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All Journal VALENSI Jurnal AgroBiogen Current Biochemistry Indonesian Journal of Chemistry 3BIO: Journal of Biological Science, Technology and Management
Inda Setyawati, Inda
Department Of Biochemistry, Faculty Of Mathematics And Natural Sciences, IPB University, Bogor, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Immunology & microbiology Neuroscience

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Characteristics of Glucose Oxidase Gene (GGOx) from Aspergillus niger IPBCC 08.610 Popi Asri Kurniatin; Laksmi Ambarsari; Annisa Dhiya Athiyyah Khanza; Inda Setyawati; Djarot Sasongko Hami Seno; Waras Nurcholis
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 6, No. 1, May 2020
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (763.755 KB) | DOI: 10.15408/jkv.v6i1.9440

Abstract

Glucose oxidase is used in various industries for the development of enzymatic fuel cell. Based on prior studies, this compound is sourced from the local isolates of Aspergillus niger IPBCC 08.610, although investigations on the encoding gene have not been conducted. The purpose of this research, therefore, is to identify and characterized the gene responsible for encoding glucose oxidase, in the aspect of sequence, length, and restriction patterns. This experiment involved the amplification of genomic DNA using specific primers for gene recognition, which was followed by the restriction technique with EcoRI and PstI endonucleases. Furthermore, the gene is inserted into vector pGEM®T-Easy and transformed into competent E. coli DH5α cells, in an attempt to perform sequencing. The glucose oxidase gene from A. niger IPBCC 08.610 was confirmed to possess a size of 1848 bp, and a GC content of 57.8%, with a possibility of restriction into two fragments of size 908 bp and 980 bp, using the EcoRI restriction.
Bioethanol Production by Using Detoxified Sugarcane Bagasse Hydrolysate and Adapted Culture of Candida tropicalis Inda Setyawati; Laksmi Ambarsari; Siti Nur'aeni; Suryani Suryani; Puspa Julistia Puspita; Popi Asri Kurniatin; Waras Nurcholis
Current Biochemistry Vol. 2 No. 1 (2015)
Publisher : IPB University

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Abstract

Ethanol is considered as the most promising alternative fuel, since it can be produced from a variety of agriculturally-based renewable materials, such as sugarcane bagasse. Lignocellulose as a major component of sugarcane bagasse is considered as an attractive renewable resource for ethanol production due to its great availability and relatively low cost. The major problem of lignocellulose is caused by its need for treatment to be hydrolyzed to simple sugar before being used for bioethanol production. However, pretreatment using acid as hydrolyzing agent creates some inhibitor compounds that reduce ethanol production because these compounds are potential fermentation inhibitors and affect the growth rate of the yeast. Reduction of these by-products requires a conditioning (detoxification and culture starter adaptation). Thus, the aim of this study was to evaluate bioethanol production by fermentation with and without detoxified sugarcane bagasse acid hydrolysate using adapted and non-adapted culture of C. tropicalis. According to this study, the highest ethanol amount was obtained about 0.43 % (v/v) with an ethanol yield of 2.51 % and theoretical yield of 4.92 % by fermentation of sugarcane bagasse hydrolysate with detoxification using the adapted strain of C. tropicalis at 72 hours fermentation time. Furthermore, the addition of 3 % glucose as co-substrate on detoxified-hydrolysate media only achieved the highest ethanol concentration 0.21 % after 24 hours fermentation with the ethanol yield 0.69 % and theoretical ethanol yield 1.35 %, thus it can be concluded that the addition of glucose could not increase the ethanol production.
Keragaman Genetik Rizobakteri Penghasil Asam Indol Asetat Berdasarkan 16S rRNA dan Amplified Ribosomal DNA Restriction Analysis Puji Lestari; Dwi N. Susilowati; I Made Samudra; Tri P. Priyatno; Kristianto Nugroho; Whyranti Nurarfa; Inda Setyawati; Yadi Suryadi
Jurnal AgroBiogen Vol 13, No 1 (2017): Juni
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v13n1.2017.p25-34

Abstract

Asam indol asetat (AIA) dapat dihasilkan oleh bakteri rizosfer/rizobakteri pemacu pertumbuhan tanaman (PPT). Keragaman genetik isolat bakteri PPT indigenous Indonesia perlu diinvestigasi untuk mencari sumber potensial agen PPT dengan informasi kekerabatan intra dan interspesies yang jelas. Karena itu penelitian ini bertujuan mengetahui keragaman genetik rizobakteri penghasil AIA indigenous Indonesia dengan gen 16S rRNA, dilengkapi dengan ARDRA. Koleksi isolat bakteri BB Biogen diidentifikasi kandungan AIA-nya, morfologi secara makroskopis dan sekuensing pada sekuen 16S rRNA dan ARDRA. Total empat belas isolat rizobakteri memiliki kandungan AIA dalam kisaran 5,24-37,69 µg/ml dan tertinggi pada SM1. Karakteristik morfologi koloni rizobakteri mendukung variasi strain bakteri penghasil AIA. Delapan isolat terpilih diidentifikasi sebagai spesies Bacillus dengan homologi 96-99%. Lima isolat (SM1, JP4, KP3, MB2, dan CP3) diidentifikasikan sebagai B. subtilis, SC2 sebagai B. amyloliquefaciens, BL2 dekat dengan B. velezensis, dan JP3 memiliki homologi tinggi dengan Brevundimonas olei. Delapan isolat rizobakteri tersebut berkerabat dekat dengan strain bakteri referensi yang memiliki kesamaan spesies. Analisis ARDRA-RsaI menghasilkan lima filotipe dengan keunikan pola sidik jari. Isolat CP3, MB 2, dan KP 3 berada dalam satu filotipe. Kedekatan isolat dalam Bacillus sp. digambarkan oleh filotipe 5 (B. subtilis SM1 dan B. velezensis BL2) yang diduga jauh dari B. amyloliquefaciens SC2 (filotipe 4) dan JP 3 pada genus Brevundimonas (filotipe 3). Keragaman genetik isolat rizobakteri penghasil AIA terhitung rendah berdasarkan 16S-rRNA dan ARDRA-RsaI.
Immobilization of Glucose Oxidase on Modified-Carbon-Paste-Electrodes for Microfuel Cell Laksmi Ambarsari; Inda Setyawati; Rini Kurniasih; Popi Asri Kurniatin; Akhiruddin Maddu
Indonesian Journal of Chemistry Vol 16, No 1 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (368.241 KB) | DOI: 10.22146/ijc.21183

Abstract

Glucose oxidase (GOx) is being developed for many applications such as an implantable fuel cell, due to its attractive property of operating under physiological conditions. This study reports the functional immobilization of glucose oxidase onto polyaniline-nanofiber-modified-carbon-paste-electrodes (GOx/MCPE) as bioanodes in fuel cell applications. In particular, GOx is immobilized onto the electrode surface via a linker molecule (glutaraldehyde). Polyaniline, synthesized by the interfacial polymerization method, produces a morphological form of nanofibers (100-120 nm) which have good conductivity. The performance of the polyaniline-modified-carbon-paste-electrode (MCPE) was better than the carbon- paste-electrode (CPE) alone. The optimal pH and temperature of the GOx/MCPE were 4.5 (in 100 mM acetate buffer) and 65 °C, respectively. The GOx/MCPE exhibit high catalytic performances (activation energy 16.4 kJ mol-1), have a high affinity for glucose (Km value 37.79 µM) and can have a maximum current (Imax) of 3.95 mA. The sensitivity of the bioelectrode also was high at 57.79 mA mM-1 cm-2.
Region-Specific Thermal Stability of Kelulut Honey from Indonesia: Implications for Quality and Processing Setyawati, Inda; Meirila, Salvinia Fara; Febriyanto, Dimas; Andriyanto, Dimas
3BIO: Journal of Biological Science, Technology and Management Vol. 7 No. 2 (2025)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2025.7.2.2

Abstract

Kelulut honey, produced by stingless bees (Trigona spp.), is valued for its nutritional and medicinal benefits. However, thermal treatment can alter its physicochemical properties, affecting quality and functionality. This study assessed the impact of heating at 65 °C and 121 °C for 5 minutes on Kelulut honey from seven regions in Indonesia, analyzing changes in color intensity, total dissolved solids (TDS), solubility, viscosity, water content, hydroxymethylfurfural (HMF), and acidity. At 65°C, solubility improved (e.g., Garut: 22.7 → 84.7) and TDS increased (Bogor: 77.0 % → 79.5 %), while 121 °C treatment led to significant HMF accumulation (mean: 25.91 → 40.16 mg/kg) and viscosity fluctuation. Klaten honey uniquely darkened at 121 °C, indicating regional compositional differences. Acidity dropped by up to 21% in Sumedang and Garut, while Bangka maintained stability. This study demonstrates that thermal treatment significantly influences the physicochemical quality of Kelulut honey and underscores the need for region-specific processing guidelines to maintain product integrity for food and medicinal applications, and that HMF, viscosity, and TDS could serve as reliable markers for assessing thermal effects in honey.
Co-Authors Akhiruddin Maddu Andriyanto, Dimas Annisa Dhiya Athiyyah Khanza Djarot Sasongko Hami Seno Dwi N. Susilowati Febriyanto, Dimas I Made Samudra Kristianto Nugroho LAKSMI AMBARSARI Meirila, Salvinia Fara Puji Lestari Puspa Julistia Puspita Rini Kurniasih, Rini Siti Nur'aeni Suryani Suryani Tri P. Priyatno Waras Nurcholis Whyranti Nurarfa Yadi Suryadi