Fitriyatun Nisa, Fitriyatun
Department of Biology, Faculty of Mathematics and Natural Science, Universitas Riau, Indonesia, Kampus Bina Widya, Jl. HR Soebrantas, Panam, Pekanbaru 28293, Riau

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An Analysis of Partial DNA Sequence of Meisa1 Gene on Sweet and Bitter Cassavas (Manihot esculenta Crantz.) Roslim, Dewi Indriyani; Nisa, Fitriyatun; Herman, Herman
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5365

Abstract

Sweet and bitter taste on cassava tuber is affected by starch metabolisms. Meisa1 gene is a gene in cassava (Manihot esculenta Crantz.) encoding isoamylase1 enzyme involved in starch metabolisms. This study aimed to analyze partial DNA sequences of Meisa1 gene on sweet and bitter cassavas collected by Genetics Laboratory, Department Biology, Faculty of Mathematics and Natural Sciences, Riau University, Indonesia. Methods included total DNA extraction from fresh young leaves of cassava using CTAB buffer, polymerase chain reaction (PCR), electrophoresis, and sequencing. The obtained data were analyzed using MEGA software version 5. The results showed that there were nucleotide variations in the intron region, not in the exon region. The variations were caused by the transition substitution mutation (35.39%) and transversion substitution mutation (64.61%). The genetic distance range between seven cassava genotypes was approximately 0% to 11%. Partial DNA sequence variations of Meisa1 gene located in intron region were unable to cluster seven cassava genotypes separately into two groups based on tuber taste.How to CiteRoslim, D., Nisa, F., & Herman, H. (2016). An Analysis of Partial DNA Sequence of Meisa1 Gene on Sweet and Bitter Cassavas (Manihot esculenta Crantz.). Biosaintifika: Journal of Biology & Biology Education, 8(1) 103-110.
Improving Student Learning Outcomes Through The Wordwall-Assisted Problem Based Learning Model on The Human Digestive Organs Nisa, Fitriyatun; Rochmah, Eliya; Putra, Nugraha Permana
Jurnal Ilmiah Global Education Vol. 6 No. 4 (2025): JURNAL ILMIAH GLOBAL EDUCATION
Publisher : LPPM Institut Pendidikan Nusantara Global

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/jige.v6i4.4435

Abstract

This study aims to improve student learning outcomes through the application of problem-based learning models assisted by wordwall media on the material of human digestive organs in grade V of SDN 2 Lemah Mekar. The method used is classroom action research (CAR) through two cycles. Each cycle consists of the stages of digestion, implementation, observation and reflection. The results of the study showed an increase in learning outcomes from the pre-cycle with a percentage of 31% increasing to 61.54% in cycle I and reaching 92.31% in cycle II. The problem-based learning model provides opportunities for students to be active in solving problems and discussing, while the wordwall media makes learning more interesting and interactive. Thus, the application of the problem-based learning model assisted by wordwall media is proven to be effective in improving student learning outcomes on the material of human digestive organs.