Ida Bagus Kade Suardana
Laboratorium Virologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia

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Titer Antibodi Newcastle Disease pada Broiler yang Divaksin Umur Satu hari dan Dibooster Umur 15 hari Ni Made Suksmadewi Wisnantari; Ida Bagus Kade Suardana; Tjokorda Sari Nindhia
Buletin Veteriner Udayana Vol. 14 No. 6 December 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i06.p07

Abstract

Newcastle Disease is one of the major infectious diseases that often attacks broiler farms. The objective of the study was to find out the number of Newcastle Disease antibody titers of broiler vaccinated at the age of a day in nursery and given booster in 15 days. The research sample was 20 broiler DOC divided into 2 groups and each group contained of 10 DOC. The control group (P0) was unvaccinated DOC of broiler and the group (P1) was vaccinated DOC of broiler at the age of a day in nursery and given booster in 15 days age. Blood samplings were conducted on the 7th, 14th, 21st, and 28th days in each group. Newcastle Disease antibody titer examination was done using Haemaglutination Inhibition. The antibody titer value was then analyzed using variance (Anova) and regression test. The results showed that booster vaccination performed at 15 days of age showed a protective antibody titer at 21 days of age, namely 5.8 HI log 2 (? 4 HI log 2) and increased up to 28 days of age, namely 6.5 HI log 2 (? 4 HI log 2). The conclusion of this study is that the Newcastle Disease broiler antibody titer increased after booster vaccination was performed at 15 days of age. Suggestions need to be given a booster vaccination in broilers vaccinated at day one.
Deteksi Antibodi Maternal Newcastle Disease pada Broiler Ida Bagus Ketut Indra Permana; Ida Bagus Kade Suardana; Tjokorda Sari Nindhia
Buletin Veteriner Udayana Vol. 15 No. 1 February 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v01.i01.p15

Abstract

Nevertheless, it is often found that the after vaccination antibody titers is not as expected due to the existance of maternal antibodies. A passive maternal antibodies can inhibit formation of imunoglobulin and neutralize the antigen vaccines. According to that above mentioned explanation, this research aims to detect the maternal antibodies of Newcastle Disease in broilers to carefully identify when the chicken should be vaccinated. As the main object of study, 10 unvaccinated broilers would be included in this research. These samples would be grouped by 3 different times of collecting blood, namely at the age of 7th, 14th, and 21st days. Blood sampling is subsequently carried out through branchialis vein that concludes 10 serum samples from each day. The respective samples will be serologically examined by conducting the haemaglutination (HA) and haemaglutination inhibition (HI) analysis to generate the antibody titers. All the data produced in this research will statistically processed by using descriptive statistical and regression tests on the SPSS software. The results showed a decrease in the maternal antibody titer of Newcastle Disease in broilers aged 7 days with a titer of 6.1 HI log 2, to 2.8 HI log 2 at 14 days of age, and 1.8 HI log 2 at 21 days of age. The Newcastle Disease vaccination program is most appropriate when the broiler is 10 days old because the maternal antibody titer is already at the positive threshold, namely 4 HI log 2.
Perbedaan Titer Antibodi Newcastle Disease pada Broiler yang Divaksinasi Umur Satu Hari dan Tujuh Hari Dwi Arum Permatasari; Ida Bagus Kade Suardana; Tjokorda Sari Nindhia
Buletin Veteriner Udayana Vol. 15 No. 1 February 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v01.i01.p16

Abstract

Newcastle Disease (ND) is a disease caused by avian Paramyxovirus type-1. Common symptoms had seen early in infection are decreased appetite, lethargy and conjunctivitis. The main strategy that can be done to prevent the emergence of ND disease is by implementing a biosecurity system and vaccination, the success of vaccination is supported by post-vaccination antibody titer examination. This study aims to compare the determination of Newcastle Disease antibody titers in broiler vaccinated at the age of one day at the factory and seven days old.This study used 30 broiler divided into three treatment groups. The first sample chickens group were not vaccinated or known as control samples (P0). The second group of chickens has vaccinated one-day ages at the factory (P1). The third group of vaccinated chickens was seventh days old (P2). Blood sampling in the P0 group was carried out starting at the age of 7, 14, 21, 28 and 35 days, the P1 group at the age of 7, 14 and 21 days and in the P2 group at the age of 14, 21 and 28 days at random (random sampling) through the brachial vein. The examination of serum antibody titers can be using the Haemagglutination Inhibition (HI) serological test. The data obtained from the serological examination results were analyzed using the statistical test of variance and regression analysis test SPSS software. The results showed that there was a significant difference in ND antibody titers in the first and second weeks after vaccination and there was no significant difference in the third week after vaccination. It is necessary to booster broilers vaccinated one day at the age of 13 days and broilers vaccinated at seven days at the age of 25 days to increase immunity again.