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THE EFFECT OF POLYVINYL PYROLYDONE (PVP), EXPLANT TYPE, AND EXPLANT PLANTING POSITION ON CALLUS GROWTH OF CIKONENG ST LARGE ORANGE (Citrus maxima (Burm.) Merr) AND SWEET ORANGE (Citrus sinensis (L.) Osbeck) Chaidir, Liberty; Pertiwi, Rahmi
ISTEK Vol. 14 No. 2 (2025)
Publisher : Fakultas Sains dan Teknologi UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/istek.v14i2.1339

Abstract

Cikoneng ST Orange (Citrus maxima (Burm.) Merr) is a germplasm of Sumedang area, which has been scarce for the past few years. The high percentage of browning in Cikoneng ST Orange explants is considered to be the cause of the low percentage of research success. Sweet Orange (Citrus sinensis (L.) Osbeck) is a type of orange that has been widely used as a potential orange explant in tissue culture. This study was conducted to determine the comparison of growth percentage by conducting cross-species research between Cikoneng ST Orange and Sweet Orange. The study was conducted at the Tissue Culture Laboratory of the Agrotechnology Department, Sunan Gunung Djati State Islamic University, Bandung from March to June 2017. The media used was a combination of MS media, 2 mg L-1 2.4 D, and 0.5% PVP. The explants used were leaves and leaf shoots of Cikoneng ST Orange and Sweet Orange. The planting positions of the explants included abaxial and adaxial planting positions. The study was analyzed using a descriptive method. The results showed that the administration of PVP in MS media and 2 mg L-1 2.4 D could induce callus of Sweet Orange at 3 DAI and Cikoneng ST Orange at 5 DAI. The use of leaf shoot explants and adaxial planting position produced the best friable callus up to 28 DAI. This study also found that Sweet Orange had the fastest callus growth time and a low browning percentage compared to Cikoneng ST Orange explants.
Production of kombucha from citrus leaf waste to improve economy and health in Cigugurgirang KBB Kurniasih, Nunung; Rosahdi, Tina Dewi; Kamelia, Lia; Chaidir, Liberty
Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Vol. 11 No. 1 (2026): February 2026
Publisher : University of Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/abdimas.v11i1.16407

Abstract

Cigugurgirang Village, located in Parongpong District, West Bandung Regency, has abundant citrus farming potential. However, the utilization of citrus leaf waste as a commodity with economic and health value remains very limited. In fact, citrus leaves contain bioactive compounds that have the potential to be used as raw materials for functional beverages such as kombucha. Kombucha, in addition to having high nutritional value, is also a fermented drink with strong market potential. This community service program aims to improve public health and economic independence by transforming citrus leaf waste into a functional kombucha product through the active participation of local farmer groups. The method used is a Participatory Action Research (PAR) approach combined with the Asset-Based Community Development (ABCD) approach, involving citrus farmer groups as the main partners. Data were collected through field observations, interviews, and Focus Group Discussions (FGD). The results showed that kombucha fermented for 8 days had the highest nutritional value and was most preferred in terms of taste, aroma, and color. Through a participatory assistance process and the utilization of local assets, the community is able to produce a functional kombucha beverage that not only supports health but also creates new environmentally friendly economic opportunities.