Titik Purwati Widowati, Titik Purwati
Balai Besar Kulit, Karet dan Plastik - Kementerian Perindustrian

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Pengaruh penggunaan pankreas sapi dan dua jenis bahan penyamak terhadap kualitas fisik kulit skrotum kambing Widowati, Titik Purwati; Suwarastuti, Agustini; Budi P, Amad
Majalah Kulit, Karet, dan Plastik Vol 19, No 1 (2003): Majalah Kulit, Karet, dan Plastik
Publisher : Center for Leather, Rubber, and Plastic Ministry of Industry, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1581.956 KB) | DOI: 10.20543/mkkp.v19i1.246

Abstract

The pancreas of cow was applied as bating agent for wet-salted scrotum skins of goat. The bated skins were then applied with vetetable tanning, chrome-agent and the combination of both agents. The tanned skins were analyzed their tensile strength and elasticity. The results showed that there were significant differencis on those parameters. The tensile strength and elasticity of chrome tanned, vegetable tanned, and the combination of chrome and vegetable tanned-skins were 107,75 kg/cm2 and 100,5%; 56,83 kg/cm2 and 27,83 %;  and 52,28 kg/cm2 and 55,67% respectively. The tensile strength and elasticity of tanned skins indicated a tendency to increase when the concentration of cow pancreas was increased up to 1.5 % and decreased when the concentration reaches 2 % for chrome tanned skins.  Keywords :  cow pancreas, goat scrotum skin, vegetable tanning, chrome tanning.  
Pengaruh penggunaan tepung fleshing dalam pakan ayam pedaging terhadap kenaikan bobot ayam Widowati, Titik Purwati; Susila, R. Jaka; Sutyasmi, Sri
Majalah Kulit, Karet, dan Plastik Vol 18, No 1 (2002): Majalah Barang Kulit, Karet, dan Plastik
Publisher : Center for Leather, Rubber, and Plastic Ministry of Industry, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1667.363 KB) | DOI: 10.20543/mkkp.v18i1.266

Abstract

 The  fleshing waste ratio of leather processing is very high, that was about 70-230 kgs/ton of raw material. Those waste containing high amount of protein. The aim of this experiment was to know the influenceof fleshing powder on the increasing of broiler body weight. This experiment was classified into two steps. The first step was focused on the preparation of fleshing powder. The second step was the application of fleshing powder as protein substitute of broiler feed. The results showed  that the quality of fleshing powder prepared by pressurize steaming at 2 atm for 15 min was better than by unpressurized method. The additional and the final weight of broilers feed by fleshing powder were not significantly difference with the control. Therefore, fleshing powder could be used as protein substitute on the prepraration of broiler feed instead of fish powder.  Key words : fleshing, protein powder,broiler feed, friendly environment. 
Rancang bangun pabrik pengolahan kulit kelinci menjadi kulit kelinci berbulu samak krom skala kecil Harniniek, Harniniek; Widowati, Titik Purwati
Majalah Kulit, Karet, dan Plastik Vol 3, No 7 (1988): Majalah Barang Kulit, Karet, dan Plastik
Publisher : Center for Leather, Rubber, and Plastic Ministry of Industry, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1190.573 KB) | DOI: 10.20543/mkkp.v3i7.287

Abstract

This paper is prepared for a design of a small scale chorme tanning factory, processing fur rabbit leather. The capacity of it will be 200 pieces/day green rabbit skin.  This factory will be profitable in the view of techno – economical calculations are as follows : the total capital is fixed capital + working capital = Rp 49.276.532,00 the total production cost is variable cost for a year + fixed cost for a year = Rp. 41.241.203,76 variable cost for a  year consists of raw material, packing, chemical/supplement material electricity, fixed cost of a year consists of labour salaries, capital interest, maintenance, depreciation and general expences; the manufacturing cost is the total  production divided by production  capacity = Rp. 700,85 / sq. ft; the profit calculation before taxing is Rp. 14.682.636,24, after taxing id Rp. 11.746.108,99; the pay out period calculation consists of the percentage of profit to return the capital (rate of return) before taxing is 29,80 %, 3 years, after taxing 3 years 8 months; the break even point calculations is the break even point is the Rp. 28.768.403,47 the percentage of break even point is 51,44 % and the capacity of break even point is 30.281,29 sq. ft. NBThe price of calculations above at the 1987. 
Karakterisasi protease Bacillus sp. UGM5 Widowati, Titik Purwati; Djojowidagdo, Soemitro; Setiyono, Setiyono; Asmara, Widya
Majalah Kulit, Karet, dan Plastik Vol 14, No 26 (1999): Majalah Barang Kulit, Karet, dan Plastik
Publisher : Center for Leather, Rubber, and Plastic Ministry of Industry, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1359.555 KB) | DOI: 10.20543/mkkp.v14i26.297

Abstract

The objective of this experiment is to indentify the characters of proetease produced by Bacillus sp.UGM5.the protease secreted by Bacillus sp.UGM5 was first isolated,purified and then charactirezed.The crude enzyme has spesific actifity of 1.14 U/mg,however,the spesific activity of purified enzyme was increased by 23.8 times fold and recovery was 33.69%.The Page of nondenatured crude enzymes showes two type of proreases,however ,the SDS-Page of denatured purified enzyme showed four protein-bends with molecular weights of 55.5 kDa,18kDa respecetively.The optimum pH and temperature for the enzyme acrivity are 8.5 and 420C and belongs to serin protease type,with Km 3 X 10-3mM and Vmax 0.0890mM/30 minutes.The activity is not inhibited by Ca+2,Fe+2 and EDTA. INTISARITujuan penelitian ini adalah untuk mengkarakterisasi  protease yang diproduksi  Bacillus sp. UGM5. Protease yang disekresi oleh Bacillus sp.UGM5 pertama-tama diisolasi,dipurifikasi kemudian dikarakterisasi.Enzim kasar mempunyai spesifik 1,14 U/mg.Aktivitas spesifik enzim yang dimurnikan meningkat 23,8 kali dengan recovery 33,69%.Elektroforesis gel poliakrilamid enzim yang dipurifikasi dan didenaturisasi menunjukan ada empat pita protein dengan berat protein masing-masing 55,5 kDa,51 kDa,18 kDa,dan 15,5 kDa .ph dan temperatur optimum aktivitas enzim adalah 8,5 dan 420C,termasuk tipe protease serin,dengan nilai Km 3 X10-3mM dan Vmax 0,0890mM/30menit.Aktivitas enzim tidak dihambat oleh adanya ion Ca2+,Fe+3 dan EDTA.