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All Journal Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan
Muhammad Chaniful Fuadi
Magister Program of Fisheries and Marine Biotechnology, Faculty of Fisheries and Marine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Energy Health Professions Immunology & microbiology Social Sciences Veterinary Other

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Application of the Cytochrome-b gene region in Population Study of Striped Snakehead (Channa striata) by Molecular Approach Sapto Andriyono; Muhammad Chaniful Fuadi; Nunuk Dyah Retno Lastuti
Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Vol 10, No 2 (2022): Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan
Publisher : UNIVERSITAS MUHAMMADIYAH PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29406/jr.v10i2.3915

Abstract

Snakehead fish with different geographical differences can have an impact on genetic diversity. DNA barcoding is a system designed to identify a species precisely and accurately by using short and standardized gene regions. This study aims to determine the diversity of snakehead fish populations in four areas in East Java. This study used the Cytochrome b (Cyt b) gene sequence in mitochondrial DNA to determine genetic variation in the wild population of Channa striata. The results of DNA barcodes with Cytochrome c oxidase subunit I (COI) showed that all samples were validly C. striata species (99.84-99.85%), while Cyt-b sequences had percentage identity with the same sequence species ranging from 99.74 to 100%. The phylogenetic tree reconstructed using MEGAX resulted in a separate clade in the sequences from East Java. This strengthens the similarity of the population of C. striata species in the Brantas watershed.
Application of the Cytochrome-b gene region in Population Study of Striped Snakehead (Channa striata) by Molecular Approach Sapto Andriyono; Muhammad Chaniful Fuadi; Nunuk Dyah Retno Lastuti
Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Vol 10, No 2 (2022): Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan
Publisher : UNIVERSITAS MUHAMMADIYAH PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (358.821 KB) | DOI: 10.29406/jr.v10i2.3915

Abstract

Snakehead fish with different geographical differences can have an impact on genetic diversity. DNA barcoding is a system designed to identify a species precisely and accurately by using short and standardized gene regions. This study aims to determine the diversity of snakehead fish populations in four areas in East Java. This study used the Cytochrome b (Cyt b) gene sequence in mitochondrial DNA to determine genetic variation in the wild population of Channa striata. The results of DNA barcodes with Cytochrome c oxidase subunit I (COI) showed that all samples were validly C. striata species (99.84-99.85%), while Cyt-b sequences had percentage identity with the same sequence species ranging from 99.74 to 100%. The phylogenetic tree reconstructed using MEGAX resulted in a separate clade in the sequences from East Java. This strengthens the similarity of the population of C. striata species in the Brantas watershed.
Co-Authors Nunuk Dyah Retno Lastuti Sapto Andriyono