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Pengaruh Lama Perendaman dan Jenis Minuman Beralkohol Bir dan Tuak terhadap Kekerasan Email Gigi Manusia (In Vitro) Magista, Malida; Nuryanti, Archadian; Wahyudi, Ivan Arie
Majalah Kedokteran Gigi Indonesia Vol 21, No 1 (2014)
Publisher : Majalah Kedokteran Gigi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Erosi gigi merupakan hilangnya lapisan email gigi karena asam. Jenis asam, pH rendah, serta kandungan kalsium, fosfat, dan fluoride pada bir dan tuak diduga merupakan faktor kimiawi penyebab erosi gigi. Tujuan penelitian ini untuk mengetahui pengaruh lama perendaman dan jenis minuman beralkohol bir dan tuak terhadap kekerasan email gigi manusia (in vitro). Penelitian ini menggunakan 14 sampel gigi premolar pertama atas. Setiap gigi dibagi menjadi 2 bagian, bukal dan palatal. kemudian dibagi menjadi 7 kelompok perlakuan, yaitu kelompok (A1) perendaman dalam bir (ringan); (A2) kelompok perendaman dalam bir sedang; kelompok (A3) perendaman dalam bir berat, kelompok (B1): perendaman dalam tuak ringan, (B2): perendaman dalam tuak sedang, (B3): perendaman dalam tuak berat dan kelompok kontrol (C). Uji kekerasan email gigi dilakukan menggunakan Micro Vickers Hardness Tester. Pengujian kekerasan awal email gigi dilakukan sebelum perendaman gigi. Perendaman gigi premolar pertama atas pada bir dan tuak dilakukan selama 10 detik, 50 detik, dan 250 detik perhari dengan penyimpanan subjek penelitian pada saliva buatan. Uji kekerasan akhir email gigi dilakukan setelah perlakuan selama 30 hari. Nilai perubahan kekerasan email gigi merupakan selisih nilai kekerasan akhir dan nilai kekerasan awal email gigi. Sebagai tambahan data, pada bir dan tuak juga diukur kandungan pH, kalsium, dan fosfor. Data dianalisis menggunakan uji ANAVA dua jalur dilanjutkan dengan uji LSD. Hasil analisis ANAVA dua jalur menujukkan bahwa jenis minuman dan lama perendaman berpengaruh bermakna terhadap kekerasan email gigi (p<0,05). Hasil uji LSD menunjukkan adanya perbedaan rerata yang signifikan (p<0,05) antar kelompok uji bir dan tuak. Kesimpulan dari penelitian ini adalah terdapat pengaruh jenis minuman beralkohol bir dan tuak dan lama perendaman terhadap kekerasan email gigi manusia (in vitro).Â ABSTRACT: The Effect Of Contact Time And Alcohol Beverages Beer And Tuak On Human Dental Enamel Hardness (In Vitro). Dental erosion is the loss of dental hard tissue, associated with acid. Acid type, low pH, and concentration of calcium, phosphate, and fluoride are being estimated as chemical factors of dental erosion. The purpose of this study was to determine the effect of contact time and alcohol beverages beer and tuak on human dental enamel hardness (in vitro).This study was using 14 samples maxillary first premolar. Each tooth was divided into two parts, buccal and palatal. Then divided into 7 treatment groups, i.e. groups of light beer âdrinkersâ (A1), moderate beer âdrinkersâ group (A2), heavy beer âdrinkerâ group (A3), light tuak âdrinkerâ group (B1), moderate tuak âdrinkersâ group (B2), the group âdrinkersâ heavy tuak (B3) and control group (C). Enamel hardness values were monitored using Micro Vickers Hardness Tester. Initial enamel hardness value was tested before the treatment. Maxilla first premolar teeth were exposed to beer and tuak for 10 seconds, 50 seconds, and 250 seconds per day for 30 days in the presence of artificial saliva. Final enamel hardness value was monitored after 30 days of treatment. Enamel hardness difference values were calculated by subtracting initial and final enamel hardness value. As supporting data, It was measured pH and concentration of calcium and phosphor in beer and tuak. Data was being analyzed by two-way ANOVA and LSD test. Results showed that contact time and alcohol beverage beer and tuak had a significant influence to enamel hardness value (p<0.05). LSD test showed that some groups had significant average difference (p<0.05). It was concluded that contact time and type of alcohol beverages beer and tuak had effect on human dental enamel hardness (in vitro).

Efek Minyak Atsiri Daun Kemangi (Ocimum Basilicum L.) Sebagai Agen Penghambat Pembentukan Biofilm Streptococcus Mutans Rosita Dwi Susanto, Like; Nuryanti, Archadian; Arie Wahyudi, Ivan
Insisiva Dental Journal Vol 2, No 1 (2013)
Publisher : Insisiva Dental Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar

Kemangi (Ocimum basilicum L.) merupakan tanaman yang sering digunakan untuk obat tradisional. KandunganÂ eugenol dalam minyak atsiri daun kemangi memiliki efek antibakteri terhadap bakteri Gram positif. Streptococcus mutans adalah bakteri anaerobik fakultatif Gram positif di dalam rongga mulut yang banyak ditemukan dalam plak biofilm. Penelitian ini bertujuan untuk mengetahui efek minyak atsiri daun kemangi (Ocimum basilicum L.) sebagai agen penghambat pembentukan biofilm Streptococcus mutans. Penelitian ini dimulai dari pengadaan daun kemangi, kemudian dilakukan destilasi uap air hingga didapat minyak atsiri. Konsentrasi minyakÂ atsiri yang digunakan dalam penelitian ini adalah 1%, 0,5%, 0,25%, 0,125%, dan 0,0625% dengan menggunakanÂ pelarut PEG 400 2,5%. Uji agen penghambat pembentukan biofilm dilakukan pada microplate round bottom 96Â wells dengan pewarnaan kristal violet 0,5%. Pembacaan nilai absorbansi menggunakan microplate readerdengan panjang gelombang 595nm. Data yang didapat diolah menggunakan rumus penghambatan pembentukanÂ biofilm. Daya penghambatan pembentukan biofilm dinyatakan sebagai nilai IC50 yang dianalisis menggunakanÂ metode Probit pada program SPSS versi 16. Hasil penelitian menunjukkan bahwa konsentrasi minyak atsiri daunÂ kemangi 0,0625%, 0,125%, 0,25%, 0,5%, dan 1% dapat menghambat pembentukan biofilm S. mutans sebesar4,451%, 40,121%, 80,416%, 88,586%, dan 94,812%. Kesimpulan dari penelitian ini adalah minyak atsiri daunÂ kemangi (Ocimum basilicum L.) memiliki efek sebagai agen penghambat pembentukan biofilm StreptococcusÂ mutans dengan IC50 pada konsentrasi 0,168%.

Pengaruh Lama Perendaman dan Jenis Minuman Beralkohol Bir dan Tuak terhadap Kekerasan Email Gigi Manusia (In Vitro) Malida Magista; Archadian Nuryanti; Ivan Arie Wahyudi
Majalah Kedokteran Gigi Indonesia Vol 21, No 1 (2014): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Erosi gigi merupakan hilangnya lapisan email gigi karena asam. Jenis asam, pH rendah, serta kandungan kalsium, fosfat, dan fluoride pada bir dan tuak diduga merupakan faktor kimiawi penyebab erosi gigi. Tujuan penelitian ini untuk mengetahui pengaruh lama perendaman dan jenis minuman beralkohol bir dan tuak terhadap kekerasan email gigi manusia (in vitro). Penelitian ini menggunakan 14 sampel gigi premolar pertama atas. Setiap gigi dibagi menjadi 2 bagian, bukal dan palatal. kemudian dibagi menjadi 7 kelompok perlakuan, yaitu kelompok (A1) perendaman dalam bir (ringan); (A2) kelompok perendaman dalam bir sedang; kelompok (A3) perendaman dalam bir berat, kelompok (B1): perendaman dalam tuak ringan, (B2): perendaman dalam tuak sedang, (B3): perendaman dalam tuak berat dan kelompok kontrol (C). Uji kekerasan email gigi dilakukan menggunakan Micro Vickers Hardness Tester. Pengujian kekerasan awal email gigi dilakukan sebelum perendaman gigi. Perendaman gigi premolar pertama atas pada bir dan tuak dilakukan selama 10 detik, 50 detik, dan 250 detik perhari dengan penyimpanan subjek penelitian pada saliva buatan. Uji kekerasan akhir email gigi dilakukan setelah perlakuan selama 30 hari. Nilai perubahan kekerasan email gigi merupakan selisih nilai kekerasan akhir dan nilai kekerasan awal email gigi. Sebagai tambahan data, pada bir dan tuak juga diukur kandungan pH, kalsium, dan fosfor. Data dianalisis menggunakan uji ANAVA dua jalur dilanjutkan dengan uji LSD. Hasil analisis ANAVA dua jalur menujukkan bahwa jenis minuman dan lama perendaman berpengaruh bermakna terhadap kekerasan email gigi (p<0,05). Hasil uji LSD menunjukkan adanya perbedaan rerata yang signifikan (p<0,05) antar kelompok uji bir dan tuak. Kesimpulan dari penelitian ini adalah terdapat pengaruh jenis minuman beralkohol bir dan tuak dan lama perendaman terhadap kekerasan email gigi manusia (in vitro).The Effect Of Contact Time And Alcohol Beverages Beer And Tuak On Human Dental Enamel Hardness (In Vitro). Dental erosion is the loss of dental hard tissue, associated with acid. Acid type, low pH, and concentration of calcium, phosphate, and fluoride are being estimated as chemical factors of dental erosion. The purpose of this study was to determine the effect of contact time and alcohol beverages beer and tuak on human dental enamel hardness (in vitro).This study was using 14 samples maxillary first premolar. Each tooth was divided into two parts, buccal and palatal. Then divided into 7 treatment groups, i.e. groups of light beer “drinkers” (A1), moderate beer “drinkers” group (A2), heavy beer “drinker” group (A3), light tuak “drinker” group (B1), moderate tuak “drinkers” group (B2), the group “drinkers” heavy tuak (B3) and control group (C). Enamel hardness values were monitored using Micro Vickers Hardness Tester. Initial enamel hardness value was tested before the treatment. Maxilla first premolar teeth were exposed to beer and tuak for 10 seconds, 50 seconds, and 250 seconds per day for 30 days in the presence of artificial saliva. Final enamel hardness value was monitored after 30 days of treatment. Enamel hardness difference values were calculated by subtracting initial and final enamel hardness value. As supporting data, It was measured pH and concentration of calcium and phosphor in beer and tuak. Data was being analyzed by two-way ANOVA and LSD test. Results showed that contact time and alcohol beverage beer and tuak had a significant influence to enamel hardness value (p<0.05). LSD test showed that some groups had significant average difference (p<0.05). It was concluded that contact time and type of alcohol beverages beer and tuak had effect on human dental enamel hardness (in vitro).

Pengaruh Lama Paparan Gelombang Ultrasonik Frekuensi Terapi terhadap Jumlah Koloni Bakteri Streptococcus mutans F. Fransiska; Archadian Nuryanti; Rini Maya Puspita
Majalah Kedokteran Gigi Indonesia Vol 19, No 1 (2012): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Latar Belakang. Gelombang Untrasonik telah digunakan untuk terapi dan diagnosis, di klinik kedokteran gigi popular digunakan untuk ultrasonik skaler. Efek termal dan nontermal gelombang ultrasonik dapat mempengaruhi lingkungan tumbuh bakteri, merusak enzim bakteri, dan struktur bakteri. Streptococcus mutans merupakan bakteri penyebab karies gigi. Tujuan penelitian. Penelitian ini bertujuan untuk mengetahui pengaruh lama paparan gelombang ultrasonik frekuensi terapi 3,5 MHz terhadap jumlah koloni bakteri Streptococcus mutans. Cara penelitian. Penelitian ini dilakukan pada 20 buah cawan petri yang berisi koloni bakteri streptococcus mutans dengan media agar darah. Dua puluh buah petri dibagi menjadi 4 kelompok yaitu kelompok kontrol (A) dan kelompok perlakuan (B, C, D). kelompok perlakuan diaplikasi gelombang ultrasonik frekuensi 3,5MHz selama 5, 10, dan 15 menit, sedangkan kelompok kontrol tidak diberi perlakuan apapun. Perhitungan jumlah koloni bakteri dilakukan dengan pengamatan menggunakan colony counter dengan standart plate count (SPC) method. Hasil penelitian jumlah koloni bakteri Streptococcus mutans dianalisis menggunakan ANAVA satu jalur menunjukkan ada perbedaan yang signifikan (p<0,05) berarti terdapat pengaruh lama paparan gelombang ultrasonik frekuensi terapi 3,5 MHz terhadap jumlah koloni bakteri Streptococcus mutans. Hasil analisis post hoc (LSD) terhadap jumlah koloni bakteri Streptococcus mutans juga menunjukkan ada perbedaan rerata antar kelompok perlawanan yang signifikan (p<0,05). Kesimpulan. Kesimpulan penelitian ini adalah lama paparan gelombang ultrasonik frekuensi terapi 3,5 MHz berpengaruh terhadap jumlah koloni bakteri Streptococcus mutans. Introduction. Ultrasonic waves have been used for therapy and diagnosis, in dental clinic ultrasonic waves are used popular for ultrasonic scaler. Thermal and non thermal effects from ultrasonic wave influence the environment of bacteria disturb bacteria enzyme and the bacteria structure. Streptococcus mutans is a bacterium that cause caries in teeth. The aim of this study was to observe the effect of duration of ultrasound in therapy frequency 3,5 MHz exposure towards Streptococcus mutans cell colony. Methods. This study was 20 petri dish with blood agar media containing the Streptococcus mutans cell colony. Twenty plates blood agar, were divided into 4 groups. Control group A was unexposed and treated group received ultrasound exposure at frecuency 3,5 MHz which for 5 minutes exposure (B), 10 minutes exposure (C) and 15 minutes exposure (D). Streptococcus mutans cell colony was countusing colony counter with standard plate count methos. Results. The results of one way ANOVA at 95o/o significance showed that duration ultrasonic therapy frecuency 3,5 MHz exposure influenced the amount of Streptococcus mutans cells colony (p<0,0,5). The post hoc test (LSD) showed the significantly mean difference (p,0,05) between groups. Conclusions. The conclusions, of this study is duration exposure ultrasonic therapy frecuency at 3,5 MHz influences on the amount of Streptococcus mutans cells colony.

Chewing gum supplemented with Brassica oleracea var. capitata f. rubra extract for pH detecting of artificial saliva Fifit Indriyantari; Aprilia Nur Pratiwi; Rizky Septiano Andian; Ida Bagus Alit Rai Sugiharta; Archadian Nuryanti
Majalah Kedokteran Gigi Indonesia Vol 6, No 3 (2020): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.37145

The prevalence of oral diseases in Indonesia is 25,9% in 2018, the highest percentage is recorded being caused to dental caries. Oral condition which may be acidic due to foods is potential to cause dental caries, as oral bacteria activities such as S. mutans may transform foods into becoming acids. In current condition, it is difficult for people to detect their own acid and base oral condition independently. This study aimed to create an innovation of a chewing gum supplemented with red cabbage (Brassica oleracea var. capitata f. rubra) extract which has functions as oral pH detector. The chewing gum was made by mixing the maceration extract of red cabbage and gumbase. This study was performed by using a qualitative test of pH 1-13 buffer solution colour change and pH 5-9 artificial saliva which was added by the extract, and then tested with manufactured chewing gum. Anthocyanin level was tested by using a 520 and 700 nm UV-Vis spectrophotometer. The study resulted in colour change in colour qualitative test. On pH 5-9 artificial saliva, colour change happened in order of orange, orange-brown, brown, brown-green, and green, after it was mixed with chewing gum. Colour compatibility test on chewing gum and artificial saliva showed a compatibility percentage of 80. Chewing gum supplemented with red cabbage can be used as pH detector of pH 5-9 artificial saliva.

EFFECTIVITY OF MULTIFUNCTION HERBAL TOOTHPASTE CONTAINING BAY LEAF (EUGENIA POLYANTHA WIGHT) EXTRACT AS EXTRINSIC STAIN REMOVAL ON TEETH AND DENTURE Mutiara Annisa; Archadian Nuryanti; Anne Handrini Dewi
ODONTO : Dental Journal Vol 9: Special Issue 1. April 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.0.40-50

Background: White teeth colour influence aesthetical appearance which leads to self-confidence and quality of life. Extrinsic stain is the most common aetiology of teeth discoloration, which caused by widely consumed products such as tea, coffee, and tobacco. It also formed on denture and cause negative impacts. Commercial extrinsic stain removal toothpastes only indicated for teeth and contain chemical active agent with undesirable effects. Aim of this study is to determine effectivity of bay leaf (Eugenia polyantha Wight) extract as toothpaste active agent for extrinsic stain removal on teeth and dentures.Method: Pictures of teeth, artificial teeth, and acrylic resin base taken before experiment, after stain induction (using 25g of tea in 100ml of 100oC water for 7 days, tea solution replaced every 24 hours), and after brushing. Toothpastes used for brushing were no active agent basic formulation toothpaste, bay leaf extract toothpastes at 5%, 10%, 15% concentrations, and commercial stain removal toothpaste as positive control. Specimens were brushed using automatic brushing machine for 70 seconds/surface, using 250g load, and speed 5 movements/sec. Colour index analysis of specimens’ pictures were computerized and analysed using CIELAB method to obtain L-value of extrinsic stain removal. Data analysis performed using one-way ANOVA (α=0.05).Result: There is effect of toothpaste containing bay leaf extract 15% to remove extrinsic stain both on teeth, artificial teeth, and acrylic resin base according to one-way ANOVA.Conclusion: Bay leaf extract is effective and potential to be used as toothpaste active agent for extrinsic stain removal on teeth and denture.

Efek Minyak Atsiri Daun Kemangi (Ocimum Basilicum L.) Sebagai Agen Penghambat Pembentukan Biofilm Streptococcus Mutans Like Rosita Dwi Susanto; Archadian Nuryanti; Ivan Arie Wahyudi
Insisiva Dental Journal: Majalah Kedokteran Gigi Insisiva Vol 2, No 1 (2013)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/di.v2i1.556

Kemangi (Ocimum basilicum L.) merupakan tanaman yang sering digunakan untuk obat tradisional. Kandungan eugenol dalam minyak atsiri daun kemangi memiliki efek antibakteri terhadap bakteri Gram positif. Streptococcus mutans adalah bakteri anaerobik fakultatif Gram positif di dalam rongga mulut yang banyak ditemukan dalam plak biofilm. Penelitian ini bertujuan untuk mengetahui efek minyak atsiri daun kemangi (Ocimum basilicum L.) sebagai agen penghambat pembentukan biofilm Streptococcus mutans. Penelitian ini dimulai dari pengadaan daun kemangi, kemudian dilakukan destilasi uap air hingga didapat minyak atsiri. Konsentrasi minyak atsiri yang digunakan dalam penelitian ini adalah 1%, 0,5%, 0,25%, 0,125%, dan 0,0625% dengan menggunakan pelarut PEG 400 2,5%. Uji agen penghambat pembentukan biofilm dilakukan pada microplate round bottom 96 wells dengan pewarnaan kristal violet 0,5%. Pembacaan nilai absorbansi menggunakan microplate readerdengan panjang gelombang 595nm. Data yang didapat diolah menggunakan rumus penghambatan pembentukan biofilm. Daya penghambatan pembentukan biofilm dinyatakan sebagai nilai IC50 yang dianalisis menggunakan metode Probit pada program SPSS versi 16. Hasil penelitian menunjukkan bahwa konsentrasi minyak atsiri daun kemangi 0,0625%, 0,125%, 0,25%, 0,5%, dan 1% dapat menghambat pembentukan biofilm S. mutans sebesar4,451%, 40,121%, 80,416%, 88,586%, dan 94,812%. Kesimpulan dari penelitian ini adalah minyak atsiri daun kemangi (Ocimum basilicum L.) memiliki efek sebagai agen penghambat pembentukan biofilm Streptococcus mutans dengan IC50 pada konsentrasi 0,168%.

The effects of ultrasonic scaling duration and replication on caspase-3 expression of Sprague Dawley rat's pulp cells Archadian Nuryanti; Marsetyawan HNE Soesatyo; Dewi Agustina; Siti Sunarintyas
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 1 (2015): March 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Ultrasonic scaling has been used commonly for stain and calculus removal in dental clinic for over 60years. Previous researches even had proved that ultrasonic scaling may give effects on the surface of tooth root. Ultrasonic wave exposure for 20 seconds or more can increase caspase-3 activity as an indicator of increased apoptotic cells associated with tissue damage. Purpose: This research was aimed to investigate the effects of ultrasonic scaling duration and replication on caspace-3 expression in dental pulp cells. Methods: The samples of this research were 54 male Sprague Dawley rats aged 2 months old divided into 2 groups, each of which consisted of 27 mice. The first group was induced with stain, while the second group was not. Each group was divided into 3 subgroups for ultrasonic scaling 1, 3, and 5 times. Each subgroup was divided into 3 sub-subgroups for duration procedure of 15, 30 and 60 seconds respectively. During scaling process, those rats were anesthetized using 0.1 ml of ketamine and 0.1 ml of xylol added to 2 ml of distilled water injected intramuscularly into their right thigh as much as 0.4 ml. Scaling was done on buccal surface of right first maxillary molar from cervical to occlusal. The teeth were decalcified and embedded in paraffin, then their sagittal plane was cut for thickness of 3µm and painted with immunohystochemistry for detecting caspace-3 expression of cell within dental pulp. Results: The results showed that the duration and replication of ultrasonic scaling procedures affected on the expression of caspace-3 cells as analyzed with Univariate Analisis of Variance test (p<0.05). Conclusion: It can be concluded that duration and replication of ultrasonic scaling procedure on teeth with and without stain enhauced the expression of caspace-3 in dental pulp cells.

EFFECTIVITY OF MULTIFUNCTION HERBAL TOOTHPASTE CONTAINING BAY LEAF (EUGENIA POLYANTHA WIGHT) EXTRACT AS EXTRINSIC STAIN REMOVAL ON TEETH AND DENTURE Mutiara Annisa; Archadian Nuryanti; Anne Handrini Dewi
Odonto : Dental Journal Vol 9: Special Issue 1. April 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: White teeth colour influence aesthetical appearance which leads to self-confidence and quality of life. Extrinsic stain is the most common aetiology of teeth discoloration, which caused by widely consumed products such as tea, coffee, and tobacco. It also formed on denture and cause negative impacts. Commercial extrinsic stain removal toothpastes only indicated for teeth and contain chemical active agent with undesirable effects. Aim of this study is to determine effectivity of bay leaf (Eugenia polyantha Wight) extract as toothpaste active agent for extrinsic stain removal on teeth and dentures.Method: Pictures of teeth, artificial teeth, and acrylic resin base taken before experiment, after stain induction (using 25g of tea in 100ml of 100oC water for 7 days, tea solution replaced every 24 hours), and after brushing. Toothpastes used for brushing were no active agent basic formulation toothpaste, bay leaf extract toothpastes at 5%, 10%, 15% concentrations, and commercial stain removal toothpaste as positive control. Specimens were brushed using automatic brushing machine for 70 seconds/surface, using 250g load, and speed 5 movements/sec. Colour index analysis of specimens’ pictures were computerized and analysed using CIELAB method to obtain L-value of extrinsic stain removal. Data analysis performed using one-way ANOVA (α=0.05).Result: There is effect of toothpaste containing bay leaf extract 15% to remove extrinsic stain both on teeth, artificial teeth, and acrylic resin base according to one-way ANOVA.Conclusion: Bay leaf extract is effective and potential to be used as toothpaste active agent for extrinsic stain removal on teeth and denture.

The comparison of two programmes to measure color difference (Î”E) from tooth sample photo Mutiara Annisa; Harsini Harsini; Archadian Nuryanti
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.257-267

Background: Color evaluation is crucial to evaluate a materialâ€™s quality. One alternative method for evaluating materialâ€™s color is using photographs analysed by software. This research evaluates the use of digital imaging and software (ImageJ and Photoshop) to obtain color differences of tooth sample in-vitro. Method: The sample used is bovine teeth that were given tea-staining and brushing treatment using five tested toothpastes. Sampleâ€™s photographs for each toothpasteâ€™s group (n=4) were taken before and after experiment. DLSR Nikon D90 was used with digital CCD censor, macro lens 105mm, manual setting (ISO 200, F-Stop 5, Shutter Speed 1/400) with distance to sample of 25cm. Sample was positioned in foldable mini-studio-box (24.5x24.5x22.5cm) with LED-lighting (6500-7000 color temperature). The photographs were taken in close room at 11.00am. Two software were used to obtain the color value from pre- and post-experimental photographs of the sample at the middle-third of the sample using CIE-Lab (Commision Internacional de lâ€™Eclairage L*a*b) color system. The resulted color difference (Î”E) value of sample from the two-software were compared using independent T-test and evaluate the measurement accuracy using Pearsonâ€™s correlation (Î±=0.05). Result: ImageJ and Photoshop analyses of the sample photographs yield comparable Î”E values, as determined by an independent T-test (p=0.893). The Pearson correlation test reveals a positive correlation (R=0.904) between the two software. Conclusion: The use of digital photography and software to obtain Î”E values are accurate, representative, and recommended when taking into account the controlled procedure of photographing the sample and analysing the sample's color value.

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