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All Journal JURNAL TEKNOLOGI LINGKUNGAN Indonesian Journal of Biotechnology Jurnal Ilmu Kefarmasian Indonesia Majalah Farmasetika COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat Makara Journal of Science
Witono Basuki, Witono
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Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences

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Journal : Makara Journal of Science

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Bioconversion of Glycerol to Docosahexaenoic Acid by Thraustochytrium WB-02 an Indigenous Indonesian Microalga Strain Basuki, Witono
Makara Journal of Science Vol. 23, No. 2
Publisher : UI Scholars Hub

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Abstract

Glycerol is a by-product of the biodiesel industry, and it can be processed to produce many useful derivatives. This study is aimed at examining the bioconversion of glycerol to docosahexaenoic acid (DHA) using local microalgae. Glycerol to docosahexaenoic acid converting microalgae were obtained from the mangrove area in the coastal sea of Lampung Province. The single colony was then generated by the scratching technique in its isolation and purification process. By using 18S rDNA, a potential strain namely WB-02, was identified as Thraustochytrium sp. Gas chromatog-raphy analysis was performed to identify its product conversion. As a result, Thraustochytrium WB-02 was identified toutilize glycerol as a single carbon source and convert to DHA. A maximum DHA yield of more than 3.4 g/L was ob-tained when the glycerol concentration in the medium was 8%. Thraustochytrium WB-02 was regarded as a potential microalgae resource in producing DHA due to its high level of production.
Biodegradation of Used Synthetic Lubricating Oil by Brevundimonas diminuta AKL 1.6 Basuki, Witono
Makara Journal of Science Vol. 21, No. 3
Publisher : UI Scholars Hub

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Abstract

Illegal disposal of used lubricating oil is a serious hazard to the environment and public health. This study is aimed at examining the biodegradation process of used synthetic lubricant using local microorganism. Oil-degrading microorganism were obtained from sea water contaminated with used lubricating oil. Those that showed some growth activity in Bushnell-Haas media containing used synthetic lubricating oil as a sole carbon source were selected. Its single colony was then generated by performing the dilution and scratch technique in the isolation and purification process. By using 16s rDNA, a potential strain namely AKL 1.6, was identified as Brevundimonas diminuta. Gas chromatography analysis was performed before and after the biodegradation process of the used oil. As a result, B. diminuta AKL 1.6 was found to utilize used synthetic lubricating oil as the sole source of carbon and to degrade most of the hydrocarbon within the oil for 14 days. Thus, this particular microorganism has the potential to be the single microbe for bioremediation of sea water contaminated by lubricating oil.
Co-Authors Adek Zamrud Adnan Ayu Tri Suryani, Ayu Tri Chaidir Chaidir Djamil, Ratna endah, endah Ilham Pradipta, Ilham Irma, Ade Irma Khairul Syahputra, Khairul Lotulung, Puspa Dewi N Minarti Minarti Rudiyanto, Aris Rudiyanto, Aris Sri Kadarwati Taurhesia, Shelly Teti Indrawati Ulfa, Dina Amalia