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All Journal Buletin Veteriner Udayana
Ida Bagus Kade Suardana
Laboratorium Virologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia
Immunology & microbiology Public Health Veterinary

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THE INFLUENCE OF ADMINISTERING MENIRAN EXTRACT FOR SEVEN DAYS ON BROILERS BEFORE NEWCASTLE DISEASE VACCINATION I Made Indra Palaguna; Ida Bagus Kade Suardana; Gusti Ayu Yuniati Kencana
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2024 Vol. 16 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i04.p37

Abstract

Newcastle disease (ND) is a detrimental viral disease that affects broiler chickens. Chickens that contract the virus post-vaccination cause significant losses for farmers. Administering meniran extract is one alternative that can enhance the effectiveness of vaccination in chickens. This study aims to determine the effect of administering meniran extract for 7 days before vaccination on the antibody titer against Newcastle disease. The research subjects used were 30 broiler DOCs (Day-Old Chicks) aged 1 day to 30 days. The research design employed a Completely Randomized Design with 3 treatments. The research procedures included preparation of the cages, animal treatment, vaccination, blood sampling, and ND antibody titer testing. Data analysis was performed using ANOVA and Regression Analysis. The study showed an increase in the average antibody titer in each treatment group. The results of this study indicate that administering meniran extract can increase the antibody titer in chickens vaccinated with the LaSota (active) vaccine in the first week before vaccination, as seen in the average antibody titer value of the treatment group, which increased up to 4.5 HI units log 2. Based on the results of the study, further research is needed with a longer administration period of meniran extract or different doses to observe the increase in antibody titer.
POTENTIAL OF MENIRAN LEAF EXTRACT ON NEWCASTLE DISEASE ANTIBODY TITER IN BROILERS Citra Widiawati; Ida Bagus Kade Suardana; Gusti Ayu Yuniati Kencana; Ni Luh Eka Setiasih; Tjokorda Sari Nindhia; Anak Agung Sagung Kendran
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p15

Abstract

Newcastle Disease (ND) is one of the infectious diseases that easily attack broilers. Vaccinations carried out to optimize chicken immunity often get unsatisfactory results, so green meniran (Phyllanthus niruri Linn.) is needed as an immunostimulator. Green meniran leaf extract contains flavonoid compounds as the main component to trigger T cells to help B cells to produce antibodies. This study aims to determine the effect of meniran leaf extract (Phyllanthus niruri Linn.) on booster vaccinated broilers on ND antibody titer. The research design used was a complete randomized design in a nested pattern with a total of 30 broilers divided into three treatment groups, namely 10 broilers not given the ND La Sota booster vaccine and not given meniran leaf extract but given a placebo (K-), 10 broilers given the ND La Sota booster vaccine without meniran leaf extract (K+), and 10 broilers given the ND La Sota booster vaccine and meniran leaf extract in drinking water for seven days before and 14 days after vaccination (P). The samples used were one day before booster vaccination (9-day-old broilers), one week after booster vaccination (17-day-old broilers) and two weeks after booster vaccination (24-day-old broilers). Serum obtained was examined serologically by hemagglutination inhibition (HI) test. The data obtained were analyzed statistically Analysis of Variance (Anova) with a significant level of 5% and regression analysis using SPSS (Statistical Product and Service Solution) software. The results showed that in the administration of meniran leaf extract(Phyllanthus niruri Linn.) and the effect of sampling time after ND booster vaccination had an increase in ND antibody titer which was significantly different (P < 0.05) in treatment P with a mean of 2.8; 4.3; 7.1 and a total mean of 4.7 (titer in HI log 2). Regression analysis showed that the P treatment had the highest increase in the third week after ND booster vaccination compared to the K- and K+ treatments.
HETEROPHIL-LYMPHOCYTE RATIO IN LAYING HENS VACCINATED WITH NEWCASTLE DISEASE-INFECTIOUS BRONCHITIS Savira Hadi Putri; Anak Agung Sagung Kendran; Gusti Ayu Yuniati Kencana; I Made Damriyasa; Ida Bagus Kade Suardana; I Nyoman Sulabda
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2024 Vol. 16 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i04.p29

Abstract

Vaccination plays a role in the formation of specific immunity against certain antigens. Stressful conditions in animals can potentially influence post-vaccination immune responses. To be able to monitor this condition, it can be seen using stress indicators in poultry, namely the heterophil-lymphocyte ratio (H/L) value. This research was conducted to determine the value of the heterophil-lymphocyte ratio (H/L) in laying hens vaccinated with ND-IB. The samples used were 10 chicken blood samples taken from 40 chickens randomly during the pre-vaccination period; one week, two weeks, and three weeks post-vaccination. The research was preceded by calculating total leukocytes and leukocyte differential, followed by calculating the heterophil-lymphocyte (H/L) ratio value from the results obtained, then analyzed using the SPSS program. The results of this research show that livestock experienced mild stress in the first week post-vaccination which is thought to have occurred due to the handling carried out. A significant increase in the heterophil-lymphocyte ratio (H/L) value occurred in the second week post-vaccination, this occurred due to the influence of environmental factors in the form of bacterial infections. In the third week post-vaccination, livestock no longer experience stress. Further research needs to be carried out using negative controls to determine the exact cause of the increase in the heterophil-lymphocyte (H/L) ratio.
PATHOGENIC ESCHERICHIA COLI BACTERIA INFECTION IN LANDRACE PIGLETS IN ANTIGA KELOD, MANGGIS, KARANGASEM Yolla Noviolita; I Gusti Ketut Suarjana; Ida Bagus Oka Winaya; Ida Bagus Made Oka; Ida Bagus Kade Suardana
Buletin Veteriner Udayana Bul. Vet. Udayana. December 2024 Vol. 16 No. 6
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i06.p03

Abstract

Colibacillosis is a disease caused by pathogenic Escherichia coli bacteria. Infected piglets experience weight loss, stunted growth and if not treated immediately will cause death. A 16-day-old Landrace piglet died with clinical signs of weakness and yellowish white diarrhoea for 4 days. The farmer's population was 25 pigs. Epidemiology showed morbidity of 8%, mortality of 4%, and case fatality rate of 50%. Anatomical pathology examination revealed distended intestines with bleeding and hyperaemic lungs. Bacterial culture of lung and intestinal organs showed positive results of gram-negative Escherichia coli bacteria. The results of parasitological examination of native, sedimentation and floating methods were negative for protozoan and helminth infections. Histopathology showed congestion, haemorrhage and neutrophil-dominated inflammatory cell infiltration in the intestines and lungs, while the brain, heart and liver were congested. Based on all laboratory examination results, the case pig was diagnosed with colibacillosis. Improved sanitation and biosecurity of the barn and barn environment needs to be routinely carried out.
THE THE EFFECT OF GREEN MENIRAN EXTRACT ON AVIAN INFLUENZA ANTIBODY TITER IN BROILERS TWO WEEKS BEFORE VACCINATION Dewandaru Evan Wintyassatya; Ida Bagus Kade Suardana; Romy Muhammad Dary Mufa; I Made Sukada; Ni Luh Eka Setiasih; I Made Kardena
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p32

Abstract

Avian Influenza (AI) is a viral disease that poses a serious threat to poultry farming, particularly in broiler production. The rapid and massive spread of AI in poultry flocks can cause substantial economic losses for farmers. Vaccination is one of the primary strategies for AI prevention; however, the currently available AI vaccines are killed/inactivated types, which tend to induce antibody titers slowly. This limitation, coupled with the relatively short rearing period of broilers, reduces vaccine effectiveness in broiler chickens. This study aimed to evaluate the effect of green meniran (Phyllanthus niruri Linn.) extract on enhancing antibody titers in broilers when administered for two weeks prior to AI vaccination. A total of 30 broiler chickens aged from day 1 to 32 days were used as experimental subjects. The study employed a Completely Randomized Design (CRD) with three groups: negative control (K−), positive control (K+), and treatment group (P). Data were analyzed using Analysis of Variance (ANOVA) and Regression Analysis via SPSS software. The results demonstrated a comparative increase in average antibody titers in the treatment group receiving Phyllanthus niruri extract compared to the control groups. It was concluded that supplementation with Phyllanthus niruri extract effectively enhanced antibody titers in broilers vaccinated with the inactivated AI La Sota vaccine, with peak antibody responses observed 1–2 weeks post-vaccination. The administration of green Phyllanthus niruri extract has been proven to stimulate antibody titers in broilers by accelerating the development of protective antibody levels against the AI virus. The timing of sample collection was shown to significantly affect antibody titers, with the highest levels observed during the first to second week post-vaccination. Future research on the administration of Phyllanthus niruri extract to improve antibody titers against Avian Influenza (AI) in broilers is expected to extend the duration of supplementation until the harvesting period and to include higher dosage variations in order to achieve more optimal and protective results.
CANINE PARVOVIRUS ENTERITIS INFECTION IN LOCAL DOGS IN SAMPLANGAN VILLAGE, GIANYAR REGENCY, BALI Fauzia Hadista Anjani; Ida Bagus Kade Suardana; I Gusti Ketut Suarjana; Ida Bagus Oka Winaya; Ida Ayu Pasti Apsari
Buletin Veteriner Udayana Bul. Vet. Udayana. October 2025 Vol. 17 No. 5
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i05.p01

Abstract

A common disease that poses a threat to dogs is Canine Parvovirus (CPV). Puppies tend to be more susceptible to CPV infection due to a period called the window of susceptibility. The purpose of this case study is to determine clinical changes that include anatomical pathology, histopathology, and laboratory examinations to establish a definitive diagnosis of the case animal. The animal used in the case study was a four-month-old female local puppy from Gianyar, Bali. The case dog was found freshly dead, and had been sick for 4 days. The dog showed symptoms of weakness, anorexia, and vomiting, bloody diarrhea. Polymerase Chain Reaction (PCR) test results were positive for Canine Parvovirus infection. Anatomical pathology changes found were congestion of the brain, hemorrhage of the trachea, swelling of the heart, hemorrhage of the liver, hemorrhage of the patu-peru, hyperemia of the renal medulla, blackening and swelling of the spleen, hemorrhage of the intestines, and bleeding of the stomach. Histopathological changes observed were tracheitis, necrotizing bronchopneumonia, myocarditis, hemorrhagic glomerulo-nephritis, speinitis, gastritis, and necrotizing enteritis. Based on clinical symptoms, anatomical and histopathological pathology observations, and Polymerase Chain Reaction (PCR) results, it can be concluded that the case dog was infected with Canine Parvovirus type enteritis. It is necessary to vaccinate dogs and pay attention to environmental cleanliness to minimize the transmission of Canine Parvovirus infection.
CASE OF STREPTOCOCCOSIS IN PIGLET AT TUA VILLAGE, MARGA, TABANAN, BALI Ni Kadek Puspa Dewi; I Gusti Ketut Suarjana; Ida Bagus Kade Suardana; Ida Bagus Oka Winaya; Ida Ayu Pasti Apsari
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p53

Abstract

Streptococcosis is a disease caused by streptococcal bacterial infection and can cause outbreaks in pig farms. This case study aims to discuss the pathological anatomy, histopathology, and laboratory test results to obtain a definitive diagnosis of the disease that attacks 7-day-old pigs from Tua Village, Marga, Tabanan. Data collection in the form of clinical signs, anamnesis, epidemiology, and laboratory tests to be further analyzed descriptively qualitatively. Clinical signs of pig cases are thin, weak, nervous symptoms (tremors, loss of balance, lying sideways, and cycling or rowing movements), white diarrhea, fatigue, and swelling of the leg joints. Epidemiological data show a morbidity of 8.13%, mortality of 3.25%, and a case fatality rate of 40%. Pathological anatomy examination was carried out through a necropsy procedure and found congestion of the brain, heart, and intestines, hemorrhage in the lungs, kidneys, stomach, and intestines, the spleen changed color to dark and swollen and the liver was swollen and uneven in color. Histopathological examination began with the preparation of histological preparations using Hematoxylin-Eosin (HE) staining, measurements were made by observing changes descriptively using a binocular light microscope with a magnification of 100x-1000x. The results of histopathological examination showed that the brain had meningoencephalitis hemorrhagica, lungs bronchopneumonia hemorrhagica, heart myocarditis, liver hepatitis, spleen splenitis hemorrhagica, kidney glomerulonephritis hemorrhagica et necrotican, stomach Gastritis hemorrhagica et necrotican, and intestine enteritis hemorrhagica et necrotican. Examination of histopathological preparations found inflammation dominated by neutrophil cells and macrophage cells. Bacteriological examination confirmed the presence of Streptococcus sp. bacterial infection in the brain, lungs, liver, and heart organs. Based on anamnesis, clinical signs, epidemiology, examination of anatomical and histopathological pathological changes, and bacteriological examination, it can be concluded that the pigs were infected with Streptococcosis due to Streptococcus suis bacteria. In pig farming management, optimal biosecurity and sanitation must be implemented to minimize the risk of contamination by disease agents.
THE IMMUNOMODULATORY EFFECT OF PHYLLANTHUS NIRURI L. EXTRACT ON ANTIBODY TITERS IN NEWCASTLE DISEASE-VACCINATED BROILERS Daniel Halim; Ida Bagus Kade Suardana; Tjokorda Sari Nindhia; Tri Komala Sari; Ni Luh Eka Setiasih; Anak Agung Sagung Kendran
Buletin Veteriner Udayana Bul. Vet. Udayana. December 2025 Vol. 17 No. 6
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i06.p06

Abstract

Newcastle Disease (ND) remains a major threat to the poultry industry, and while vaccination is essential, the immune response can be inconsistent. This study evaluated the potential of Phyllanthus niruri L. (meniran) extract as an immunomodulator to enhance the humoral immune response in broilers post-ND vaccination. A total of 30 one-day-old broilers, vaccinated against ND, were divided into three treatment groups: P0 (control, no extract), P1 (extract at 5 mg/kg BW/day via drinking water from day 3 to 17), and P2 (extract from day 3 to 24). Antibody titers were measured using the Hemagglutination Inhibition (HI) test and analysed with ANOVA using SPSS software. The results demonstrated that meniran extract significantly increased antibody titters compared to the control (P<0.05). Group P2, which received the extract until day 24, achieved the highest mean titter (6.1 log2 HI units), followed by P1 (4.4 log2) and the control P0 (4.0 log2). Data analysis confirmed a positive relationship between the duration of extract administration and antibody levels. In conclusion, Phyllanthus niruri L. extract is an effective immunomodulator that significantly enhances the ND vaccine-induced antibody response in broilers, with a longer administration period yielding a more robust effect.
Co-Authors Anak Agung Sagung Kendran Anak Agung Sagung Kendran Anak Agung Sagung Kendran Citra Widiawati Daniel Halim Dewandaru Evan Wintyassatya Dwi Arum Permatasari Fauzia Hadista Anjani Gusti Ayu Yuniati Kencana I Gusti Ketut Suarjana I Gusti Ketut Suarjana I Gusti Ketut Suarjana I Made Damriyasa I Made Indra Palaguna I Made Kardena I Made Sukada I Nyoman Sulabda Ida Ayu Pasti Apsari Ida Bagus Ketut Indra Permana Ida Bagus Made Oka Ida Bagus Oka Winaya Ni Kadek Puspa Dewi Ni Luh Eka Setiasih Ni Luh Eka Setiasih Ni Luh Eka Setiasih Ni Made Suksmadewi Wisnantari Romy Muhammad Dary Mufa Savira Hadi Putri Tjokorda Sari Nindhia Tjokorda Sari Nindhia Tjokorda Sari Nindhia Tri Komala Sari Yolla Noviolita