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All Journal Jurnal Ilmu Ternak Veteriner Tropical Animal Science Journal
H. D. Kusumaningrum, H. D.
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Agriculture, Biological Sciences & Forestry Energy Veterinary

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Antimicrobial and antioxidative activities of peptides from goat milk hydrolyzed with protease Kusumaningtyas, Eni; Widiastuti, Raphaella; Kusumaningrum, H. D.; Suhartono, M. T.
Indonesian Journal of Animal and Veterinary Sciences Vol 20, No 3 (2015): SEPTEMBER 2015
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.793 KB) | DOI: 10.14334/jitv.v20i3.1184

Abstract

Milk is highly nutritious food containing protein as a good source of bioactive peptide that beneficial for health. This research was aimed to explore potency of bioactive peptide derived from goat milk as an antimicrobial and antioxidant. Milk was hydrolyzed by trypsin, chymotrypsin, pepsin, or protease Bacillus sp. E.13. The peptides obtained were screened for antimicrobial activities through incubation with Staphylococcus aureus, Listeria monocytogenes, Salmonella thyphimurium and Escherichia coli at 106 CFU/mL at 37°C for two hours and plated on Mueller Hinton agar. Antimicrobial activities were determined by comparing the total bacterial colonies to that of bacterial control without peptides addition. Oxidative activity was determined by 2.2’-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and 2.2-diphenyl-1-picrylhydrazyl (DPPH) assays. Antimicrobial activities were shown in peptides produced from hydrolysis of goat milk protein by pepsin at 37°C, pH 2 for 90 min and by Bacillus sp. E.13 protease at 55°C, pH 11 for 30 and 60 min but the activities were not detected in peptides from hydrolysis by trypsin and chymotrypsin. Peptide from protein hydrolysis by Bacillus sp. E.13 protease could inhibit Listeria monocytogenes, Salmonella thyphimurium and Escherichia coli up to 5 log cycles. The antimicrobial peptides could scavenge ABTS radical up to 86 % and DPPH radical up to 9 % at 68 μg protein/mL. Results indicated that goat milk protein hydrolyzed by Bacillus sp. E.13 protease is potential as antimicrobes and antioxidant. Key Words: Goat Milk, Peptide, Antimicrobe, Antioxidant
Identification of Campylobacter jejuni from Chicken Carcasses and Characterization of Their Antibiotic Resistance Using Molecular Approach Susanty, S.; Dewanti-Hariyadi, R.; Kusumaningrum, H. D.
Tropical Animal Science Journal Vol. 48 No. 6 (2025): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2025.48.6.556

Abstract

Campylobacter species, particularly Campylobacter jejuni and Campylobacter coli, are foodborne pathogenic bacteria that cause campylobacteriosis, an acute gastroenteritis in humans. The most important transmission source to humans is contaminated animal products, such as chicken carcasses. Recently, there has been a growing concern about emerging antibiotic-resistant pathogens, including Campylobacter. Improper use of antibiotics in livestock can increase the risk of antibiotic resistance and the transmission of resistant bacterial strains to humans. This study aims to identify the species of Campylobacter spp. isolated from chicken carcasses and detected their antibiotic resistance genes using MinION sequencing in C. jejuni isolates. The disc diffusion method was also used to evaluate their resistance to macrolide, tetracycline, and fluoroquinolone antibiotics. The study results show that C. jejuni was identified in 64% of the 25 isolates, while C. coli was identified in 36% of the isolates. Molecular testing on C. jejuni isolates revealed that macrolide resistance genes were absent, specifically the 23S rRNA and the ermB genes. The tetO gene encoding for tetracycline resistance was detected in 62.5% of the isolates, and all 16 isolates (100%) were found to have the gyrA gene. In C. jejuni isolates, the cmeABC gene, which functions as a multidrug efflux pump, was also detected. The antibiotic resistance of C. jejuni isolates based on the disc diffusion method indicated high resistance to fluoroquinolone antibiotics, followed by tetracycline antibiotics.
Co-Authors Dewanti-Hariyadi, R. Eni Kusumaningtyas M. T. Suhartono Raphaella Widiastuti Susanty, S.