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All Journal Tropical Genetics Indonesian Journal of Pharmacology and Therapy
Ruri Fitriyani
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Immunology & microbiology Medicine & Pharmacology

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Optimization of DNA Isolation Dried Leaf Samples of Endangered Plants Dipterocarpus cinereus Ruri Fitriyani; Robiansyah, Iyan; Achyar, Afifatul
Tropical Genetics Vol. 2 No. 1 (2022):
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/tg.v2i1.19

Abstract

Dipterocarpus cinereus (Lagan bras) is an endemic tree of Mursala, a small island in Central Tapanuli, North Sumatra. Lagan bras is a tree that has good quality for shipbuilding or construction materials. This plant has a critical status by the IUCN Red List 2020 so that conservation efforts need to be carried out. Conservation efforts are still limited to the status of conservation, ecology and distribution as well as vegetative propagation while molecular studies have never been carried out. Therefore, it is necessary to know the optimum DNA isolation method because DNA quality is very important as a determinant of the success of molecular analyzes such as PCR. Various techniques can be used to isolate DNA, depending on the type of plant used. Each type of plant contains different secondary metabolite compounds that require an optimum isolation method. This study aims to obtain the optimum DNA isolation method and produce good quality genomic DNA from the dried plant Dipterocarpus cinereus from the LIPI Plant Conservation Research Center and Botanical Gardens collection. This research was conducted at the Genetics and Biotechnology Laboratory, State University of Padang in October 2021. The DNA isolation method used was the modified CTAB method with variations in leaf size, the use of PVP, and grinding with and without liquid nitrogen as well as the Promega and Qiagen isolation kit methods. The results showed that a large quantity of DNA and high quality DNA could be produced in plant samples by modifying DNA isolation using the Cetyl Trimethyl Ammonium Bromide (CTAB) method as well as the addition of polyvinylpolypyrrolidone (PVP) antioxidants and the use of liquid nitrogen with a leaf size of 2x2 cm.
Better understanding of biomolecules as drug target: a literature review Aida Nur Fadhilah; Yohanes Surya Jati; Diyah Novi Sekarini; Laelatul Afifah; Paula Natasha Arincy Shellaginella Vierin; Ruri Fitriyani
Indonesian Journal of Pharmacology and Therapy Vol 6 No 1 (2025)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.13074

Abstract

Drugs are small biological therapeutic agents that play a crucial role in enhancing human health. They have been widely used in the treatment of various diseases, with conventional medicine being one of the most common approaches. However, conventional medicine has several limitations, including non-specific drug targeting, limited efficacy, low stability, poor absorption rates, and degradation due to enzymatic activity and low pH in the digestive system. Efforts to improve drug targeting have led to the development of molecular-based therapies, particularly through the use of biomolecules as drug targets. Drug targeting aims to optimize therapeutic effects by focusing on specific targets while minimizing side effects. This review article explores research findings from the PubMed database, applying specific inclusion and exclusion criteria. It discusses the types of drugs and biomolecular targets, including G protein-coupled receptors (GPCRs), enzymes, ion channel receptors, and DNA, as well as the mechanisms through which drugs interact with these targets in the treatment of various diseases. Additionally, the advantages and disadvantages of these biomolecular targets are examined. In conclusion, the four biomolecules discussed each present unique characteristics as drug targets, highlighting the importance of carefully selecting appropriate molecules based on an understanding of their functions, properties, and interactions with drugs in the body.
Co-Authors Achyar, Afifatul Aida Nur Fadhilah Diyah Novi Sekarini Iyan Robiansyah Laelatul Afifah Paula Natasha Arincy Shellaginella Vierin Yohanes Surya Jati