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All Journal Pharmacy Reports Annales Bogorienses
Riyona Desvy Pratiwi, Riyona Desvy
Research Center for Biotechnology, Indonesian Institute of Sciences JL. Raya Jakarta - Bogor Km.46 Cibinong 16911 Bogor - Indonesia
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology

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Sensory evaluation of flavoring agent addition in soy-based beverage Pratiwi, Riyona Desvy; Rosyidi, Viddy Agustian; Zanjabilla, Sabighoh; Dewi, Kartika Sari; Novandra, Rio; Desvina, Della; Herawati, Heny
Pharmacy Reports Vol. 2 No. 1 (2022): Pharmacy Reports
Publisher : Indonesian Young Scientist Group and UPN Veteran Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51511/pr.46

Abstract

Soy-based beverages offer numerous health benefits, but the presence of lipoxygenases causes an unpleasant off-flavor sensation that hinders acceptability. The addition of a suitable flavoring agent has been shown to enhance the acceptability of soy-based beverages. This study aimed to investigate preferences towards two flavored variants of soy-based beverages: original chocolate made from cocoa powder and vanilla made from vanilla essence. Before comparing the sensory parameters, which included appearance, taste, odor, texture, and overall assessment, the ratio of basic components such as soy and cow milk powder, sugar, and maltodextrin was studied. Fifty-seven untrained panelists evaluated the three flavored variants of soy-based beverages. The chocolate-flavored soy-based beverage received a significantly higher score in taste, odor, texture, and overall assessment (α = 0.05). Hence, adding cocoa powder as a flavoring agent in soy-based beverages effectively improved the sensory attributes.
Factors Affecting Expression Level of Recombinant Human Epidermal Growth Factor in Escherichia coli BL21(DE3) and Size Exclusion Purification Thereof Pratiwi, Riyona Desvy; Agustina, Nabella; Aminah, Aminah; Fuad, Asrul Muhamad
Annales Bogorienses Vol. 25 No. 2 (2021): Annales Bogorienses
Publisher : BRIN

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Abstract

Recombinant human epidermal growth factor (rhEGF) has been developed to provide the protein for therapeutic uses. Isopropyl β-d-1-thiogalactopyranoside (IPTG)-induced expression in Escherichia coli BL21(DE3) has shown the most effective system among other inducers systems in a similar host. However, suitable conditions related to IPTG concentration, incubation time, and incubation temperature are different depending on the amino acid content of the recombinant protein. This study aimed to evaluate the effects of various IPTG concentration, incubation time, and incubation temperature on rhEGF concentration. According to each analysis of those factors, induction with 0.05 mM IPTG for 2 h at 23°C was the most appropriate condition to obtain the highest concentration of rhEGF. The rhEGF was positively confirmed with a monoclonal antihEGF antibody and purified in high purity reaching 95.2%, yet recovery was low (1.44%) due to loss in fractions containing endogenous proteins. Therefore, further studies related to type of matrix, column length, and sample concentration in applying size exclusion chromatography are requested for higher recovery
Evaluation of Alternative Components in Growth Media of Lactobacillus brevis for Halal Probiotic Preparation Pratiwi, Riyona Desvy; Zanjabila, Sabighoh; Fairuza, Dian; Aminah, Aminah; Praharyawan, Swastika; Fuad, Asrul Muhamad
Annales Bogorienses Vol. 24 No. 1 (2020): Annales Bogorienses
Publisher : BRIN

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Abstract

Probiotic has been widely used in functional food because of numerous advantages for health. MRS broth is commonly used as standard medium in studying lactobacilli. However, in some communities - like muslim and vegetarian society, components in MRS broth/medium become an issue. Beef extract and peptone – animal derived substances as nitrogen sources in the MRS medium should be avoided for the vegetarian. Meanwhile, for the muslim society, all components must be halal-certified including those animal derived ingredients. Therefore, several alternative sources for beef extract and peptone substitution were studied. Combination of alternative nitrogen sources was applied. In order to increase the effect of the alternative nitrogen sources, alternative carbon sources were also included. This is the first report about effects of L. brevis media components on cells growth to expression level of surface layer protein (Slp). Whey, lactose, sucrose, and galactose showed high contribution to L. brevis growth. However, the tested concentration of those substances were not sufficient to obtain equal bacterial growth and Slp expression than that of MRS broth. In addition, yeast extract appeared necessary to maintain cell wall and Slp expression.
A Preliminary Report on The Syntheses of Oligonucleotide Primers in The National Research and Innovation Agency (NRIA) Atikana, Akhirta; Prasetyoputri, Anggia; Rubiyana, Yana; Herawati, Neng; Desriani, Desriani; Pratiwi, Riyona Desvy; Wulandari, Dwi; Sukmarini, Linda; Kusharyoto, Wien; Santoso, Adi; Putra, Masteria Yunovilsa; Lisdiyanti, Puspita
Annales Bogorienses Vol. 26 No. 1 (2022): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2022.v26.n1.21-27

Abstract

A PolyGen DNA Synthesizer is equipment that is used for synthesizing oligonucleotide primers for any amplification targets. Oligonucleotide primers are indispensable components for any Polymerase Chain Reaction (PCR)-based detections. In the present study a number of oligonucleotide primer sets were synthesized to target (1) the Human Insuline Glargin (HIG) and (2) the Human Erythropoietin (EPO), as well as (3) the RNA-dependent RNA Polymerase (RdRp) and (4) the Nucleocapsid (N) genes of the severe acute respiratory syndrome virus 2 (SARS-CoV-2). A solid-phase oligonucleotide synthesis method was used according to the default protocol of the Polygen’s instrument to synthesize primers at a 40 nmol scale. The synthesized primers in this study were compared to commercially produced primers in their ability to amplify the gene target(s) in PCR and quantitative real-time PCR (qPCR) reactions. The first two sets of primers showed similar results in PCR compared to commercial primers; however, these primers were not tested for qPCR due to sample limitation. In contrast, the primer sets 3 and 4 were not able to produce amplicons in PCR reactions and only the primer set 4 successfully amplified the gene target in qPCR. These results indicate that the crude primers synthesized in this study are promising candidates for molecular detection and diagnostics, but these primers would benefit from further optimization for routine applications.
Co-Authors Adi Santoso Agustina, Nabella Aminah Aminah Asrul Muhamad Fuad, Asrul Muhamad Atikana, Akhirta Desriani Desriani Desvina, Della Dwi Wulandari Fairuza, Dian Heny Herawati Kartika Sari Dewi Kusharyoto, Wien LINDA SUKMARINI Neng Herawati, Neng Novandra, Rio Prasetyoputri, Anggia PUSPITA LISDIYANTI Putra, Masteria Yunovilsa Swastika Praharyawan, Swastika Viddy Agustian Rosyidi Yana Rubiyana, Yana Zanjabila, Sabighoh Zanjabilla, Sabighoh