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Hamsidar Hasan
Jurusan Farmasi Fakultas Olahraga dan Kesehatan, Universitas Negeri Gorontalo, Jl. Jenderal Sudirman No. 06 Kota Gorontalo 96128, Indonesia
Chemistry Medicine & Pharmacology

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Uji Aktivitas Antidiabetes Ekstrak Etil Asetat Daun Lamun (Enhalus acoroides) Pada Mencit (Mus musculus) Hamsidar Hasan; A.Mu'thi Andy Suryadi; Zulfianto Djufri
Journal Syifa Sciences and Clinical Research Vol 4, No 1 (2022): Volume 4 Edisi 1 2022
Publisher : State University of Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/jsscr.v4i1.15379

Abstract

Diabetes mellitus is a severe condition, chronic in nature, that occurs when there is an increase in blood glucose levels (GDS) 200 mg/dL or hyperglycemic conditions. Some plants are reported to have anti-diabetic effects. One of the examples is seagrass (Enhalus acoroides), which has α-glucosidase enzyme inhibitory effects in vitro. The community often uses it as traditional medicine. This present work explores the anti-diabetic activity of ethyl acetate extract of seagrass (Enhalus acoroides) leaves against male mice (Mus musculus) charged with 90% sucrose solution. It employed the oral sucrose tolerance test (OSTT) methodology. Activity tests on mice were carried out in five treatment groups, namely: group I or a negative control (Na-CMC 1% w/v); group II or a positive control (acarbose); group III (ethyl acetate extract dose 50 mg/kg BW); group IV ( ethyl acetate extract at a dose of 150 mg/kg BW), and; group V (ethyl acetate extract at a dose of 250 mg/kg BW). All data were analyzed by using a one-way ANOVA statistical test. The results show that the ethyl acetate extract of seagrass leaves can inhibit the absorption of sucrose, in which the most significant total inhibition occurred at a dose of 250 mg/kg BW, i.e., 68 mg/dL. A substantial inhibition was also found at a dose of 150 mg/kg BW, which was 82 mg/dL and at a dose of 50 mg/dL or 103 mg/dL. In conclusion, the ethyl acetate extract of seagrass leaves has anti-diabetic activity.
Senyawa Antidiabetes Fraksi Aktif Daun Ketapang (Terminalia catappa L.) Hamsidar Hasan; Endah Nurrohwinta Djuwarno; Hasrita Samudi; Widy Susanti Abdulkadir; Faramita Hiola
Journal Syifa Sciences and Clinical Research Vol 4, No 2 (2022): Volume 4 Edisi 2 2022
Publisher : State University of Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/jsscr.v4i2.15656

Abstract

Antidiabetic is a compound that has a vital role in treating diabetes mellitus. Diabetes mellitus is a chronic metabolic disorder that is characterized by an increase in blood sugar levels. The use of traditional medicine is relatively cheaper and safer compared to synthetic drugs. One of the plants that is often used in traditional herbal medicine is ketapang (Terminalia catappa L.) because it contains a secondary metabolite compound that acts as an antidiabetic. Thus, this present study aimed to determine the n-hexane, ethyl acetate, and methanol extracts that had antidiabetic efficacy on blood sugar levels and compound group that acts as antidiabetic. The research employed mehods of multiple maceration, phytochemical screening, thin layer chromatography, and antidiabetic test, while the most effective antidiabetic efficacy was then continued to the fractionation stage using vacuum liquid chromatography and gravity column chromatography. Furthermore, the preparative thin layer chromatography (PTLC) analysi, UV-Vis zspectrometry and FTIR were carried out. The research findings revealed that the maximum wavelength of active fraction in ketapang (Terminalia catappa L.) leaves was 410.50 nm, and FTIR spectrum had functional groups of O-H (hydroxyl) C=O (carbonyl Co (aliphatic), C-O (ether) and CH3. Based on the finding, it could be concluded, that antidiabetic compound that plays a role in active fraction of ketapang (Terminalia Cappy L.) leaves was a flavonoid compound.
Isolasi dan Uji Aktivitas Antiinflamasi Ekstrak Daun Andong (Cordyline fructicosa (L) A.Chev) Endah Nurrohwinta Djuwarno; Hamsidar Hasan; Mahdalena Sy. Pakaya; Faramita Hiola; Dewa Ayu Puspita
Journal Syifa Sciences and Clinical Research Vol 4, No 3 (2022): Volume 4 Edisi 3 2022
Publisher : State University of Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/jsscr.v4i3.15740

Abstract

Andong (Cordyline fructicosa (L) A. Chev) is used as traditional medicine, the use of this traditional medicine is related to the content of secondary metabolites contained in the andong plant. Empirically people use this plant as an antioxidant, the compound that acts as an antoixidant is related to antiinflamasi. The purpose of this study was to isolate secondary metabolites from the extracts of N-Hexane, Ethyl Acetate, and Methanol from Andong (Cordyline fructicosa (L) A. Chev) leaves and to determine the anti-inflamatory effect of the isolated secondary metabolites of Andong (Cordyline fructicosa (L) A. Chev) leaves through in vitro with red blood cell stabilization method. Several methods are used in the isolation of these compounds, namely extraction, identification of compound, fractionation of KCV and KKG, KLT,KLTP, and characterizationof compounds using UV-VIS spectrophotometry and FTIR. The results of UV-VIS characterization show a maximum wavelength of 434 nm, and the results of  FTIR characterization indicate the presence of a functional groups namely O-H (Hidroxyl) at a wave number of 3448,72 cm-1, C-H (Aliphatic) at a wave number of 2954,95 cm-1, 2920,23 cm-1, and 2852,72 cm-1, aliphatic compounds are supported by the presence of CH2 and CH3 at wave number of 1463,97 cm-1 and 1379,10 cm-1, C=O (conjugated carbonyl) at wave number of 1721,79 cm-1, C=C at wave number of 1633,71 cm-1, C-O at wave number of 1294,24 cm-1. So it is very likely that the compounds in Andong leaves isolate are flavonoid compounds. Furthermore, the activity of results of the isolation is tested where the isolate whit a concentration of 100 ppm signifies the highest anti-inflamatory activity, with the ability to stabilize red blood cell membranes of 80,142 %.
Penentuan Kadar Flavonoid Daun Rumput Knop (Hyptis capitata Jacq.) Menggunakan Spektrofotometri UV-Vis Hamsidar Hasan; A. Mu'thi Andy Suryadi; Syamsul Bahri; Ni Luh Widiastuti
Journal Syifa Sciences and Clinical Research Vol 5, No 2 (2023): Volume 5 Edisi 2 2023
Publisher : State University of Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/jsscr.v5i2.19371

Abstract

Knop grass (Hyptis capitata Jacq.) is a weed used as a tradisional medicine, especially for the leaves. Flavonoids are one of the secondary metabolites contained in the Knop Grass (Hyptis capitata Jacq.) leaves, wich function as anti-inflammatory, analgesic, antipyretic and anti-diabetic. The amount of extracted flavonoids is optimized by choosing the right extraction method, as it will determine the amount of substance that can be attracted and obtain a high content of active substansces. This research aimed to compare the flavonoid levels in the methanol extract of Knop Grass (Hyptis capitata Jacq.) leaves in two different maceration methods: multilevel maceration and total maceration. Multilevel maceration employed n-hekxane, ethyl acetat and methanol solvents, while total maceration employed methanol solvent. In the meantime, the qualitative analysis in this reaserch used the color reagent test method, whereas the thin layer chromatography used the eluent, namely n-hexane : ethyl acetate with a ratio (7:3). In addition, the quantitative analysis used the UV-Vis spectrophotometry method at a maximum wavelength of 435 nm with standard quercetin as a comparison. The result signified that the average flavonoid level in the methanol extract of Knop Grass (Hyptis capitata Jacq.) leaves using the multilevel maceration method was 2,9750%. The total maceration method was 4,8822%. Furthermore, the total maceration method donated the highest concentration obtained from the methanol extract of Knop Grass (Hyptis capitata Jacq.) leaves.
Co-Authors A. Mu'thi Andy Suryadi A.Mu'thi Andy Suryadi Dewa Ayu Puspita Endah Nurrohwinta Djuwarno Hasrita Samudi Hiola, Faramita Mahdalena Sy Pakaya Ni Luh Widiastuti Syamsul Bahri Widy Susanti Abdulkadir Zulfianto Djufri