBUHAN MENTIMUN IN PLANTA Muhammad Firdaus Oktafiyanto; Loekas Soesanto; Endang Mugiastuti; Ruth Feti Rahayuniati; Tamad Tamad
Agro Bali : Agricultural Journal Vol 3, No 1 (2020)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (593.796 KB)

Penelitian bertujuan: 1) mengetahui pengaruh empat isolat Trichoderma harzianum dalam pengomposan kotoran (ayam dan sapi), 2) interaksi T. harzianum dan kotoran terhadap pertumbuhan, dan 3) kompos terbaik untuk tanaman mentimun. Rancangan Acak Kelompok digunakan dengan 10 perlakuan dan 3 ulangan. Perlakuan terdiri atas kotoran ayam dan sapi, T. harzianum. T10, T213, T14, dan T16.. Variabel yang diamati pH, C/N rasio, suhu, warna kompos, kepadatan dan kegigasan konidium, komponen pertumbuhan, analis jaringan tanaman, dan serapan N tanaman. T. harzianum T10 dan T14 mampu menurunkan C/N rasio pada kotoran sapi. T. harzianum terbaik adalah T14 pada kotoran sapi yang memiliki nilai tertinggi pada panjang tanaman sebesar 76 cm, panjang akar 22 cm, dan berat basah tajuk 14,96 g. T. harzianum T10 pada kotoran sapi memiiki nilai tertinggi pada berat tanaman segar 17,86 g, bobot basah akar 3,04 g, dan jumlah daun 8,88 helai.DOI: 10.37637/ab.v3i1.424

Co-Authors Abdul Manan Abdul Manan Abdul Manan Agus Suyanto Endang Mugiastuti Endang Mugiastuti Endang Mugiastuti Ersapoetri, Fida Suci Fata, Ghani Husni Iqna Khayatina Rusli Istiqomah, Dina Kurniawan, Ruly Eko Kusuma Leana, Ni Wayan Anik Loekas Soesanto Loekas Soesanto Loekas Soesanto Loekas Soesanto Loekas Soesanto Loekas Soesanto Loekas Soesanto Loekas Soesanto Muhammad Firdaus Oktafiyanto Novianti, Rika Nuraini, Ika Vidi Nurtiati Nurtiati Nurtiati, Nurtiati Prasmaji Sulistyanto Ratna Stia Dewi Rosi Widarawati Ruly Eko Kusuma Kurniawan Sastyawan, Murti Wisnu Ragil SLAMET ROHADI Suroto, Agus Tamad Tamad Totok Agung Dwi Haryanto

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