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Kontrasepsi Hormonal Meningkatkan Kadar Î±-Amylase Saliva Handajani, Juni; Puspita, Rini Maya; Amelia, Rizki
Majalah Kedokteran Gigi Indonesia Vol 21, No 1 (2014)
Publisher : Majalah Kedokteran Gigi Indonesia

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Salivary Î±-amylase atau Î±-amylase saliva (SAA) adalah salah satu enzim dalam saliva yang berperan penting pada inisiasi digesti karbohidrat dan fungsi interaksi bakteri. Kontrasepsi hormonal sangat populer di Indonesia untuk mencegah kehamilan. Penelitian ini bertujuan untuk mengetahui kadar SAA wanita pemakai kontrasepsi pil dan suntik. Subjek penelitian sebanyak 30 perempuan usia 20-35 tahun. Prosedur penelitian telah mendapat persetujuan dari Komite Etik Fakultas Kedokteran Universitas Gadjah Mada Yogyakarta. Subjek dibagi menjadi 3 kelompok (pemakai kontrasepsi pil, suntik, dan kontrol), masing-masing 10 perempuan. Kriteria subjek antara lain subjek sehat, tidak menggunakan alat ortodontik, protesa atau mahkota, serta menggunakan kontrasepsi hormonal lebih dari 3 bulan. Sampel saliva dikumpulkan pada sore hari (16.00-18.00 WIB) selama 1 menit dengan metode tanpa stimulasi. Kadar tingkat SAA diukur menggunakan ELISA kit (Salimetrics LLC) dengan Optical Density (OD) pada 405 nm. Data dianalisis menggunakan ANOVA (p<0,05). Hasil penelitian menunjukkan kadar SAA tertinggi pada perempuan pemakai kontrasepsi Â pil dan ada perbedaan yang signifikan diantara tiga kelompok. Disimpulkan bahwa kontrasepsi hormonal meningkatkan kadar SAA.ABSTRACT: Hormonal Contraceptive Increased The Level of Salivary Î-Amylase. Salivary Î±-amylase (SAA) is one of the most important enzymes in saliva. This enzyme was mainly involved in the initiation of the digestion of starch in the oral cavity and has significant bacterial interactive function. Hormonal contraceptives are very popular in Indonesia to avoid pregnancy. This study aimed to evaluate the level of SAA in woman who taking pill and by injection contraceptives. Thirty women were in subjects, 20-35 years old, approval ethical clearance from Ethic Committee Medical Faculty of Gadjah Mada University, Yogyakarta Indonesia. Subjects were divided into three groups (taking pill contraceptive, by injection contraceptive and control). Each group consisted ten women. Criteria for issue were medication free, healthy, no orthodontic treatment, no prosthesis or crown and took hormonal contraceptives more than three months. Saliva samples were collected at afternoon (16.00-18.00 pm) for 1 minute using unstimulating method. The level of SAA was measured by ELISA kit (Salimetrics LLC). Optical Density was read on a standard plate at 405 nm. Data for level SAA were analyzed using ANOVA (p<0.05). Results showed the highest level of SAA in woman who takes pill contraceptive, and there were significant differences between the three groups. This study is suggesting that the hormonal contraceptive increased the level of SAA.

PEMAKAIAN KONTRASEPSI PIL DAN SUNTIK MENAIKKAN pH DAN VOLUME SALIVA: CONTRACEPTIVE PILL AND INJECTION INCREASE pH AND VOLUME OF SALIVA Juni Handajani; Rini Maya Puspita; Rizki Amelia
Dentika: Dental Journal Vol. 15 No. 1 (2010): Dentika Dental Journal
Publisher : TALENTA

Saliva is the mixture of oral fluid, that has function to protect the oral cavity. Hormonal contraception is very popular in Indonesia to avoid pregnancy. This study aimed to evaluate salivary volume and pH of woman who taking contraceptive pill and by injection. The design of study was a cross sectional . The subjects were 30 women, 20-35 years old, with the approval of ethical clearance from Ethic Comittee Medical Faculty of Gadjah Mada University. The subjects were divided into 3 groups (taking contraceptive pill, by contraceptive injection and control) that each group consisted of 10 women. The criteria of subjects were healthy, no orthodontic treatment, no prothesa or crown, and taking hormonal contraception more than 3 months. Saliva samples were collected in the afternoon (16.00-18.00 pm) for 1 minute using unstimulating method. Data analysis using ANOVA test (p<0,05). The results showed that there were significant differences in salivary pH and volume. As conclusion, hormonal contraceptive can increase salivary pH and volume.

The effects of regular exercise on the number of osteoclasts in ovariectomized Sprague dawley rat mandible Rini Maya Puspita
Biomedical Engineering Vol 1, No 1 (2015): BIOMEDICAL ENGINEERING
Publisher : Biomedical Engineering

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Objective: This study aimed to examine the effect of regular exercise on the number of osteoclasts inovariectomized Sprague Dawley rat mandible.Methods: Eight female Sprague Dawley rats, 3 months of age, were ovariectomized and divided into control(untreated) and treatment group (regular exercise). The exercise conducted by the rats ran on a speciallydesigned rat treadmill for 12 weeks, 5 times per week, at a speed of 10-18 m/min for 60 minutes per day. At theend of the treatment, the mandible was taken and cleaned out from the tissue for making the histologicalpreparations with Hematoxylin and Eosin (HE) stain. The number of osteoclasts counted from each histologicalpreparation on 10 visual fields and the obtained data were analyzed using independent t-test.Result: The number of osteoclast was lower significantly in ovariectmized rats compared to control group (P <0.001).Conclusion: Regular exercise can decrease the number of osteoclasts in ovariectomized Sprague Dawley ratmandibles.

The effects of regular exercise on the number of osteoclasts in ovariectomized Sprague dawley rat mandibles Rini Maya Puspita; Fitrina Rachmadanty Siregar
Biomedical Engineering Vol 1, No 1 (2014)
Publisher : Biomedical Engineering

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Objective: This study aimed to examine the effect of regular exercise on the number of osteoclasts in ovariectomized Sprague Dawley rat mandibles.Methods: Eight female Sprague Dawley rats, 3 months of age, were ovariectomized and divided into control (untreated) and treatment group (regular exercise). The exercise conducted by the rats ran on a specially designed rat treadmill for 12 weeks, 5 times per week, at a speed of 10-18 m/min for 60 minutes per day. At the end of the treatment, the mandible was taken and cleaned out from the tissue for making the histological preparations with Hematoxylin and Eosin (HE) stain. The number of osteoclasts counted from each histological preparation on 10 visual fields and the obtained data were analyzed using independent t-test.Result: The number of osteoclast was lower significantly in ovariectmized rats compared to control group (P < 0.001). Conclusion: Regular exercise can decrease the number of osteoclasts in ovariectomized Sprague Dawley rat mandibles.Key Words: mandibles; menopause; regular exercise; the number of osteoclasts;

Kontrasepsi Hormonal Meningkatkan Kadar α-Amylase Saliva Juni Handajani; Rini Maya Puspita; Rizki Amelia
Majalah Kedokteran Gigi Indonesia Vol 21, No 1 (2014): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Salivary α-amylase atau α-amylase saliva (SAA) adalah salah satu enzim dalam saliva yang berperan penting pada inisiasi digesti karbohidrat dan fungsi interaksi bakteri. Kontrasepsi hormonal sangat populer di Indonesia untuk mencegah kehamilan. Penelitian ini bertujuan untuk mengetahui kadar SAA wanita pemakai kontrasepsi pil dan suntik. Subjek penelitian sebanyak 30 perempuan usia 20-35 tahun. Prosedur penelitian telah mendapat persetujuan dari Komite Etik Fakultas Kedokteran Universitas Gadjah Mada Yogyakarta. Subjek dibagi menjadi 3 kelompok (pemakai kontrasepsi pil, suntik, dan kontrol), masing-masing 10 perempuan. Kriteria subjek antara lain subjek sehat, tidak menggunakan alat ortodontik, protesa atau mahkota, serta menggunakan kontrasepsi hormonal lebih dari 3 bulan. Sampel saliva dikumpulkan pada sore hari (16.00-18.00 WIB) selama 1 menit dengan metode tanpa stimulasi. Kadar tingkat SAA diukur menggunakan ELISA kit (Salimetrics LLC) dengan Optical Density (OD) pada 405 nm. Data dianalisis menggunakan ANOVA (p<0,05). Hasil penelitian menunjukkan kadar SAA tertinggi pada perempuan pemakai kontrasepsi pil dan ada perbedaan yang signifikan diantara tiga kelompok. Disimpulkan bahwa kontrasepsi hormonal meningkatkan kadar SAA.Hormonal Contraceptive Increased The Level of Salivary Α-Amylase. Salivary α-amylase (SAA) is one of the most important enzymes in saliva. This enzyme was mainly involved in the initiation of the digestion of starch in the oral cavity and has significant bacterial interactive function. Hormonal contraceptives are very popular in Indonesia to avoid pregnancy. This study aimed to evaluate the level of SAA in woman who taking pill and by injection contraceptives. Thirty women were in subjects, 20-35 years old, approval ethical clearance from Ethic Committee Medical Faculty of Gadjah Mada University, Yogyakarta Indonesia. Subjects were divided into three groups (taking pill contraceptive, by injection contraceptive and control). Each group consisted ten women. Criteria for issue were medication free, healthy, no orthodontic treatment, no prosthesis or crown and took hormonal contraceptives more than three months. Saliva samples were collected at afternoon (16.00-18.00 pm) for 1 minute using unstimulating method. The level of SAA was measured by ELISA kit (Salimetrics LLC). Optical Density was read on a standard plate at 405 nm. Data for level SAA were analyzed using ANOVA (p<0.05). Results showed the highest level of SAA in woman who takes pill contraceptive, and there were significant differences between the three groups. This study is suggesting that the hormonal contraceptive increased the level of SAA.

Pengaruh Lama Paparan Gelombang Ultrasonik Frekuensi Terapi terhadap Jumlah Koloni Bakteri Streptococcus mutans F. Fransiska; Archadian Nuryanti; Rini Maya Puspita
Majalah Kedokteran Gigi Indonesia Vol 19, No 1 (2012): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Latar Belakang. Gelombang Untrasonik telah digunakan untuk terapi dan diagnosis, di klinik kedokteran gigi popular digunakan untuk ultrasonik skaler. Efek termal dan nontermal gelombang ultrasonik dapat mempengaruhi lingkungan tumbuh bakteri, merusak enzim bakteri, dan struktur bakteri. Streptococcus mutans merupakan bakteri penyebab karies gigi. Tujuan penelitian. Penelitian ini bertujuan untuk mengetahui pengaruh lama paparan gelombang ultrasonik frekuensi terapi 3,5 MHz terhadap jumlah koloni bakteri Streptococcus mutans. Cara penelitian. Penelitian ini dilakukan pada 20 buah cawan petri yang berisi koloni bakteri streptococcus mutans dengan media agar darah. Dua puluh buah petri dibagi menjadi 4 kelompok yaitu kelompok kontrol (A) dan kelompok perlakuan (B, C, D). kelompok perlakuan diaplikasi gelombang ultrasonik frekuensi 3,5MHz selama 5, 10, dan 15 menit, sedangkan kelompok kontrol tidak diberi perlakuan apapun. Perhitungan jumlah koloni bakteri dilakukan dengan pengamatan menggunakan colony counter dengan standart plate count (SPC) method. Hasil penelitian jumlah koloni bakteri Streptococcus mutans dianalisis menggunakan ANAVA satu jalur menunjukkan ada perbedaan yang signifikan (p<0,05) berarti terdapat pengaruh lama paparan gelombang ultrasonik frekuensi terapi 3,5 MHz terhadap jumlah koloni bakteri Streptococcus mutans. Hasil analisis post hoc (LSD) terhadap jumlah koloni bakteri Streptococcus mutans juga menunjukkan ada perbedaan rerata antar kelompok perlawanan yang signifikan (p<0,05). Kesimpulan. Kesimpulan penelitian ini adalah lama paparan gelombang ultrasonik frekuensi terapi 3,5 MHz berpengaruh terhadap jumlah koloni bakteri Streptococcus mutans. Introduction. Ultrasonic waves have been used for therapy and diagnosis, in dental clinic ultrasonic waves are used popular for ultrasonic scaler. Thermal and non thermal effects from ultrasonic wave influence the environment of bacteria disturb bacteria enzyme and the bacteria structure. Streptococcus mutans is a bacterium that cause caries in teeth. The aim of this study was to observe the effect of duration of ultrasound in therapy frequency 3,5 MHz exposure towards Streptococcus mutans cell colony. Methods. This study was 20 petri dish with blood agar media containing the Streptococcus mutans cell colony. Twenty plates blood agar, were divided into 4 groups. Control group A was unexposed and treated group received ultrasound exposure at frecuency 3,5 MHz which for 5 minutes exposure (B), 10 minutes exposure (C) and 15 minutes exposure (D). Streptococcus mutans cell colony was countusing colony counter with standard plate count methos. Results. The results of one way ANOVA at 95o/o significance showed that duration ultrasonic therapy frecuency 3,5 MHz exposure influenced the amount of Streptococcus mutans cells colony (p<0,0,5). The post hoc test (LSD) showed the significantly mean difference (p,0,05) between groups. Conclusions. The conclusions, of this study is duration exposure ultrasonic therapy frecuency at 3,5 MHz influences on the amount of Streptococcus mutans cells colony.

Analisis sitogenetik sel epitel mukosa bukal pekerja stasiun pengisi bahan bakar umum di kota Yogyakarta Regina TC. Tandelilin; Elastria Widita; Rini Maya Puspita; Tang Sze Mun
Jurnal Teknosains Vol 10, No 2 (2021): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/teknosains.43852

Workers employed in petroleum station have a high-risk exposure to a wide range of toxic compounds with known mutagenic and carcinogenic potential. Cytogenetic damage might have happened if they continuously exposed to petroleum derivatives. This study aimed to analyse the cytogenetic damage in exfoliated buccal cells among petroleum station workers in Yogyakarta City. This cross-sectional study was carried out on 30 petrol station workers who are working at a different petrol station in Yogyakarta and the control group consisted of 30 healthy subjects. Examination for all subjects included frequencies of nuclear abnormalities, including pycnosis, karyorrhexis, and karyolysis. Cytological preparations were stained according to papanicolaou reaction and analyzed under light microscope for making a score for degenerative nuclear alterations (pycnosis, karyolysis and karyorrhexis). Analysis of buccal cells revealed that frequencies of pycnosis and karyorrhexis in petrol station workers were significantly higher than the control group (P<0.05). Conversely, there was no significant difference in karyolisis among groups. These findings indicate that the petrol station workers are under the risk of significant cytogenetic damage, particularly pycnosis and karyorrhexis.

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