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All Journal Agrivet Techno LPPM Dharma: Jurnal Pengabdian Masyarakat
Endah Wahyurini, Endah
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Agriculture, Biological Sciences & Forestry Social Sciences

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Journal : Techno LPPM

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CALLUS REGENERATION OF Chrysanthemum AFTER GAMMA RAY IRRADIATION FOR THE RESILIENCE OF MEDIUM PLAIN Wijayani, Ari; Muafi, Muafi; Wahyurini, Endah; Lestari, Rina Sri
Journal TECHNO Vol. 1 No. 1 (2015)
Publisher : Universitas Pembangunan Nasional Veteran Yogayakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/journal techno.v1i1.1502

Abstract

Development of chrysanthemum plants in the medium plains is still facing obstacles due tounfavorable climate. Until today, the availability of tolerant chrysanthemum seeds grown in the mediumplains is not maximized so that the necessary effort in order to increase the genetic diversity as thematerial selection to obtain tolerant chrysanthemums grown in medium plain. This research is beingconducted in order to follow up the problems of availability of tolerant chrysanthemums seeds grown inthe medium plains at several stages. Stage one is the induction of callus after gamma ray irradiation invitro. This research has been conducted in tissue culture laboratory of the Faculty of Agriculture UPN"Veteran" Yogyakarta from February to June 2015. Tested regeneration media is ½ MS with the additionof IAA 0.1 mg/l; 0.2 mg/l; 0.3 mg/l; 0.4 mg/l and 0.5 mg/l. The results showed that ½MS regenerationmedia with the addition of IAA 0.3 mg/l spur sprouts emerge percentage (100%); while growing sprouts(8.67 days), height are about sprouts (3:10 cm) and number of sprout (7:00). Regeneration mediumTreatment with kinetin 2 mg/l + IAA 0.5 mg/l gives the number of root growth (13) and root length (4:07cm)Keywords: Chrysanthemum, tolerant medium plains, in vitro selection, gamma ray irradiation,
In Vitro Micro-Cutting Of Vanilla (Vanilla Planifolia Andrews.) In Different Naa And Bap Prabaninggar, Rizki Amalia; Sasmita, Ellen Rosyelina; Wahyurini, Endah
Journal TECHNO Vol. 7 No. 1 (2021): Mei
Publisher : Universitas Pembangunan Nasional Veteran Yogayakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/journal techno.v7i1.5429

Abstract

Vanilla is one of the spice plant that has a high selling value. The problem with conventional propagation of vanilla by stem cuttings is the attack of stem rot disease caused by Fusarium oxisporum f sp. and limited planting material, therefore can be overcome by tissue culture techniques. This study aims to examine the interaction between the various concentrations of NAA and BAP, obtain the most appropriate concentrations of NAA and BAP for vanilla’s micro-cutting. The study used a Completely Randomized Design (CRD) method. The first factor is the concentration of NAA consisting of N1: 0,5 ppm, N2: 1 ppm, and N3: 1,5 ppm. The second factor is the concentration of BAP consisting of B1: 1 ppm, B2: 2 ppm, and B3: 3 ppm. Observation data were analyzed using Analysis of Variance (ANOVA) and  Duncan's Multiple Range Test (DMRT) at 5% level. The results showed that there was no interaction between the treatment of NAA and BAP concentrations on vanilla micro-cutting. The use of 1 ppm NAA gave the best results on root length. BAP at all concentrations gave the same response on parameters of time to grow buds, live percentage, number of shoots, number of roots, number of leaves, root length, and dry weight of plantlets.
RESISTANCE AND POTENTIAL YIELD TEST OF ACCESSION TOMATO DETERMINATE HYBRID (F1) TO TOMATO YELLOW LEAF CURL VIRUS (TYLCV) Sari, Chitia Novita; Lagiman, Lagiman; Wahyurini, Endah
Journal TECHNO Vol. 7 No. 2 (2021): November
Publisher : Universitas Pembangunan Nasional Veteran Yogayakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/journal techno.v7i2.6228

Abstract

This study aimed to determine the resistance and yield of accessionized determinate hybrid tomatoes (F1) against Tomato Yellow Leaf Curl Virus (TYLCV), and to obtain accessions of determinate tomatoes that are resistant to TYLCV and have high yields.This research method used Completely Randomized Design (CRD) to test the resistance of tomato accessions from TYLCV attack. The second stage of the experiment used a Completely Randomized Block Design (CRBD) to test the yield of  tomato accession at the tolerance level forTYLCV resistance. The treatments consisted of 7 F1 tomato hybrids, namely T-26, T-41, T-121, T-122, T-165, T-175, T-187, and 3 control hybrid namely T-90, Liontin and Betavila. The data obtained were analyzed using analysis of variance (ANOVA) then followed by DMRT (Duncan's Multiple Range Test) at a significance level of 5%. The results showed that the control hybrid T-90 was resistant and the T-122 hybrid was moderately resistant to TYLCV (Experiments stages I and II). The seven hybrids tested (T-26, T-41, T-121, T-122, T-165, T-175, and T-187) had the same weight of fruits as the TYLCV resistant control hybrid (T-90). The superior hybrids on the parameters of weight of fruits, weight per fruits, number of fruit, and fruit diameter were T-122 and T-175. Hybrid (F1) which has resistance to TYLCV and high yield is T-122.
Co-Authors A Wijayani Amalia Nurul Huda Ami Suryawati Bambang Sugiarto Bambang Supriyanta, Bambang Dhiani Dyahjatmayanti Guntoro, Yuan Cahyo Hanif, Muhammad Arkanuddin Hendro Widjanarko KRT Nur Suhascaryo Lagiman, Lagiman Muafi Nailan Nabila Prabaninggar, Rizki Amalia Rima Margareta Retnyo Gumelar Rina Sri Lestari, Rina Sri Santosa Utomo, Humam Sari, Chitia Novita Sasmita, Ellen Rosyelina Suranto Suranto Suratna, Suratna