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Antifungal Activity of Triterpenoids and Steroids Isolated from Pinus merkusii Bark Against Phanerochaete chrysosporium Masendra Masendra; Brandon Arsito Verick Purba; Ganis Lukmandaru
Wood Research Journal Vol 11, No 2 (2020): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2020.11.2.65-71

The outer part of a tree, known as the bark, protects the internal tissues from external conditions and attacks from microorganisms. Its antifungal activities are due to the presence of chemicals in this bark. This study aimed at evaluating the toxicity of triterpenoids and steroids from the bark of Pinus merkusii against Phanerochaete chrysosporium (white-rot). The triterpenoids and steroids were isolated from n-hexane extract of the bark through column chromatography. Then, the antifungal activity was evaluated by measuring the growth rate of the fungus on potato dextrose agar (PDA) medium in a Petri dish. The isolation resulted in three triterpenoids (3β- methoxyserratt-14-en-21-one, serrate-14-en-3β,21β –diol, 3α,21β- dimethoxy-D14-serratene) and two steroids (β-sitosterol, Stigmast-4-en-3-one). There was more of antifungal activity with β-sitosterol and serrate-14-en-3β,21β –diol, due to presence of hydroxyl bond and their hydrophobicity.

Antifungal and Antioxidant Activities of Lipophilic Compounds from Swietenia mahagoni (l.) Jacq. Leaves Masendra Masendra; Brandon Aristo Verick Purba; Rizki Arisandi; Ganis Lukmandaru
Wood Research Journal Vol 6, No 2 (2015): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2015.6.2.62-68

The investigation on the n-hexane lipophilic extractives of Swietenia mahagoni, alongside the antifungal and antioxidant properties was conducted. The leaf sample was collected from 2- and 3-years-old trees in Perhutani enterprise of Temanggung, Central Java, Indonesia. In addition, the antifungal activity was tested using the white-rot of Phanerochaete chrysosporium, while the antioxidant property involved the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The results show antifungal of 28.6% and 37.5% growth inhibition in 2- and 3 -years-old leaf, respectively, while 21.7% and 25.4% antioxidant activity were also recorded. Furthermore, the GC-MS results showed the domination of S. mahagoni leaf by fatty acids and hydo-carbons, while the terpenoids and steroids were in minor quantities. These components possibly exhibit growth inhibitory and antioxidant effects against P. chrysosporium and DPPH radicals, respectively.

Extractives Contributing to the Color of Swietenia macrophylla King Bark Masendra Masendra; Rizki Arisandi; Brandon Aristo Verick Purba; Fuad Sumantri; Fatra Valahatul Ihda; Fatimah Zulaikha Wati; Ganis Lukmandaru
Wood Research Journal Vol 11, No 1 (2020): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2020.11.1.20-26

The bark of Swietenia machrophylla is colored dark red, and it is correlated with the extractives constituents such as phenolic compounds. This study, therefore, aims to investigate extractives from the inner and outer bark of S. machrophylla and their effects to color properties. The results showed that the extractive content in the inner bark was higher than the outer except for hot water solubles. In addition, the polyphenols and sugar levels from inner to outer bark were increased, except in the soluble-sugar of hot water extractive. The highest correlation between the absorbance of methanol, hot water-soluble extracts, and total polyphenols were observed using the visual spectrophotometer. The extractives that contributed to the bark's color were indicated from flavonoids with a precursor such as monophenol of catechol and resorcinol.

Phenol Contents and Antioxidant Activity of Sonokeling (Dalbergia latifolia Roxb) Wood Masendra Masendra; Denny Irawati; Alamratush Shoolichah Ridlo; Ganis Lukmandaru
Wood Research Journal Vol 11, No 1 (2020): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2020.11.1.27-34

Dalbergia latifolia or sonokeling is a native species of Java, Indonesia, used as an important wood for furniture and building materials, due to the high of durability and beautiful color. This study, therefore, aim to investigate the phenol composition, represented by total phenolic, flavonoid, and flavanol content, as well as antioxidant activity, conducted by DPPH (1, 1-diphenyl-2-picrylhydrazyl) method on Dalbergia latifolia wood. The sample was extracted using ethanol-toluene solvent in a Soxhlet apparatus, and subsequently subjected to column chromatography. This treatment yielded 12 fractions, which were then evaluated for phenol contents and antioxidant activity. The results showed a high antioxidant activity and total phenolic content in Fr.1- Fr.3, while latifolin was detected and characterized by GC-MS and a literature comparison. Therefore, it was established that the antioxidant activity of D. latifolia wood extractives properly correlated with the total phenolic, but not with the total flavonoid and flavanol contents.

Antifungal Activity of Triterpenoids and Steroids Isolated from Pinus merkusii Bark Against Phanerochaete chrysosporium Masendra Masendra; Brandon Arsito Verick Purba; Ganis Lukmandaru
Wood Research Journal Vol 11, No 2 (2020): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2020.11.2.65-71

The outer part of a tree, known as the bark, protects the internal tissues from external conditions and attacks from microorganisms. Its antifungal activities are due to the presence of chemicals in this bark. This study aimed at evaluating the toxicity of triterpenoids and steroids from the bark of Pinus merkusii against Phanerochaete chrysosporium (white-rot). The triterpenoids and steroids were isolated from n-hexane extract of the bark through column chromatography. Then, the antifungal activity was evaluated by measuring the growth rate of the fungus on potato dextrose agar (PDA) medium in a Petri dish. The isolation resulted in three triterpenoids (3β- methoxyserratt-14-en-21-one, serrate-14-en-3β,21β –diol, 3α,21β- dimethoxy-D14-serratene) and two steroids (β-sitosterol, Stigmast-4-en-3-one). There was more of antifungal activity with β-sitosterol and serrate-14-en-3β,21β –diol, due to presence of hydroxyl bond and their hydrophobicity.

Antifungal and Antioxidant Activities of Lipophilic Compounds from Swietenia mahagoni (l.) Jacq. Leaves Masendra Masendra; Brandon Aristo Verick Purba; Rizki Arisandi; Ganis Lukmandaru
Wood Research Journal Vol 6, No 2 (2015): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2015.6.2.62-68

The investigation on the n-hexane lipophilic extractives of Swietenia mahagoni, alongside the antifungal and antioxidant properties was conducted. The leaf sample was collected from 2- and 3-years-old trees in Perhutani enterprise of Temanggung, Central Java, Indonesia. In addition, the antifungal activity was tested using the white-rot of Phanerochaete chrysosporium, while the antioxidant property involved the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The results show antifungal of 28.6% and 37.5% growth inhibition in 2- and 3 -years-old leaf, respectively, while 21.7% and 25.4% antioxidant activity were also recorded. Furthermore, the GC-MS results showed the domination of S. mahagoni leaf by fatty acids and hydo-carbons, while the terpenoids and steroids were in minor quantities. These components possibly exhibit growth inhibitory and antioxidant effects against P. chrysosporium and DPPH radicals, respectively.

Extractives Contributing to the Color of Swietenia macrophylla King Bark Masendra Masendra; Rizki Arisandi; Brandon Aristo Verick Purba; Fuad Sumantri; Fatra Valahatul Ihda; Fatimah Zulaikha Wati; Ganis Lukmandaru
Wood Research Journal Vol 11, No 1 (2020): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2020.11.1.20-26

The bark of Swietenia machrophylla is colored dark red, and it is correlated with the extractives constituents such as phenolic compounds. This study, therefore, aims to investigate extractives from the inner and outer bark of S. machrophylla and their effects to color properties. The results showed that the extractive content in the inner bark was higher than the outer except for hot water solubles. In addition, the polyphenols and sugar levels from inner to outer bark were increased, except in the soluble-sugar of hot water extractive. The highest correlation between the absorbance of methanol, hot water-soluble extracts, and total polyphenols were observed using the visual spectrophotometer. The extractives that contributed to the bark's color were indicated from flavonoids with a precursor such as monophenol of catechol and resorcinol.

Phenol Contents and Antioxidant Activity of Sonokeling (Dalbergia latifolia Roxb) Wood Masendra Masendra; Denny Irawati; Alamratush Shoolichah Ridlo; Ganis Lukmandaru
Wood Research Journal Vol 11, No 1 (2020): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2020.11.1.27-34

Dalbergia latifolia or sonokeling is a native species of Java, Indonesia, used as an important wood for furniture and building materials, due to the high of durability and beautiful color. This study, therefore, aim to investigate the phenol composition, represented by total phenolic, flavonoid, and flavanol content, as well as antioxidant activity, conducted by DPPH (1, 1-diphenyl-2-picrylhydrazyl) method on Dalbergia latifolia wood. The sample was extracted using ethanol-toluene solvent in a Soxhlet apparatus, and subsequently subjected to column chromatography. This treatment yielded 12 fractions, which were then evaluated for phenol contents and antioxidant activity. The results showed a high antioxidant activity and total phenolic content in Fr.1- Fr.3, while latifolin was detected and characterized by GC-MS and a literature comparison. Therefore, it was established that the antioxidant activity of D. latifolia wood extractives properly correlated with the total phenolic, but not with the total flavonoid and flavanol contents.

Investigation of Flavonoid Extractives and their Contribution to Color of Dalbergia latifolia Roxb Wood Masendra Masendra; Brandon Aristo Verick Purba; Denny Irawati; Ganis Lukmandaru
Wood Research Journal Vol 12, No 2 (2021): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2021.12.2.53-60

Dalbergia latifolia wood is a beautiful wood with color of dark purple to dark brown and rich in flavonoid compounds. This study aimed to investigate the presence of flavonoid compounds from D. latifolia wood and their contribution to its color. The D. latifolia wood sample was powdered and extracted with ethanol-toluene (1/2, v/v). The ethanol- toluene extract then was column chromatographed to obtain fraction 1 to fraction 12. Methylation was conducted to each fraction to detect flavonoid compounds by GC-MS. The results of GC-MS analysis showed that ethanol- toluene extract gave Fr- 1- Fr. 3 as soluble flavonoids (34.2%) and Fr. 4- Fr. 12 as insoluble polymers (62.6%). Furthermore, 12 flavonoids i.e latifolin, dalbergiphenol, chalcone, dalbergin, and their derivatives were characterised and these compounds might be significance in contributing to color of D. latifolia wood.

Phenolics Content and DPPH Free Radical Scavenging Activity of Dalbergia latifolia leaf ethanolic extract Masendra Masendra; Agus Ngadianto; Ridla Arifriana; Greitta Kusuma Dewi; Eko Prasetyo; Muhammad Rafly Nugraha; Saffana Ahda Labiba; Fatimah Ayu Warahapsari; Brandon Aristo Verick Purba; Ganis Lukmandaru
Wood Research Journal Vol 13, No 2 (2022): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2022.13.2.56-62

Dalbergia latifolia is one of the famous species that naturally grows in Java, Indonesia. The wood of this species is purple in color and the leaf is utilized as forage. The purpose of this study is to determine the bioactivity and phenol contents of D. latifolia leaf ethanolic extract. The antioxidant activity and anti-termite activity were measured through the DPPH method and Neotermes bosei activity. Furthermore, phenol contents were determined through colorimetric method and ethanolic extract constituents were identified by GC-MS. The DPPH inhibition of ethanol extract of D. latifolia leaf exhibited 138.20±2.14 µg/ml. While the measurement of total phenol and total flavonoid content of D. latifolia leaf showed a value of 192.67±9.41 mg GAE/ g and 55.23±5.11 mg QE/ g of dried extract sample. The termiticidal activity of ethanolic extract showed low activity. The GC-MS detection showed fatty acids as dominant compounds. The inhibition of DPPH by D. latifolia leaf ethanol extract in this study suggested this leaf is potent as antioxidant agents.

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