Garuda - Garba Rujukan Digital

Dewi Nuraini Setyowati

University of Mataram

Author-ID : 5673052

Agriculture, Biological Sciences & Forestry

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THE EFFECT OF EM4 PROBIOTIC ADMINISTRATION FOR GROWTH AND SURVIVAL OF VANAME SHRIMP (Litopenaeus vannamei) Lalu Wahyu Wilisetyadi; Dewi Nuraini Setyowati; Fariq Azhar
Journal of Fish Health Vol. 2 No. 2 (2022): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Vannamei shrimp is a shrimp that has high economic value in Indonesia. The poor aquatic environment can reduce shrimp growth rates and stress and cause death, and efforts to improve water quality by giving Probiotics EM4. Probiotic EM4 contains beneficial bacteria that can improve shrimp's digestive system, decompose organic matter in the waters, control the growth of pathogenic bacteria, and improve water quality. The experimental parameters included total hemocyte count (THC), differential hemocyte count (DHC) consisting of hyaline cells, granule cells, and semi-granule cells, and phagocytic activity (AF). The study was carried out for 69 days using vanamei shrimp with PL 30. This study used four treatments and three replications, namely treatment P0 (without giving probiotic EM4), P1 (giving probiotic EM4 at a dose of 0.5 ml/L), P2 (giving probiotic EM4 at a dose of 1.5 ml/ L), P3 (giving probiotic EM4 dose of 2.5 ml/L). The study's results proved that the administration of probiotic EM4 on the maintenance medium had a significant effect (P<0.05) on THC, DHC, and AF. At THC, the highest value was at P3 with a value of 19.44 x 10 6 cells/ml, and the lowest was at P0 with a value of 11.01 x 10 6 cells/ml, then the DHC values such as hyaline cells ranged from 51.34 – 65.81%, granule cells ranged from 21.33 – 30.4%, semi-granular cells ranged from 12.84 – 18.24%. And then for the highest AF value at P3 with a value of 67.97% and the lowest P0 with a value of 49.34%

EFFECT OF BETEL LEAF EXTRACT (Piper betle L.) ON Aeromonas hydrophila DISEASES IN TILAPIA (Oreochromis niloticus) SEED Ahmad Zaeroni; Dewi Nuraini Setyowati; Fariq Azhar
Journal of Fish Health Vol. 2 No. 2 (2022): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Seed is essential in aquaculture activities, but tilapia hatcheries often suffer from disease attacks caused by Aeromonas hydrophila bacteria, causing losses for hatchery businesses. One of the natural ingredients for treating Aeromonas hydrophila is betel leaf extract. This study aims to analyze the effect of betel leaf extract (Piper betle L.) on Aeromonas disease hydrophila on fish fry tilapia (Piper betle L.). This study used a completely randomized design (CRD) with 5 treatments and 3 replications. The treatment used was a dose of betel leaf extract (P1) 0.4 ml, (P2) 0.6 ml, (P3) 0.8 ml, (P4) positive control, (P5) negative control. The parameters observed were survival rate, specific growth rate, feed conversion ratio, absolute length growth, erythrocytes, hemoglobin, hematocrit, leukocytes, differential leukocytes, total bacteria count, and water quality. The results of this study showed that before and after the challenge test, the best average values were P3 leukocytes (1.06 and 1.4 x 10 5 ), erythrocytes (0.90 and 0.93 x 10 5 ), hemoglobin (10.9 and 9.7), hematocrit (10.7 and 10.3), total bacteria (7 x 10 1 ). Then the worst value on P1 leukocytes (0.74 and 0.87 x 10 5), erythrocytes (0.86 and 0.75 x 10 5 ), hemoglobin (10.7 and 7.7), hematocrit (10.3 and 9.3), total bacteria (74 x 10 2 ). The study concluded that the best treatment was found in treatment 3 with a dose of 0.8 ml of betel leaf extract, which significantly increased the immune system in tilapia seeds.

THE EFFECT OF THE SOAKING PERIOD IN NATURAL FEED TATTS of Chaetoceros sp. ON THE GROWTH AND VIABILITY OF PEARL CLASS (Pinctada maxima) SEEDS Satria Fajry; Dewi Nuraini Setyowati; Alis Mukhlis
Journal of Fish Health Vol. 2 No. 2 (2022): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Pearl oysters are a marine aquaculture commodity with high economic value. However, there are still many obstacles in pearl oyster cultivation activities, such as the need for quality seeds. This study aims to determine the effect of immersing pearl seeds in natural feed tanks of Chaetocheros sp. with different immersion periods on the growth and survival rate of pearl oyster (Pinctada maxima) seeds. The method used in this study is an experimental method using a completely randomized design (CRD), with 5 treatments and 3 replications, namely A (control/without immersion), B (0.5 hours), C (1 hour), D (1, 5 Hours) and E (2 Hours). The results showed that soaking the seeds in natural feed tanks had a significant effect on the growth of the shell (dorsal-ventral) of the pearl oyster Pinctada maxima (p<0.05). The highest growth was achieved in the 2-hour immersion period with an absolute growth value of 1.67 mm, a relative growth of 68.49% from the initial size, and a daily specific growth rate of 3.31% per day. This method must be tested on larger pearl oyster seeds and using other natural feed species and various natural mixes (multi-species).

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