Article Per Year (5 Year)
p-Index From 2020 - 2025
0.408
P-Index
This Author published in this journals
All Journal Jurnal Bioteknologi & Biosains Indonesia
Ariza Yandwiputra Besari, Ariza Yandwiputra
Research Center for Biotechnology, Indonesian Institute of Sciences
Biochemistry, Genetics & Molecular Biology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
EVALUATION AND INHIBITORY MECHANISM ANALYSIS OF NATURAL COMPOUNDS AGAINTS DIHYDROOROTATE DEHYDROGENASE AS ANTI-CANCER AGENTS Wibowo, Aji; Waluyo, Danang; Tejo, Bimo Ario; Komariyah, Tinta; Besari, Ariza Yandwiputra; Prabandari, Erwahyuni Endang
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2842

Abstract

Cancer remains one of the deadliest diseases worldwide, and currently cancer treatment is facing several problems related to adverse effects and drug resistance. To address these problems, new prospective anticancer medications are required. Natural compounds, which have been extensively used in the drug research, including for the treatment of cancer, are emerging as viable candidates. This study aimed to evaluate 33 in-house natural compounds against dihydroorotate dehydrogenase (DHODH) enzyme, a viable target to develop anticancer agent, and to analyze the hit inhibitory mechanism against protein target. In the activity assay, atovaquone was the sole substance to have activity against DHODH, with an inhibition rate of 47.44% at 10 µM. However, discrepancies were shown in the molecular docking result, where atovaquone were identified as hits. Molecular dynamic analysis revealed that atovaquone initially bound to the active site before being forced to the outside due to cleavage of hydrogen bond between the ligand and responsible residue. This study clearly demonstrated the importance of molecular dynamic analysis to study inhibitory mechanism of compound against target protein that may be useful for further development.
INHIBITORY ACTIVITY OF INDONESIAN MICROBIAL EXTRACTS AGAINST PROLIFERATION OF DLD-1 COLORECTAL CANCER CELL LINE Besari, Ariza Yandwiputra; Waluyo, Danang; Komariyah, Tinta; Prabandari, Erwahyuni Endang
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2851

Abstract

Colorectal cancer (CRC) is the second deadliest cancer in the world. Several anti-cancer agents are currently used for the clinical treatment of CRC. However, toxicity and drug resistance pose significant challenges in CRC chemotherapy. On the other hand, microbe-derived natural products have been explored as a source for the development of anti-cancer therapeutic agents. This study aimed to examine the potential of the microbial library in BioMCC (Biotech Center-BPPT Microbial Culture Collection) as a source for anti-cancer drug discovery. Among the 720 fungal extracts tested, 60 extracts (8.3%) showed inhibitory activity against the proliferation of the colorectal carcinoma DLD-1 cell line, while not affecting Vero cells (African green monkey kidney normal cell line). One of these active extracts was derived from the fungus Sporothrix sp. BioMCC-f.T.7716. Although the inhibitory mechanism of this extract against the proliferation of the DLD-1 cell line could not be determined, this study clearly demonstrated the potential use of Indonesian microbial extracts as a source for the discovery of anti-cancer agents.
Co-Authors Aji Wibowo ERWAHYUNI ENDANG PRABANDARI Komariyah, Tinta Tejo, Bimo Ario Waluyo, Danang