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All Journal Jurnal Riset Kimia Jurnal Zarah ALCHEMY Jurnal Penelitian Kimia Prosiding Seminar Nasional Pakar Sainstek : Jurnal Sains dan Teknologi Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks
Marniati Salim
Universitas Andalas
Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Energy Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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EMISI DAN ABSORPSI GAS METANA PADA SISTEM PENANAMAN PADI DI AREA TANAH SAWAH Marniati Salim; Hiyal Faizah; I Made Sudiana
Jurnal Riset Kimia Vol. 4 No. 1 (2010): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v4i1.74

Abstract

 To know there are emission and methane absorption, research was conduted by measuring directly gas methane at the rice field and absorption with metanotroph bactery using chromatography gas. From the research, we know that there is methane gas emission at the rice field. Watery land condition, emit methane gas (2,309 mg/L) larger than not watery land (0,059 mg/L). At the same time and paddy age is 2 month, it also emit larger methane gas (1,809 mg/L) than 1 month age paddy (1,758 mg/L) and without paddy (0,697 mg/L), whereas for methane absorption at land sample  T0, T1, T2, T3, shows that with fertilizer given can increase the reduction of injected methane gas. Keywords: Absorption, Metanotroph bacteria, Methane gas emission   
PRODUKSI BIOETANOL DARI SAMPAH DEDAUNAN SEKITAR KAMPUS UNAND DENGAN METODE SSF (SIMULTANEOUS SACHARIFICATION FERMENTATION) Marniati Salim; Elida Mardiah; Yollanda Atmelwidia
Jurnal Riset Kimia Vol. 7 No. 2 (2014): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v7i2.173

Abstract

Vol 7 No 2ABSTRACTIn this research, cellulose from waste leaves are conversed into ethanol by SSF technologyusing cellulase from Trichoderma viride strain T1 sk. In saccharification process, cellulasebreaks cellulase polymer into glucose. Simultaneously, the formed glucose is conversed intoethanol by invertase produced by Saccharomyces cerevisiaewhich is grew on YPD medium.Waste leaves are pretreatmented using basic solutions : basic NaOH 1 %, NH4OH 8 %, NaOH 1% + NH4OH 4 % and NaOH 1 % + NH4OH 8 % with ratio of solid mather (sample) : liquid(basic solution) 1:10 (w/v) with volume of basic solution 100 mL. Immersion time is variatedfor 24, 48 and 72 hours on 500C. The result of research shows that the use of NaOH 1% +NH4OH 4% gives the highest glucose concentration 933,75 μg/mL with immersion time for 72hours on 500C. After being pretreatmented, sample of 0,4 g waste leaves produce the highestglucose concentration. Measurement by GC/MS shows ethanol concentration 62,41% onfermentation time 96 hours with volume of ethanol 2,45 mL for 0,4 g sample.Keywords : Waste leaves, Pretreatment, Trichoderma viride strain T1 sk, SSF, Bioethanol
MEMPELAJARI KONDISI OPTIMUM PEMBUATAN BIOETANOL MELALUI HIDROLISIS ENZIMATIS MENGGUNAKAN Sacharomycess cereviceae DARI PATI SAGU DAN UMBI TALAS Marniati Salim; Elida Mardiah; Yosi Opthymal; Febby Febrizal
Jurnal Riset Kimia Vol. 5 No. 1 (2011): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v5i1.174

Abstract

Vol 5 No 1ABSTRACT Sago (Metroxylon sp) and umbi talas (Colocasia gigantea Hook F) are natural resources that are found in plantyfull Indonesian. However, these potencies are not yet processed to give an optimum value added product. Sago and taro are rich of starch, that could be fermented to form bioethanol for reneweable energy. The ethanol were produced by fermenting the hydrolized starch of sago and taro with yeast. The starch of sago and taro were hydrolized enzymaticly by α-amylase and glucoamylase. Sago and taro (15 g each) were grinded and hydrolized by α-amylase and then by glucoamylase at the variation of volume of 4, 5, 6, 7, and 8 mL for 1, 2, 3, 4, and 5 hours. The glucose produced was measured by Somogy-Nelson methods. The product of sago hydrolysis were optimum with 6 mL of α-amylase and 6 mL glucoamylase for 2 hours to give 59.11 g/L of reducing sugar. The product of taro with 6 mL α-amylase and 7 mL glucoamylase for 4 hours which gave 64.22 g/L of reducing sugar. The product of ethanol were analyzed by Gas Cromatography (GC). The maximum bioethanol production obtained optimum after 4 days fermentation of hydrolized sago starch and after 5 days fermentation of hydrolized taro starch which were 3.742% and 4.0123%. Key words: Bioethanol, sago starch, taro, enzymatic hydrolysed, α-amylase and glucoamylase
PENGARUH HIDROLISIS ENZIMATIS TERHADAP PRODUKSI BIOETANOL DARI UMBI TALAS (Colocasia gigantea Hook F) Marniati Salim; Elida Mardiah; Febby Febrizal
Jurnal Riset Kimia Vol. 5 No. 2 (2012): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v5i2.214

Abstract

Umbi talas (Colocasia gigantea Hook F) are rich of starch (77.9%), that could be fermentated to form bioethanol for reneweable energy. The ethanol were produced by fermenting the hydrolised enzymaticly by α-amylase and glucoamylase at the variation 4, 5, 6, 7, and 8 mL for 1, 2, 3, 4, and 5 hours. The glucose produced was measured by Somogy-Nelson methode. The product of hydrolysed were optimum with 6 mL α-amylase and 7 mL glucoamylase for 4 hours wich gave 64,222 g/L of reducing sugar. The product of ethanol were analyzed by Gas Chromatography (GC). The maximum bioethanol production obtained after 5 days fermentation was 4.0123%.
Pemurnian Fikosianin dari Spirulina platensis dengan Metode Liquid Biphasic Flotation (LBF) dan Penentuan Aktivitas Antioksidannya Cori Yolanda Elise; Syafrizayanti Syafrizayanti; Marniati Salim
Jurnal Riset Kimia Vol. 12 No. 2 (2021): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v12i2.412

Abstract

Phycocyanin from Spirulina platensis (S. Platensis) is a pigment-complex protein belong to the light-harvesting phycobiliprotein family. The pigments have high economic value as a natural blue dye as well as the source of antioxidants and anticancer. Production of pure natural phycocyanin remains in high demand. Therefore, this study aimed to obtain phycocyanin with high purity values using modified liquid biphasic flotation (LBF) system and tested for DPPH (1,1-diphenyl-2-pycrilhydrazil) radical scavenging activity. This study produced high purity phycocyanin with purification fold 3.041 ± 0.04 and recovery yields approximately about 70.881%. Purified phycocyanin showed scavenging activity with IC50 of   338.585 mg/mL. Thus, the LBF system yielded high purity phycocyanin pigments.
PEMBERDAYAAN PEREMPUAN MELALUI TEKNOLOGI QUILLING PAPER SEBAGAI USAHA ALTERNATIF BERBASIS LINGKUNGAN DI DESA MARUNGGI KOTA PARIAMAN Olyy Norita Tetra; Bustanul Arifin; Marniati Salim; Admin Alif; Zulhadjri Zulhadjri
Jurnal Pengabdian Warta Andalas Vol 25 No 1 (2018): Warta Pengabdian Andalas
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Empowering women through the improvement of quilling paper handicraft skill withpaper waste material in Desa Marunggi, Kota Pariaman which is the first winner of WestSumatera Dasawisma but this has not been the activity of used paper mill into handicraftproduct with high selling price. The activity begins with environmental sanitation and healthwaste disposal training, advanced waste paper treatment training with quilling papertechnology with modification process that is expensive expensive quilling paper paper withwaste paper. Simple processing and combined with paper waste around the high crafts, artisticand unique products, such as wall decoration, souvenir, multipurpose box, miniature, jar, bag,tissue box, doll and others. The training activities were attended by ± 40 participants attendedby women in PKK and heads of dasawisma groups. This activity is more interesting because itis held handicraft with handicraft goods from hand made. Through this dedication activity, itis hoped that the cooperation of universities and society so that can increase women's resourcesthrough home industry, increase knowledge, skills and is an improvement program of womenparticipation in development.
Upaya Penanggulangan Pandemi Covid-19 di Panti Asuhan Al-Falah, Padang Refinel Refinel; Emriadi Emriadi; Safni Safni; Mai Efdi; Syukri Syukri; Suryati Suryati; Marniati Salim; Imelda Imelda; Ibnu Irawan; Putri Arwanda
Warta Pengabdian Andalas Vol 27 No 2 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.27.2.113-118.2020

Abstract

The Islamic Boarding School and Orphanage Al-Falah Padang are located on Bypass Km 16, behind the West Sumatra TVRI office. Al-Falah Boarding School has 367 students, 265 of them are foster children in the Orphanage. Inadequate construction and classrooms, the students and foster children of the Islamic Boarding School and the Al-Falah Orphanage studied and lived their daily lives. The occurrence of the Covid-19 pandemic certainly affected the daily activities and learning activities of the orphanage children. Especially, the fulfillment of their basic needs. Moreover, the main problem of Covid-19 is not only about the effects by the virus on sufferers but also about its rapid transmission. Therefore, to help the crisis due to Covid-19, several lecturers and students from the Faculty of Mathematics and Natural Sciences Universitas Andalas (Unand) provided staple foods, money, masks, hand sanitizers, and disinfectants for residents of the Al-Falah Padang orphanage. It is hoped that can help the residents of the Al-Falah orphanage who certainly feel the impact of the Covid-19 pandemic.
UJI SENYAWA ANTIMIKROBA DARI ASAM LEMAK DAN FATTY ACID METHYL ESTER (FAME) MIKROALGA Nannochloropsis oculata Zulkarnain Chaidir; Sari Rahmi; Marniati Salim
Prosiding Seminar Nasional Pakar Prosiding Seminar Nasional Pakar 2019 Buku I
Publisher : Lembaga Penelitian Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/pakar.v0i0.4144

Abstract

Microalgae contains many bioactive molecules including lipid that can be useful for development of antimicrobial compounds. The aim of this research is to test antimicrobial compounds from fatty acid and fatty acid methyl ester (FAME)from microalgae Nannochloropsis oculata. Lipid from microalgae was extracted using hexane and transesterfied using methanol dan sulfuric acid p.a as catalist. Antimicrobial activities were tested using disc diffusion methode against Staphylococcus aureus, Escherichia coli, and yeast Candida albicans, at varying concentrations are 100, 200, and 300 mg/L. The result shown that fatty acids and FAME from microalgae Nannochloropsis oculata have antibacterial activity and do not have anti-candidal activity against Candida albicans. Antibacterial activity of fatty acids is higher than antibacterial activity of FAME on both bacteria. The result shown that fatty acids and FAME contained in lipid of microalgae Nannochloropsis oculata are palmitic acid, stearic acid, methyl lauric, and methyl stearic.
PENAMBAHAN PUPUK KOMERSIAL PADA MEDIUM BBM UNTUK PERTUMBUHAN SPIRULINA PLANTENSIS DAN UJI AKTIVITAS ANTIOKSIDAN Elida Mardiah; Nur Fadillah Pulungan; Marniati Salim
Prosiding Seminar Nasional Pakar Prosiding Seminar Nasional Pakar 2019 Buku I
Publisher : Lembaga Penelitian Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/pakar.v0i0.4153

Abstract

The productivity and quality of Spirulina platensis is determined by the medium growth. One nutrient that can be used for growth Spirulina platensis microalgae are commercial fertilizers including Urea, ZA and TSP. The nitrogen contained in Urea and ZA fertilizers and phosphate in TSP fertilizer will used as an additional nutrient into the growth medium. This research aims to optimize the Spirulina platensis growth medium and test antioxidant activity. Spirulina platensis extract is obtained by sonication and maceration with methanol solvent. The antioxidant activity test was carried out withcalculate IC.50 by spectrophotometry by method1, 1-diphenyl-2-picrylhydrazyl (DPPH). The results showed that fertilizervariations ontreatments P1, P2, P3, P4, P5 and P6 provide optimum growthattreatment P5 (40% urea, 10% ZA and 40% TSP). Spirulina platensis growthon BBM, P5 and BBM medium combined with P5highest cell density on different days of growth. highest is availableon medium C (50% BBM + P5 50%) on day 11. Antioxidant activity inmedium C69,103m g / l, antioxidant activities are classified strong.
Simulasi Inhibisi Aktivitas Enzim α-Amilase dan α-Glukosidase oleh Senyawa Bioaktif Mikroalga Spirulina platensis Syafrizayanti Syafrizayanti; Adetya Putri; Marniati Salim; Arif Juliari Kusnanda
ALCHEMY Jurnal Penelitian Kimia Vol 19, No 2 (2023): September
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.19.2.73120.223-233

Abstract

Diabetes Melitus (DM) Tipe 2 merupakan penyakit dengan gejala hiperglikemia dengan jumlah kasus yang meningkat setiap harinya pada populasi dunia. Enzim yang berperan terkait DM Tipe 2 yaitu α-amilase dan α-glukosidase yang bertindak dalam menghidrolisis pati pada pankreas. Target terapi dalam pengobatan DM Tipe 2 yaitu penurunan reabsorpsi glukosa di usus dengan melakukan penghambatan enzim α-amilase dan α-glukosidase. Tujuan penelitian ini adalah untuk mencari senyawa bioaktif yang terkandung dalam ekstrak Spirulina platensis yang berpotensi menjadi inhibitor α-amilase dan α-glukosidase sebagai antidiabetes secara in silico. Metode yang digunakan berupa skrining senyawa aktif dari literatur, farmakokinetika dengan prediksi ADMET, Lipinski’s Rule of Five dan simulasi penambatan molekul dengan program MOE. Berdasarkan hasil skrining dan simulasi penambatan molekul menunjukkan bahwa senyawa 4-amino-benzoat dapat berikatan dengan enzim α-amilase dengan afinitas pengikatan sebesar -4,16 kcal.mol-1 dengan situs pengikatan dengan asam amino His 299 dan Asp 195, dan berikatan dengan enzim α-glukosidase dengan afinitas pengikatan sebesar -4,08 kcal.mol-1 dengan situs pengikatan dengan asam amino Asn 58 dan Arg 17. Hasil ini menunjukkan senyawa 4-amino-benzoat dari mikroalga Spirulina platensis dapat dijadikan sebagai kandidat bahan alami dalam pengobatan antidiabetes.Inhibition Activity Simulation of α-Amylase and α-Glucosidase Enzyme by Bioactive Compounds of Microalgae Spirulina platensis. Diabetes Mellitus (DM) Type 2 is a disease with symptoms of hyperglycemia, in which its case number is increasing daily in the world population. Enzymes playing a role related to Type 2 DM, namely α-amylase and α-glucosidase, act in hydrolyzing starch in the pancreas. The therapeutic target in treating Type 2 DM is to decrease glucose reabsorption in the intestine by inhibiting the enzymes α-amylase and α-glucosidase. This study aimed to find bioactive compounds in Spirulina platensis extracts that can become α-amylase and α-glucosidase inhibitors as in silico antidiabetics. The method used was screening active compounds from the literature, pharmacokinetics with ADMET predictions, Lipinski’s Rule of Five, and molecular docking simulations with the MOE program. Based on the screening results and molecular docking simulations, it was shown that the 4-amino-benzoic compound could bind to the α-amylase enzyme with a binding affinity of -4.16 kcal.mol-1 with a binding site with the amino acids His 299 and Asp 195, and bind with the α-glucosidase enzyme with a binding affinity of -4.08 kcal.mol-1 with a binding site with the amino acids Asn 58 and Arg 17. These results show that the 4-amino-benzoic compound from Spirulina platensis microalgae can be a candidate for natural ingredients in antidiabetic treatment.
Co-Authors Abdi Dharma Adetya Putri Admin Alif Arif Juliari Kusnanda Cori Yolanda Elise Elida Mardiah Elida Mardiah Elise, Cori Yolanda Emriadi - Faizah, Hiyal Febby Febrizal Febby Febrizal Febby Febrizal Febrizal, Febby Hiyal Faizah I Made Sudiana Ibnu Irawan Imelda Imelda Mai Efdi Nur Fadillah Pulungan Oktoriza, Ghifarizka Olyy Norita Tetra Opthymal, Yosi Putri Arwanda Refinel - Safni Safni Sari Rahmi Sudiana, I Made Sugiharta, Arwan Suryati Suryati Syafrizayanti, Syafrizayanti Syafrizayanti, Syafrizayanti Syukri Syukri Varitha, Afny Yollanda Atmelwidia Yosi Opthymal Zulhadjri Zulhadjri Zulkarnain Chaidir