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All Journal Indonesian Journal of Science and Technology Jurnal Biologi Tropis Jurnal Biogenerasi
Hari Hariadi
Badan Riset dan Inovasi Nasional
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Other

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KARAKTERISTIK BIOKIMIA DAN MIKROBIOLOGI PADA LARUTAN FERMENTASI KEDUA KOMBUCHA BUNGA TELANG (Clitoria ternatea L) SEBAGAI INOVASI PRODUK BIOTEKNOLOGI TERKINI M. Fariz Fadillah; Hari Hariadi; K kusumiyati; Firman Rezaldi; Diyan Yunanto Setyaji
Jurnal Biogenerasi Vol. 7 No. 2 (2022): Jurnal Biogenerasi Vol 7 Nomor 2 tahun 2022
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v7i2.1765

Abstract

Salah satu kandungan bunga telang (Clitoria ternatea L) yaitu berupa antosianin yang memiliki potensi sebagai antioksidan dan antibakteri. Bunga telang dapat diolah menjadi minuman kombucha yang merupakan inovasi produk bioteknologi terkini dari kayak nya karakteristik biokimia dan mikrobiologi. Penambahan madu jenis Clover Honey dapat dimanfaatkan sebagai pemanis serta sumber nutrisi yang baik bagi tubuh dalam pembuatan minuman kombucha bunga telang pada fermentasi kedua. Oleh karena itu, dalam penelitian ini kombucha bunga telang yang telah dipanen selama 2 minggu lalu ditambahkan madu Clover Honey selama waktu tambahan 7 hari yaitu 21 hari. Metode yang digunakan dalam penellitian ini adalah Rancangan Acak Kelompok (RAK) yang dibagi menjadi dua faktor. Faktor I merupakan fermentasi kombucha bunga telang berumur 1 minggu pada konsentrasi gula (20%, 30%, dan 40%) dan faktor II yaitu fermentasi kombucha bunga telang dengan penambahan madu Clover Honey pada konsentrasi madu (20%, 30%, dan 40%). Data yang diperoleh dari penelitian dianalisis menggunakana ANOVA dan apabila terdapat interaksi antara kedua faktor maka dilanjutkan dengan uji lanjut BNT (Beda Nyata Terkecil) atau DMRT 5%. Hasil yang telah diperoleh setelah kombucha bunga telang difermentasi selama 21 hari yaitu konsentrasi madu berpengaruh nyata (α= 0,05) terhadap nilai total asam, pH, total fenol, total total gula, dan total mikroba. Perlakuan terbaik diperoleh yaitu pada adalah konsentrasi 40% yaitu total asam 1,88%, total fenol 115,71 ppm, total gula 23.53%, dan total mikroba 8,01 log CFU/mL. Perlakuan terbaik untuk mengetahui karakteristik mikrobiologi pada kombucha bunga telang (Clitoria ternatea L) selama fermentasi kedua dan pasca ditambahkan madu Clover Honey adalah konsentrasi 40%. Aktivitas antibakteri pada Staphylococcus aureus memiliki rata-rata diameter zona hambat sebesar 17,54 mm dengan kategori kuat. Pada bakteri Staphylococcus epidermidis zona hambat sebesar 16,73 mm dengan kategori kuat. Zona hambat sebesar 15,96 mm pada bakteri Pseudomonas aeruginosa dengan kategori kuat. 15,26 mm pada bakteri Escherichia coli dengan kategori kuat. Karakteristik biokimia dan mikrobiologi yang dihasilkan oleh kombucha bunga telang melalui fermentasi yang kedua merupakan salah satu terobosan baru untuk menciptakan inovasi produk bioteknologi terkini.
Biochar from Agricultural Waste for Soil Amendment Candidate under Different Pyrolysis Temperatures Abdul Mutolib; Ali Rahmat; Endra Triwisesa; H. Hidayat; Hari Hariadi; Kiki Kurniawan; S. Sutiharni; S. Sukamto
Indonesian Journal of Science and Technology Vol 8, No 2 (2023): (ONLINE FIRST) IJOST: September 2023
Publisher : Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17509/ijost.v8i2.55193

Abstract

Indonesia as an agricultural country produces and consumes a huge amount of fruits. One of the popular fruits is durian (Durio zibethinus), which can create issues with the high amount of durian seeds. Durian seeds can be fermented quickly. Thus, if they are not processed immediately, it causes pollution to the environment, particularly an odor issue. One of the waste management is to convert durian seed waste into biochar, an ameliorant agent to improve soil quality. This study aims to analyze the physicochemical properties of durian seed-derived biochar under different pyrolysis temperatures. The results showed that the increases in the temperature allowed the creation of more porosity in the biochars, which is due to chemical structure and crystallinity arrangement. This is confirmed by the increases in surface area and total pore volume also decreases in particle size. The change in the chemical structure can be verified by the decreases in the biochar yield. The produced biochar from fruit seeds has excellent carbon content and elemental components such as potassium, magnesium, phosphor, and sulfur, informing prospective fruit seeds as a soil amendment fertilizer
Effect of Biotechnological Fermentation Waste Kombucha Flower Telang (Clitoria ternatea L) as Liquid Fertilizer on The Growth of Sawey (Brassica chinensis var. parachinensis) Hari Hariadi; Firman Rezaldi; Fajar Hidayanto; Ade Sumiardi; Mu'jijah Mu'jijah; Muhammad Faizal Fathurrohim; Yuliana Kolo; Syariful Mubarok
Jurnal Biologi Tropis Vol. 23 No. 3 (2023): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i3.5081

Abstract

Liquid organic fertilizer comes from nature and has the potential to improve biological, physical and soil properties because it contains nutrients that have the potential to increase plant growth. This research was conducted to determine liquid fertilizer made from telang kombucha fermentation waste on the growth of mustard plants. The method used was a completely randomized design with a single factor, namely the difference in the concentration of liquid organic fertilizer given 6 treatments with 5 replications for each treatment. The research data were analyzed statistically through ANOVA at a significance level of 95%. Parameters in this study were plant height, number of leaves, fresh weight and dry weight. The results of this study prove that the spraying treatment of liquid organic fertilizer containing kombucha fermentation waste of butterfly pea flowers has an effect on the growth parameters of mustard plants. The conclusion is that liquid organic fertilizer from the waste of fermented kombucha flower butterfly pea at a concentration of 5 mL/L can increase plant height, number of leaves, wet and dry weight of mustard plants.
Growth Inhibition Test of Gram and Negative Bacteria in Pharmaceutical Biotechnology Products in the Form of Hand Sanitizer Formulations Based Fermented Telang Flower Kombucha Hari Hariadi; Muhammad Andry; Muhammad Amin Nasution; Ade Sumiardi; Firman Rezaldi; Suseno Amien; Yusep Ikrawan
Jurnal Biologi Tropis Vol. 23 No. 3 (2023): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i3.5219

Abstract

Telang flower kombucha is proven to have various pharmacological activities, so that it can be developed as medicines, cosmetics, and its waste can be applied in agriculture as an ingredient in organic liquid fertilizer to support plant development and growth. This research aims to make hand sanitizer gel formulations and preparations with active butterfly pea flowers. Disk diffusion is used to stop gram-positive and gram-negative bacteria from growing. Post hoc analysis and one-way ANOVA were used to analyze the data. The one-way ANOVA analysis yielded results with a significance level of P<0.05, allowing the post hoc test to continue. When tested at a sugar concentration of 40%, the butterfly pea flower kombucha hand sanitizer significantly outperformed the control when it came to inhibiting the growth of the test bacteria. The best way to stop the test bacteria from growing is to use a concentration of 40%.
Co-Authors Abdul Mutolib Ade Sumiardi Ali Rahmat Diyan Yunanto Setyaji Endra Triwisesa Firman Rezaldi Firman Rezaldi H. Hidayat Kiki Kurniawan Kusumiyati M. Fariz Fadillah Mochammad Imron Awalludin Mu’jijah Muhammad Amin Nasution Muhammad Andry Muhammad Faizal Fathurrohim S. Sukamto Suseno Amien Sutiharni Syariful Mubarok Yuliana Kolo Yusep Ikrawan