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All Journal Jurnal Biologi Tropis Journal of Aquaculture and Fish Health Journal of Fish Health

Fariq Azhar

University of Mataram

Author-ID : 3821401

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Articles

The Addition of Red Betel Leaf Extract (Piper crocatum) in the Feed of Vannamei Shrimps (Litopenaeus vannamei) for Vibriosis Prevention Fariq Azhar; Muhammad Junaidi; Bagus Dwi Hari Setyono; Andre Rachmat
Journal of Aquaculture and Fish Health Vol. 10 No. 3 (2021): JAFH Vol. 10 No. 3 September 2021
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v10i3.25973

Vibriosis is a type of shrimp disease caused by Vibrio sp. In the disease control of consumption biota, it is highly recommended to use natural ingredients that are not carcinogenic, one of which is red betel leaves (Piper crocatum). This study aims to determine the best dose of red betel leaf extract (P. crocatum) mixed in Vannamei shrimp feed to prevent vibriosis. In this study, shrimps were reared for 40 days at a density of 20 fish/container. The treatments included positive control, P1 (without extract + bacterial infection), negative control P2 (without extract and without bacterial infection), P3 (0.5% extract + bacterial infection), P4 (1% extract + infection), and P5 (2% extract + bacterial infection). The application of red betel leaf extract at a dose of 0.5% resulted in 75% survival after infection with V. parahaemolyticus, Vannamei shrimps of THC 7.70×106 cells/mL, and DHC (hyaline 82.94% granular 20.10%). The number of bacteria and the number of vibrio in the intestine were 52×108 CFU/mL and 12×108 CFU/mL accordingly. The best dose was obtained at P3 (Feed +0.5% red betel leaf extract), seen from the increase in survival rate, the number of hemocytes, differential haemocyte counts, and a decrease in total bacteria, so the application of red betel leaf extract can be used in the cultivation of Vannamei shrimps as an immunostimulant.

Difference Long Irradiation on The Growth Rate of Kappaphycus Alvarezii Lulu Lutfiati; Nunik Cokrowati; Fariq Azhar
Jurnal Biologi Tropis Vol. 22 No. 1 (2022): January - March
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i1.3292

The study aimed to find out the long-standing difference in irradiation to the growth rate of Kappaphycus alvarezii in controlled containers. The method used is experimental. This study used a Complete Randomized Design (RAL) using four (4) treatments with 3 repeats, namely the treatment of P1 control (without irradiation), P2 (8 hours of irradiation), P3 (10-hour irradiation), P4 (12-hour irradiation). The data were analyzed using analysis of variance (ANOVA) at a rate of 5% with a confidence interval of 95% and continued with the Duncan test. In this study, the average results of absolute weight and the highest specific daily growth rate at P4 treatment of 25.67ÃÂ±1.53 gr, 3.30ÃÂ±0.06%, followed by P3 treatment 21ÃÂ±2 gr, 3.11ÃÂ±0.09%, P2 treatment 16ÃÂ±1 gr, 2.87ÃÂ±0.06%, and lowest P1 treatment 12.33ÃÂ±2.08 gr, 2.63ÃÂ±0.15%. The average value of light intensity in each treatment is P1 1053ÃÂ±41.00 lux, P2 1256ÃÂ±27.71 lux, P3 1479ÃÂ±0.58 lux, and P4 1668ÃÂ±34.64 lux. Water quality parameters during maintenance are in the normal limit range for seaweed maintenance. D treatment produces the highest growth value this is because the light is an energy source in the process of photosynthesis, in chlorophyll plants, photosynthesis is the main process determining the rate of growth. The quality and amount of light that enters affects the growth of seaweed. This research concluded that the maintenance of Kappaphycus alvarezii in controlled containers with different irradiation had a significant effect on growth. 12 hours irradiation (P4) gave the best growth with an absolute weight of 16.00 g, and a specific growth rate of 4.06% per day and gave the highest chlorophyll-a value of 2.72 mg/l. Meanwhile, the highest phycoerythrin value was found in (P1) without irradiation, which was 4.58 mg/l.

EFFECT OF ADDITIONAL LEAF EXTRACT OF CIPLUKAN (Physalis angulata L) ON FEED ON THE GROWTH PERFORMANCE OF TILAPIA (Oreochromis niloticus) Suaebatul Aslamiah; Fariq Azhar; Muhammad Marzuki
Journal of Fish Health Vol. 1 No. 2 (2021): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

The purpose of this study is to determine the effect of the effectiveness of the addition of ciplukan leaf extract (Physalis angulata L.) on feed to the growth performance of tilapia (Oreochromis niloticus). This research method uses a Complete RandomIzed Design (RAL). This study consisted of 4 treatments and 3 replays, so 12 experimental units were used. Dose treatment on P0 (feed without the addition of ciplukan leaf extract), P1 (Feed added ciplukan leaf extract with a dose of 4%), P2 (Feed added ciplukan leaf extract with a dose of 8%), and P3 (Feed added ciplukan leaf extract with a dose of 12%). This dosage treatment is based on Effendi &Hardi research (2014) with dose treatment of 4%, 8%, and 12% per 1kg of feed. the addition of ciplukan leaf extract with a concentration of 8% in feed provides absolute weight growth of (37.39±20.33), survival rate of (83.33±0.9 8), daily growth rate (1.25±0.60), and feed utilization efficiency of (4.67±0.58) and tend to be higher than other treatments.

THE EFFECT OF EM4 PROBIOTIC ADMINISTRATION FOR GROWTH AND SURVIVAL OF VANAME SHRIMP (Litopenaeus vannamei) Lalu Wahyu Wilisetyadi; Dewi Nuraini Setyowati; Fariq Azhar
Journal of Fish Health Vol. 2 No. 2 (2022): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Vannamei shrimp is a shrimp that has high economic value in Indonesia. The poor aquatic environment can reduce shrimp growth rates and stress and cause death, and efforts to improve water quality by giving Probiotics EM4. Probiotic EM4 contains beneficial bacteria that can improve shrimp's digestive system, decompose organic matter in the waters, control the growth of pathogenic bacteria, and improve water quality. The experimental parameters included total hemocyte count (THC), differential hemocyte count (DHC) consisting of hyaline cells, granule cells, and semi-granule cells, and phagocytic activity (AF). The study was carried out for 69 days using vanamei shrimp with PL 30. This study used four treatments and three replications, namely treatment P0 (without giving probiotic EM4), P1 (giving probiotic EM4 at a dose of 0.5 ml/L), P2 (giving probiotic EM4 at a dose of 1.5 ml/ L), P3 (giving probiotic EM4 dose of 2.5 ml/L). The study's results proved that the administration of probiotic EM4 on the maintenance medium had a significant effect (P<0.05) on THC, DHC, and AF. At THC, the highest value was at P3 with a value of 19.44 x 10 6 cells/ml, and the lowest was at P0 with a value of 11.01 x 10 6 cells/ml, then the DHC values such as hyaline cells ranged from 51.34 – 65.81%, granule cells ranged from 21.33 – 30.4%, semi-granular cells ranged from 12.84 – 18.24%. And then for the highest AF value at P3 with a value of 67.97% and the lowest P0 with a value of 49.34%

THE EFFECT OF ADDITION OF TURMERIC FLOUR (Curcuma longa Linn) INFEED ON THE PERFORMANCE OF THE GROWTH AND IMMUNE SYSTEM OF FISH (Oreochromis niloticus) Sari Damayanti; Sadikin Amir; Fariq Azhar
Journal of Fish Health Vol. 2 No. 2 (2022): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

This study aims to determine tilapia's growth and immune system (Oreochromis niloticus) through feeding with added turmeric flour. This study used a completely randomized design with five treatments, namely K- (feed without adding turmeric flour and injected with physiological solution), K+ (feed without adding turmeric flour and injected with A. hydrophila bacteria), P1 (addition of turmeric flour 1 % / kg of feed + bacterial infection of A. hydrophilla), P2 (Addition of 2% turmeric flour/kg of feed + bacterial infection of A. hydrophilla), and P3 (Addition of 3% turmeric flour/kg of feed + bacterial infection of A. hydrophilla) each treatment had three replicates for a total of 15 trials. The results showed that there was a significant effect (p > 0.05) on growth but no significant effect (p < 0.05) on the survival of tilapia, so further tests were carried out to determine the best treatment in producing tilapia growth, it is known that P2 (Addition of 2% turmeric flour/kg of feed + bacterial infection of A. hydrophilla) is the best treatment in this study.

EFFECT OF BETEL LEAF EXTRACT (Piper betle L.) ON Aeromonas hydrophila DISEASES IN TILAPIA (Oreochromis niloticus) SEED Ahmad Zaeroni; Dewi Nuraini Setyowati; Fariq Azhar
Journal of Fish Health Vol. 2 No. 2 (2022): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Seed is essential in aquaculture activities, but tilapia hatcheries often suffer from disease attacks caused by Aeromonas hydrophila bacteria, causing losses for hatchery businesses. One of the natural ingredients for treating Aeromonas hydrophila is betel leaf extract. This study aims to analyze the effect of betel leaf extract (Piper betle L.) on Aeromonas disease hydrophila on fish fry tilapia (Piper betle L.). This study used a completely randomized design (CRD) with 5 treatments and 3 replications. The treatment used was a dose of betel leaf extract (P1) 0.4 ml, (P2) 0.6 ml, (P3) 0.8 ml, (P4) positive control, (P5) negative control. The parameters observed were survival rate, specific growth rate, feed conversion ratio, absolute length growth, erythrocytes, hemoglobin, hematocrit, leukocytes, differential leukocytes, total bacteria count, and water quality. The results of this study showed that before and after the challenge test, the best average values were P3 leukocytes (1.06 and 1.4 x 10 5 ), erythrocytes (0.90 and 0.93 x 10 5 ), hemoglobin (10.9 and 9.7), hematocrit (10.7 and 10.3), total bacteria (7 x 10 1 ). Then the worst value on P1 leukocytes (0.74 and 0.87 x 10 5), erythrocytes (0.86 and 0.75 x 10 5 ), hemoglobin (10.7 and 7.7), hematocrit (10.3 and 9.3), total bacteria (74 x 10 2 ). The study concluded that the best treatment was found in treatment 3 with a dose of 0.8 ml of betel leaf extract, which significantly increased the immune system in tilapia seeds.

ANTIBACTERIAL EFFECTIVENESS OF BLACK QUICK (Nigella sativa Linn) EXTRACT ON BACTERIA INHIBITORY Aeromonas hydrophila, AND MORPHOLOGY FISH INDIGO (Oreochromis niloticus) POST INFECTION Dwi Elfa Maharani; Salnida Yuniarti Lumbessy; Fariq Azhar
Journal of Fish Health Vol. 2 No. 2 (2022): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

The main problem in cultivation is a disease that causes less optimal growth and even death. This study aims to analyze the effectiveness of black cumin extract on the inhibition of A. hydrophila bacteria, analyze its active ingredients, analyze its toxicity level to Tilapia, and analyze the effect of the extract on the survival rate and morphology of Tilapia after being infected with A. hydrophila bacteria. The method used was a completely randomized design (CRD) which consisted of 3 stages. Inhibition zone with 5 treatments 2 repetitions, namely P0 (aquades), P1 (25%), P2 (50%), P3 (75%), and P4 (100%). LC50 with 4 treatments 2 replications using regression analysis with the help of the probit table. In Vivo with 6 treatments, 3 replicates, namely P0 (NaCl injection) as a control-, P1 (bacterial injection) as control+, P2 (250 ppm), P3 (500 ppm), P4 (750 ppm), P5 (1000 ppm). The results showed that black cumin seed extract with various concentrations affected the inhibition of A. hydrophila, SR, and Tilapia morphology after infection with bacteria. The test of inhibition with concentrations of 25%, 50%, 75%, and 100% can inhibit the growth of bacteria A. hydrophila because it contains antibacterial activity compounds. The extract has toxic properties to Tilapia (LC50=220.23 ppm). Soaking fish for 30 minutes in the I n Vivo test P3 treatment is the best treatment because it can provide a high SR of 73.3 %. However, at high concentrations, it can reduce fish survival.

Co-Authors Ahmad Zaeroni Andre Rachmat Bagus Dwi Hari Setyono Dewi Nuraini Setyowati Dwi Elfa Maharani Lalu Wahyu Wilisetyadi Lulu Lutfiati Lumbessy, Salnida Yuniarti Muhammad Junaidi Muhammad Marzuki Nunik Cokrowati Sadikin Amir Sari Damayanti Suaebatul Aslamiah

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