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All Journal Wahana : Tridarma Perguruan Tinggi
Mardiyanto Riski Hartono
Universitas Hang Tuah
Agriculture, Biological Sciences & Forestry Humanities Decision Sciences, Operations Research & Management Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Languange, Linguistic, Communication & Media Nursing

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Potensi Ekstrak Air Dan Ekstrak Etanol Daun Avicennia Marina Sebagai Nutraceutical Mardiyanto Riski Hartono
WAHANA Vol 73 No 1 (2021): Wahana
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/wahana.v73i1.3595

Abstract

This research was conducted to prove that the leaves of avicennia marina contain ingredients that are potential as nutraceuticals, as well as to determine the comparison of the extraction results using water extract and ethanol extract. Avicennia marina leaves collected randomly and turn it into powder. The avicennia marina leaves powder processed with water extraction and ethanol extraction. The result of extraction process were tested by phytochemical screening tests to determine the content of flavonoids, alkaloids, tannins, steroids, triterpenoids, saponins, and examined for flavonoid levels by thin layer chromatography (TLC). The results of phytochemical screening test for ethanol extract of avicennia marina leaves showed positive results for the content of flavonoids, alkaloids, tannins, steroids, and saponins, but showed negative results for triterpenoid content. The water extract of avicennia marina leaves showed positive results for the content of flavonoids, alkaloids, tannins, and steroids, but showed negative results for saponins and triterpenoids content. At thin layer chromatography test, the flavonoid content showed that ethanol extract of avicennia marina leaves had 2.46%, while water extract of avicennia marina leaves had .80% of flavonoid content percentage. The results of ethanol extraction and water extraction from the leaves of avicennia marina show that they contain phytochemicals which are beneficial for health, and have the potential as a nutraceutical for medicinal purposes.
BIOKOMPATIBILITAS EKSTRAK DAUN MANGROVE DAN KOMBINASI DENGAN ASCORBIC ACID PADA SEL FIBROBLAS: Indonesia Mardiyanto Riski Hartono
WAHANA Vol 73 No 2 (2021): Wahana
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/wahana.v73i2.4635

Abstract

Latar belakang Penyembuhan luka dapat mengalami gangguan sehingga perlu pengobatan. Sel fibroblas adalah salah satu faktor penting pada proses penyembuhan luka. Penggunakan growth factor efektif meningkatkan fibroblas tetapi memiliki efek samping, dan sulit diperoleh. Ekstrak daun mangrove avicennia marina dan kombinasinya dengan ascorbic acid memiliki potensi meningkatkan jumlah sel fibroblas. Tujuan Untuk mengetahui biokompatibilitas dan dampak ekstrak daun mangrove avicennia marina dan kombinasinya dengan ascorbic acid terhadap proliferasi sel fibroblas. Metode Uji MTT Assay pada kultur sel yang diberi perlakuan dan dibagi menjadi 7 kelompok yaitu kelompok Ascorbic Acid 200μg/mL (P1), Ekstrak Etanol Daun Mangrove 100μg/mL (P2), Ekstrak Etanol Daun Mangrove 200μg/mL (P3), Ekstrak Air Daun Mangrove 100μg/mL (P4), Ekstrak Air Daun Mangrove 200μg/mL (P5), Kombinasi Ekstrak Etanol Daun Mangrove 100μg/mL dan Ascorbic Acid 200μg/mL (P6), Kombinasi Ekstrak Air Daun Mangrove 100μg/mL dan Ascorbic Acid 200μg/mL (P7). Kultur sel diperiksa dengan mikroskop, dan dibaca nilai optical density dengan Elisa Reader dan dihitung persentase sel hidup. Hasil Data nilai optical density dianalisa statistik. Seluruh kelompok perlakuan (P1-P7) bersifat biokompatibel (sel hidup ≥ 60%). Semua kelompok perlakuan memiliki persentase sel hidup diatas kontrol sel, dan kelompok P1, P3, P4, P5, P6, dan P7 memiliki perbedaan signifikan (p≤0,05), tetapi kelompok P2 tidak memiliki perbedaan signifikan (p>0,05). Kesimpulan Terapi ekstrak daun mangrove avicennia marina dan kombinasinya dengan ascorbic acid bersifat biokompatibel dan mampu menstimuli proliferasi sel fibroblas.
Potensi Ekstrak Air Dan Ekstrak Etanol Daun Avicennia Marina Sebagai Nutraceutical Mardiyanto Riski Hartono
WAHANA Vol 73 No 1 (2021): Wahana
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/wahana.v73i1.3595

Abstract

This research was conducted to prove that the leaves of avicennia marina contain ingredients that are potential as nutraceuticals, as well as to determine the comparison of the extraction results using water extract and ethanol extract. Avicennia marina leaves collected randomly and turn it into powder. The avicennia marina leaves powder processed with water extraction and ethanol extraction. The result of extraction process were tested by phytochemical screening tests to determine the content of flavonoids, alkaloids, tannins, steroids, triterpenoids, saponins, and examined for flavonoid levels by thin layer chromatography (TLC). The results of phytochemical screening test for ethanol extract of avicennia marina leaves showed positive results for the content of flavonoids, alkaloids, tannins, steroids, and saponins, but showed negative results for triterpenoid content. The water extract of avicennia marina leaves showed positive results for the content of flavonoids, alkaloids, tannins, and steroids, but showed negative results for saponins and triterpenoids content. At thin layer chromatography test, the flavonoid content showed that ethanol extract of avicennia marina leaves had 2.46%, while water extract of avicennia marina leaves had .80% of flavonoid content percentage. The results of ethanol extraction and water extraction from the leaves of avicennia marina show that they contain phytochemicals which are beneficial for health, and have the potential as a nutraceutical for medicinal purposes.
Biokompatibilitas Ekstrak Daun Mangrove Dan Kombinasi Dengan Ascorbic Acid Pada Sel Fibroblas: Indonesia Mardiyanto Riski Hartono
WAHANA Vol 73 No 2 (2021): Wahana
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/wahana.v73i2.4635

Abstract

Latar belakang Penyembuhan luka dapat mengalami gangguan sehingga perlu pengobatan. Sel fibroblas adalah salah satu faktor penting pada proses penyembuhan luka. Penggunakan growth factor efektif meningkatkan fibroblas tetapi memiliki efek samping, dan sulit diperoleh. Ekstrak daun mangrove avicennia marina dan kombinasinya dengan ascorbic acid memiliki potensi meningkatkan jumlah sel fibroblas. Tujuan Untuk mengetahui biokompatibilitas dan dampak ekstrak daun mangrove avicennia marina dan kombinasinya dengan ascorbic acid terhadap proliferasi sel fibroblas. Metode Uji MTT Assay pada kultur sel yang diberi perlakuan dan dibagi menjadi 7 kelompok yaitu kelompok Ascorbic Acid 200μg/mL (P1), Ekstrak Etanol Daun Mangrove 100μg/mL (P2), Ekstrak Etanol Daun Mangrove 200μg/mL (P3), Ekstrak Air Daun Mangrove 100μg/mL (P4), Ekstrak Air Daun Mangrove 200μg/mL (P5), Kombinasi Ekstrak Etanol Daun Mangrove 100μg/mL dan Ascorbic Acid 200μg/mL (P6), Kombinasi Ekstrak Air Daun Mangrove 100μg/mL dan Ascorbic Acid 200μg/mL (P7). Kultur sel diperiksa dengan mikroskop, dan dibaca nilai optical density dengan Elisa Reader dan dihitung persentase sel hidup. Hasil Data nilai optical density dianalisa statistik. Seluruh kelompok perlakuan (P1-P7) bersifat biokompatibel (sel hidup ≥ 60%). Semua kelompok perlakuan memiliki persentase sel hidup diatas kontrol sel, dan kelompok P1, P3, P4, P5, P6, dan P7 memiliki perbedaan signifikan (p≤0,05), tetapi kelompok P2 tidak memiliki perbedaan signifikan (p>0,05). Kesimpulan Terapi ekstrak daun mangrove avicennia marina dan kombinasinya dengan ascorbic acid bersifat biokompatibel dan mampu menstimuli proliferasi sel fibroblas.
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