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Effect of quadrat shapes on measurement of tree density and basal area: a case study on Scots Pine (Pinus silvestris L.) Robiansyah, Iyan
Buletin Kebun Raya Vol 14, No 2 (2011): Buletin Kebun Raya Vol. 14 (2) Juli 2011
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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While quadrat shape of 10 x 10m has been commonly used in tree studies, the efficiency of this shape compared to the other shapes of size 100 m2 has not been tested. In this study, effect of various shapes of 100 m2 quadrat on measurement of Scots Pine (Pinus silvestris L.) density and basal area would be assessed. The aim was to assess the effect of quadrat shapes of 10 x 10m, 5 x 20m and 2 x 50m on the estimation of density and basal area of the pine in two woodlands in Norwich and to find the most efficient shape, statistically and with respect to sampling time. The results from all quadrat shapes similarly showed that the density and basal area of the Pines between two woodlands were significantly different. In site with lower pine density, the density and basal area measurement were not affected by variety of quadrat shapes. In site where the density of the Pine was higher, however, mean density and basal area measurement using 2x50m quadrat gave higher value than of the other two shapes. In term of efficiency, all shapes were not significantly different in statistical precision and sampling time. Shape of 5x20m, however, was more preferable as it required fewer samples before the mean of density and basal area always falls inside the 95% confidence interval of whole samples mean.

CHARACTERIZATION OF A NEW BIOTYPE Moringa OF SAUDI ARABIA USING RAPD AND ISSR MARKERS Robiansyah, Iyan; Ramadan, Ahmed; Al-kordy, Magdy A.; Ghushash, Ahmad S.; Hajar, Abdulrahaman S.
Buletin Kebun Raya Vol 18, No 2 (2015): Buletin Kebun Raya Vol. 18 (2) July 2015
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Moringa peregrina and M. oleifera are the only Moringa species found in Saudi Arabia. Both species are drought resistant and have very high nutritional and medicinal properties. Detection of genetic diversity is of great value for the improvement of nutritional and medicinal value of these plants. The aim of the present study was to characterize a new biotype Moringa observed in Al Bahah Region, Saudi Arabia. We used 11 RAPD and 15 ISSR primers to characterize and compare the new biotype with M. peregrina and M. oleifera. Level of polymorphism generated by each marker was calculated. We also calculate Nei and Liâs coefficient to measure the genetic distance between the studied species. Level of polymorphism generated by RAPD and ISSR was 46% and 57%, respectively. RAPD and ISSR primers revealed that the new biotype shared 55 amplicons (45.08%) with both M. peregrina and M. oleifera, 28 amplicons with M. peregrina (22.95%), 21 amplicons (17.21%) with M. oleifera, and displayed 18 unshared amplicons (14.75%). Based on RAPD data, genetic distance between M. oleifera and M. peregrina was 0.32, whereas genetic distance between the new biotype and M. oleifera and M. peregrina was 0.21 and 0.29, respectively. For ISSR data, genetic distance between M. oleifera and M. peregrina was 0.5, whereas genetic distance between the new biotype and M. oleifera and M. peregrina was 0.36 and 0.34, respectively. Based on these results we suggested that the new biotype is a hybrid crossbred between M. peregrina and M. oleifera.

Effect of quadrat shapes on measurement of tree density and basal area: a case study on Scots Pine (Pinus silvestris L.) Robiansyah, Iyan
Buletin Kebun Raya Vol 14, No 2 (2011): Buletin Kebun Raya Vol. 14 (2) July 2011
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar

While quadrat shape of 10 x 10m has been commonly used in tree studies, the efficiency of this shape compared to the other shapes of size 100 m2 has not been tested. In this study, effect of various shapes of 100 m2 quadrat on measurement of Scots Pine (Pinus silvestris L.) density and basal area would be assessed. The aim was to assess the effect of quadrat shapes of 10 x 10m, 5 x 20m and 2 x 50m on the estimation of density and basal area of the pine in two woodlands in Norwich and to find the most efficient shape, statistically and with respect to sampling time. The results from all quadrat shapes similarly showed that the density and basal area of the Pines between two woodlands were significantly different. In site with lower pine density, the density and basal area measurement were not affected by variety of quadrat shapes. In site where the density of the Pine was higher, however, mean density and basal area measurement using 2x50m quadrat gave higher value than of the other two shapes. In term of efficiency, all shapes were not significantly different in statistical precision and sampling time. Shape of 5x20m, however, was more preferable as it required fewer samples before the mean of density and basal area always falls inside the 95% confidence interval of whole samples mean.

THE USEFULNESS OF ECOLOGICAL NICHE CONCEPTS IN UNDERSTANDING PLANT COMMUNITIES Robiansyah, Iyan
Buletin Kebun Raya Vol 15, No 1 (2012): Buletin Kebun Raya Vol. 15 (1) January 2012
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Plant communities have been attracting many scientists due to their complexity and unclear underlying mechanisms that support it. How large numbers of competing plant species manage to coexist, for instance, is one of the major unresolved questions in plant community ecology. Ecological niche concept is a classical theory which tries to address this question. The development of this concept will be presented in this paper. Recent studies, although few in number and incomplete in many ways, do suggest that plants segregate along various environmental niche axes. Although it is unlikely that niche separation along environmental axes is the only mechanism of coexistence in any large community, the evidence now suggests that ecological niche concept plays a more significant role than has been previously appreciated.

PREDICTING HABITAT DISTRIBUTION OF ENDEMIC AND CRITICALLY ENDANGERED DIPTEROCARPUS LITTORALIS IN NUSAKAMBANGAN, INDONESIA Robiansyah, Iyan
REINWARDTIA Vol 16, No 1 (2017): Vol.16 No.1
Publisher : Research Center for Biology

ROBIANSYAH, I. 2017. Predicting habitat distribution of endemic and critically endangered Dipterocarpus littoralis in Nusakambangan, Indonesia. Reinwardtia 16(1): 11 - 18. - The tree species Dipterocarpus littoralis (Bl.) Kurz. is endemic to Nusakambangan and categorized as critically endangered. In the present study, the habitat suitability of the species in Nusakambangan was predicted using logistic regression analysis and Maxent model. Three topographic variables (elevation, slope, and aspect), distance from river and coastline, and one vegetation index (Normalized Difference Vegetation Index (NDVI)) as well as two water content indexes (Normalized Difference Water Index (NDWI) and Normalized Difference Moisture Index (NDMI)) were used as predictors of the models. Employing initial number of 82 presence and 250 absence data of D. littoralis, both models were able to predict the suitable areas for the species with fairly high success rate. The AUC and Kappa value for logistic regression were 0.77 Â± 0.027 and 0.34 Â± 0.058, respectively, while the respected values for Maxent were 0.91 Â± 0.062 and 0.37 Â± 0.025. Logistic regression analysis identified a total area of 26.13 km2 to be suitable for D. littoralis, while a smaller suitable area (7.85 km2) was predicted by Maxent model. Coastal areas in the west part of the island were predicted by both models as areas with high suitability for D. littoralis. Furthermore, distance from coastline and river, elevation, NDVI, NDWI and NDMI were suggested to be very important for the species ecology and distribution. The results of this study may serve as a basis for population reinforcement and reintroduction programs of D. littoralis and guide for ecosystem management of Nusakambangan Island as a whole.Â

Pengaruh Jalan Terhadap Keragaman Jenis Tumbuhan Bawah dan Habitatnya di Koridor Taman Nasional Gunung Halimun Salak, Jawa Barat Robiansyah, Iyan; Purnomo, Danang W.
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

The road across the forest can cause the diversity exchange in the forest ecosystem. Distribution of the exotic speciescaused by the road are the main factor affecting the extinction of the native plants. The forest corridor in HalimunSalak National Park is separated by the road which is connecting five villages in surroundings. The aims of the researchwere to determine the response of diversity and abundance of the understory plants to the road existence, toobtain the effect of the road to the habitat condition, and to identify the exotic plants and its relation to the road.Vegetations were observed by transect sampling system, 5 transects of 150 m length were placed in forest corridorside along the road. In each transect, there were 12 sampling plots (1m x 1m) placed in distance (from the road): 0,5, 10, 15, 20, 30, 40, 45, 60, 90, 120, and 150 m from the road. The distance of each transect was approximately100 m and each transect were placed in the corridor area having forest buffer about 100 m distance. There were 117plant species including 9 exotic species. Plant community analysis using Squared Euclidean Distance (SED) showedthat road side area to 5 m in distance showed the different composition of the understory plant to inside the forest.Exotic plants and grass dominated in the area close to the road. Canopy cover in the road side to 10 meter to theforest was relatively opened than inside the forest. Plant diversity analysis on both of all species and local speciesusing Canonical Correspondence Analysis (CCA) showed that species diversity of understory plants was notsignificantly affected by the distance from the road. Nevertheless, distance from the road was a main factorinfluencing the exotic species, while distance of 0 m showed the highest exotic plants diversity.Keywords: Corridor, exotic plant, Halimun Salak National Park, road, squared euclidean distance, understory plant

PENGARUH JALAN TERHADAP KERAGAMAN JENIS TUMBUHAN BAWAH DAN HABITATNYA DI KORIDOR TAMAN NASIONAL GUNUNG HALIMUN SALAK, JAWA BARAT Robiansyah, Iyan; Purnomo, Danang W.
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v9i2.165

The road across the forest can cause the diversity exchange in the forest ecosystem. Distribution of the exotic speciescaused by the road are the main factor affecting the extinction of the native plants. The forest corridor in HalimunSalak National Park is separated by the road which is connecting five villages in surroundings. The aims of the researchwere to determine the response of diversity and abundance of the understory plants to the road existence, toobtain the effect of the road to the habitat condition, and to identify the exotic plants and its relation to the road.Vegetations were observed by transect sampling system, 5 transects of 150 m length were placed in forest corridorside along the road. In each transect, there were 12 sampling plots (1m x 1m) placed in distance (from the road): 0,5, 10, 15, 20, 30, 40, 45, 60, 90, 120, and 150 m from the road. The distance of each transect was approximately100 m and each transect were placed in the corridor area having forest buffer about 100 m distance. There were 117plant species including 9 exotic species. Plant community analysis using Squared Euclidean Distance (SED) showedthat road side area to 5 m in distance showed the different composition of the understory plant to inside the forest.Exotic plants and grass dominated in the area close to the road. Canopy cover in the road side to 10 meter to theforest was relatively opened than inside the forest. Plant diversity analysis on both of all species and local speciesusing Canonical Correspondence Analysis (CCA) showed that species diversity of understory plants was notsignificantly affected by the distance from the road. Nevertheless, distance from the road was a main factorinfluencing the exotic species, while distance of 0 m showed the highest exotic plants diversity.Keywords: Corridor, exotic plant, Halimun Salak National Park, road, squared euclidean distance, understory plant

Population Status and Habitat Preferences of Critically Endangered Dipterocarpus littoralis in West Nusakambangan, Indonesia Robiansyah, Iyan; Davy, Anthony John
Makara Journal of Science Vol. 19, No. 4
Publisher : UI Scholars Hub

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The conservation of the endemic tree species Dipterocarpus littoralis (Bl.) Kurz. is hampered by the paucity of information on its population biology and ecology. Consequently, a targeted survey was carried out in the West Nusakambangan Nature Reserve to assess its population size and structure as well as habitat preferences. In total, 676 individuals of D. littoralis were located at 52 locations, with an extent of occurrence of 3.66 km2 and an area of occupancy of 1.71 km2. The population had an inverse-J-shaped distribution of diameter at breast height (DBH), with 63% of individuals in the 0-5 cm class and another 21% in the 5-10 cm class; only 11 (1.6%) mature individuals (DBH≥30) were found. D. littoralis was associated with steep, low, southwest-facing sites and sites that had high litter cover and thickness. Illegal logging and fuel-wood chopping were the main threats to D. littoralis and its habitat. In addition, an invasive shrub, Langkap (Arenga obtusifolia, Arecaceae), was a potential competitor with the seedlings throughout the reserve. In view of its endemism, narrow range and localized distribution, small population, environmental preferences, and the severe threats from anthropogenic activities and invasive species, D. littoralis appears to more than justify its conservation status of Critically Endangered.

The Biology of Invasive Native Plant as an Ex-situ Collection: A Case Study of Epipremnum pinnatum (L.) Engl. (Araceae) in Bogor Botanical Garden, Indonesia zulkarnaen, rizmoon; Martiansyah, Irfan; Damayanti, Frisca; Wardani, Fitri Fatma; Yudaputra, Angga; Robiansyah, Iyan; Hutabarat, Prima Wahyu K.; Harto, Harto
Jurnal Riset Biologi dan Aplikasinya Vol. 6 No. 2 (2024)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v6n2.p60-72

The Bogor Botanical Garden (BBG) is a pivotal center for plant research and conservation, boasting a diverse collection of over 12,000 individual plants spanning various species. However, among the plant collections at BBG, some of them have the potential to be invasive. Therefore, this study aimed to uncover the plant collections at BBG with invasive potential and analyze the distribution of one of the most dominant collections, Epipremnum pinnatum (L.) Engl. The method employed in this research involved a literature review to identify which plant collections have invasive potential and a vegetation analysis on the distribution of E. pinnatum within BBG. Additionally, ANOVA analysis and regression models were employed to explore the relationship between environmental factors and E. pinnatum abundance. Results revealed that BBG hosted 78 potentially invasive plant species, with E. pinnatum comprising 28 individuals. Spatial analysis indicates a clustered distribution of E. pinnatum, influenced by seed dispersal mechanisms, environmental factors, and biotic interactions. Correlation analysis links soil pH, soil moisture, and canopy cover to E. pinnatum distribution. Canopy cover demonstrates a significant positive correlation with E. pinnatum abundance, suggesting its importance in providing favorable microclimates for growth. Regression analysis further supports canopy cover as a predictor of E. pinnatum presence. However, while a strong statistical association was observed, causal relationships require further investigation. This study underscores the complexity of ecological dynamics in BBG and emphasizes the need for comprehensive research to define underlying mechanisms driving plant distributions and interactions.

Optimization of DNA Isolation Dried Leaf Samples of Endangered Plants Dipterocarpus cinereus Ruri Fitriyani; Robiansyah, Iyan; Achyar, Afifatul
Tropical Genetics Vol. 2 No. 1 (2022):
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v2i1.19

Dipterocarpus cinereus (Lagan bras) is an endemic tree of Mursala, a small island in Central Tapanuli, North Sumatra. Lagan bras is a tree that has good quality for shipbuilding or construction materials. This plant has a critical status by the IUCN Red List 2020 so that conservation efforts need to be carried out. Conservation efforts are still limited to the status of conservation, ecology and distribution as well as vegetative propagation while molecular studies have never been carried out. Therefore, it is necessary to know the optimum DNA isolation method because DNA quality is very important as a determinant of the success of molecular analyzes such as PCR. Various techniques can be used to isolate DNA, depending on the type of plant used. Each type of plant contains different secondary metabolite compounds that require an optimum isolation method. This study aims to obtain the optimum DNA isolation method and produce good quality genomic DNA from the dried plant Dipterocarpus cinereus from the LIPI Plant Conservation Research Center and Botanical Gardens collection. This research was conducted at the Genetics and Biotechnology Laboratory, State University of Padang in October 2021. The DNA isolation method used was the modified CTAB method with variations in leaf size, the use of PVP, and grinding with and without liquid nitrogen as well as the Promega and Qiagen isolation kit methods. The results showed that a large quantity of DNA and high quality DNA could be produced in plant samples by modifying DNA isolation using the Cetyl Trimethyl Ammonium Bromide (CTAB) method as well as the addition of polyvinylpolypyrrolidone (PVP) antioxidants and the use of liquid nitrogen with a leaf size of 2x2 cm.

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