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All Journal Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Biosains Pascasarjana Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Biotechnology Biosaintifika: Journal of Biology & Biology Education Jurnal Medik Veteriner Journal of Aquaculture Science Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Journal of Aquaculture and Fish Health Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Journal of Fisheries & Marine

Nunuk Dyah Retno Lastuti

Departemen Parasitologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga Kampus C Unair, Jl. Mulyorejo, Kota Surabaya, Jawa Timur, Indonesia 60116

Author-ID : 676897

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Energy Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Study on the Morphology of Fasciola gigantica and Economic Losses due to Fasciolosis in Berau, East Kalimantan Prasetya, Muhammad Rofi'; Koesdarto, Setiawan; Lastuti, Nunuk Dyah Retno; Suwanti, Lucia Tri; Kusnoto, Kusnoto; Yunus, Muchammad
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Fasciolosis is one of the endemic parasitic diseases in Indonesia and causes significant economic losses. Fasciola gigantica is the main species found to infect livestock. This research aimed to describe the morphology of F. gigantica histologically using hematoxylin and eosin staining as well as ultrastructure morphology using scanning electron microscopy (SEM). Besides, the study was also carried out to determine the prevalence and estimation of economic losses due to fasciolosis in Berau Regency. The histological identification of flukes morphology found several organs including oral sucker (OS), ventral sucker (VS), cirrus (C), genital pore (GP), uterus (U), ovary (O), tegument (Te), testis (T), and caecum (C). In this study, ultrastructure morphology was divided into anterior and midbody region. In the anterior region, there are an oral sucker (OS) with a diameter of 304.5 µm and a ventral sucker (VS) with a diameter of 571 µm. It was also found the anterior spine with a width of 25.4 µm and a height of 22.8 µm. In the midbody, the spine has a width of ± 26.7 µm and a length of ± 38.9 µm leading posteriorly. Postmortem examination results showed that the prevalence of fasciolosis in Berau regency was 21.65% or 34 of 157 cows which were positive with an estimated loss of 228,656,475.00 IDR/16,450 USD (1 USD=13,900 IDR). The amount of loss due to fasciolosis must be a particular concern in carrying out control and prevention measures. Histology and ultrastructure morphology can become a reference in the development of anthelmintic testing that has been used so far in Indonesia.

Performance of CO1 and ITS2 nested PCR in molecular identification of ordinary scabies (Sarcoptes scabiei var. hominis) Gita Dwi Prasasty; Miftahurrizqiyah Miftahurrizqiyah; Chairil Anwar; Dwi Handayani; Dalilah Dalilah; Ahmad Ghiffari; Inda Astri Aryani; Nunuk Dyah Retno Lastuti; Afiat Berbudi
Indonesian Journal of Biotechnology Vol 26, No 4 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.64472

Scabies is a global disease with a high prevalence, causing morbidity and even mortality, especially in poor and developing countries. However, it is often misdiagnosed due to varied and unspecified lesions. The gold standard technique for diagnosis is a microscopic examination, which requires experienced experts in finding mites, mainly in ordinary scabies. CO1 and ITS2 genes have been widely used in molecular identification to detect Sarcoptes scabiei and its variants. This study aimed to determine and compare the sensitivity and specificity of CO1 and ITS2 S. scabiei genes to the microscopic examination of scabies skin scrapings. The skin scrapings of 52 subjects with scabies diagnosed by anamnesis, physical examination, and dermoscopic examination were examined under a microscope and analyzed by nested PCR. The diagnostic test result showed that the sensitivity of nested PCR of both CO1 and ITS2 genes to micro‐ scope examination was 100%. However, the specificity of both CO1 and ITS2 nested PCR was poor (24% and 0%). Hence, CO1 and ITS2 nested PCR could be more suitable for screening ordinary scabies in humans than the microscopic examination.

Prevalence and Saverity Level of Scabies (Sarcoptes scabiei) on Rabbits in Sajen Village, Pacet SUB-District, Mojokerto Regency Taufik Tri Laksono; Gandul Atik Yuliani; Agus Sunarso; Nunuk Dyah Retno Lastuti; Lucia Tri Suwanti
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Penelitian ini bertujuan untuk mengetahui prevalensi dan tingkat keparahan scabies (Sarcoptes scabiei) pada ternak kelinci di Desa Sajen, Kecamatan Pacet, Kabupaten Mojokereto. Sarcoptes scabiei var. cuniculi merupakan tungau dengan predileksi di stratum korneum dari kulit dan penyebab scabies. Gejala klinis ditandai dengan alopecia and krusta pada kulit. Penelitian ini menggunakan metode penelitian eksploratif labolatorik dengan pendekatan cross sectional. Sampel yang digunakan adalah kelinci pada satu peternakan yang memiliki gejala klinis scabies dan hasil dari kerokan kulit pada pemeriksaan laboratoris secara natif. Kelinci yang positif scabies kemudian dilakukan skoring berdasarkan tingkat keparahan pada regio tubuh dan gejala klinisnya. Hasil penelitian menunjukkan bahwa dari 110 ekor kelinci dalam populasi, terdapat 19 ekor yang terinfeksi scabies dan terdapat 2 tingkat keparahan scabies pada kelinci yaitu tingkat keparahan ringan dan sedang yang terbagi atas 2 regio tubuh. Tingkat keparahan ringan menunjukkan gejala klinis alopecia dan krusta pada regio wajah sedangkan tingkat keparahan sedang pada regio wajah dan kaki. Kesimpulan pada penelitian ini adalah angka prevalensi scabies sebesar 17,27% dan tingkat keparahan ringan sebesar 26,32% serta tingkat keparahan sedang sebesar 73,68.

The Conformity of Diagnostic Test Between Burrow Ink Test Method With Skin Scrapping Method of Scabies in Rabbit (Lepus domesticus) Fadila Zikri Amanda; Poedji Hastutiek; Emy Koestanti Sabdoningrum; Nunuk Dyah Retno Lastuti; Endang Suprihati; Hana Eliyani
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

This study intend to know the conformity of diagnostic test between burrow ink test method with skin scrapping method of scabies in rabbit. Scabies caused by Sarcoptes scabiei which has a predilection in stratum corneum and lucidum. Clinical symptoms was characterized by papula or crusting of the skin. This study presents a laboratory explorative research with cross sectional approach. Samples used 20 rabbits that infested scabies naturally. Data obtained by performing diagnostic burrow ink test method and skin scrapping in rabbits which are infested scabies naturally. KAPPA test used as data analysis. Results showed that the number of positive results based on burrow ink test method are 12 rabbits and 8 rabbits are negative while skin scrapping obtained the positive results are 11 rabbits and 9 rabbits are negative. KAPPA test analysis performed on the overall study data was obtained 0,490. The conclusion of this study is burrow ink test method has a good conformity with the skin scrapping in rabbits which are infested scabies naturally that can be used for diagnostic analysis of scabies with a simple method, faster, and have the same ability with skin scrapping without hurting animals.

Identification of Worm In Digestive Tract of Consumed Cattle Egret (Bubulcus ibis) In Ujung Pangkah, Gresik Nisa’ Rachmaningtyas Putri; E Djoko Poetranto; Nunuk Dyah Retno Lastuti; Setiawan Koesdarto; Bambang Sektiari Lukiswanto; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Ujung Pangkah, Gresik was became the most habitat of fowls such as Cattle egret (Bubulcus ibis). The aim of this research were to find out the type of worm that infected digestive tract of Cattle egret (Bubulcus ibis) in Ujung Pangkah, Gresik. The examination of worm in digestive tract was done by dissection and fecal examination. The identification based on morphological of the eggs and worms. The samples of digestive tract organ collected from restaurant that preserve Cattle egret as a main menu. Whereas the fecal sample collected by swab from the intestine. Cattle egret (Bubulcus ibis) has positive infection by worm parasite if there were worm or egg inside the digestive tract. Fecal samples were examined by native, sedimentation, coconut-sugar floating method and EPG counting with Lucient Brumpt. And the worm morphology from dissection of digestive tract would be identify with source book, journal, and discussed with supervisor. The result showed that 20 of digestive tract dissection and 20 fecal samples, there were three types of worm, Contracaecum sp., Cosmocephalus sp., Capillaria sp. and egg of Contracaecum sp. with 200 EPG

Detection of Blood Protozoa Infecting Broiler Chicken Farms in Tanjung Gunung Village, District Jombang Marchelia Arifiandani; Endang Suprihati; Wiwik Misaco Yuniarti; Nunuk Dyah Retno Lastuti; Poedji Hastutiek; Sunaryo Hadi Warsito
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

Broiler chicken farms could be a part of potential source infection Leucocytozoonosis and Plasmodiosis disease due to the death of livestock. Broiler farms in Tanjung Gunung, District Jombang near the fields, water, and trees. This could be increase the population of vectors infection Leucocytozoonosis and Plasmodiosis. The main purpose of this researchis to detect Leucocytozoonosis and Plasmodiosis infections on broiler farms in Tnjung Gunung Village, District Jombang using 50 broiler chickens by Purposive Sampling of 2 bredeers. The result show rhat there are 6 positive samples infected Leucocytozoonosis, while Plasmodiosis infection could not be fond. Need cage sanitation to break life cycles of vector Leucocytozoonosis and Plasmodiosis.

The Prevalence and Infection’s Degree of Gastrointestinal Worm of Local Chicken (Gallus domesticus) in Kramat Village, District of Bangkalan, Madura, East Java Indonesia Ellza Agatha Damayanti; Poedji Hastutiek; Agnes Theresia Soelih Estoepangestie; Nunuk Dyah Retno Lastuti; Kusnoto Kusnoto; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The study was aimed to know the prevalence and level of gastrointestinal worm investation of local chicken (Gallus domesticus) in Kramat Village, district of Bangkalan, Madura East Java province. The samples used in the study were 100 faeces of kampung chicken. using sedimentation and floating method. Faeces samples were tested, than followed by microscopically test to identify the worm is species. The result showed that in 38 out of 100 samples could be indentified some worm species as followed : Capillaria sp.14 (36,8%), Railletina 7 (18,4%), Hymenolepis 16 (42,1%) and Heterakis gallinarum 1 (2,6%). Using Mann Whitney U-test could be differentiated the level of the infection with an average of 45.53 in cock, while in hen with an average of 55.47. There was no significancy (p>0,05) on the level of worm gastrointestinal infection of different sex of kampung chicken.

Identification of Gastrointestinal Endoparasite in Bawean Deer (Axis kuhlii) and Spotted Deer (Axis axis) at Bratang Flora Park – Surabaya Hartono Hartono; Endang Suprihati; Erma Safitri; Nunuk Dyah Retno Lastuti; Mufasirin Mufasirin; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this study is to identify the endoparasites of Spotted Bawean Deer (Axis kuhlii) and Deer (Axis axis ) at Surabaya Flora Park. The research was conducted in December 2018 - January 2019 used total 60 specimen of sample faeces, consist of 30 sample of bawean deers faeces and 30 sample of spoted deers faeces, the samples analyzed at the Department of Parasitology Veterinary, Faculty of Veterinary Medicine, Universitas Airlangga. The results of the study found positive endoparasites on examination, samples of Bawean deer faeces (Axis kuhlii) and Spotted deer faeces(Axis axis) in Baratang Flora Park Surabaya found that 26 positive samples from 60 samples were obtained. Positive endoparasitic samples are protozoa at the stage of oocyst, namely Eimeria sp. 6 (10%) samples, worm eggs from the Nematoda class, Haemonchus sp. 20 (33.33%) and whereas endoparasites from other stages and classes were not found. The results of the analysis used Chi-Square test and showed no significantly differences degree of gastrointestinal endoparasite infection of Bawean deer (Axis kuhlii) and Spotted deer (Axis axis) (p>0.05).

The Potential of Anting – Anting (Acalypha indica L.) Leaf Extract as Anti-Scabies to Sarcoptes scabiei var. Cuniculi in vitro Luluk Tri Astuti; Rahmi Sugihartuti; Lianny Nagoi; Nunuk Dyah Retno Lastuti; Dewa Ketut Meles; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The Aim of research to determine the potential of anting-anting (Acalypha indica L.) leaf extract as anti-scabies to Sarcoptes scabiei var. Cuniculi in vitro by observing the mortality rates and lethal concentration of Sarcoptes scabiei. There were 5 treatments namely DMSO 0.5% (PN). Amitraz (P0), anting-anting (Acalypha indica L.) leaf extract concentratrion of 6,25% (P1), 12,5% (P2) and 25% (P3). Observations were carried out for 8 hours with stereomicroscope. The data was analyzed using rpobit analysis with SPSS 20.0 software. The results showed that the fastest mortality rate of S. Scabiei was group p3 at 4.6 mites/hour., then P0, P2, P1 and PN respectively 3.5, 3.1, 1.9, and 1.3 mites/ hour. The smallest concentration of anting-anting (Acalypha indica L.) leaf extract was not significantly different compared to amitraz for killing of 50% and 90% S. Scabiei was 12.5% with lethal time (LT50) 1.82 hours and (LT90) 3.69 hours. In conclusion, anting-anting (Acalypha indica L.) leaf extract has mortality rate against S. Scabiei var. Cuniculi of 3.1 mites/hour, lethal concentration of 12.5% and potential as anti-scabies in vitro.

Gambaran Jumlah dan Hitung Jenis Leukosit Ayam Petelur yang diinfeksi L2 Toxocara Cati Diyah Ayu Candra; Nunuk Dyah Retno Lastuti; Nove Hidayati; Kusnoto Kusnoto; Poedji Hastutiek; Retno Bijanti
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this study was to determine leukocytes and differential counting in chicken after infected with L2 Toxocra cati. In this study, it was used twenty chickens, wich were 14 weeks old. They were divided into 4 groups. Chickens were infected by orally with dose 0 eggs/ml L2 T. cati, 10 eggs/ml L2 T. cati, 100 eggs/ml L2 T. cati and 1000 eggs/ml L2 T. cati. Blood sampling were conducted on 2, 7 and 21 days after infection. Leukocyte value was determined by improve neubauer and differential counting stained with Wright’s stain then determined using microscope 1000x. The data was analyzed by Anova Factorial and then continued by BNJ Test at 5%. The result showed that increased of leukocytes value and eosinophil at 7 and 21 days after infection L2 T. cati with different dose, increased of leukocytes value and eosinophil on infection dose 10 eggs/ml L2 T. cati, 100 eggs/ml L2 T. cati and 1000 eggs/ml L2 T. cati. It shows that there was not intereraction between time of infection process and infection dose of L2 T. Cati.

Co-Authors Abdullah Abdullah Adamu Ayubu Mwendolwa Adamu Ayubu Mwendolwa Afiat Berbudi Agnes Theresia Soelih Estoepangestie Agustina, Firanda Ahmad Ghiffari Aldi Hamdani Amalia Rosydinasari Rosydinasari Amirotul Azhimah Amirul Muslim Amrullah Anam Al Arif Anord Charles Nkuba Bambang Sektiari Lukiswanto Chairil Anwar Chaniful Fuadi, Muhammad Dadik Rahardjo, Dadik Dadik Raharjo Damayanti, Tita Desiandura, Kurnia Dian Ayu Permatasari Didik Handijatno Diyah Ayu Candra Doohan Mahendra Dwi Ananda, Novita Dwi Handayani Dyah Ayu Kurniawati E Djoko Poetranto Ellza Agatha Damayanti Emy Koestanti Sabdoningrum Endang Suprihati Erma Safitri Fadila Zikri Amanda Fatmawati, Mira Fatmawati Fedik Abdul Rantam Fransiska Cicilia Beka Gandul Atik Yuliani Gita Dwi Prasasty Gunanti Mahasri Hana Eliyani Hartono Hartono Hidajanti, Nove Inda Astri Aryani Ira Sari Yudaniayanti Kadek Rachmawati Koesdarto Koesdarto Kurniawati, Dyah Ayu Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lianny Nagoi Lilik Maslachah Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Luluk Tri Astuti Lutfiah Annisa Billa Marchelia Arifiandani Masdiana C Padaga Maudika Pamela Fiorenza Meles, Dewa Ketut Meyreta Doti Alcaterana Miftahurrizqiyah Miftahurrizqiyah Mirni Lamid Mochamad Lazuardi Mochammad Amin Alamsjah Moh. Ilham Rizkul Ulum Muchammad Yunus Mufasirin Muhamad Amin Muhammad Athoillah Sholahuddin Muhammad Chaniful Fuadi Muhammad Fahmi Abdillah Muhammad Rofi' Prasetya Mustofa Helmi Effendi Ni Komang Aprilina Widisuputri Nisa’ Rachmaningtyas Putri Nove Hidayati Nusdianto Triakoso P Kumaladewi Pinatih, Ayu Komang Ria Trie Dewi Poedji Hastutiek Prasetya, Muhammad Rofi' Pratiwi, Arif Prawasa, Ramadhiniyanti Putri Alif Rahmi Sugihartuti Retno Bijanti Rizhar Eman Karunia Akbar Rochmah Kurnijasanti Romy Muhammad Dary Mufa Sapto Andriyono Setiawan Koesdarto Soeharsono Soeharsono Sri Agus Sudjarwo Sri Subekti Sunarso, Agus Suzanita Utama Taufik Tri Laksono Tjuk Imam Restiadi Trifena Pristi Anindyta Veena Vasheene Vijaya Kumar Vindo Rossy Pertiwi Warsito, Sunaryo Hadi Widi Nugroho Widiyatno, Thomas Valentinus Win Darmanto Wiwik Misaco Yuniarti Wurlina, W Yeni Dhamayanti Yunus, Muchammad Yunus, Muchammad

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