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All Journal Alchemy : Journal of Chemistry
Umi Khamidah, Umi
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Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Materials Science & Nanotechnology

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK METANOL MIKROALGA Chlorella sp. PADA FASE STASIONER HASIL KULTIVASI DALAM MEDIUM EKSTRAK TAUGE (MET) khamidah, Umi; Fasya, Ahmad Ghanaim; Romaidi, Romaidi
ALCHEMY ALCHEMY (Vol.3, No.1
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.478 KB) | DOI: 10.18860/al.v0i0.2893

Abstract

Chlorella sp. is one of microalgae Chlorophyta that containing kinds of important compound such as flavonoid, tanin, phenolic compound, terpenoid, chlorophyll and karotenoid. The purpose of this research are to know antibacterial activity from methanol extract of microalgae Chlorella sp. at stationary phase and to know contains of active compound group in extract of microalgae Chlorella sp. Chlorella sp. was cultivated in Tauge Extract Medium (TEM) 4 % and harvesting of stationary phase. Extraction microalgae Chlorella sp. was performed by maceration with methanol solvent. Methanol extract from stationary phase were tested antibacterial activity with concentration variation 5 %, 10 %, 15 %, 20 %, and 25 % used diffusion method toward E. coli and S. aureus bacterium. Identification of active compound was estimated by reagent tested on qualitative scale include alkaloid, falvonoid, steroid, triterpenoid, and tanin. The result showed that methanol extract of Chlorella sp. in stationary phase in concentration %, 10 %, 15 %, 20 %, and 25 % with inhibition zone as follows 1,0 mm, 3,7 mm, 6,9 mm, 16,5 mm and 12,1 mm toward E. coli bacterium and 2,2 mm, 4,9 mm, 3,0 mm, 7,4 mm, and 13,1 mm toward S. aureus bacterium. The results of the identification of the compound methanol extract of microalgae Chlorella sp.contains a steroid and tanin compound class
UJI AKTIVITAS ANTIBAKTERI EKSTRAK METANOL MIKROALGA Chlorella sp. HASIL KULTIVASI DALAM MEDIUM EKSTRAK TAUGE (MET) PADA TIAP FASE PERTUMBUHAN Fasya, Ahmad Ghanaim; Khamidah, Umi; Amaliyah, Suci; B., Siti Khairul; Romaidi, Romaidi
ALCHEMY ALCHEMY (Vol.2, No.3
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.89 KB) | DOI: 10.18860/al.v0i0.2889

Abstract

Chlorella sp. merupakan salah satu jenis mikroalga Chlorophyta yang mengandung berbagai senyawa penting yang seperti flavonoid, tanin, senyawa fenolik, terpenoid, klorofil dan karotenoid. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak mikroalga Chlorella sp. hasil ekstraksi dengan pelarut metanol pada tiap fase pertumbuhan serta untuk mengetahui kandungan golongan senyawa aktif dalam ekstrak mikroalga Chlorella sp. Chlorella sp. dikultivasi dalam Medium Ekstrak Tauge (MET) 4 %. Pemanenan Chlorella sp. dilakukan pada fase ½ eksponensial, fase ¾ eksponensial, fase awal stasioner, fase stasioner, dan fase akhir stasioner. Ekstraksi mikroalga Chlorella sp. dilakukan dengan metode maserasi menggunakan pelarut metanol. Ekstrak metanol dari masing-masing fase diuji aktivitas antibakteri menggunakan metode difusi cakram terhadap bakteri E. coli dan S. aureus. Identifikasi golongan senyawa aktif dilakukan dengan menggunakan uji reagen secara kualitatif yang meliputi alkaloid, flavonoid, steroid, triterpenoid, dan tanin. Hasil penelitian menunjukkan bahwa ekstrak metanol Chlorella sp. yang memiliki aktivitas antibakteri tertinggi adalah pada fase stasioner dengan zona hambat sebesar 9,9 mm terhadap bakteri E. coli dan 12,0 mm terhadap bakteri S. aureus. Hasil identifikasi golongan senyawa aktif menunjukkan bahwa ekstrak metanol Chlorella sp. mengandung golongan senyawa steroid dan tanin.
Co-Authors A. Ghanaim Fasya Romaidi Romaidi Siti Khairul B., Siti Khairul Suci Amaliyah, Suci