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All Journal Jurnal Sain Veteriner Techno-Fish
Wahyu Nurlita
Balai Karantina Ikan, Pengendalian Mutu dan Keamanan Hasil Perikanan Denpasar, Kementerian Kelautan dan Perikanan
Agriculture, Biological Sciences & Forestry Veterinary

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Infection of Infectious Spleen and Kidney Necrosis Virus and Viral Nervouse Necrosis in Seawater Fish Identifed Using Multiplex Polymerase Chain Reaction Hidayati Kumalasari; Putu Eka Sudaryatma; Artanti Tri Lestari; Wahyu Nurlita; Wahyu Andy Nugraha; Nur Hasanah; Ida Ayu Mirah Meliana Dewi; Ni Putu Arya Shintya Anggraeni
Jurnal Sain Veteriner Vol 40, No 2 (2022): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.73599

Abstract

Indonesia become a maritime country with large of ocean area and beach line with marine species diversity reaches 37% of the world’s fish. This makes Indonesia have aquaculture potential that supported by an appropriate climate. However, the challenge of viral diseases caused by Infectious Spleen and Kidney Necrosis Virus (ISKNV) and Viral Nervous Necrosis (VNN) would hamper marine aquaculture and causing huge economic losses. Therefore, it is necessary to carry out early detection methods that are efficient, fast, precise and accurate to identifying these viral disease. Here we developed a multiplex-PCR (mPCR) a method that can detect simultaneously of the ISKNV and VNN. The results of this method showed high sensitivity and specificity by using MCP and CP gene target primers to detect ISKNV and VNN, respectively. From a total of 353 samples of seawater fish examined by mPCR, positive results following of single infection of ISKNV and VNN were 14 and 2, respectively. We also found that 18 grouper fish was co-infection with these viruses. From these results, it shows that the mPCR method developed has efficiency, faster and high accuracy. This founding was possible to be applied in laboratory testing or early detection system in the field for ISKNV and VNN. 
DETEKSI MOLEKULER PENYAKIT VIRAL NERVOUS NECROSIS (VNN) PADA IKAN KERAPU DI WILAYAH KABUPATEN BULELENG PROVINSI BALI Rio Aditya Kurniawan; Budi Sugianti; Ni Desak Nyoman Pradnyani; Artanti Tri Lestari; Anwar; I Nyoman Suardana; Wahyu Nurlita; Imanuddin Razak
Techno-Fish Vol 7 No 1 (2023): TECHNO-FISH
Publisher : TECHNO-FISH

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25139/tf.v7i1.6316

Abstract

Viral Nervous Necrosis (VNN) disease is one of the diseases included in the list of aquatic animal diseases of the World Organization for Animal Health (WOAH) and is a pathogen that poses a threat to grouper aquaculture throughout the world. VNN infection can occur vertically and horizontally and has a mortality rate of up to 100%. This study aimed to detect VNN using molecular methods (PCR) in grouper cultured in Buleleng, Bali. The method used is molecularly by conventional PCR. The results of this study indicated that groupers with VNN showed rotating, solitary swimming and enlarged eyelets (exophthalmos). The internal organs show enlargement of the spleen and kidneys. The liver looks pale and haemorrhage occurs in other internal organs. The results of the PCR examination showed 5 (five) positive samples of VNN with the appearance of a band at 230 bp so that the prevalence of VNN was 25%. From the results of this study, recommendations that can be taken are monitoring and surveillance of VNN need to be carried out continuously in grouper aquaculture, this is necessary to determine actions to be taken quickly if VNN is detected in an aquaculture unit. Keywords: betanodavirus grouper, molecular, PCR, VNN
Co-Authors Anwar Artanti Tri Lestari Artanti Tri Lestari Budi Sugianti Hidayati Kumalasari I Nyoman Suardana Ida Ayu Mirah Meliana Dewi Imanuddin Razak Ni Desak Nyoman Pradnyani Ni Putu Arya Shintya Anggraeni Nur Hasanah Putu Eka Sudaryatma Rio Aditya Kurniawan Wahyu Andy Nugraha