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OPTIMASI EFISIENSI FLOKULASI PADA PROSES PANEN MIKROALGA POTENSIAL PENGHASIL BIODIESEL DENGAN FLOKULAN ION MAGNESIUM - (OPTIMIZATION OF FLOCCULATION EFFICIENCY IN THE HARVESTING PROCESS OF POTENTIAL BIODIESEL PRODUCING MICROALGAE BY USING MAGNESIUM IONS) Praharyawan, Swastika; Putri, Silviredeta Anindya
Biopropal Industri Vol 8, No 2 (2017)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.105 KB)

Flocculation is a feasible method for harvesting microalgae due to its lower cost. This research aims to optimize the flocculation efficiency in harvesting biodiesel-producing microalgae. Flocculation process was conducted towards microalgae cell suspension on 0.2-1 g/L range concentration by applying magnesium ions (1 until 5 mM) at alkaline condition (pH 9-12) in 30 and 60 minutes using neutralization and sweeping. The result showed that magnesium ion concentration, pH value and biomass concentration had significant effect on the efficiency of microalgae cells flocculation, while flocculation time did not show significant effect. Flocculation efficiency increased when magnesium ion concentration was 4 mM and started at pH 10 for microalgae culture with high biomass concentration and at pH 11.5 for low biomass concentration, while optimum pH for both culture was 11.75. Flocculation efficiency for microalgae with high concentration of biomass at optimum condition was 94.89% while for the low one was 89.75%.Keywords: flocculation, flocculation efficiency, magnesium ions, microalgaeABSTRAKMikroalga berpotensi sebagai bahan baku biodiesel. Metode flokulasi layak untuk diterapkan pada pemanenan mikroalga karena berbiaya rendah. Penelitian ini bertujuan untuk mengoptimasi efisiensi flokulasi pada proses pemanenan mikroalga lokal potensial penghasil biodiesel yang menggunakan metode otoflokulasi di bawah pengaruh nilai pH, konsentrasi ion magnesium, konsentrasi biomassa dan waktu flokulasi. Proses flokulasi dilakukan pada suspensi sel mikroalga konsentrasi 0,2 hingga 1 g/L dengan menggunakan ion magnesium (1 hingga 5 mM) pada suasana basa (pH 9-12) selama waktu tertentu (30 dan 60 menit) melalui mekanisme netralisasi muatan dan penyapuan (sweeping). Hasil penelitian menunjukkan bahwa konsentrasi ion magnesium, nilai pH dan konsentrasi biomassa memberikan pengaruh yang signifikan terhadap efisiensi flokulasi sel-sel mikroalga sedangkan waktu flokulasi tidak menunjukkan pengaruh signifikan. Efisiensi flokulasi meningkat secara signifikan pada konsentrasi ion magnesium hingga 4 mM dan dimulai pada pH 10 untuk kultur mikroalga dengan konsentrasi biomassa tinggi (1 g/L) dan pada pH 11,5 untuk kultur dengan konsentrasi biomassa rendah (0,2 g/L) sedangkan pH optimum untuk kedua kultur adalah 11,75. Nilai efisiensi flokulasi untuk kultur mikroalga dengan konsentrasi biomassa tinggi pada kondisi optimum mencapai 94,89%, sementara nilai efisiensi flokulasi untuk kultur dengan konsentrasi biomassa rendah adalah 89,75%.Kata kunci: efisiensi flokulasi, flokulasi, ion magnesium, mikroalga

Morphology and molecular characterization of newly isolated microalgae strain Chlorella volutis LIPI13-WKT066 from Wakatobi Islands and its potential use Rahman, Delicia Yunita; Praharyawan, Swastika; Raharjo, Sapto; Fadiyah, Farizul; Susilaningsih, Dwi
ANNALES BOGORIENSES Vol 23, No 1 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2019.v23.n1.13-19

Morphology and molecular characterization of microalgae isolated from Wakatobi Marine National Park was conducted. An understanding of the characteristics of morphology, molecular, as well as metabolites profile of the microalgae species is potentially useful for its future application. The primary aim of this study was to isolate, identify and characterize the microalgae strain isolated from Wakatobi Marine National Park labeled as LIPI13-WKT066 with the emphasis on the evaluation of amino acid content as a basis for its health application. Morphological observation under the microscope and molecular identification suggested that the microalgae strain of LIPI13-WKT066 belong to the strain under species of Chlorella volutis. Metabolite characterization of the microalgae strain showed that the content of protein (11.9%), lipid (12.4%) and carbohydrate (4.7%) was in the regular range. Further analysis of its amino acid content revealed the potency of the microalgae strain to be used as antihypertensive agent.

Characterization of lipid productivity and fatty acid profile of three fast-growing microalgae isolated from Bengkulu for possible use in health application Praharyawan, Swastika; Rahman, Delicia Yunita; Susilaningsih, Dwi
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.03

Three strains of fast-growing microalgae were investigated in this study for their potential of lipid production and its possible use in health application. Lipid content, lipid productivity, and fatty acids profile of the 3 mi-croalgae strains were also analyzed. The strain of LBB13-2-AL045 and LBB13-2-AL048 possessed highest lipid content (49.08 Â± 0.25%) and lipid productivity (40.27 Â± 1.91 mg.L-1.day-1), respectively, among the other tested strains. The fatty acids profile from the 3 strains exhibited its possible use in health application. The two strains of LBB13-2-AL046 and LBB13-2-AL048 possessed high valuable fatty acids of polyunsaturated fatty ac-ids (PUFA) omega-3 and omega-6, whereas LBB13-2- AL045 possessed balance ratio of saturated fatty acid (SFA) : monounsaturated fatty acid (MUFA) : PUFA (1 : 1.3 : 1) as is recommended by American Heart As-sociation (AHA) (1 : 1.4 : 0.8). The high content of omega-3 and omega-6 fatty acids indicated that the algal lipids of two strains (LBB13-2-AL046 and LBB13-2-AL048) were potential to be applied in cardiovascular health. The balance ratio of SFA : MUFA: PUFA as is recommended by AHA indicated that the algal oil of LBB13-2-AL045 strain was recommended in order to generate the best LDL/HDL ratio.

Effect of Light Quality, Light Intensity, and Cell Inoculum Arrangement on Growth, Pigment and Carbon Content from Spirulina platensis using LED Light Dianursanti; Chempaka, Rayi Mishellia; Hartono, Nelvina; Habibah, Lulu; Praharyawan, Swastika
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1123-1134

This study evaluates the effect of lighting and cell inoculum in Spirulina platensis cultivation on its growth, pigment composition, and ability to capture CO2. Different light intensities (1,000, 3,000, and 5,000 lux), light quality (white, blue, and red), and cell inoculum which are shown as OD values (0.2, 0.3, and 0.5) were assessed. The highest growth rate was obtained from red light, followed by white and blue light. The maximum biomass concentration (0.0711 mg/ml) was obtained when red light was used under 5,000 lux light intensity. The highest carbon content (5.1274 mg/ml algae) was also obtained during red light cultivation under 5,000 lux light intensity. Meanwhile, the highest chlorophyll (1.4365 mg/mg algae) content was obtained at blue light cultivation under 5,000 lux intensity and OD 0.5 cell inoculum. The highest phycocyanin (0.0309 mg/mg algae) was obtained under red light with 3,000 lux light intensity and OD 0.5 cell inoculum. It was found that the cultivation conditions to achieve high biomass and high pigment concentration were different.

Phycocyanin production from Galdieria sulphuraria 009 in palm oil mill effluent: growth, extraction, and antioxidant activity: Produksi fikosianin dari Galdieria sulphuraria 009 dalam limbah cair pabrik kelapa sawit: pertumbuhan, ekstraksi, dan aktivitas antioksidan Rahman, Delicia Yunita; Praharyawan, Swastika; Apriastini, Marsiti; Nurcahyani, Puji Rahmawati; Nafisyah, Ayu Lana; Fatriasari, Widya; Amrullah, Apip; Farobie, Obie
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 28 No. 5 (2025): Jurnal Pengolahan Hasil Perikanan Indonesia 28(5)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v28i5.63115

Limbah cair industri kelapa sawit (palm oil mill effluent atau POME) di Indonesia menghasilkan volume yang tinggi dan berpotensi mencemari lingkungan. Mikroalga dapat dimanfaatkan untuk mengurangi limbah sekaligus menghasilkan produk biomassa yang bernilai tambah. Penelitian ini bertujuan menentukan konsentrasi optimum POME bagi pertumbuhan mikroalga Galdieria sulphuraria 009, mengevaluasi produksi fikosianin, serta menilai aktivitas antioksidannya. Penelitian ini diawali dengan penapisan awal menggunakan 5–50% POME untuk mengidentifikasi kondisi pertumbuhan mikroalga yang optimum; budi daya dalam bioreaktor dengan variasi konsentrasi (2,5; 5,0; dan 7,5%) untuk mengevaluasi kinerja pertumbuhan; dan analisis aktivitas antioksidan serta kandungan pigmen dalam biomassa. Penapisan awal menunjukkan bahwa POME 5% merupakan konsentrasi optimal, sedangkan konsentrasi lebih tinggi menghambat pertumbuhan akibat berkurangnya penetrasi cahaya. Pada budidaya lanjutan, mikroalga dalam POME 2,5% menunjukkan hasil sebanding dengan kontrol (Allen pH 2), sedangkan konsentrasi lebih tinggi menghambat pertumbuhan akibat penaungan dan toksisitas amonia. Produksi fikosianin per volume kultur tertinggi diamati pada mikroalga yang dibudidayakan dalam POME 2,5%, dengan hasil yang sebanding dengan media kontrol. Uji aktivitas antioksidan mengonfirmasi bahwa semua ekstrak fikosianin memiliki aktivitas antioksidan yang signifikan, dengan aktivitas tertinggi pada POME 7,5%. Kandungan karotenoid dan klorofil a dievaluasi dalam biomassa segar dan residu setelah ekstraksi. Karotenoid lebih melimpah dalam biomassa segar, sedangkan klorofil a lebih tinggi dalam residu biomassa. Penelitian ini menunjukkan bahwa G. sulphuraria 009 berpotensi sebagai sumber fikosianin yang layak dalam budidaya berbasis POME, serta memberikan wawasan mengenai pemanfaatan limbah industri dan pengembangan produk bioteknologi berkelanjutan.

Evaluation of Alternative Components in Growth Media of Lactobacillus brevis for Halal Probiotic Preparation Pratiwi, Riyona Desvy; Zanjabila, Sabighoh; Fairuza, Dian; Aminah, Aminah; Praharyawan, Swastika; Fuad, Asrul Muhamad
Annales Bogorienses Vol. 24 No. 1 (2020): Annales Bogorienses
Publisher : BRIN

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Probiotic has been widely used in functional food because of numerous advantages for health. MRS broth is commonly used as standard medium in studying lactobacilli. However, in some communities - like muslim and vegetarian society, components in MRS broth/medium become an issue. Beef extract and peptone – animal derived substances as nitrogen sources in the MRS medium should be avoided for the vegetarian. Meanwhile, for the muslim society, all components must be halal-certified including those animal derived ingredients. Therefore, several alternative sources for beef extract and peptone substitution were studied. Combination of alternative nitrogen sources was applied. In order to increase the effect of the alternative nitrogen sources, alternative carbon sources were also included. This is the first report about effects of L. brevis media components on cells growth to expression level of surface layer protein (Slp). Whey, lactose, sucrose, and galactose showed high contribution to L. brevis growth. However, the tested concentration of those substances were not sufficient to obtain equal bacterial growth and Slp expression than that of MRS broth. In addition, yeast extract appeared necessary to maintain cell wall and Slp expression.

Morphology and Molecular Characterization of Newly Isolated Microalgae Strain Chlorella volutis LIPI13-WKT066 from Wakatobi Islands and Its Potential Use Rahman, Delicia Yunita; Praharyawan, Swastika; Fadiyah, Farizul; Raharjo, Sapto; Susilaningsih, Dwi
Annales Bogorienses Vol. 23 No. 1 (2019): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Morphology and molecular characterization of microalgae isolated from Wakatobi Marine National Park was conducted. An understanding of the characteristics of morphology, molecular, as well as metabolites profile of the microalgae species is potentially useful for its future application. The primary aim of this study was to isolate, identify and characterize the microalgae strain isolated from Wakatobi Marine National Park labeled as LIPI13-WKT066 with the emphasis on the evaluation of amino acid content as a basis for its health application. Morphological observation under the microscope and molecular identification suggested that the microalgae strain of LIPI13-WKT066 belong to the strain under species of Chlorella volutis. Metabolite characterization of the microalgae strain showed that the content of protein (11.9%), lipid (12.4%) and carbohydrate (4.7%) was in the regular range. Further analysis of its amino acid content revealed the potency of the microalgae strain to be used as antihypertensive agent.

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