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All Journal Wood Research Journal : Journal of Indonesian Wood Research Society Wood Research Journal : Journal of Indonesian Wood Research Society Jurnal Ilmu Kehutanan
Ganis Lukmandaru
Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry

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Total Phenolic, Flavonoid, Tannin Content and DPPH Scavenging Activity of Caesalpinia sappan Linn. Bark Brandon Aristo Verick Purba; Rini Pujiarti; Masendra Masendra; Ganis Lukmandaru
Wood Research Journal Vol 13, No 2 (2022): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2022.13.2.63-68

Abstract

Caesalpinia sappan is a shrubby Leguminosae tree commonly found in Indonesia, traditionally utilized as natural dye and herbal drink. However, in the making of traditional herbal drink, the bark is often discarded as residues. This research aimed to investigate total phenolic (TPC), flavonoid (TFC), and tannin (TTC) content as well as the antioxidant activity (DPPH scavenging activity) of the bark successive extracted with n-hexane, ethyl acetate, methanol, and hot water as well as analyzed it with GC-MS. The result showed the highest amount of TPC (824.16±62.28 mg GAE/g), TFC (185.03±1.91 mg QE/g), and TTC (987.07±30.98 mg TAE/g) in the methanol extract of the bark. GC-MS analysis resulted hydroquinone as a major constituent in the methanol extract. Further, antioxidant activity was found the strongest on methanol extract (IC50=63.48), while correlation between antioxidant activity and TFC was found the highest (R2=0.93). These findings suggest that bark of C. sappan is a suitable source of natural antioxidant with strong activity to DPPH radical.
Total Phenolic, Flavonoid, Tannin Content and DPPH Scavenging Activity of Caesalpinia sappan Linn. Bark Brandon Aristo Verick Purba; Rini Pujiarti; Masendra Masendra; Ganis Lukmandaru
Wood Research Journal Vol 13, No 2 (2022): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2022.13.2.63-68

Abstract

Caesalpinia sappan is a shrubby Leguminosae tree commonly found in Indonesia, traditionally utilized as natural dye and herbal drink. However, in the making of traditional herbal drink, the bark is often discarded as residues. This research aimed to investigate total phenolic (TPC), flavonoid (TFC), and tannin (TTC) content as well as the antioxidant activity (DPPH scavenging activity) of the bark successive extracted with n-hexane, ethyl acetate, methanol, and hot water as well as analyzed it with GC-MS. The result showed the highest amount of TPC (824.16±62.28 mg GAE/g), TFC (185.03±1.91 mg QE/g), and TTC (987.07±30.98 mg TAE/g) in the methanol extract of the bark. GC-MS analysis resulted hydroquinone as a major constituent in the methanol extract. Further, antioxidant activity was found the strongest on methanol extract (IC50=63.48), while correlation between antioxidant activity and TFC was found the highest (R2=0.93). These findings suggest that bark of C. sappan is a suitable source of natural antioxidant with strong activity to DPPH radical.
KADAR EKSTRAKTIF BATANG POHON JABON PUTIH (Neolamarckia cadamba) DAN JABON MERAH (Neolamarckia macrophyllus) DARI WONOGIRI, JAWA TENGAH Fatra Valahatul Ihda; Ganis Lukmandaru; Arif Nirsatmanto
Jurnal Ilmu Kehutanan Vol 17 No 1 (2023): March
Publisher : Faculty of Forestry Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jik.v17i1.6645

Abstract

Jabon is a fast-growing tree species with two types, white and red. The families or the origin of the mother tree are sources of variation that influence the chemical composition, particularly the extractive properties. This research aimed to determine the chemical properties of the wood and bark of 10-year-old white Jabon (families 23, 11, and 6) and 5-year-old red Jabon (families 75, 85, and 2) planted in a progeny test plot in Wonogiri, Central Java. In the wood portion of white Jabon, family 23 had the highest n-hexane (HEC), methanol (MEC), and hot water (HWEC) soluble extractive contents, as well as total extractive content (TEC) and total flavonoid content (TFC), while family 6 had the highest total phenolic content (TPC). Meanwhile, in red Jabon, family 75 had the highest HEC, HWEC, and TFC, family 2 had the highest MEC and TEC, and family 85 had the highest TPC. In the bark portion, family 11 had the highest HEC, MEC, TEC, and TPC, while family 6 had the highest HWEC and TFC for white Jabon. In red Jabon, family 2 had the highest HEC, family 85 had the highest MEC, HWEC, and TEC, while family 75 had the highest TPC and TFC.
Evaluasi Aktivitas Antijamur dan Antioksidan dari Ekstrak Etil Asetat Kulit Kayu Pinus merkusii Masendra Masendra; Brandon Aristo Verick Purba; Ganis Lukmandaru
Jurnal Ilmu Kehutanan Vol 15 No 1 (2021): Maret
Publisher : Faculty of Forestry Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3144.701 KB) | DOI: 10.22146/jik.v15i1.1494

Abstract

Pinus merkusii is the only species in the Pinaceae family to grow in Southeast Asia, including Indonesia, and serves as a source of wood and oleoresins. During the utilization of this pine, the bark is discarded as residue, however, this residue is possibly a potential source of antifungal and antioxidant agents, due to the polyphenol contents. This study, therefore, aims to investigate the antifungal and antioxidant compounds present in the bark of P. merkusii. The ethyl acetate extracts of Pinus merkusii were fractionated to obtain five fractions (Fr. A- Fr. E) and the bioactivity of each fraction was observed. Subsequently, the growth inhibition against Phanerochaete chrysosporium, was evaluated for antifungal activity, while DPPH (1,1-diphenyl-2-picrylhydrazyl) method was used to ascertain antioxidant activity. According to the GC-MS analysis, the ethyl acetate fractions contain triterpenoids and steroids as the dominant compounds as well as minor amounts of mono-sesquiterpenoids and hydrocarbons. The highest growth inhibition exhibited by Fr. C- Fr. E (>60%) indicates triterpenoids and steroids act as a fungal inhibitors. Meanwhile, the five fractions exhibited low antioxidant activity because the steroids and triterpenoids present are possibly ineffective DPPH inhibitors. Also, the antifungal and antioxidant activity R2 values were discovered to correlate 0.3, indicating a low positive correlation.
Co-Authors Arif Nirsatmanto Brandon Aristo Verick Purba Brandon Aristo Verick Purba Fatra Valahatul Ihda Masendra Masendra Rini Pujiarti