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All Journal Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research
Zuraidawati Zuraidawati, Zuraidawati
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Use of Vinegar as A Candidate Substitute Of Acetic Acid (CH3COOH) in The Rivalta Test Method in Diagnosis Feline Infectious Peritonitis (FIP) Domestic Cat Zuraidawati, Zuraidawati; Dewi, Maryulia; Syah, Nanda Yulian; Zamzami, Rumi Sahara
The International Journal of Tropical Veterinary and Biomedical Research Vol 7, No 2 (2022): Vol. 7 (2) November 2022
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/ijtvbr.v7i2.30497

Abstract

This study aims to test the samples of abdominal fluid from cats suspected of having FIP by using vinegar as a substitute for acetic acid in the rival method. Feline Infectious Peritonitis (FIP) is a viral disease that is not zoonotic, but is very fatal with an infectious prognosis in various types of cats and its transmitted orally. This study aims to determine the positive or negative results of FIP infection in cats. The diagnosis of FIP can be seen through clinical symptoms such as lack of appetite, diarrhea, weakness, dehydration (reaching 8%), Body Condition Score (BCS) 2, enlarged abdomen, and ultrasound results showing ascites and swollen kidneys. This study uses vinegar as a substitute for acetic acid in the rival method. The test was carried out experimentally in a laboratory. The research data were analyzed descriptively, the results of a positive FIP reaction were indicated by the presence of a cloudy jellyfish-shaped precipitate in the test tube.
ANTIBACTERIAL ACTIVITY AND HEMATOLOGICAL PROFILE OF RAT (Rattus norvegicus) DUE TO ADMINISTRATION OF ETHANOL EXTRACT OF SOURSOP FLOWERS (Annona muricata L.) AND Salmonella enteritidis INFECTION Zuraidawati, Zuraidawati; Sugito, Sugito; Darmawi, Darmawi; Taufiq, Taufiq
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i2.11646

Abstract

This study aimed to determine the antibacterial activity of soursop flower extract (Annona muricata L.) and hematological profile of rats (Rattus norvegicus) due to administration of soursop flower ethanol extract and Salmonella enteritidis infection. The concentrations of soursop flower ethanol extract used for the antibacterial activity test were 25%, 50%, 75%, 100%, with the antibiotic ampicillin 10 g/disk was used s a positive control (PC) and dimethyl sulfoxide (DMSO) 10% as a negative control (NC). For examination of hematological features, 15 male rat aged two months were used. All rats were divided into 5 treatment groups, each consisting of three rats. The NC group was not given soursop flower ethanol extract and S. enteritidis infection. The PC group was not given soursop flower extract but was given S. enteritidis infection. Groups P1, P2, and P3 were given ethanol extracts of soursop flower at a dose of 0.18, 0.36, and 0.72 g/rat/day peroral for a week using gastric sonde. On the following day (after administration of soursop flower extract) the first blood drawing was performed. All rats, except NC group, were then infected with S. enteritidis intraperitonially at dose of 3x108 CFU/mL dose (0.5 mL McFarland). One week after being infected with S. enteritidis, a second blood drawing was performed. The results of the antibacterial activity test showed that there was no antibacterial activity was observed since no inhibition at various concentrations was formed. The administration of soursop flower extract at various dosage levels was able to maintain the number of leukocytes but reduced the number of erythrocytes, hemoglobin concentration, hematocrit value and the number of platelets in rats; whereas S. enteritidis infection decreased all the hematologic features of lab rats.
Co-Authors Adhayani, Layli Darmawi . Erina Erina Maryulia Dewi Nanda Yulian Syah Sugito - Taufiq Taufiq Zamzami, Rumi Sahara