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All Journal The Journal of Experimental Life Sciences (JELS) Research Journal of Life Science Journal of Environmental Engineering and Sustainable Technology Indonesian Journal of Tropical Aquatic

Arief Prajitno, Arief

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Author-ID : 523710

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Decision Sciences, Operations Research & Management Energy Environmental Science Immunology & microbiology Mechanical Engineering Medicine & Pharmacology Neuroscience

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Articles

Minimum Inhibitory Concentration of Marine Microalgae Dunaliella salina on Fish Pathogenic Bacteria Edwardsiella tarda Rusmawanto, Rusmawanto; Prajitno, Arief; Yuniarti, Ating
Research Journal of Life Science Vol 6, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Dunaliella salina is a type of marine microalgae. The objective of this research were investigated the effect of D. salina to inhibit the growth of E. tarda. The method used in this study was examined antibacterial activity of D. salina using disc diffusion and co culture test. The secondary metabolite compound in D. salina was tested using phytochemical screening and FTIR methods. The results obtained D. salina extracted using n-hexane showed the best activity for inhibiting the growth of E. tarda. Minimum concentration of 100 mg.L-1 crude extract can inhibit E. tarda with a total of bacterial colonies 137x1011 CFU.mL-1. The phytochemical test results showed that D. salina extract using n-hexane contained flavonoids, saponins, alkaloids, terpenoids and phenols. The FTIR analysis showed phenol and carotene compounds are one of the secondary metabolites that can be used as antibacterial. It can be concluded that the extract has strong antibacterial activity against E. tarda and potentially as antibacterial in aquaculture.

ENHANCEMENT GLUTATHIONE PEROXIDASE ACTIVITY AND Î±2-MACROGLOBULIN GENE EXPRESSION OF MACROBRACHIUM ROSENBERGII FED WITH AQUEOUS MORINDA CITRIFOLIA LEAVES EXTRACT-SUPPLEMENTED DIET Halim, Atika Marisa; Prajitno, Arief; Chang, Chin Chyuan
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Publisher : University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v1i1.4849

Morinda citrifolia, known commercially as noni is often used for enhance immunity, these plant rich of phenolic compound with antioxidant properties. In the present study, Macrobrachium rosenbergii were fed with diets containing of aqueous M. citrifolia leaves extract (AMLE) at 0.6, 4 and 6 g kg-1. Glutathione peroxidase (GPx) activity was conducted to measure immune parameter, these parameter was evaluated before and after 7, 21, 35, 49 and 63 days of feeding trial. Immune gene expression of ?2-macroglubulin (?2-M) was evaluated in this study. The results showed that after 63 days of feeding treatment, significantly increased in GPx activity. Gene expressions of ?2-macroglubulin was significantly upregulated. These results recommend that administration of AMLE can be used as an immunostimulant and regulated immune response and immune gene expression in M. rosenbergii. Keyword: Morinda citrifolia, Macrobrachium rosenbergii, GPx, ?2-M

IN-VITRO INHIBITION TEST OF SINTRONG LEAVES (CRASSOCEPHALUM CREPIDIOIDES) CRUDE EXTRACT ON EDWARDSIELLA ICTALURI BACTERIA Nova, Jely; Suprastyani, Heny; Prajitno, Arief
Journal of Environmental Engineering and Sustainable Technology Vol 9, No 01 (2022)
Publisher : Directorate of Research and Community Service (DRPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jeest.2022.009.01.3

Parasites, viruses, and bacteria contribute to the transmission of infectious diseases. Catfish Enteric Septicemia (ESM) is caused by the bacterium Edwardsiella ictaluri. Antibiotics are used to treat this disease. However, the continuous use of antibiotics will lead to antibiotic resistance in fish and the contamination of the environment. Therefore, it is vital to have an alternative containing natural antibacterial components, such as Sintrong Leaf (Crassocephalum crepidioides). The composition of sintrong leaves consists of flavonoids, alkaloids, saponins, and tannins. This study employed an experimental method with a completely randomized design (CRD), which included five treatments and three replications. Treatment dosages of 25 ppm, 50 ppm, 75 ppm, 100 ppm, and 125 ppm were utilized along with the positive control (doxycycline) and negative control (without treatment). The investigation revealed that a crude extract of sintrong leaf exhibited an inhibitory effect that was effective against E. ictaluri bacteria. The equation pattern indicated y = -0.0004x2 + 0.0716x + 6.933 with an R2 coefficient of 0.71 showing a quadratic graph as the outcome. The graph results show that the best dose of crude extract from sintrong leaves against E. ictaluri bacteria is 89.5 ppm.

Phytochemical Screening and Antimicrobial Activity of Roselle (Hibiscus sabdariffa L.) Flower Extract Against Aeromonas hydrophila Bariyyah, Sitti Khairul; Prajitno, Arief; Yuniarti, Ating
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Graduate School, Universitas Brawijaya

Medicinal plants as an antimicrobial agent may provide an alternative way to replace the use of antibiotics to control disease agents in aquaculture. Roselle flowers (Hibiscus sabdariffa L.) has been used in many sectors as a source of functional food, natural coloring agents as well as antimicrobial agents. The objectives of this study were to evaluate the phytochemical compound in methanolic extract of roselle flower and their antimicrobial activity against Aeromonas hydrophila. The phytochemical composition of roselle flower was evaluated using phytochemical screening and FTIR. While the antimicrobial activity was performed by using the disc diffusion agar and co-culture with A. hydrophila. The results of phytochemical screening confirmed the presence of alkaloids, flavonoids, saponins, steroids, triterpenoids, and tannins. The results of FTIR revealed that Roselle flower extract had the main phenolic compounds. The result of disc diffusion and co-culture method indicated that the roselle flower extract had antibacterial activity against A. hydrophila. This antibacterial activity depended on the concentration applied.Keywords: antibiotics, Co-culture, Disk diffusion, flavonoid, Hibiscus sp.

Phytochemicals and The Ability of Plantago major Linn. Extract to Inhibit The growth of Aeromonas hydrophila Dewi, Annisa Farhana; Prajitno, Arief; Yuniarti, Ating
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Graduate School, Universitas Brawijaya

Aeromonas hydrophila is a type of gram-negative bacteria that can harm humans and major animals that have poikilotherm properties such as fish and shrimp. These bacteria can cause haemorrhages in fish until death and causing fish farming production to failure. The use of medicinal plants has been trusted by people all over the world to overcome various disease problems, one of which is a disease caused by bacteria. Plantago major L. is known that able to inhibit the growth of both gram-positive and gram-negative bacteria. The purposes of this study were to identify the extracted compounds contained in Plantago major L., using FTIR and UV-VIS spectrophotometer and to evaluate antibacterial activity against A. hydrophila. The results of this study showed that crude extracts of Plantago major L. contained polar compounds such as phenols, flavonoids, saponins, and tannins. The presence of phenols and flavonoids was confirmed by the results of FTIR and UV-VIS. Furthermore, the crude extract of Plantago major L. significantly inhibited the growth of A. hydrophila (P <0.05).Keywords: Antibacterial, Co-culture, Extraction, FTIR, UV-VIS.

Co-Authors Ating Yuniarti Bariyyah, Sitti Khairul Chang, Chin Chyuan Dewi, Annisa Farhana Halim, Atika Marisa Heny Suprastyani, Heny Nova, Jely Rusmawanto, Rusmawanto

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