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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya Journal of Community Health and Preventive Medicine (JOCHAPM)
Rivo Yudhinata Brian Nugraha
Laboratory Of Parasitology Faculty Of Medicine Universitas Brawijaya Malang, Indonesia
Agriculture, Biological Sciences & Forestry Environmental Science Health Professions Medicine & Pharmacology Nursing Public Health

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Growth Rate Differences of Chrysomya sp. Larvae on Rattus novergicus Wistar Strain Corpse Exposed and Unexposed to Ephedrine Toxic Dose Sri Poeranto; Wening Prastowo; Diandra Laksmita Resmi; Rivo Yudhinata Brian Nugraha
Journal of Tropical Life Science Vol. 7 No. 3 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.03.06

Abstract

Post Mortem Interval (PMI) is used as a parameter to define the onset, cause, manner, and time of death to help maintenance of law and identify crime victims by the age of the larvae found. Larval growth is influenced by the temperature, humidity, and contaminant (drug or toxic). This experiment used two media of dead rats (200 grams), one given ephedrine of LD50 = 266 mg/kg and the other without ephedrine. Both were put in 2 cages each containing 50 Chrysomya sp. Ten larvae were randomly taken every day for 14 days to be measured their length, weight, and duration of growth. The 3rd larvae stage in the media with ephedrine had weight gain on 5th – 6th day morning, while larvae in media without ephedrine had static weight gain on 5th day morning - afternoon and weight decrement on 6 – 7th day morning. Larva’s peak ratio of length/weight in the media with ephedrine was higher than that of larvae in the media without ephedrine. Larvae on media with ephedrine grew faster in 1st, 2nd, 3rd larvae stage, and pupal stage compared to larvae without ephedrine. Therefore, this study indicates that ephedrine can accelerate the growth rate of Chrysomya sp. larvae.
The Use of Natural Phenomenon in Obtaining Pregnant Rats and Mice as Experimental Animals with The Same Gestational Ages Teguh Wahju Sardjono; Harry Kurniawan Gondo; Rivo Yudhinata Brian Nugraha; Anisa Tiara Putri; Meyland Citra Oktri Sienty Effendy
Journal of Tropical Life Science Vol. 9 No. 3 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Hormonal oestrus synchronization is often used to obtain the same gestational ages animal models, but it may not physiologic nor practical. This research was conducted to find out a simple method in obtaining physiologic pregnant experimental animals. We used 120 female rats and mice each, divided into 16 groups with 15 members each. They were classified according to the state of parities, pair or group mating, and synchronization of oestrous. The estrous synchronization is done by utilizing the natural phenomenon (Lee-Boot, Pheromone, and Whitten effect). All nulliparous and multiparous models then were simultaneously mated for one night in pairs (1:1) or in groups (1:5) subsequently. Pregnancy rates were noted based on the number of models delivering pups on day 19-23 post-mating. The highest pregnancy rate was noted in group of multiparous, synchronized and monogamous rats (86.7%), followed by nulliparous, synchronized and monogamous rats (73.3%) and multiparous, synchronized and monogamous mice (60%) (p<0.05). Synchronization of the estrous and monogamous mating of rats or mice had significant roles in pregnancy rates (p<0.05). To get a high number of pregnant rats or mice models, we suggest to use the multiparous, estrous synchronized and monogamous simultaneous mating within one night.
Optimation of Combination of N-Hexane Solution and Ethyle Acetate on Secondary Metabolite Compounds Profile of Streptomyces hygroscopicus Dio Giovanni Ariel; Sri Winarsih; Fitria Febriliani Putri; Nabila Erina Erwan; Ajeng Maharani Putri; Alfian Wika Cahyono; Kana Mardhiyyah; Loeki Enggar Fitri; Rivo Yudhinata Brian Nugraha
Jurnal Kedokteran Brawijaya Vol. 31 No. 3 (2021)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2021.031.03.11

Abstract

Streptomyces hygroscopicus (S.hygroscopicus) is a Gram-positive soil bacterium that can produce secondary metabolites from fermentation that have a therapeutic effect. The fermented S. hygrocospicus metabolites that are still in the form of crude extracts are difficult to develop as drug preparations because the active compounds are not yet known, so it will be challenging to determine the dosage of drugs that have a therapeutic effect. Therefore, it is necessary to carry out exploratory research to narrow down the secondary metabolite profile from the fermentation of S. hygroscopicus, using extraction and fractionation methods, which are then identified by Thin-Layer Chromatography (TLC) using a combination of solvents. This study used the extraction method with a separating funnel. The fractionation was carried out using the BUCHI (Sepacore®) Flash Chromatography and Reveleris® PREP Purification System column chromatography gradually using ethyl acetate and n-hexana.  47 and 60 of the fractionation results were taken as samples, that further were profiled using TLC and given the appearance of 10% KOH stains and p-Anisaldehyde - sulfuric acid, so that various classes of compounds with different Rf values were obtained, namely Monoterpenes, Triterpenes, Steroids, Saponins, Coumarin, Scopoletin, and Alkaloids.
COMMUNITY EMPOWERMENT REGARDING “ONE HOUSE ONE JUMANTIK” PROGRAM IN KARANG BESUKI SUB-DISTRICT, MALANG CITY Alidha Nur Rakhmani; Lilik Zuhriyah; Rivo Yudhinata Brian Nugraha
Journal of Community Health and Preventive Medicine Vol. 1 No. 1 (2021): JOCHAPM Vol. 1 No. 1 2021
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (213.165 KB) | DOI: 10.21776/ub.jochapm.2021.001.01.4

Abstract

In 2016, Indonesia has been launching the “Indonesia Sehat'' program which the strategy used is to strengthen health services through a family approach. According to the 2016 Ministry of Health technical guidelines, the larva inspector, also known as Juru Pemantau Jentik (Jumantik), is involved in Jumantik participating by each family. Their role is the same as 3M plus actions including in the inspection, monitoring, and eradication of mosquito larvae for controlling infectious diseases, especially Dengue Fever. The purpose of these activities was to increase community participation to conduct a “one house one Jumantik'' program. The study was the quasi-experimental post-test only design that was located in Karangbesuki sub-district, Malang City from August until September 2017. One cluster Rukun Warga (RW) from the Karangbesuki sub-district that consisted of 9 Rukun Tetangga (RT) was chosen as the location. The intervention was in collaboration with Mulyorejo Public Health Center (Pusat Kesehatan Masyarakat / Puskesmas) in Malang. The methods used were ovitrap utilization training and focus group discussion (FGD), health promotion and "one-house one-Jumantik'' training program, also the contest to choose the best RT. The results of this activity succeeded in establishing a Jumantik team in each RT although there was one RT that has not yet succeeded in forming a team. Furthermore, for the sustainability of the program, it is hoped that this program could be improved by collaborating with Mulyorejo Puskesmas staff who monitor activities routinely through the integrated service post (Pos Pelayanan Terpadu / Posyandu).
Co-Authors Ajeng Maharani Putri Alfian Wika Cahyono Anisa Tiara Putri Diandra Laksmita Resmi Dio Giovanni Ariel Fitria Febriliani Putri Harry Kurniawan Gondo Kana Mardhiyyah Lilik Zuhriyah Loeki Enggar Fitri Meyland Citra Oktri Sienty Effendy Nabila Erina Erwan Rakhmani, Alidha Nur Sri Poeranto Sri Winarsih Teguh Wahju Sardjono Wening Prastowo