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All Journal Jurnal Kedokteran Brawijaya Jurnal Ilmiah Sultan Agung
Joko Wahyu Wibowo
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Religion Humanities Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Electrical & Electronics Engineering Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health

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Effects of Propolis Extract Supplementation during Pregnancy on Stress Oxidative and Pregnancy Outcome: Levels of Malondialdehyde, 8-Oxo-2′-Deoxogunosine, Maternal Body Weight, and Number of Fetuses Joko Wahyu Wibowo; Minidian Fasitasari; Siti Thomas Zulaikhah
Jurnal Kedokteran Brawijaya Vol. 31 No. 3 (2021)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2021.031.03.5

Abstract

Oxidative stress is related to pregnancy complications that could increase maternal and infant mortality. This study aimed to determine the effect of propolis extract supplementation during pregnancy on oxidative stress level and pregnancy outcomes utilizing Malonedealdehyde (MDA) and 8-Oxo-2′-Deoxogunosine (8-OHdG) levels, maternal body weight, and the average number of fetuses as the parameters. The study was conducted by using a posttest only control group design on 24 pregnant Wistar rats, which were divided into four groups. Group I was control, Group II-IV were the treatment groups given propolis extract of 1.8mg, 3.6mg, and 7.2mg/200gBW/day, respectively. The standard feed given was AIN93G dose of 20g/day and distilled water ad libitum. Propolis extract was given using a gastric feeding tube every morning for 20 days. At the end of the treatment, body weight was meisured and blood collected for assessed MDA and 8-OHdG levels  by ELISA method  and then we performed abdominal surgery to count number of fetuses. The result are there were decreasing level of MDA and 8-OHDG by administration of propolis significantly (p<0.05) group: I: 2,04±0,091, II: 1,55±0,067, III: 1,05±0,176, IV: 0,73±0,075 (mmol/mL) (p=0.001); 8 OHdG level (ng/mL) group I: 10,02±0,403, II: 8,60±0,078, III: 7,89±0,051, IV: 7,53±0,063 (p=0,001). Average of maternal body weight (g) were increased: group I: 228,33±3,93, II: 237,17±4,36, III: 244,83±4,02, IV: 248,00±5,76 (p=0,001) and Average number of fetuses tend to increased as well, group I : 8,5±0,05, II: 7,8±0,41, III: 9,5±1,05, IV: 9,6±0,52 (p=0,02). The conclusion of this research are supplementation of propolis extract in pregnant rats can reduce oxidative stress and improve pregnancy outcomes.
Pengaruh Ekstrak Biji Chia (Salvia Hispanica L) Terhadap Kadar Gula Darah Sewaktu dan Kadar MDA Syafinda Meisari Trisnani; Chodidjah .; Joko Wahyu Wibowo
Jurnal Ilmiah Penelitian Mahasiswa Vol 2, No 1 (2023): Maret 2023
Publisher : Jurnal Ilmiah Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.95 KB)

Abstract

Hiperglikemia merupakan kondisi dimana tubuh mengalami peningkatan kadar gula darah dan merupakan suatu manifestasi klinis dari DM tipe 2 yang akan meningkatkan ROS sehingga terjadi ketidakseimbangan antioksidan yang berakibat menjadi stres oksidatif salah satu biomarkernya yaitu MDA. Biji chia mengandung antioksidan yang tinggi sehingga efektif untuk menurunkan stres oksidatif yang diakibatkan oleh hiperglikemia pada DM tipe 2. Tujuan penelitian untuk membuktikan pengaruh ekstrak biji chia terhadap kadar GDS dan kadar MDA pada tikus model DMT2 dengan injeksi STZ. Metode penelitian ini adalah eksperimental dengan rancangan Posttest Only Control Group Design. Jumlah sampel 25 ekor tikus wistar jantan yang berusia 3-4 bulan dengan berat 180-200 gram dan diadaptasi selama 7 hari, dibagi 5 kelompok secara acak yaitu kelompok perlakuan P0 sebagai kontrol, P1 sebagai kontrol positif, P2 sebagai kontrol negatif, P3 untuk di injeksi STZ dan ekstrak biji chia dosis 4,5 mg/KgBB/hari, P4 untuk di injeksi STZ dan ekstrak biji chia dosis 13,5 mg/KgBB/hari. Lama perlakuan 7 hari. Sampel darah diambil dari v. Ophtalmica pada hari ke 14. Pemeriksaan kadar GDS menggunakan metode ELISA dan pemeriksaan kadar MDA menggunakan metode TBARS, data dianalisis dengan uji Anova. Hasil penelitian didapatkan rerata kadar GDS P0: 127,6±2,8; P1: 204,6±6,1; P2: 76,2±1,3; P3: 45,6±2,07; P4: 40±1,58. Rerata kadar MDA dalam satuan nmol/ml P0: 0,93±0,04; P1: 1,20±0,01; P2: 0,73±0,04; P3: 0,44±0,03; P4: 0,22±0,20. Hasil uji Anova diperoleh P value 0,000 dan hasil uji post hoc LSD antar kelompok diperoleh P value 0,000 (
Co-Authors Chodidjah . Minidian Fasitasari Siti Thomas Zulaikhah Syafinda Meisari Trisnani