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All Journal JURNAL TEKNOLOGI LINGKUNGAN Indonesian Journal of Biotechnology Jurnal Ilmu Kefarmasian Indonesia Majalah Farmasetika COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat Makara Journal of Science
Witono Basuki, Witono
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Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences

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Biodegradation of Used Engine Oil by Acinetobacter junii TBC 1.2 Basuki, Witono; Syahputra, Khairul; Suryani, Ayu Tri; Pradipta, Ilham
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

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Abstract

AbstractThe isolates have capability to degrade used engine oil was obtained from soil in the beach contaminatedwith used engine oil. One of the selected isolates TBC 1.2 was identified by its 16s rDNA as Acinetobacterjunii. The microorganism can use hydrocarbons in used engine oil as the sole carbon source and energy, alsoit significantly degraded almost all hydrocarbon compounds in used engine oil. With its ability Acinetobacterjunii TBC 1.2 has a potency to be utilized for bioremediation of soil polluted with used engine oil.Keywords : biodegradation, used engine oil, Acenitobacter junii TBC 1.2
BIODEGRADASI LIMBAH OLI BEKAS OLEH Lycinibacillus sphaericus TCP C 2.1 Basuki, Witono
Jurnal Teknologi Lingkungan Vol. 12 No. 2 (2011)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.804 KB) | DOI: 10.29122/jtl.v12i2.1243

Abstract

Mikroorganism has capability to degrade used engine oil was isolated from soil sample contaminated with used engine oil. One of the selected strain TCP C 2.1 was identified by 16s rDNA as Lycinibacillus sphaericus. The microorganism can use hydrocarbon in used engine oil as the sole carbon source and energy, also it significantly degraded almost all hydrocarbon compounds in used engine oil. With its ability the microorganism has potency to be used as a single microbe for bioremediation of soil polluted by engine oil.
Bioconversion of Glycerol to Docosahexaenoic Acid by Thraustochytrium WB-02 an Indigenous Indonesian Microalga Strain Basuki, Witono
Makara Journal of Science Vol. 23, No. 2
Publisher : UI Scholars Hub

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Glycerol is a by-product of the biodiesel industry, and it can be processed to produce many useful derivatives. This study is aimed at examining the bioconversion of glycerol to docosahexaenoic acid (DHA) using local microalgae. Glycerol to docosahexaenoic acid converting microalgae were obtained from the mangrove area in the coastal sea of Lampung Province. The single colony was then generated by the scratching technique in its isolation and purification process. By using 18S rDNA, a potential strain namely WB-02, was identified as Thraustochytrium sp. Gas chromatog-raphy analysis was performed to identify its product conversion. As a result, Thraustochytrium WB-02 was identified toutilize glycerol as a single carbon source and convert to DHA. A maximum DHA yield of more than 3.4 g/L was ob-tained when the glycerol concentration in the medium was 8%. Thraustochytrium WB-02 was regarded as a potential microalgae resource in producing DHA due to its high level of production.
Biodegradation of Used Synthetic Lubricating Oil by Brevundimonas diminuta AKL 1.6 Basuki, Witono
Makara Journal of Science Vol. 21, No. 3
Publisher : UI Scholars Hub

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Abstract

Illegal disposal of used lubricating oil is a serious hazard to the environment and public health. This study is aimed at examining the biodegradation process of used synthetic lubricant using local microorganism. Oil-degrading microorganism were obtained from sea water contaminated with used lubricating oil. Those that showed some growth activity in Bushnell-Haas media containing used synthetic lubricating oil as a sole carbon source were selected. Its single colony was then generated by performing the dilution and scratch technique in the isolation and purification process. By using 16s rDNA, a potential strain namely AKL 1.6, was identified as Brevundimonas diminuta. Gas chromatography analysis was performed before and after the biodegradation process of the used oil. As a result, B. diminuta AKL 1.6 was found to utilize used synthetic lubricating oil as the sole source of carbon and to degrade most of the hydrocarbon within the oil for 14 days. Thus, this particular microorganism has the potential to be the single microbe for bioremediation of sea water contaminated by lubricating oil.
Formulasi Krim Ekstrak Kulit Buah Lemon (Citrus Limon L.) dan Daun Jambu Biji (Psidii Guajava L.) sebagai Antioksidan Irma, Ade Irma; Indrawati, Teti; Basuki, Witono
Majalah Farmasetika Vol 9, No 4 (2024)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/mfarmasetika.v9i4.54725

Abstract

AbstrakIndonesia merupakan negara tropis yang kaya dengan sumber daya alam yang dapat dimanfaatkan diantaranya sebagai kosmetik untuk antioksidan. Tanaman yang memiliki khasiat sebagai antioksidan antara lain kulit buah lemon dan daun jambu biji. Kandungan metabolit sekunder lemon yaitu flavonoid, alkaloid, limonoid, carotenoid, asam fenolat dan minyak atsiri. Daun jambu biji mengandung asam psidiolat, asam kratogolat, asam ursolat asam oleanolat, asam guajaverin, dan asam ursolat. Tujuan penelitian ini untuk memperoleh sediaan krim yang stabil secara fisika dan kimia serta berkhasiat sebagai antioksidan. Ekstrak kulit buah lemon dan daun jambu biji dibuat secara maserasi kinetik dengan pelarut etanol 70%, kemudian diuapkan dengan evaporator. Sediaan krim dibuat dengan memvariasikan perbandingan jumlah ekstrak kulit buah lemon dan daun jambu biji (1:1, 1:2) untuk formula 1 dan 2. Formula krim terdiri dari ekstrak, asam stearat, setil alkohol, isopropil miristat, trietanolamin, gliserin,propilenglikol, metil paraben, propil paraben, dan akuades. Sediaan krim dikarakterisasi meliputi organoleptik, homogenitas, pH, daya sebar, viskositas dan aktivitas antioksidan. Hasil evaluasi diperoleh warna coklat kekuningan pada ekstrak kulit lemon dan coklat tua pada ekstrak daun jambu biji. Bau dan bentuk kedua ekstrak yaitu berbau khas dan kental. Homogenitas sediaan krim ekstrak kulit lemon dan daun jambu biji yaitu homogen. Sediaan pada ekstrak kulit lemon memiliki pH 4,46 ± 0,0153 pada ekstrak kulit lemon dan 6,7 ± 0,0200 pada ekstrak daun jambu, daya sebar 6,2 – 7,3 cm, viskositas sebesar (754) Ps – (2132) Ps, sifat alir tiksotropik plastik. Aktivitas antioksidan vitamin C, ekstrak lemon, ekstrak daun jambu biji dan krim kombinasi berturut-turut sebesar 3,26 ± 0,0577 ppm, 16,46 ± 0,3595 ppm, 20,86 ± 0,5546 ppm dan 58,58 ± 0,4842 ppm. Berdasarkan data yang diperoleh, dapat disimpulkan bahwa sediaan krim kombinasi ekstrak kulit lemon dan daun jambu biji (1:2) memiliki potensi yang baik untuk digunakan sebagai kosmetik sebagai antioksidan dan memenuhi karakterisasi sediaan krim yang memenuhi persyaratan sehingga dapat digunakan. 
Formulation and test of mosquito repellent lotion from the extract of kecombrang (Etlingera elatior (Jack)) and beluntas (Pluchea indica) leaves extract Taurhesia, Shelly; Endah, Endah; Basuki, Witono; Djamil, Ratna
JURNAL ILMU KEFARMASIAN INDONESIA Vol 22 No 1 (2024): JIFI
Publisher : Faculty of Pharmacy, Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v22i1.1521

Abstract

Dengue fever is an infectious disease which is transmitted by Aedes aegypti mosquitoes and commonly occurs in Indonesia. The use of natural ingredients for mosquito repellent is becoming a trend. Kecombrang (Etlingera elatior (Jack)) is known as a medicinal plant which contains alkaloids, saponins, tannins, phenolics, flavonoids, triterpenoids, steroids, and glycosides. Beluntas (Pluchea indica) contains tannins, alkaloids, flavonoids, phenols, saponins and essential oils. This study aims to develop mosquito repellent lotion using combined leaf extracts of E. elatior and P. indica at different ratios (1:1; 1:2; 2:1). The materials used in this study were E. elatior and P. indica leaves, Aedes aegypti mosquitoes, 96% ethanol, stearic acid, triethanolamine, white oil, methyl paraben, cetyl alcohol, and distilled water. The repellent activity is tested using a standard method of WHO, comparing control group and treatment group, repeating for four times, then the protection level was calculated. The three formulas of lotion fulfilled both physical and chemical requirements and had a repellent activity of above 80%. The formulae of F3, a lotion containing both E. elatior and P. indica leaf extracts of 2:1, gave the highest protection of 88.1%.
Evaluasi Penghambatan Enzim ?-Glukosidase Secara Invitro Dari 5 Ekstrak Spesies Syzygium Serta Pengaruh Perbedaan Konsentrasi Pelarut Ulfa, Dina Amalia; Adnan, Adek Zamrud; Basuki, Witono; Chaidir, Chaidir; Lotulung, Puspa Dewi N; Minarti, Minarti
COMSERVA : Jurnal Penelitian dan Pengabdian Masyarakat Vol. 4 No. 7 (2024): COMSERVA : Jurnal Penelitian dan Pengabdian Masyarakat
Publisher : Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/comserva.v4i7.2710

Abstract

Spesies Syzygium merupakan genus dengan jumlah spesies yang sangat banyak dan keanekaragaman yang tinggi. Genus ini banyak ditemukan di daerah tropis karena kesesuaiannya dengan iklim tropis. Lima spesies Syzygium yaitu daun salam (Syzygium polyanthurn), daun jambu air (Syzygium aqueum), daun jambu biji (Syzygium malaccense), daun jambu biji (Syzygium guazava), dan daun pucuk merah (Syzygium campanulatum Korth.) telah digunakan dalam pengobatan tradisional karena kandungan polifenolnya. Penelitian ini bertujuan untuk menghasilkan ekstrak paling efektif dari beberapa spesies Syzygium sp. dengan perbandingan komposisi etanol-air, sebagai obat herbal antidiabetik melalui penghambatan enzim alfa-glikosidase. Kelima spesies Syzygium diekstraksi menggunakan metode maserasi dengan pelarut 96% dengan rendemen rata-rata 30%. Ekstrak kental lima spesies Syzygium diuji antioksidannya dengan nilai rata-rata IC-50 pada Ekstrak Daun Pucuk Merah tercatat sebesar 14,7. Nilai rata-rata IC-50 Ekstrak Daun Salam tercatat sebesar 8,46, Nilai rata-rata IC-50 Ekstrak Daun Jambu Biji Bol sebesar 9,42, Nilai rata-rata IC-50 Ekstrak Daun Jambu Biji yang dihitung dari ketiga pengukuran tersebut adalah 10,51. Nilai rata-rata IC-50 ekstrak daun jambu biji yang dihitung dari ketiga pengukuran tersebut adalah 9,88. Aktivitas penghambatan enzim a-glukosidase daun jambu biji pekat 96% diuji pada berbagai konsentrasi dan diperoleh hasil yang signifikan. Pada konsentrasi 50 ppm, % penghambatan yang diamati sebesar 97,74 dengan nilai IC-50 sebesar 6,42, yang menunjukkan bahwa kemampuan penghambatan terhadap enzim tersebut sangat tinggi. Ketika konsentrasi diturunkan menjadi 25 ppm, % penghambatan yang diamati adalah 89,35, menunjukkan penurunan potensi, meskipun penghambatan tetap signifikan.
Co-Authors Adek Zamrud Adnan Ayu Tri Suryani, Ayu Tri Chaidir Chaidir Djamil, Ratna endah, endah Ilham Pradipta, Ilham Irma, Ade Irma Khairul Syahputra, Khairul Lotulung, Puspa Dewi N Minarti Minarti Rudiyanto, Aris Rudiyanto, Aris Sri Kadarwati Taurhesia, Shelly Teti Indrawati Ulfa, Dina Amalia