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All Journal Research Journal of Life Science Journal of Environmental Engineering and Sustainable Technology Indonesian Journal of Tropical Aquatic
Arief Prajitno, Arief
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Decision Sciences, Operations Research & Management Energy Environmental Science Mechanical Engineering Medicine & Pharmacology

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Minimum Inhibitory Concentration of Marine Microalgae Dunaliella salina on Fish Pathogenic Bacteria Edwardsiella tarda Rusmawanto, Rusmawanto; Prajitno, Arief; Yuniarti, Ating
Research Journal of Life Science Vol 6, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.624 KB) | DOI: 10.21776/ub.rjls.2019.006.02.1

Abstract

Dunaliella salina is a type of marine microalgae. The objective of this research were investigated the effect of D. salina to inhibit the growth of E. tarda. The method used in this study was examined antibacterial activity of D. salina using disc diffusion and co culture test. The secondary metabolite compound in D. salina was tested using phytochemical screening and FTIR methods. The results obtained D. salina extracted using n-hexane showed the best activity for inhibiting the growth of E. tarda. Minimum concentration of 100 mg.L-1 crude extract can inhibit E. tarda with a total of bacterial colonies 137x1011 CFU.mL-1. The phytochemical test results showed that D. salina extract using n-hexane contained flavonoids, saponins, alkaloids, terpenoids and phenols. The FTIR analysis showed phenol and carotene compounds are one of the secondary metabolites that can be used as antibacterial. It can be concluded that the extract has strong antibacterial activity against E. tarda and potentially as antibacterial in aquaculture.
ENHANCEMENT GLUTATHIONE PEROXIDASE ACTIVITY AND α2-MACROGLOBULIN GENE EXPRESSION OF MACROBRACHIUM ROSENBERGII FED WITH AQUEOUS MORINDA CITRIFOLIA LEAVES EXTRACT-SUPPLEMENTED DIET Halim, Atika Marisa; Prajitno, Arief; Chang, Chin Chyuan
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Publisher : University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v1i1.4849

Abstract

Morinda citrifolia, known commercially as noni is often used for enhance immunity, these plant rich of phenolic compound with antioxidant properties. In the present study, Macrobrachium rosenbergii were fed with diets containing of aqueous M. citrifolia leaves extract (AMLE) at 0.6, 4 and 6 g kg-1. Glutathione peroxidase (GPx) activity was conducted to measure immune parameter, these parameter was evaluated before and after 7, 21, 35, 49 and 63 days of feeding trial. Immune gene expression of ?2-macroglubulin (?2-M) was evaluated in this study. The results showed that after 63 days of feeding treatment, significantly increased in GPx activity. Gene expressions of ?2-macroglubulin was significantly upregulated. These results recommend that administration of AMLE can be used as an immunostimulant and regulated immune response and immune gene expression in M. rosenbergii. Keyword: Morinda citrifolia, Macrobrachium rosenbergii, GPx, ?2-M
IN-VITRO INHIBITION TEST OF SINTRONG LEAVES (CRASSOCEPHALUM CREPIDIOIDES) CRUDE EXTRACT ON EDWARDSIELLA ICTALURI BACTERIA Nova, Jely; Suprastyani, Heny; Prajitno, Arief
Journal of Environmental Engineering and Sustainable Technology Vol 9, No 01 (2022)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jeest.2022.009.01.3

Abstract

Parasites, viruses, and bacteria contribute to the transmission of infectious diseases. Catfish Enteric Septicemia (ESM) is caused by the bacterium Edwardsiella ictaluri. Antibiotics are used to treat this disease. However, the continuous use of antibiotics will lead to antibiotic resistance in fish and the contamination of the environment. Therefore, it is vital to have an alternative containing natural antibacterial components, such as Sintrong Leaf (Crassocephalum crepidioides). The composition of sintrong leaves consists of flavonoids, alkaloids, saponins, and tannins. This study employed an experimental method with a completely randomized design (CRD), which included five treatments and three replications. Treatment dosages of 25 ppm, 50 ppm, 75 ppm, 100 ppm, and 125 ppm were utilized along with the positive control (doxycycline) and negative control (without treatment). The investigation revealed that a crude extract of sintrong leaf exhibited an inhibitory effect that was effective against E. ictaluri bacteria. The equation pattern indicated y = -0.0004x2 + 0.0716x + 6.933 with an R2 coefficient of 0.71 showing a quadratic graph as the outcome. The graph results show that the best dose of crude extract from sintrong leaves against E. ictaluri bacteria is 89.5 ppm.
Co-Authors Ating Yuniarti Chang, Chin Chyuan Halim, Atika Marisa Heny Suprastyani, Heny Nova, Jely Rusmawanto, Rusmawanto