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All Journal BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) JMM (Jurnal Masyarakat Mandiri) Pharmasipha Jambura Journal of Health Sciences and Research Jambura Journal of Chemistry Journal Syifa Sciences and Clinical Research (JSSCR) Jurnal Sibermas (Sinergi Pemberdayaan Masyarakat) Indonesian Journal of Pharmaceutical Education Indonesian Community Journal Jurnal Pengabdian Masyarakat Farmasi : Pharmacare Society Jurnal Farmasi Teknologi Sediaan dan Kosmetika (JFTSK) Journal of Community and Clinical Pharmacy Journal of Pharmacology and Natural Products
Mahdalena Sy Pakaya
UNIVERSITAS NEGERI GORONTALO
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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UJI EFEKTIVITAS SEDIAAN PATCH EKSTRAK DAUN CENGKEH (Syzygium aromaticum) SEBAGAI REPELLENT NYAMUK Sy. Pakaya, M.Si.,Apt, Mahdalena; Thomas, Nurain; Arista Idris, Pinkan
Journal Syifa Sciences and Clinical Research Vol 1, No 1 (2019): March
Publisher : State University of Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Clove (Syzygium aromaticumL.) leaf has efficacy as repellent because it contains terpenoid eugenol. This study aimed to determine how the effectiveness of clove leaf extract patch dosage as a mosquito repellent. This research was started with extraction using maceration method with 96% ethanol solvent. Furthermore, making the dosage of patch of clove leaf extract using matrix method with variation of the clove leaf extract concentration. Then, the effectiveness of mosquito repellent patch was tested by using rabbit that divided into 5 treatment groups namely K- (base patch), K+ (mosquito repellent patch), 3 groups of clove leaf extract patch with variation of concentration of F1a (concentration of 1%), F1b (concentration of 3%), and F1c (concentration of 5%). Each group was attached to the clothes of the rabbit and put into the experimental box that had been put by mosquito as many as 20 tails. Based on result of ANNOVA statistic test showed that clove (Syzygium aromaticumL.) leaf patch dosage against the rabbit gave a significant effect against mosquito repellent, where at 5% concentration of clove leaf extract could give mosquito repellent efficacy better than concentration 1% and 3%.
UJI EFEKTIVITAS SEDIAAN PATCH EKSTRAK DAUN CENGKEH (Syzygium aromaticum) SEBAGAI REPELLENT NYAMUK Sy. Pakaya, M.Si.,Apt, Mahdalena; Thomas, Nurain; Arista Idris, Pinkan
Journal Syifa Sciences and Clinical Research Vol 1, No 1 (2019): March
Publisher : State University of Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (473.51 KB)

Abstract

Clove (Syzygium aromaticumL.) leaf has efficacy as repellent because it contains terpenoid eugenol. This study aimed to determine how the effectiveness of clove leaf extract patch dosage as a mosquito repellent. This research was started with extraction using maceration method with 96% ethanol solvent. Furthermore, making the dosage of patch of clove leaf extract using matrix method with variation of the clove leaf extract concentration. Then, the effectiveness of mosquito repellent patch was tested by using rabbit that divided into 5 treatment groups namely K- (base patch), K+ (mosquito repellent patch), 3 groups of clove leaf extract patch with variation of concentration of F1a (concentration of 1%), F1b (concentration of 3%), and F1c (concentration of 5%). Each group was attached to the clothes of the rabbit and put into the experimental box that had been put by mosquito as many as 20 tails. Based on result of ANNOVA statistic test showed that clove (Syzygium aromaticumL.) leaf patch dosage against the rabbit gave a significant effect against mosquito repellent, where at 5% concentration of clove leaf extract could give mosquito repellent efficacy better than concentration 1% and 3%.
UJI POTENSI ANTIBAKTERI EKSTRAK BATANG GAHARU (Gyrinops versteegii) TERHADAP BAKTERI Escherichia coli dan Staphylococcus aureus Sy. Pakaya, Mahdalena; Mustapa, Moh. Adam; Ali, Muthiah Rahmah
Indonesian Journal of Pharmaceutical Education Vol 1, No 3 (2021): September 2021
Publisher : Jurusan Farmasi Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/ijpe.v1i3.11417

Abstract

Penyakit infeksi merupakan salah satu masalah kesehatan yang paling utama di Indonesia maupun di negara-negara berkembang lainnya. Kontaminasi mikroba berkaitan dengan penyebab infeksi tersebut. Infeksi dapat disebabkan oleh berbagai mikroba seperti bakteri, virus, jamur, dan protozoa. Salah satu tumbuhan berkhasiat obat yang berada di Indonesia adalah gaharu (Gyrinops versteegii). Penelitian ini bertujuan untuk mengukur potensi ekstrak batang gaharu (Gyrinops versteegii) terhadap bakteri Escherichia coli dan Staphylococcus aureus. Dimana simplisia batang gaharu diekstraksi menggunakan metode maserasi bertingkat dengan pelarut n-Heksan, etil asetat dan metanol. Hasil uji aktivitas antibakteri terhadap 3 ekstrak tersebut yaitu ekstrak etil asetat mampu menghambat pertumbuhan Escherichia coli dan Staphylococcus aureus. Hasil skrining ekstrak etil asetat mengandung alkaloid, terpenoid dan tanin. Kontrol positif yang digunakan yaitu kloramfenikol dan kontrol negatif yaitu Dimetil Sulfoksida (DMSO). Hasil Uji KHM ekstrak batang gaharu terhadap Escherichia coli dan Staphylococcus aureus yaitu pada konsentrasi 50% ditandai dengan tidak terjadi kekeruhan dan 50% untuk nilai KBM dimana tidak terjadi pertumbuhan bakteri pada media Nutrien Agar (NA). Hasil Uji Potensi ekstrak etil asetat batang gaharu terhadap Escherichia coli pada konsentrasi 25%, 50%, dan 100 % dengan zona hambat yang dihasilkan yaitu 7,85 mm dimana masuk dalam kategori sedang, 10,6 mm dan 13,45 mm dimana masuk dalam kategori kuat. Sedangkan uji potensi ekstrak etil asetat batang gaharu terhadap Staphylococcus aureus pada konsentrasi 25%, 50%, dan 100 % dengan zona hambat yang dihasilkan yaitu 8,1 mm dimana masuk dalam kategori sedang, 13,2 mm dan 16 mm dimana masuk dalam kategori kuat dalam menghambat bakteri. Infection disease is one of the most common health problem in many developing countries, including Indonesia where the contamination of microbe is associated with the cause of the infection. The infection can be caused by various microorganisms such as bacteria, virus, fungus, and protozoa. Fortunately, Indonesia’s medicinal plant varies and one of which is agarwood (Gyrinops versteegii). This study aims at measuring the potential of agarwood (Gyrinops versteegii) stem extract towards Escherichia coli and Staphylococcus aureus. In this study, the simplicial of agarwood stem is extracted by applying multilevel maceration method with n-Hexane solvent, ethyl acetate, and methanol. The result of antibacterial activity testing over three extracts reveals that the ethyl acetate extract can inhibit the growth of Escherichia coli dan Staphylococcus aureus. In addition, the result of screening in the ethyl acetate extract discovers that in contains alkaloid, terpenoid, and tannin, where the positive control used is chloramphenicol and the negative control is Dimethyl Sulfoxide (DMSO). The result of Minimum Inhibitory Concentration test in the agarwood stem extract towards Escherichia coli dan  Staphylococcus aureus shows that the concentration of 50% is marked with absence of turbidity, whereas 50% in the Lowest Lethal Dose value signifies absence of growth in the bacteria in the Nutrient Agar (NA) media. Additionally, the result of potential test of agarwood stem ethyl acetate extract towards Escerichia coli in concentrations of 25%, 50%, and 100% with an inhibition zone created of 7,85 mm where it includes in medium category, and 10,6 mm and 13,45 mm that include in strong category. In the meantime, the result of potential test of agarwood stem ethyl acetate extract towards Staphylococcus aureus in concentrations of 25%, 50%, and 100% with an inhibition zone created of b,1 mm where it includes in medium category, and 13,2 mm and 16 mm that include in strong category in inhibiting bacteria.
UJI TOKSISITAS EKSTRAK DAUN KENCUR (Kaempferia galanga L) DENGAN METODE BRINE SHRIMP LETHALY TEST (BSLT) sy pakaya, mahdalena; hasan, hamsidar; umar, linsih
Indonesian Journal of Pharmaceutical Education Vol 2, No 1 (2022): Januari-April 2022 (in press)
Publisher : Jurusan Farmasi Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/ijpe.v2i1.11283

Abstract

One of the plants that has potential as medicine is kencur(Kaemoferia galanga L). Many studies have asserted that the rhizome of kencur is useful for bacterial infections, cough medicine, dysentery, cold, and stomachache. Therefore, the research aimed to identify the toxic effect of kencur leaf extract on artemia salina larvae applying the BSLT (Brine shrimp lethaly test) method. It employed three concentration treatments using 10 artemia salina larvae that died after 48 hours. Futher, it was calculated by LC50with probit analysis. The result of probit analysis revealed that the value of LC50from n-hexane extract was 96,13µg/mL, ethyl acetate extract was 98,22 µg/mL and methanol extract of kencur leaf was 156,06 µg/mL. The LC50 value of the extract was in the active category, with the value of LC50was lower than 1000 ppm.
Pemberdayaan Masyarakat Kabupaten Boalemo Melalui Pelatihan Dan Pembuatan Produk Sediaan Herbal Dari Tanaman Obat Dalam Rangka Pencapaian Sy. Pakaya, Mahdalena; Thomas, Nur Ain
Jurnal Sibermas (Sinergi Pemberdayaan Masyarakat) Vol 11, No 1 (2022): Jurnal Sibermas (Sinergi Bersama Masyarakat)
Publisher : Univeristas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/sibermas.v11i1.12043

Abstract

Indonesia memilki potensi sebagai sentra penghasil obat tradisional. Biodiversiti tumbuhan yang melimpah, menjadi pendukung utama untuk dikembangkannya produk obat herbal. Sehingga bisa menjadi lahan pembuka lapangan pekerjaan baru di masyrakat.  Oleh karena itu diperlukan adanya berbagai program pembinaan dengan memanfaatkan dan mengelola tumbuhan maupun tanaman obat yang ditanam disekitar pekarangan rumah untuk dioah sebaga obat tradisional. Tujuan dari pengabdian ini adalah meningkatkan pendapatan masyarakat  dengan memberikan pelatihan pembuatan produk herbal. Adapun delapan produk yang dipraktikan secara langsung yakni  Serbuk Jahe Instan, Serbuk Kunyit Asam Instan, Kapsul Jahe/Kunyit/Temulawak/Sereh, Susu Jagung Jahe, Susu Rempah, Lemonggrass Tea, Teh Herbal, Permen Jahe dan Minyak Balur. Metode yang digunakan adalah ceramah dan praktik atau demonstrasi. Sehingga masyarakat dapat mempraktikkan secara langsung dan menerapkan dalam  rumah tangga.
STANDARISASI DAN KADAR FLAVONOID TOTAL EKSTRAK ETIL ASETAT KULIT BATANG NANGKA (Artocapus heterophylus L) Niluh Sri Purnama; Hamsidar Hasan; Mahdalena Sy Pakaya
Indonesian Journal of Pharmaceutical Education Vol 1, No 3 (2021): September-Desember 2021
Publisher : Jurusan Farmasi Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/ijpe.v1i3.11140

Abstract

Nangka (Artocarpus heterophylla L) adalah tumbuhan yang banyak terdapat di Indonesia. Hampir seluruh bagian pohon nangka dapat dimanfaatkan sebagai obat herbal dimana senyawa flavonoid terprenilasi merupakan metabolit sekunder utama yang terdapat dalam genus Artocarpus. Di Indonesia penggunaan obat herbal masih bersifat tidak terukur baik dari segi takaran, maupun proses penyiapannya. Sehingga perlu dilakukan standarisasi hal ini dilakukan untuk menjaga konsistensi serta keseragaman dari bahan obat herbal. Penelitian ini bertujuan untuk menentukan parameter standarisasi spesifik dan non spesifik serta menentukan kadar flavonoid total ekstrak etil asetat kulit batang nangka. Metode ekstraksi yang digunakan adalah maserasi bertingkat menggunakan pelarut n-heksan, etil asetat dan metanol. Hasil penelitian mengenai parameter organoleptik dari ekstrak etil asetat kulit batang nangka yaitu berwarna merah kehitaman, bau khas, rasa pahit sepat dengan tekstur kental. Simplisia kulit batang nangka memiliki tiga lapisan warna yaitu abu-abu kehijauan dengan bercak putih, lapisan orange dan lapisan coklat muda dengan permukaan tidak rata dan tebal kulit batang kurang lebih 1 cm, serbuk simplisia kulit batang nangka memiliki fragmen kristal oksalat bentuk prisma, serabut, jaringan gabus hablur, dan  parenkim dengan amilum. Ekstrak etil asetat mengandung senyawa flavonoid, dan alkaloid. Dan parameter non spesifik ekstrak etil asetat kulit batang nangka yaitu kadar air 16,97%, susut pengeringan 10,48%, kadar abu 9,78%, kadar abu tidak larut asam 1,58% dan bobot jenis 0,912. Dengan kadar flavonoid ekstrak etil asetat adalah 28,1025 µg/mL.
DETERMINATION OF SUN PROTECTION FACTOR (SPF) VALUE IN LIME (CITRUS AURANTIFOLIA) PEEL EXTRACT USING UV-VIS SPECTROPHOTOMETRY METHOD AM Andy Suryadi; Mahdalena SY Pakaya; Endah Nurrohwinta Djuwarno; Julianty Akuba
Jambura Journal of Health Sciences and Research Vol 3, No 2 (2021): JULI: JAMBURA JOURNAL OF HEALTH SCIENCES AND RESEARCH
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35971/jjhsr.v3i2.10319

Abstract

Lime (Citrus aurantifolia) is a plant that contains phenolic compounds, such as phenols, flavonoids, and tannins. With this content, lime peel extract has the potential as a sunscreen and has antioxidant activity. Sunscreen compounds refer to ones that can protect the skin from the effect of ultraviolet radiation. At first, the peel was extracted by maceration method using three solvents with different polarity (n-hexane, ethyl acetate, and 70% ethanol). The preliminary test found that the ethyl acetate and ethanol 70% extracts positively contained phenol, tannin, and flavonoid compounds, which were marked by a color change in the sample and continued to test the sunscreen activity. Further, the sunscreen activity test was determined based on the determination of the Sun Protection Factor (SPF) value that was done at 290-320 nm (wavelength) with a 5 nm interval.  The results showed that the sunscreen activity of the peel extract is very good at a concentration of 200 ppm for ethyl acetate extract with a value of 28.8; meanwhile, ethanol extracts 70% and ethyl acetate extracts of 400 and 600 ppm concentrations with an average SPF value of 24, 42.5, 42.2 and 81.8, respectively, are included in ultra protection because they have an SPF value of 15.     
ANTIBACTERIAL POTENTIAL TEST IN AGARWOOD (Gyrinops versteegii) STEM EXTRACT TOWARDS Escherichia coli and Staphylococcus aureus. Mahdalena Sy Pakaya; Moh. Adam Mustapa; Muthiah Rahmah Ali
Jambura Journal of Health Sciences and Research Vol 4, No 1 (2022): JANUARI: JAMBURA JOURNAL OF HEALTH SCIENCES AND RESEARCH
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35971/jjhsr.v4i1.11585

Abstract

Infection disease is one of the most common health problem in many developing countries, including Indonesia where the contamination of microbe is associated with the cause of the infection. The infection can be caused by various microorganisms such as bacteria, virus, fungus, and protozoa. Fortunately, Indonesia's medicinal plant varies and one of which is agarwood (Gyrinops versteegii). This study aims at measuring the potential of agarwood (Gyrinops versteegii) stem extract towards Escherichia coli and Staphylococcus aureus. In this study, the simplicial of agarwood stem is extracted by applying multilevel maceration method with n-Hexane solvent, ethyl acetate, and methanol. The result of antibacterial activity testing over three extracts reveals that the ethyl acetate extract can inhibit the growth of Escherichia coli dan Staphylococcus aureus. In addition, the result of screening in the ethyl acetate extract discovers that in contains alkaloid, terpenoid, and tannin, where the positive control used is chloramphenicol and the negative control is Dimethyl Sulfoxide (DMSO). The result of Minimum Inhibitory Concentration test in the agarwood stem extract towards Escherichia coli dan Staphylococcus aureus shows that the concentration of 50% is marked with absence of turbidity, whereas 50% in the Lowest Lethal Dose value signifies absence of growth in the bacteria in the Nutrient Agar (NA) media. Additionally, the result of potential test of agarwood stem ethyl acetate extract towards Escerichia coli in concentrations of 25%, 50%, and 100% with an inhibition zone created of 7,85 mm where it includes in medium category, and 10,6 mm and 13,45 mm that include in strong category. In the meantime, the result of potential test of agarwood stem ethyl acetate extract towards Staphylococcus aureus in concentrations of 25%, 50%, and 100% with an inhibition zone created of b,1 mm where it includes in medium category, and 13,2 mm and 16 mm that include in strong category in inhibiting bacteria.
UJI AKTIVITAS ENZIM DIASTASE MADU HUTAN MENTAH GORONTALO SEBAGAI IMUNOMODULATOR Juliyanty Akuba; Mahdalena Sy Pakaya
Pharmasipha: Pharmaceutical Journal of Islamic Pharmacy Vol 4, No 2 (2020): September
Publisher : University Of Darussalam Gontor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.307 KB) | DOI: 10.21111/pharmasipha.v4i2.4852

Abstract

Penggunaan Madu sebagai suatu bahan yang dapat menigkatkan daya tahan tubuh sudah banyak dipublikasikan, penjualan madu pada masyarakat luas pun sudah sangat banyak, bahkan ada beberapa orang yang sudah membuat sebuah brand untuk produk madu itu sendiri. Akan tetapi suatu madu bisa dikatakan sebagai imunomodulator harus memenuhi persyaratan, diantaranya nilai dari Aktivitas Enzim diastase minimal 3 DN. Tujuan dari penelitian ini adalah untuk melihat apakah madu hutan Gorontalo bisa digunakan sebagai imunomodulator. Penelitian ini menggunakan 3 madu dari 3 lokasi tempat yang berbeda, yaitu dari daerah Paguat, Lemito dan Bone Pantai, semua lokasi berada di Provinsi Gorontalo. Pada penelitian ini  dilakukan pemeriksaan organoleptis, setelah itu dilakukan pengujian aktivitas enzim diastase menggunakan spektrofotometer. Hasil penelitian menunjukkan rata – rata nilai dari aktivitas enzim diastase dari ke 3 sampel yang diperiksa menunjukkan hasil sebesar 2,8571 DN. Kesimpulan bahwa sampel madu dari ke 3 daerah tersebut belum bisa dikategorikan sebagai imunomodultor.
POTENSI EKSTRAK ETANOL KULIT BUAH MATOA (Pometia pinnata J.R Forst & G.Forst) TERHADAP BAKTERI PENYEBAB KARIES GIGI Mahdalena Sy Pakaya; Jihan Astuti Kai; Wiwit Zuriati Uno
Jambura Journal of Chemistry Vol 3, No 2 (2021): August
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34312/jambchem.v3i2.11204

Abstract

One of the infectious diseases that occur in the oral cavity is dental caries (cavities). The bacteria that play a role in the occurrence of infection are Streptococcus mutans bacteria, and the matoa plant is one of the plants that can act as antibacterial (Pometia pinnata J.R Forst) which has high efficacious compounds. This study aimed to determine the activity and potency of the ethanolic extract of matoa fruit peel (Pometia pinnata J.R Forst & G. Forst) against Streptococcus mutans bacteria. This research is a laboratory experimental research. Matoa fruit peel simplicia was extracted by maceration method with 96% ethanol solvent, then tested for phytochemical screening to determine the class of compounds contained in the matoa fruit peel. Furthermore, the activity was tested against Streptococcus mutans bacteria using a positive control of chloramphenicol antibiotics and a negative control of Dimethyl Sulfoxide. After that, the values of the Minimum Inhibitory Level and Minimum Killer were measured. From these results, it was continued to test the antibacterial potential using the Kirby Bauer method. The results showed that the ethanol extract of matoa fruit peel (Pometia pinnata J.R Forst & G. Forst) had activity against Streptococcus mutans bacteria. The potential value of the matoa fruit peel extract against Streptococcus mutans bacteria at concentrations of 25%, 50% and 75%, respectively, was 11.75 mm, 15.82 mm and 18.75 mm which was included in the category of strong inhibition.
Co-Authors A. Mu’thi Andy Suryadi Abas, Siti Nur Rahmatiya Abdullah, Rahayu Anatasya Putri Ade Ari Puspitadewi Akuba, Juliyanti Ali, Muthiah Rahmah Aliya Ibrahim Ibrahim alkatiri, ahmad alkatiri AM Andy Suryadi AM. Andy Suryadi Andi Makkulawu Ariani H. Hutuba Arista Idris, Pinkan Arona Salama Dewa Ayu Puspita Dewi Rahmawaty Moo Dizky Ramadani Papeo djuarno, endah nurrohwinta Djuwarno, Endah Nurrowinta Dr. Apt. Hamsidar Hasan S.Si.,M.Si dwi pratiwi, susi Endah Nurrohwinta Djuwarno Fajar Dwi Agung Faradila Ratu Cindana Fatimah Azzahra Fika Nuzul Ramadhani Grasela Mbae Hamsidar Hasan Hamsidar Hasan Hamsidar Hasan Handriansyah pakaya Hartati Hartati Hendrawan Dwikarunia Datukramat Hiola, Faramita Hunawa, Rachmawaty Dirham I Made Hariadi Wijaya Ibrahim, Annisa Humairah Jihan Astuti Kai Julianty Akuba Khofifa Nurul Magfira Maspeke La Ode Aman Latif, Multiani S. Liberto, Frith F. Lisa Efriani Puluhulawa Madania Madania, Madania Madania, Madania Magfirah Nur Cahyani Makkulawu, Andi Manno, Mohamad Reski Moh. Adam Mustapa Mohamad Adam Mustapa Mohamad Aprianto Paneo Mohamad Reski Manno Muhammad Taupik Muhammad Taupik Multiani S. Latif Mustapa, Moh. Adam Muthiah Rahmah Ali Nazwah Buana Putri Kalangi Niluh Sri Purnama Nur Ain Thomas Nur Rasdianah Nurdiandra Jahja Pakaya, Meri Isriani Papeo, Dizky Ramadani Putri Papeo, Pirdawati priliya wati d.as'ali Puluhulawa, Lisa Efriani Rahmawati Hunawa Rahmulia Thamrin Ramly Abudi Robert Tungadi Santi Santi Selvi Marcellia Suryadi,, A. Mu'thi Andy Teti Sutriyati Tuloli Teti Sutriyati Tuloli Tolulu, Sri Noviani umar, linsih Wicita, Prisca S. Widy Susanti Abdulkadir Widy Susanti Abdulkadir Widysusanti Abdulkadir Wiwit Zuriati Uno Yayah Rachmatiyah Yunus, Fitriah Ayu Magfirah