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Aktivitas Antibiotik terhadap Biofilm Staphylococcus aureus Isolat Preputium Sapi Aceh Dewi, Maryulia; Darmawi, Darmawi; Nurliana, Nurliana; Karmil, T. Fadrial; Helmi, T. Zahrial; Fakhrurrazi, Fakhrurrazi; Erina, Erina; Abrar, Mahdi; AK, M. Daud; Admi, Masda
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (468.862 KB) | DOI: 10.22146/jsv.57359

Abstract

Biofilm is one of parameters for determining pathogenic pathways of a bacterium. Bacterial virulence activities can be attenuated by antibiotics with inhibiting biofilm. The aim of this study was to examine the characteristics and activities of antibiotics against the biofilm of Stapylococcus aureus isolate which taken from Aceh cattle prepuce. The study was exploratory and experimental, using a Completely Randomized Design (RAL) factorial pattern using three types of antibiotics, three concentrations and replications. Antibiotics were tetracycline, oxytetracycline and phosphomycin with concentrations of 10%, 20% and 30% (mg/mL), respectively. Therefore, the aim of this study was to examine the characteristics and activities of antibiotics to the biofilm of Stapylococcus aureus from Aceh cattle prepuce isolate. U-bottom polystyrene 96 wells microplate and Nutrient Broth (NB) medium was used to biofilm bacterial while biofilm was stained on crystals violet 1%. Optical Density (OD) of biofilm were λ 595 nm. Data were analyzed by using Analysis of Variance (ANOVA). The results showed that the activity of Staphylococcus aureus was moderately positive, i.e 2,667cell/μL. It showed that antibiotics and concetrations have significant effect (P<0.05) to inhibit biofilm staphylococcus aureus. In additioon, tetracycline 30% (mg / mL) has the best ability to inhibit biofilms from 52,18% to 1,262 cell/μL. Based on that fact, it can be concluded that the virulence of Staphylococcus aureus was moderate positive to be false positive.
Analisis Peningkatan Sikap Kolaborasi Siswa melalui Penerapan Profil Pelajar Pancasila pada Dimensi Gotong Royong di Sekolah Dasar Erina, Erina; Manan, Nanan Abdul
Jurnal Basicedu Vol. 8 No. 4 (2024)
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/basicedu.v8i4.8448

Abstract

Profil pelajar pancasila bertujuan melahirkan generasi emas yang memiliki jiwa atau sikap pancasila yang tertanam pada diri siswa sebagai bekal bagi kehidupan siswa selanjutnya. Oleh karena itu, penelitian itni berangkat dari permasalahan dimana beberapa siswa masih banyak yang belum mampu untuk dapat berkolaborasi dan melakukan kegiatan gotong royong, untuk itu penelitian ini bertujuan untuk mencari tahu dan menganalisis faktor apa saja yang dapat memperkuat sikap kolaborasi siswa dalam dimensi gotong royong profil pelajar pancasila. Peneliti menggunakan jenis penelitian kualitatif deskriptif dengan desain studi kasus, peneliti menggunakan instrumen triangulasi data berupa observasi, wawancara kepada 3 pihak yaitu kepala sekolah, guru wali kelas, dan yang paling utama yaitu siswa, dan dokumentasi. Hasil dari penelitian ini ditemukan bahwa faktor yang dapat mempengaruhi kuatnya sikap kolaborasi siswa bukan hanya disebabkan dari dalam diri siswa, namun disebabkan dari faktor luar seperti lingkungan keluarga dan masyarakat serta kebiasaan yang dibangun siswa dalam kegidupan sehari-hari. Oleh karena itu damapak dari penelitian ini salah satunya memberi masukan kepada pihak sekolah terutama guru wali kelas dalam memberikan pemahan dan penanaman sikap profil pelajar pancasila
Analisis Peningkatan Sikap Kolaborasi Siswa melalui Penerapan Profil Pelajar Pancasila pada Dimensi Gotong Royong di Sekolah Dasar Erina, Erina; Manan, Nanan Abdul
Jurnal Basicedu Vol. 8 No. 4 (2024)
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/basicedu.v8i4.8448

Abstract

Profil pelajar pancasila bertujuan melahirkan generasi emas yang memiliki jiwa atau sikap pancasila yang tertanam pada diri siswa sebagai bekal bagi kehidupan siswa selanjutnya. Oleh karena itu, penelitian itni berangkat dari permasalahan dimana beberapa siswa masih banyak yang belum mampu untuk dapat berkolaborasi dan melakukan kegiatan gotong royong, untuk itu penelitian ini bertujuan untuk mencari tahu dan menganalisis faktor apa saja yang dapat memperkuat sikap kolaborasi siswa dalam dimensi gotong royong profil pelajar pancasila. Peneliti menggunakan jenis penelitian kualitatif deskriptif dengan desain studi kasus, peneliti menggunakan instrumen triangulasi data berupa observasi, wawancara kepada 3 pihak yaitu kepala sekolah, guru wali kelas, dan yang paling utama yaitu siswa, dan dokumentasi. Hasil dari penelitian ini ditemukan bahwa faktor yang dapat mempengaruhi kuatnya sikap kolaborasi siswa bukan hanya disebabkan dari dalam diri siswa, namun disebabkan dari faktor luar seperti lingkungan keluarga dan masyarakat serta kebiasaan yang dibangun siswa dalam kegidupan sehari-hari. Oleh karena itu damapak dari penelitian ini salah satunya memberi masukan kepada pihak sekolah terutama guru wali kelas dalam memberikan pemahan dan penanaman sikap profil pelajar pancasila
Detection of Pathogenic Escherichia coli in Duck Intestine Slaughtered at Lambaro Market Aceh Besar Erina, Erina; Panjaitan, Tasya Adilla; AK, M. Daud; Sari, Wahyu Eka; Rusli, Rusli; Helmi, Teuku Zahrial
Jurnal Medika Veterinaria Vol 19, No 1 (2025): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v19i1.44883

Abstract

Pathogenic Escherichia coli is a type of bacteria that has the ability to cause disease in hosts, including humans and animals, although in general E. coli acts as a normal flora in the digestive tract. This study aimed to detect the presence of pathogenic E. coli in the intestines of ducks sold at Lambaro Market, Aceh Besar. E. coli is a normal flora bacteria in the digestive tract of animals and humans, but some strains are pathogenic and can cause zoonotic diseases. The presence of pathogenic E. coli in poultry products has the potential to be a source of infection for humans through consumption of contaminated food. This study used 15 duck intestine samples obtained from cecum, to identify the presence of pathogenic E.coli. The results showed that 13 samples produced clear colonies on Sorbitol MacConky Agar (SMAC), indicating the inability of bacteria to ferment sorbitol, which correlated to the presence of pathogenic E. coli. In contrast, two samples showed pink colonies indicating the presence of nonpathogenic E. coli. The conclusion of this study was the cecum of ducks sold at Lambaro Market, Aceh Besar positive for pathogenic E. coli.
DETECTION OF MULTIDRUG-RESISTANT Klebsiella pneumoniae IN THE UPPER RESPIRATORY TRACT OF BROILERS AND LAYER CHICKENS FROM COMMUNITY FARM IN ACEH BESAR, INDONESIA Darniati, Darniati; Erina, Erina; Abrar, Mahdi; Zainuddin, Zainuddin; AK, M Daud
Jurnal Kedokteran Hewan Vol 19, No 2 (2025): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v19i2.45844

Abstract

This study aimed to investigate the presence of multidrug-resistant (MDR) Klebsiella pneumoniae in upper respiratory tract of broiler chickensand laying hens from community farms in Aceh Besar. Tracheal swab samples were collected and cultured on eosin methylene blue agar for bacterialisolation. Suspected isolates were identified through biochemical testing and confirmed via PCR targeting the rpoB gene. Identified isolates weretested for antibiotic susceptibility against ciprofloxacin, tetracycline, gentamicin, erythromycin, and chloramphenicol, using the disc diffusionmethod. The results revealed K. pneumoniae in 6% of broiler and 14% of laying hens, with widespread multidrug resistance. All isolates wereresistant to erythromycin (100%), gentamicin (100%), tetracycline (85%), ciprofloxacin (40%), and chloramphenicol (15%). These findingsconcluded that there has been the spread of MDR of K. pneumoniae in broiler chickens and laying hens within Aceh Besars community farms inAceh Besar, Indonesia.
Isolation of Aspergillus sp. from The Lungs of Sparrows (Passer montanus) Nugraha, Ronaldi Fajar; Erina, Erina; AK, M. Daud; Jamin, Faisal; Darniati, Darniati
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.102705

Abstract

Aspergillosis is a common respiratory disease in avian species. The infection of Aspergillus sp. is characterized by the presence of yellowish plaques or nodules in the respiratory tract such as the trachea, air sac, and lungs. This study aimed to isolate Aspergillus sp. from the lungs of sparrows. The samples used in this research were the lungs of 35 sparrows taken randomly. Isolation was carried out by Thompson’s method (1969), samples were embedded in Saboraud’s Dextrose Agar (SDA) media and then incubated for 2-7 days. The growth of Aspergillus sp. colonies was observed at 24-hour intervals by looking at the color, shape, edges, and bottom surface of the colonies. Meanwhile, to ensure and confirm the species of Aspergillus sp., the fungus was planted on slide culture media. After that, microscopic observations were conducted by looking at the growth of conidiophores, hyphae, phialids, and fungal conidia. The data obtained were then analyzed descriptively. The result showed that Aspergillus sp. can be isolated from 27 out 35 samples. Based on the result of this study it can be concluded that 77.14% of samples were infected by Aspergillus sp. consisting of Aspergillus niger and Aspergillus flavus.
Edu Parenting sebagai Sarana Sosialisasi Pengetahuan dan Pemahaman Orang Tua Terhadap Gizi Seimbang Anak Usia Dini Erina, Erina; Jayanti, Diana Dwi; Fitriana, Dina; Jihansyah, Imas
JCE (Journal of Childhood Education) Vol 7 No 2 (2023): JCE (Journal of Childhood Education) September-February
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/jce.v7i2.1932

Abstract

This study entitled "Edu-parenting as a means of disseminating knowledge and understanding of parents on balanced nutrition for early childhood at SPS Roudlotul Jannah". This was motivated by the knowledge and understanding of parents regarding balanced nutrition for early childhood which has an important role for children because it will affect their growth and development. As an educational institution which was also a place for children to grow and develop, the school should also anticipate this by organizing an educational parenting activity as a means of disseminating knowledge and understanding of balanced nutrition for early childhood, so that parents can provide optimal and appropriate balanced nutrition consumption patterns for their children. Based on preliminary study conducted by researchers, it was known that the average parents at SPS Roudlotul Jannah, who was the participant in this research, still didn’t have optimal knowledge and understanding of balanced nutrition according to the needs of the child's body. This study used a descriptive qualitative approach and is a type of field research with 10 subjects. The results of this study indicated that the edu parenting activities carried out on April 4, 2023 can be used as a means of socializing parental knowledge and understanding of balanced nutrition for early childhood which can be seen from the inscreasing of : (1) new knowledge and understanding of balanced nutrition for early childhood, and (2) the commitment and awareness of parents regarding the provision of balanced nutrition for children after participating in edu-parenting activities.
Resistance Testing of Salmonella sp. Isolated from Broiler Chicken against Antibiotics Syafitri, Martiana; Erina, Erina; AK, M Daud; Ferasyi, Teuku Reza; Hamzah, Abdullah; Nazaruddin, Nazaruddin; Ismail, Ismail
Jurnal Medika Veterinaria Vol 17, No 1 (2023): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v17i1.20301

Abstract

Antibiotic resistance has become a global problem, this happens because strains of bacteria in the body are resistant to antibiotics, one of which is caused by improper use. This study aims to determine the resistance of Salmonella sp. isolated from broiler chickens to antibiotics. The method used for this study is the Kirby Bauer disc diffusion method. The research samples were eight isolates from broiler chickens collected by the Laboratory of Microbiology, Faculty of Veterinary Medicine, Universitas Syiah Kuala. This study used antibiotics amoxicillin, streptomycin, chloramphenicol, ciprofloxacin, gentamicin, and tetracycline. The data obtained are processed descriptively. The results showed five isolates (62.5%) showed multidrug resistance and very high levels of resistance to antibiotics from eight samples tested, namely tetracycline 87.5% (7/8), chloramphenicol 75% (6/8), amoxicillin 62.5% (5/8), and streptomycin 62.5% (5/8). Antibiotics that are still sensitive are gentamicin 62.5% (5/8) and ciprofloxacin 50% (4/8). Therefore, it can be concluded that Salmonella sp. has been resistant to several types of antibiotics.
ANTI-TRYPANOSOMA ACTIVITY OF ETHANOLIC EXTRACT OF NEEM LEAF (Azadirachta indica) ON Trypanosoma evansi IN RATS (Rattus norvegicus) Fahrimal, Yudha; Maghfirah, Siti; Rinidar, Rinidar; Azhar, Al; Asmilia, Nuzul; Erina, Erina
Jurnal Kedokteran Hewan Vol 11, No 1 (2017): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i1.5450

Abstract

The aim of this study was to determine the effect of neem leaf extract (Azadirachta indica) on parasitemia of rats infected with Trypanosoma evansi (T. evansi) Aceh local isolate. A total of 24 male rats aged three months were used in this study and randomly divided into six treatment groups equally. The negative control group (K0) without T. evansi infection and neem leaf extract, the positive control group (K1) was infected with T. evansi but no neem leaf extract given, group K2, K3, K4, and K5 were infected with 5x104 T. evansi and were given neem leaf extract after patent infection with dose of 50, 100, 400, and 800 mg/kg BW respectively. The extract was given orally for three consecutive days. On the fourth day, rat blood was drawn for parasitemia examination. The results showed that no T. evansi detected in rats in negative control group (K0), while parasitemia in group K1; K2; K3; K4; and K5 was 12,295 x106/mL; 10,495 x106/mL; 9,360 x106/mL; 5,080x106/mL; and 2,398x106/mL of blood, respectively. Percentage of inhibition of parasitemia in K2, K3, K4, and K5 reached 14.64, 23.78, 58.68, and 80.50%, respectively. Based on the result of the study, neem leaf extract of 800 mg/kg BW gave the highest reduction of parasitemia in rats infected with T. evansi.
PENANAMAN NILAI-NILAI KARAKTER MELALUI LAGU BERTEMAKAN ALAM DI SEKOLAH MINGGU BUDDHA BUMI SUCI MAITREYA PEKANBARU Erina, Erina; Jelita, Rida; Rozana, Sri Diana
JURNAL PENDIDIKAN BUDDHA DAN ISU SOSIAL KONTEMPORER (JPBISK) Vol. 6 No. 1 (2024): Juni
Publisher : LPPM STAB Bodhi Dharma

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56325/jpbisk.v6i1.116

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui penanaman nilai-nilai budi pekerti melalui lagu-lagu bertemakan alam dan untuk mengetahui makna lagu yang terkandung dalam lirik lagu-lagu bertemakan alam di Sekolah Minggu Buddha Bumi Suci Maitreya Pekanbaru. Penelitian ini merupakan penelitian kualitatif dengan metode studi deskriptif. Subjek penelitian ini meliputi kepala SMB, guru dan siswa SMB. Teknik pengumpulan data yang digunakan adalah observasi, wawancara dan dokumentasi dan teknik analisis data menggunakan reduksi data, penyajian data dan kesimpulan. Tujuan dari penelitian ini adalah untuk mengetahui penanaman nilai-nilai budi pekerti melalui lagu-lagu bertemakan alam dan untuk mengetahui makna lagu yang terkandung dalam syair lagu-lagu bertemakan alam di Sekolah Minggu Buddha Bumi Suci Maitreya Pekanbaru. Penelitian ini merupakan penelitian kualitatif dengan metode studi deskriptif. Subjek penelitian ini meliputi kepala SMB, guru dan siswa SMB. Teknik pengumpulan data yang digunakan adalah observasi, wawancara dan dokumentasi dan teknik analisis data menggunakan reduksi data, penyajian data dan kesimpulan.