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TEMPERATURE EFFECTS ON THE STABILITY OF MYCOBACTERIUM TUBERCULO-SIS SHIKIMATE KINASE (MtSK): STRATEGIES FOR SECURE TRANSPORT Wibowo, Aji; Komariyah, Tinta; Prabandari, Erwahyuni Endang; Ariyani, Titin; Siska, Eka
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.5751

Mycobacterium tuberculosis Shikimate Kinase (MtSK) has a crucial role in the shikimic pathway, which is essential for this bacteria but is absent in humans, making it a potential target for novel anti-tuberculosis drugs. This study used enzyme-coupled fluorescence to examine the stability of MtSK stored in 50% glycerol at -30 ℃, 4 ℃, and ±28 ℃ for six days. Results showed stable enzyme activity values (α=0.05) at all temperatures. This research underscores that MtSK’s stability depends on its molecular properties, including GC content, hydrophobic residues, Mg2+ binding, and intra-helical salt bridge. Despite some activity decline over time due to glycerol-induced aggregation, MtSK can be safely transported at ±28 ℃ for up to six days without special cooling compartment. Understanding MtSK stability ensures its active conformation remains consistent, reducing off-target effects on drug design and enhancing drug efficacy. This insight ultimately leads to high-quality and commercially viable tuberculosis treatment development. Future research should explore MtSK stability at higher temperatures and assess the optimal glycerol content for cryopreservation.

EVALUATION AND INHIBITORY MECHANISM ANALYSIS OF NATURAL COMPOUNDS AGAINTS DIHYDROOROTATE DEHYDROGENASE AS ANTI-CANCER AGENTS Wibowo, Aji; Waluyo, Danang; Tejo, Bimo Ario; Komariyah, Tinta; Besari, Ariza Yandwiputra; Prabandari, Erwahyuni Endang
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2842

Cancer remains one of the deadliest diseases worldwide, and currently cancer treatment is facing several problems related to adverse effects and drug resistance. To address these problems, new prospective anticancer medications are required. Natural compounds, which have been extensively used in the drug research, including for the treatment of cancer, are emerging as viable candidates. This study aimed to evaluate 33 in-house natural compounds against dihydroorotate dehydrogenase (DHODH) enzyme, a viable target to develop anticancer agent, and to analyze the hit inhibitory mechanism against protein target. In the activity assay, atovaquone was the sole substance to have activity against DHODH, with an inhibition rate of 47.44% at 10 µM. However, discrepancies were shown in the molecular docking result, where atovaquone were identified as hits. Molecular dynamic analysis revealed that atovaquone initially bound to the active site before being forced to the outside due to cleavage of hydrogen bond between the ligand and responsible residue. This study clearly demonstrated the importance of molecular dynamic analysis to study inhibitory mechanism of compound against target protein that may be useful for further development.

INHIBITORY ACTIVITY OF INDONESIAN MICROBIAL EXTRACTS AGAINST PROLIFERATION OF DLD-1 COLORECTAL CANCER CELL LINE Besari, Ariza Yandwiputra; Waluyo, Danang; Komariyah, Tinta; Prabandari, Erwahyuni Endang
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2851

Colorectal cancer (CRC) is the second deadliest cancer in the world. Several anti-cancer agents are currently used for the clinical treatment of CRC. However, toxicity and drug resistance pose significant challenges in CRC chemotherapy. On the other hand, microbe-derived natural products have been explored as a source for the development of anti-cancer therapeutic agents. This study aimed to examine the potential of the microbial library in BioMCC (Biotech Center-BPPT Microbial Culture Collection) as a source for anti-cancer drug discovery. Among the 720 fungal extracts tested, 60 extracts (8.3%) showed inhibitory activity against the proliferation of the colorectal carcinoma DLD-1 cell line, while not affecting Vero cells (African green monkey kidney normal cell line). One of these active extracts was derived from the fungus Sporothrix sp. BioMCC-f.T.7716. Although the inhibitory mechanism of this extract against the proliferation of the DLD-1 cell line could not be determined, this study clearly demonstrated the potential use of Indonesian microbial extracts as a source for the discovery of anti-cancer agents.

TEMPERATURE EFFECTS ON THE STABILITY OF MYCOBACTERIUM TUBERCULO-SIS SHIKIMATE KINASE (MtSK): STRATEGIES FOR SECURE TRANSPORT Wibowo, Aji; Komariyah, Tinta; Prabandari, Erwahyuni Endang; Ariyani, Titin; Siska, Eka
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.5751

Mycobacterium tuberculosis Shikimate Kinase (MtSK) has a crucial role in the shikimic pathway, which is essential for this bacteria but is absent in humans, making it a potential target for novel anti-tuberculosis drugs. This study used enzyme-coupled fluorescence to examine the stability of MtSK stored in 50% glycerol at -30 ℃, 4 ℃, and ±28 ℃ for six days. Results showed stable enzyme activity values (α=0.05) at all temperatures. This research underscores that MtSK’s stability depends on its molecular properties, including GC content, hydrophobic residues, Mg2+ binding, and intra-helical salt bridge. Despite some activity decline over time due to glycerol-induced aggregation, MtSK can be safely transported at ±28 ℃ for up to six days without special cooling compartment. Understanding MtSK stability ensures its active conformation remains consistent, reducing off-target effects on drug design and enhancing drug efficacy. This insight ultimately leads to high-quality and commercially viable tuberculosis treatment development. Future research should explore MtSK stability at higher temperatures and assess the optimal glycerol content for cryopreservation.

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