Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal Jurnal Ilmu Ternak Veteriner Journal of Biodiversity and Biotechnology
Aprizal Panus, Aprizal
Institute of Veterinary Subang, FKH IPB
Agriculture, Biological Sciences & Forestry Veterinary

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Newcastle Disease Virus infection study on duck and chicken in Subang district Panus, Aprizal; Setiyaningsih, S.; Mayasari, N.L.P.I.
Indonesian Journal of Animal and Veterinary Sciences Vol 20, No 2 (2015): JUNE 2015
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (706.973 KB) | DOI: 10.14334/jitv.v20i2.1168

Abstract

The objectives of this research were to study Newcastle Disease Virus (NDV) infection in Subang area and to examine the diversity of the circulating NDV. Swabs of cloacal and oropharynx, and serum were sampled from total of 393 chickens and 149 ducks in backyard farms and live bird markets located in 10 subdistricts. Screening of NDV in pool of 5-7 samples by real-time Reverse-Transcription Polymerase Chain Reaction (rRT-PCR) matrix (M) showed 19/67 (28.3%) cloacal and 8/67 (11.9%) pharyngeal pools of chicken samples; 18/67 (26.9%) of the pools excreted virus via cloaca and oropharynx, while the duck pools of 8/30 (26.7%) shed virus from cloaca. Virus isolation attempted on individual sample from positive pools yielded 18 isolates which the majority of the isolates showed homogeneous antigenic character, only some of these showed variations up to 2 Log2 with Lasota and 4 Log2 with Komarov antisera. Majority of isolates had a higher affinity to Komarov indicating their propencity to virulent strains. Pathogenicity examination using elution test showed 3 isolates virus were grouped to mesogenic strains and 15 isolates to velogenic strain, in agreement with rRT-PCR fusion results. HI test on 408 sera showed that NDV antibody was detected in 48 (12%) birds with titres ranging from 1 to 8 Log2; only about 13% of vaccinated chickens demonstrated protective antibody titre (≥3 Log2). Newcastle disease is still endemic in Subang with relatively low antigenic variation among circulating strains. Key Words: Newcastle Disease, rRT-PCR Detection, Detection of Virulence, Antigenic Diversity, Antibody
Risk Factors for H9N2 Contamination in Live Bird Markets of the Greater Jakarta, Indonesia Aisah, Rahma Hani; Sumiarto, Bambang; Wibawa, Hendra; Nugroho, Widagdo Sri; Panus, Aprizal; Sodirun, Sodirun
Journal of Biodiversity and Biotechnology Vol 3, No 1 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i1.74263

Abstract

Low Pathogenicity Avian Influenza virus subtype H9N2 causes decreased egg production and two of the four defined H9N2 poultry lineages (G1 and Y280) have been associated with human infections. The high positive test results for the H9N2 virus in the LBMs of the Greater Jakarta area in 2021 can be a source of transmission for the surrounding area. This study aims to determine the prevalence and risk factors that play a role in the H9N2 contamination in the LBMs to obtain a multivariate analysis model. A cross-sectional study was conducted on 87 vendors and 124 broiler chickens from 44 LBMs randomly selected in Tangerang City, North Jakarta, and Bekasi City. Samples were taken as equipment swabs at the stall of poultry carcass vendors, tracheal swabs, and cloacal swabs of broiler chicken. The test used was Real-Time Reverse Transcription Polymerase Chain Reaction. Data analysis was performed using univariate, bivariate, and multivariate analysis with logistic regression. The prevalence of H9N2 virus contamination at the LBM level in Greater Jakarta in 2022 was 77.27% (95% CI, α 7%), while the prevalence at the vendor level was 54.02% (95% CI, α 5%). The risk factors for H9N2 virus contamination with a significant p-value<0.05 include the positive results on equipment swab samples, positive results on both equipment and broiler swabs, and the use of carcass sink.  The multivariate analysis model of H9N2 virus contamination at the vendor level was Logit P (H9N2=1 | x) = 0.29924 - 2.42176PPE + 1.58691carcass sink. The risk factor contributing to increase H9N2 contamination is using carcass sinks, while PPE is the one that reduces H9N2 contamination.
Co-Authors Aisah, Rahma Hani Bambang Sumiarto N.L.P.I. Mayasari, N.L.P.I. S. Setiyaningsih, S. Sodirun, Sodirun Wibawa, Hendra Widagdo Sri Nugroho