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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) VALENSI Jurnal Penelitian Pascapanen Pertanian Current Biochemistry
Puspa Julistia Puspita
Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education

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In Silico Analysis of Glucose Oxidase H516r and H516d Mutations for an Enzymatic Fuel Cell Puspa Julistia Puspita; Laksmi Ambarsari; Adrian Adiva; Tony Ibnu Sumaryada
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 7, No. 2, November 2021
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v7i2.20733

Abstract

Glucose oxidase (GOx) is an oxido-reductase enzyme that catalyzes glucose into hydrogen peroxide and glucono delta-lactone (GDL). GOx has the potential to be used in the medical field. Numerous research concerning the usage of GOx to create enzymatic biofuel cells have been done, nevertheless the results obtained have not been optimal. This research aims to increase the Km values of GOx in order to increase its potential as a material for an enzymatic fuel cell. The amino acid histidine in position 516 is a residue in the active site that plays an important part in the process of glucose oxidation. In this research we mutated H516 by in silico twice resulting in the mutants R516 and D516. The mutations resulted in a change of the docking area for both mutants and in the docking affinity for H516D resulting in higher Km values. This shows that the H516 residue plays an important part in the functions of glucose oxidase and mutation into aspartate could improve glucose oxidase based enzymatic fuel cells.
EXTRACTION, CHARACTERIZATION AND APPLICATION OF CHITOSAN DERIVED FROM MAGGOT (BLACK SOLDIER FLY) WASTE AS EDIBLE COATING FOR RED GRAPES (Vitis vinifera) Sri Wahyuni; Ranti Selvina; Puspa Julistia Puspita; Haryo Tejo Prakoso; Priyono priyono; Siswanto siswanto
Jurnal Penelitian Pascapanen Pertanian Vol 18, No 1 (2021): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v18n1.2021.45-56

Abstract

Black Soldier Fly (BSF) farming showed an increasing trend in the past 5 years. The production process generates a leftover, that is the exuviae which are still untapped. BSF exuviae contain chitin and chitosan which can be utilized in various fields, one of which is fruit preservatives. Grapes (Vitis vinifera) is known to have many benefits and has a content of vitamins A, B, C, and E but it has a short shelf life. The purpose of this study was to characterize chitosan from BSF exuviae and choose the best edible coating method to be applied to grapes to prolong the grape’s storage time. Characterization includes physical condition, percent solubility, degree of deacetylation, and yield of the chitosan. Then, edible coating tests were carried out with 2% acetic acid solvent followed by two different methods, namely dyeing method with 5, 10 and, 15 minutes immersion time and spray method with a chitosan solution concentration of 0,5%, 1%, 1,5%, 2 %, and 2,5%. The results showed that chitosan extracted from BSF exuviae produced a physical appearance in the form of white brown powder with 55% solubility and a degree of deacetylation of 91,88%. The chitin yield is 16,2%, followed by the chitosan yield of 4,8%. The best edible coating method which can maintain the physical appearance of grapes and inhibiting fruit decayed was obtained by dipping method at a concentration of 2,5% for 10 minutes. Based on the overall observations, however, the results obtained from the dip and spray method were not significantly different.
Bioethanol Production by Using Detoxified Sugarcane Bagasse Hydrolysate and Adapted Culture of Candida tropicalis Inda Setyawati; Laksmi Ambarsari; Siti Nur'aeni; Suryani Suryani; Puspa Julistia Puspita; Popi Asri Kurniatin; Waras Nurcholis
Current Biochemistry Vol. 2 No. 1 (2015)
Publisher : IPB University

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Abstract

Ethanol is considered as the most promising alternative fuel, since it can be produced from a variety of agriculturally-based renewable materials, such as sugarcane bagasse. Lignocellulose as a major component of sugarcane bagasse is considered as an attractive renewable resource for ethanol production due to its great availability and relatively low cost. The major problem of lignocellulose is caused by its need for treatment to be hydrolyzed to simple sugar before being used for bioethanol production. However, pretreatment using acid as hydrolyzing agent creates some inhibitor compounds that reduce ethanol production because these compounds are potential fermentation inhibitors and affect the growth rate of the yeast. Reduction of these by-products requires a conditioning (detoxification and culture starter adaptation). Thus, the aim of this study was to evaluate bioethanol production by fermentation with and without detoxified sugarcane bagasse acid hydrolysate using adapted and non-adapted culture of C. tropicalis. According to this study, the highest ethanol amount was obtained about 0.43 % (v/v) with an ethanol yield of 2.51 % and theoretical yield of 4.92 % by fermentation of sugarcane bagasse hydrolysate with detoxification using the adapted strain of C. tropicalis at 72 hours fermentation time. Furthermore, the addition of 3 % glucose as co-substrate on detoxified-hydrolysate media only achieved the highest ethanol concentration 0.21 % after 24 hours fermentation with the ethanol yield 0.69 % and theoretical ethanol yield 1.35 %, thus it can be concluded that the addition of glucose could not increase the ethanol production.
The Addition Effects of Glucose as a Co-substrate on Xylitol Production by Candida guilliermondii Laksmi Ambarsari; Suryani Suryani; Steffanus Gozales; Puspa Julistia Puspita
Current Biochemistry Vol. 2 No. 1 (2015)
Publisher : IPB University

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Abstract

High cost production is one of the constraints of the commercial xylitol production due to high energy needed and pure raw materials. Therefore, it is necessary to improve the xylitol production eficiently with lower production cost by using microorganisms. The research objectives were to determine the optimum xylitol production from xylose by metabolism of C. guilliermondii and effect of glucose as a co-substrate in fermentation medium. The ratio of glucose : xylose (g/L) was 1:25, 1:12, 1:5 and 1:2.5 respectively. The xylitol concentration was measured by spectrophotometer method (D-sorbytol/D-xylitol kit). The result showed that the exponential phase of Candida guilliermondii was 12 h to 36 of incubation and optimum of incubation time to produce the highest xylitol was 72 h. The best ratio- of glucose : xylose to produce xylitol was 9 g/L glucose : 45 g/L xylose (1 : 5). The xylitol concentration produced from medium with the addition of glucose was 2.85 g/L. This concentration increased five times compared to that in the medium without addition of glucose that only reached 2.85 g/L. According to this study, the addition of glucose as a co-substrate could increase the xylitol production.
Antibacterial Activities of Sirih Merah (Piper crocatum) Leaf Extracts Puspa Julistia Puspita; Mega Safithri; Nirmala Peni Sugiharti
Current Biochemistry Vol. 5 No. 3 (2018)
Publisher : IPB University

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Abstract

Piper crocatum is one of medicinal herbal plants with a large number of benefits. Usually herbal plants have activity as antibacterial agent. Therefore, the objectives of this research were to obtain information on antibacterial activities of the leaf extracts of Piper crocatum againts four types of bacteria, in that Staphylococcus, Bacillus substilis, Escherichia coli, and Pseudomonas aeruginosa and then to analyze the phytochemistry of the leaf extracts of Piper crocatum. The leaves of Piper crocatum were extracted by maceration and reflux using ethanol 30%. The assays of the antibacterial activities and phytochemistry on the extracts were carried out using the method of Maria Bintang. Results showed that the yield of the extraction using ethanol by maceration method was 20.8%. Meanwhile, using the reflux method, the yield was obtained about 26.25%. The phytochemistry analysis showed that the leaf extracts of Piper crocatum contained alkaloid, steroid and tanin. According to this study, it was found that the leaf extract of Piper crocatum can be used to inhibit the growth of B. subtilis and P. aeuruginosa, but can not inhibit the growth of E.coli and S. aureus.
In Silico Analysis of Active Compounds of Avocado Fruit (Persea americana Mill.) as Tyrosinase Enzyme Inhibitors Puspita, Puspa Julistia; Liliyani , Ni Putu Peggy; Ambarsari, Laksmi
Current Biochemistry Vol. 9 No. 2 (2022)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.9.2.3

Abstract

Melanocytes are an important component of skin pigmentation through their ability to produce melanin assisted by the enzyme tyrosinase which is a core enzyme in melanogenesis. The high phenolic and flavonoid compounds in the leaves, seeds and skin of avocados have potential as ligands (inhibitors) of tyrosinase related protein-1. This study aims to determine the active compounds from the leaves, seeds, and skin of avocados that play a role in inhibiting the activity of the tyrosinase enzyme in humans so that it can inhibit the formation of melanin. The research was conducted by molecular anchoring method using YASARA Structure software. The results showed that of the 68 active compounds of avocado leaves, seeds, and skins, there were 11 active compounds that had binding affinity for tyrosinase related protein-1, namely quarcetin, epicatechin, apigenin, 3-Hydroxytyrosol, scopoletin, abscisic acid, citric acid, coumaric acid. , quinic acid, vanillin, and caffeic acid. The active compound abscisic acid is the ligand with the best inhibitory potential with a bond free energy (∆G) of -2,645 kcal/mol which interacts with 17 amino acid residues. The research results can be used as a reference in in vitro and in vivo studies of the human tyrosinase enzyme.
INHIBISI ENZIM α-GLUKOSIDASE OLEH SENYAWA FLAVONOID DAUN KELOR (Moringa oleifera) IN SILICO DAN IN VITRO Puspita, Puspa Julistia; Alimah, Shobiroh Nuur; Ambarsari, Laksmi; Wahyuni, Riksa Nur
Current Biochemistry Vol. 10 No. 2 (2023)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.10.2.3

Abstract

Moringa leaves flavonoid have potential to inhibit α-glucosidase but they have low bioavailability, so they are made in nanoparticles. It is also not known which specific flavonoid compounds from Moringa leaves have the potential to inhibit α-glucosidase. This research aimed to determine the inhibition potential of α-glucosidase by the moringa leaves flavonoid in silico through molecular docking and determine the inhibitory activity of α-glucosidase by moringa leaves flavonoid in extracts and nanoparticles in vitro. Moringa leaves flavonoid have potential to be a competitive inhibiton of α-glucosidase with the highest to lowest inhibitory potential are cryptochlorogenic acid, quercetin-3-O-beta-D-glucopyranoside, quercetin-3-glucoside, kaempferol-3-O-a-ramnoside, kaempferol-3-O-glucoside, epicathechins, catechins, quercetin, kaempferol, glucomoringin isothiocyanate. Cryptochlorogenic acid has the best potential with ΔG and Ki values -8.5 kcal/mol and 0.5788 μM. Inhibition α-glucosidase moringa leaves flavonoid in extract and nanoparticles respectively are classified as inactive (IC 50 = 5.84x10 3 ppm) and active (IC 50 = 1.59x10 1 ppm) in vitro, so nanoparticles can increase the inhibitory activity.
Genetic Diversity of Graptophyllum pictum (L.) Griff from Eastern Indonesia Ethnics Based on Sequence-Related Amplified Polymorphismr Azmi, Wihda Aisarul; Husnawati; Puspita, Puspa Julistia; Safira, Ukhradiya M; Subositi, Dyah; Maruzy, Anshary
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 50 No. 2 (2022): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (638.903 KB) | DOI: 10.24831/jai.v50i2.40644

Abstract

Indonesia berada diposisi kedua dengan jumlah tanaman obat asli tertinggi di dunia setelah Hutan Hujan Amazon. G. pictum (L) Griff merupakan salah satu etnomedisin yang telah dimanfaatkan secara luas oleh masyarakat Indonesia, tapi keragaman genetiknya belum dipetakan. Penelitian ini bertujuan menganalisis keragaman genetik G. pictum (L) Griff dari Indonesia Bagian Timur berdasarkan marka molekuler SRAP melalui pencarian keberadaan polimorfisme. G. pictum (L) Griff yang digunakan berasal dari 11 etnis Indonesia Timur yang sudah dikeringkan sebelumnya, kemudian dilakukan isolasi DNA kromosom, amplifikasi PCR dengan menggunakan 8 kombinasi primer SRAP terpilih, kemudian dianalisis kekerabatan dari 7 etnis menggunakan software NTSYS (Numerical Taxonomy and Multivariative Analysis System) 2.0 dan PopGene (Population Genetic Analysis) 1.3. Dari hasil penelitian diperoleh nilai Persen Lokus Polimorfik (PLP) sebesar 88.55% dengan total lokus ditemukan 49 (44 lokus poliformik dan 5 lokus monomorfik), dan variasi Indonesia menduduki peringkat kedua dengan jumlah tanaman obat asli tertinggi di dunia setelah Hutan Hujan Amazon. Handeuleum (G. pictum (L) Griff) merupakan salah satu tanaman obat yang telah dimanfaatkan secara luas oleh masyarakat Indonesia, namun informasi mengenai keragaman genetiknya masih terbatas. Penelitian ini bertujuan untuk memperoleh informasi mengenai keragaman genetik 52 aksesi G. pictum (L) Griff dari 11 etnis Indonesia Bagian Timur berdasarkan marka molekuler SRAP. Penelitian ini dilaksanakan pada bulan November 2018 hingga Januari 2019. Sebanyak 52 aksesi G. pictum (L) Griff dan 16 kombinasi primer SRAP digunakan dalam penelitian ini. Analisis kekerabatan dilakukan berdasarkan nilai indeks similaritas dan konstruksi dendogram dengan menggunakan program NTSYS 2.0 dan PopGene 1.3. Sebanyak 49 lokus (44 lokus polimorfik dan 5 lokus monomorfik) diperoleh dari delapan kombinasi primer terpilih dengan presentase polimorfik sebesar 88.55%. Keragaman genetik antar aksesi tergolong tinggi (86%) sedangkan antar etnis tergolong rendah (47.23%). Etnis dengan keragaman tertinggi adalah Demta (81.63%), sedangkan Auyu adalah Etnis dengan keragaman genetik yang paling rendah (2.04%). Berdasarkan nilai Indeks Dissimilarity (ID) atau nilai keragaman yang menunjukkan tingkat kekerabatan, Etnis Bajawa dan Auyu memiliki tingkat kekerabatan terjauh (ID = 1.0720). Informasi yang dihasilkan dalam penelitian ini bermanfaat untuk mendukung program pemuliaan dan konservasi daun wungu. Kata kunci: dendogram, handeuleum, SRAP, tanaman obat
Co-Authors Adrian Adiva Alimah, Shobiroh Nuur Azmi, Wihda Aisarul Dyah Subositi Haryo Tejo Prakoso Husnawati Inda Setyawati, Inda LAKSMI AMBARSARI Liliyani , Ni Putu Peggy Maruzy, Anshary Mega Safithri Nirmala Peni Sugiharti Priyono priyono Ranti Selvina Safira, Ukhradiya M Siswanto siswanto Siti Nur'aeni Sri Wahyuni Steffanus Gozales Suryani Suryani Tony Sumaryada Wahyuni, Riksa Nur Waras Nurcholis