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All Journal HAYATI Journal of Biosciences
Totik Sri Mariani
School of Life Sciences and Technology, Institut Teknologi Bandung (ITB), Bandung, Indonesia
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Effect of Media Variation on the Induction and Phytochemical Profile of Callus in Two Varieties of Cat's Whiskers (Orthosiphon aristatus Blume Miq) Fahrauk Faramayuda; Akhirul Kahfi Syam; Totik Sri Mariani; Elfahmi; Sukrasno
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.159-170

Abstract

The levels of rosmarinic acid and sinensetin in purple and white-purple varieties of Orthosiphon aristatus, cat's whiskers, can be increased using modified in vitro culture. This work focused on callus induction of the purple and white-purple variety of cat's whiskers grown on Gamborg (B5) and CHU (N6) with the addition of growth regulators 2,4-dichlorophenoxyacetis acid. Our observation suggested that the callus could grow within three weeks and produce rosmarinic acid and sinensetin. The level of sinensetin from various extraction methods is relatively low; in contrast, the rosmarinic acid from purple callus was detected at about 5% w/w, while the white-purple variety was around 2% w/w. The results of this study also provided new information on the basic media other than MS that can grow cat's whiskers callus while producing active compounds.
Co-Authors Akhirul Kahfi Syam Elfahmi Elfahmi, Elfahmi Fahrauk Faramayuda Sukrasno