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UJI AKTIVITAS ANTIBAKTERI EKSTRAK METANOL MIKROALGA Chlorella sp. PADA FASE STASIONER HASIL KULTIVASI DALAM MEDIUM EKSTRAK TAUGE (MET) khamidah, Umi; Fasya, Ahmad Ghanaim; Romaidi, Romaidi
ALCHEMY ALCHEMY (Vol.3, No.1
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Chlorella sp. is one of microalgae Chlorophyta that containing kinds of important compound such as flavonoid, tanin, phenolic compound, terpenoid, chlorophyll and karotenoid. The purpose of this research are to know antibacterial activity from methanol extract of microalgae Chlorella sp. at stationary phase and to know contains of active compound group in extract of microalgae Chlorella sp. Chlorella sp. was cultivated in Tauge Extract Medium (TEM) 4 % and harvesting of stationary phase. Extraction microalgae Chlorella sp. was performed by maceration with methanol solvent. Methanol extract from stationary phase were tested antibacterial activity with concentration variation 5 %, 10 %, 15 %, 20 %, and 25 % used diffusion method toward E. coli and S. aureus bacterium. Identification of active compound was estimated by reagent tested on qualitative scale include alkaloid, falvonoid, steroid, triterpenoid, and tanin. The result showed that methanol extract of Chlorella sp. in stationary phase in concentration %, 10 %, 15 %, 20 %, and 25 % with inhibition zone as follows 1,0 mm, 3,7 mm, 6,9 mm, 16,5 mm and 12,1 mm toward E. coli bacterium and 2,2 mm, 4,9 mm, 3,0 mm, 7,4 mm, and 13,1 mm toward S. aureus bacterium. The results of the identification of the compound methanol extract of microalgae Chlorella sp.contains a steroid and tanin compound class

UJI AKTIVITAS ANTIBAKTERI EKSTRAK METANOL MIKROALGA Chlorella sp. HASIL KULTIVASI DALAM MEDIUM EKSTRAK TAUGE (MET) PADA TIAP FASE PERTUMBUHAN Fasya, Ahmad Ghanaim; Khamidah, Umi; Amaliyah, Suci; B., Siti Khairul; Romaidi, Romaidi
ALCHEMY ALCHEMY (Vol.2, No.3
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Chlorella sp. merupakan salah satu jenis mikroalga Chlorophyta yang mengandung berbagai senyawa penting yang seperti flavonoid, tanin, senyawa fenolik, terpenoid, klorofil dan karotenoid. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak mikroalga Chlorella sp. hasil ekstraksi dengan pelarut metanol pada tiap fase pertumbuhan serta untuk mengetahui kandungan golongan senyawa aktif dalam ekstrak mikroalga Chlorella sp. Chlorella sp. dikultivasi dalam Medium Ekstrak Tauge (MET) 4 %. Pemanenan Chlorella sp. dilakukan pada fase Â½ eksponensial, fase Â¾ eksponensial, fase awal stasioner, fase stasioner, dan fase akhir stasioner. Ekstraksi mikroalga Chlorella sp. dilakukan dengan metode maserasi menggunakan pelarut metanol. Ekstrak metanol dari masing-masing fase diuji aktivitas antibakteri menggunakan metode difusi cakram terhadap bakteri E. coli dan S. aureus. Identifikasi golongan senyawa aktif dilakukan dengan menggunakan uji reagen secara kualitatif yang meliputi alkaloid, flavonoid, steroid, triterpenoid, dan tanin. Hasil penelitian menunjukkan bahwa ekstrak metanol Chlorella sp. yang memiliki aktivitas antibakteri tertinggi adalah pada fase stasioner dengan zona hambat sebesar 9,9 mm terhadap bakteri E. coli dan 12,0 mm terhadap bakteri S. aureus. Hasil identifikasi golongan senyawa aktif menunjukkan bahwa ekstrak metanol Chlorella sp. mengandung golongan senyawa steroid dan tanin.

Significance of the Easy-to-use Water Quality Checker for Participative Environmental Monitoring and Experience Based Learning Kikuchi, Akira; Hakim, Luchman; Heryansyah, Arien; Romaidi, Romaidi
Journal of Tropical Life Science Vol 1, No 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Pack Test is a series of products of Kyoritsu Chemical-Check Laboratory Cooperation, Japan. It is easy-to-use, anybody can use at anywhere, low cost, nontoxic, safe, and professional-use, onetime use ion-selective color metric water quality checker. The aim of this research is to assess, then next, discuss the significance of applications of this tool. In this order, NH4-Pack Test was selected as an example for the discussions; although there are more than 60 parameters can be detected by pack test such as, COD, Cl-, NO3-, phosphate, hardness, pH, heavy metals, etc.. As for field survey, Ion chromatography was used to measure ammonium concentration of river water in Jakarta. Then detection range of the NH4-Pack Test was compared to the data, and Indonesian and Malaysian national water quality standard. River water quality of Jakarta was weekly degraded at upstream area, but it was seriously degraded at downstream area (up to 5-10 NH4-N mg/L). As for ammonium concentration, obviously the detection range and step of Pack Test was sufficient to assess the ammonium concentrations of rive water in Jakarta. Of course Pack Test is very simplified tool, environmental water quality standard of ammonia for class I in Indonesia and Class I and II in Malaysia were difficult to evaluate. However, it was obviously applicable to check treated effluent and Class III to V water quality of Malaysian environmental standard. Consequently, it is suggested to adopt a double standard policy of water quality monitoring, such as combination of "easy-to-use simplified" and "conventional-accurate". Because of low cost, and professional-convenient design, implementation of Pack Test will significant to empower on-site water quality monitoring in developing country, participative environmental awareness public programs, experience base environmental learning in schools, and other grass-rooted environmental activities.Keywords: Environmental awareness and education, Pack test, Simplified method, Southeastern Asian county

Mosses Diversity of Tumpak Sewu Waterfall, Lumajang, East Java Al Fajri, M Tajudin; Romaidi, Romaidi
El-Hayah : Jurnal Biologi Vol 6, No 4 (2018): EL-HAYAH (VOL 6, NO 4,March 2018)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v6i4.6412

Mosses or bryophytes, belonging to the lower plants, can grow on higher plants (as epiphyte), stone (epilytic), bark (corticolus), and the surface of the soil. One of the important places in East Java and having a variety of potential mosses is Tumpak Sewu Waterfall. In this place, moss grows well because of the condition of the nature, humidity, and abundant air. This study aimed to identify and calculate the population of mosses at Tumpak Sewu Waterfall, Lumajang, East Java. The research sampling has been performed along hiking trip, riverside and tourist area of Tumpak Sewu Waterfall. Quadratic method with grid lines with a plot size of 1x1 mÂ² on the right and left sides of the river was used to collect and calculate mosses population. Mosses identification was performed using mosses identification key. The population data obtained from this study were analyzed by calculating Frequency, Dominancy and Important Value Index (IVI). This study found 7 species of mosses in which 3 species belong to Family Marcahntiaceae, 2 species belongs to Bryaceae and 1 species belongs to Pottiaceae and Anthocerotaceae, respectively. The highest of IVI value is specimen K2, corresponds to Pohlia flexuosa W.J. Hooker, with 78.178%, and the lowest of IVI value is specimen K6, corresponds to Marchantia sp. with 4.524%. It can be concluded that Tumpak Sewu Waterfall has diversity for mosses that could be useful to conserve Indonesian natural resources especially lower plants.

JENIS-JENIS PAKU EPIFIT DAN TUMBUHAN INANGNYA DI TAHURA RONGGO SOERYO CANGAR Romaidi, Romaidi; Solikha, Maratus; Minarno, Eko Budi
El-Hayah : Jurnal Biologi Vol 3, No 1 (2012): EL-Hayah (Vol 3, No 1, September 2012)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v3i1.2221

A study to know kinds of epiphytic ferns and their host plants has been conducted in Forest Park Ronggo Soeryo Cangar (FPRSC). The method used to inventory kind of ferns and their host plants is cruising method on some defined areas. These areas include (1) the back of the tourist hot Tahura R. Soejo Cangar, (2) the forest area behind the inn of FPRSC and (3) forest areas that are included in the slopes of Mount Arjuna. Once the sample is collected then be identified in the Taxonomy Laboratory, Department of Biology, Faculty of Science and Technology, UIN Maliki Malang. The results showed that there were 12 species of epiphytic ferns type has been found and 19 of its host tree and plant species are generally included in the staging tree.

PENGEMBANGAN BIBIT UNGGUL PORANG (Amarphopallus oncophilus) MELALUI TEKNIK KULTUR IN VITRO UNTUK MENDUKUNG KETAHANAN PANGAN NASIONAL Suheriyanto, Dwi; Romaidi, Romaidi; Resmisari, Ruri Siti
El-Hayah : Jurnal Biologi Vol 3, No 1 (2012): EL-Hayah (Vol 3, No 1, September 2012)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v3i1.2216

Porang (Amarphophallus. oncophyllus) is a type of plant tubers that have the potential and prospects for development in Indonesia. In addition to readily available, plants are also capable of producing carbohydrates and high harvest index. The last few years a very large porang needs. Porang plant propagation is vegetative and generative in which to make the seeds are ready to harvest takes between 4-6 months. This study aims to determine kinds of the appropriate medium for growing plants porang network and determine the concentrationÂ of Â each Â medium, Â so Â we Â get Â a Â superior Â porang Â plants. Â Research Â using factorial completely randomized design. The first factor is a growth regulator BAP concentration: 0 mg / l, 0.5 mg / l, 1.0 mg / l, 1.5 mg / l and 2.0 mg / l, while the second factor is the concentration of IBA 0 mg / l, 0.5 mg / l and 1.0 mg / l, in order to obtain 15 treatments and each treatment made 15 replications. Data were collected for the pengkalusan, contamination callus, callus mortality rate, number of buds, leaf buds height, number of leaves and root. The results showed that the addition of plant growth regulators (hormones) BAP in MS medium effect on the number of shoots, buds and the high number of young leaves. The addition of hormone IBA in MS medium pengkalusan affect the process and the number of roots. The addition of hormone BAP and IBA on MS medium did not significantly affect mortality contamination callus and callus. The interaction between BAP and IBA hormone given on MS medium did not significantly affect the pengkalusan, contamination callus, callus mortality rate, number of buds, leaf buds height, number of leaves and root Â

Antibacterial activity of water and ethanol extract of Allium sativum, Curcuma mangga, and Acorus calamus combination Bayyinatul Muchtaromah; Mujahidin Ahmad; Romaidi Romaidi; Lina Ainun Nazilah; Nayla Alin Naja
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 1 (2018): December 2018
Publisher : The East Java Biological Society

Madura has been known as an Indonesian tribe that usually used many recipes of traditional medicine in their daily life. One of the medicinal herbs to increase female fertility is the herbal "Subur Kandungan", which consists of garlic (Allium sativum), temu mangga (Curcuma mangga), jeringau (Acorus calamus). The purpose of this study was to determine the phytochemical content and antibacterial activity of A. sativum, C. mangga and A. calamus combination in water and ethanol solvent against Streptococcus aureus and Escherichia coli. There were three kinds of combinations with different composition ie first combination/C1 (36:36:28); second combination/C2 (40:30:30); third combination/C3 (35:40:45). Clindamycin was as a positive control. The phytochemical screening detected triterpenoids in both extracts, while alkaloids, flavonoids only in ethanol extracts. The highest inhibitory zone of both extracts to S. aureus and E. coli were discovered in C3 as strong and moderate inhibition. The most effective MIC value of water extract against S. aureus was found on C3 (9.76 x1010) while ethanol extract was obtained by C1 (5.9x108) at concentrations of 0.39% and MBC at 0.78%. The best MIC value of water extract against E. coli was found on C1 (1.08x1010) at a concentration of 25% and MBC at 50%, whereas ethanol extract was got on C3 (9.7x109) at 0.39% & MBC at 0.78%. It could be concluded that “Subur kandungan” herb recipes could be used as antibacterial drugs, which third combination/C3 in the ethanol solvent is the best treatment compare with others.

CORAL REEFS DIVERSITY IN GILI GENTING ISLAND SUMENEP MADURA Fathorrohman Fathorrohman; Romaidi Romaidi; Bayyinatul Muchtaromah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 1 (2013): December 2013
Publisher : The East Java Biological Society

Coral reef ecosystem is the most threatened ecosystem among marine ecosystem in the world due to the combination of anthropogenic and natural disturbances. More research is needed to be monitor ed and assess coral reef ecosystems, which will be used to fin d understanding of the ecological integrity and further improvement of the protection strategy in the future. This re search was aimed to know the diversity of coral reef diversity at Gili Genting Island Sumenep Madura and evaluate the condition of coral reef ecosystem based on cover the percentage. Line intercept method was used to understand coral reef diversity and its condition in the desired observation station. The result revealed that 9 families, 22 genera and 4 5 species of coral have been successfully found, in which Acropora is the most common genus found in in Gili Genting Island . The cover percentage analysis also indicated that the condition of coral reef ecosystem in Gili Genting Island could be classified to very bad category where th e total average of cover percentage is 12.55%. These findings provide the prelim inary information about the condition of coral reef ecosystem in small i sland that might be useful for the future integrated management based on ecological perspective.

Significance of the Easy-to-use Water Quality Checker for Participative Environmental Monitoring and Experience Based Learning Akira Kikuchi; Luchman Hakim; Arien Heryansyah; Romaidi Romaidi
Journal of Tropical Life Science Vol. 1 No. 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Pack Test is a series of products of Kyoritsu Chemical-Check Laboratory Cooperation, Japan. It is easy-to-use, anybody can use at anywhere, low cost, nontoxic, safe, and professional-use, onetime use ion-selective color metric water quality checker. The aim of this research is to assess, then next, discuss the significance of applications of this tool. In this order, NH4-Pack Test was selected as an example for the discussions; although there are more than 60 parameters can be detected by pack test such as, COD, Cl-, NO3-, phosphate, hardness, pH, heavy metals, etc.. As for field survey, Ion chromatography was used to measure ammonium concentration of river water in Jakarta. Then detection range of the NH4-Pack Test was compared to the data, and Indonesian and Malaysian national water quality standard. River water quality of Jakarta was weekly degraded at upstream area, but it was seriously degraded at downstream area (up to 5-10 NH4-N mg/L). As for ammonium concentration, obviously the detection range and step of Pack Test was sufficient to assess the ammonium concentrations of rive water in Jakarta. Of course Pack Test is very simplified tool, environmental water quality standard of ammonia for class I in Indonesia and Class I and II in Malaysia were difficult to evaluate. However, it was obviously applicable to check treated effluent and Class III to V water quality of Malaysian environmental standard. Consequently, it is suggested to adopt a double standard policy of water quality monitoring, such as combination of "easy-to-use simplified" and "conventional-accurate". Because of low cost, and professional-convenient design, implementation of Pack Test will significant to empower on-site water quality monitoring in developing country, participative environmental awareness public programs, experience base environmental learning in schools, and other grass-rooted environmental activities.Keywords: Environmental awareness and education, Pack test, Simplified method, Southeastern Asian county

UPAYA PENINGKATAN EKONOMI DAN PENGURANGAN SAMPAH DOMESTIK MASYARAKAT SUMBERSARI MELALUI PEMANFAATAN ALIRAN SUNGAI UNTUK BUDIDAYA IKAN MUJAIR Romaidi, Romaidi; Prahardika, Bayu Agung; Hasyim, Muhammad Asmuni
JRCE (Journal of Research on Community Engagement) Vol 1, No 2 (2020): Journal of Research on Community Engagement
Publisher : Universitas Islam Negeri Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jrce.v1i2.8839

One of the biggest threats from human activities to rivers is the increasing number of residential areas around or even just above the river flow. This condition is certainly very threatening the sustainability of rivers in the region. One of the biggest threats is that the garbage produced by the community will be directly disposed of in the river flow. One way that can be done to reduce waste disposal in the river is by utilizing river flow, so that many rivers are now increasingly being developed as a vehicle for habitat conservation, because the river is considered important for various aquatic plants, migratory and sedentary fishes, and aquaculture ponds, birds and several types of mammals. In this case, the concept of river management must be based on environmental, ecological, economic and social aspects according to the characteristics of the surrounding community. The purpose of this community service is to utilize river flow for tilapia fish farming. The method used in the service is the PAR by way of lectures and direct practice in aquaculture with karamba by utilizing river flow. From the results of community service including partners showed that some respondents know how to fish with karamba, but do not know that river flow can also be used for fish farming with the karamba system. As well as the results of fish farming practices with the karamba system most of the respondents agreed agree (73%) if the fish culture using the karamba system is used as an alternative business idea related to river flow utilization and 60% of respondents agree the use of river flow for fish cultivation can reduce waste disposal in the river.

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